Brazilian Legislation Research Articles

Groundwater Contamination by Gas Stations in Two Eastern Amazonian Towns (Northern Brazil)

The present study analyzed the presence of the principal volatile compounds of the BTEX type (benzene, toluene, ethylbenzene, and xylene [o-, m- and p-xylene]) in samples of water from wells located at residences and gas stations in two Amazonian towns—Tracuateua and Augusto Corrêa—in the Amazon region of northern Brazil. This innovative study is extremely relevant to the Amazonian towns surveyed, given that they lack systematic policies for the environmental control of gas stations and any municipal regulations on the quality of water destined for human consumption. A combination of mass spectrometry (MS) and gas chromatography (CG) techniques was applied to analyze these contaminants in 150 samples of local groundwater collected between 2020 and 2024. One of the four BTEX compounds (toluene) was identified in seven of the samples collected (4.66% of the total) at concentrations of 0.14–2.10 µg L−1. The concentrations of contaminants were low, in general. None of the water samples analyzed here presented any critical loss of water quality for human consumption according to the Brazilian legislation concerning BTEX concentrations. Neither of the two towns surveyed in the present study has remediation programs for environmental contamination. The GC-MS approach produced satisfactory results for the assessment of the contamination of underground water reserves by gas stations in both study towns. Further research (e.g., geophysical methods) will be necessary to determine the source of the contamination and its connection with the levels of toluene identified in the underground water sampled in these Amazonian towns.

Evaluation of the Nutritional Quality and Shelf Life of Fermented Processed Sheep Salami Inoculated with Lactobacillus casei and Lactobacillus paracasei

The aim of this study was to produce two fermented processed foods made from sheep meat, one of them inoculated with the probiotic Lactobacillus casei and the other with Lactobacillus paracasei, evaluating their microbial growth, viability, and the efficiency of these microorganisms as bioconservants. To that effect, physicochemical, physical, and microbiological analyses were carried out, as well as a count of lactic acid bacteria (LAB). The results regarding the physicochemical and physical characteristics were in accordance with Brazilian legislation, except for the humidity and carbohydrate analysis and water activity, the values of which were not in accordance with the legislation. However, the microbiological results regarding the pathogenic microorganisms’ growth were within the standard established in the legislation until the end of the time of analysis. Regarding the presence of probiotics, both fermented processed foods had values higher than 107 CFU/g relative to LAB count until the limit of 84 days. From these results, it can be concluded that the probiotics are viable, showing bioconservative activity and proper consumption conditions, since they are in conformity with the legislation.

Navigating the transition: The impacts of electricity tariff regulation on distributed generation in Brazil

Energy transition presents new challenges in electricity regulation, notably Distributed Generation (DG) tariffs. In Brazil, DG capacity surged 18 times between 2021 and 2023. The impact of recent Brazilian DG legislation (Law 14300/2022; Normative Resolution 1059/2023) on the market remains uncertain. Our analysis, from technical, social, and financial perspectives, focusing on units with up to 5 MW of installed capacity, reveals persisting and increasingly unsustainable cross-subsidy schemes. While new rules mitigate the risk of a “utility death spiral,” misguided regulations may spur DG adoption and hike electricity tariffs. Revising subsidy regulations can mitigate adverse social and financial repercussions.

The application of Law 12.990/2014 in the public tenders of a new Brazilian Federal University: a documentary research

Brazilian legislation determines the reserve of 20% (twenty percent) of vacancies in public tenders for black people. However, difficulties are noted in federal public institutions for the effective execution of the regulations in their tenders. The object of this study was to analyze the process of implementation of Law 12.990/2014 in a new Federal University in relation to the selection, execution and maintenance of black servers. To this end, a documentary research was carried out, also using as an investigation strategy the method of content analysis of Bardin (2020), complemented by similarity analysis made available by the IRAMUTEQ software. The proposal investigated 24 public tender notices for the filling of effective positions, between the years 2014 and 2023. The results showed that the process of implementation of Law 12,990/2014 in the Federal Institution of Higher Education (FIHE) studied presented biases in the application of the standard in its tender notices. It is concluded that, despite the difficulties in the data collection stage, it was possible to understand the methodologies used – and their weaknesses – for the application of Law 12.990/2014 in the public tenders carried out by the FIHE analyzed.

Direitos das trabalhadoras gestantes, adotantes e lactantes no Brasil: legislação aplicada

Due to its complexity, the Brazilian legislation requires continuous study from legal practitioners to ensure proper application. The protective rules for maternity, including pregnancy, adoption, and lactation have gradually been increased over the years, in compliance with Article 7 of the Brazilian Constitution, which encourages the emergence of new social rights. These rights can be stratified into those that arise with pregnancy, those that develop during the course of gestation, and those that protect the child at the beginning of its life outside the womb. Additionally, there are rights pertinent to new situations or aimed at increasing the father’s participation during this initial phase.

Metal-contaminated sediment toxicity in a highly impacted Neotropical river: Insights from zebrafish embryo toxicity assays

The Fundão dam collapse was one of the largest mining-related disasters globally. It resulted in the release of mining tailings containing heavy metals, which contaminated the Doce River in southeastern Brazil. This study assessed the effects of acute exposure of Danio rerio embryos to sediments contaminated by mine tailings six years after the Fundão dam collapse. The study sites included P2, P3, and P4 in the upper Doce River, as well as site P1 on the Piranga River, an uncontaminated river. Sediment samples were analyzed for 10 metals/metalloid by atomic absorption spectrometry. In the assays, embryos were exposed to sediment from P1–P4 sites, and uncontaminated quartz was used as control sediment. Various biomarkers were applied to assess biological responses, and the integrated biomarker response (IBR) index was calculated for each site. Sediment samples revealed elevated levels of As, Cr, Cu, Hg, and Ni beyond Brazilian legislation limits. At 96-h exposure, embryo mortality rates exceeded 20% in P1, P2, and P3, higher than the control and P4 (p < 0.0001). Hatching rates ranged from 60 to 80% in P1, P2, and P3, lower than the control and P4 (p < 0.001). Larvae exposed to P2 sediment (closest to the Fundão dam) exhibited skeletal, physiological, and sensory malformations. Neurotoxicity was indicated by increased acetylcholinesterase activity and reduced spontaneous movements in embryos exposed to Doce River sediment. Contamination also increased metallothionein and heat shock protein 70 levels, along with changes in cell proliferation and apoptosis. Principal component analysis showed a good correlation between metals/metalloid in the sediment and larval morphometric endpoints. The IBR index highlighted suitable biomarkers for monitoring metal contamination in fish embryos. Overall, our findings suggest that sediment toxicity following the Fundão dam failure may compromise the sustainability of fish communities in the Doce River.

Naturally Colored Ice Creams Enriched with C-Phycocyanin and Spirulina Residual Biomass: Development of a Fermented, Antioxidant, Tasty and Stable Food Product

Naturally colored fermented foods currently represent the trend toward a global demand for healthier products. This work produced naturally blue and green ice creams using C-phycocyanin (C-PC) and spirulina residual biomass (RB). The ice creams were assessed based on microbiological analysis, color stability over 6 months, antioxidant activity before and after in vitro digestion, and sensory evaluation. Considering the microorganisms that must be analyzed in accordance with Brazilian legislation, no growth was detected during the storage period. L*, a*, and b* were maintained according to the expected colors. The blue color was intoned over the shelf life (SC-PC *b −9.46 to −19.44 and MC-PC *b from −9.87 to −18.04). The antioxidant activity of the fermented ice creams SC-PC and SRB increased from 15.4 to 41.3 and from 15.3 to 38.0 µM TE/g, respectively, after bioaccessibility analysis. The C-PC ice cream’s appearance received the highest rating, with 70.26% of volunteers expressing a strong preference, highlighting its attractiveness. However, there were no significant differences compared to control samples in the global acceptance. The RB ice cream presented lower results for flavor but moderate acceptance. Thus, these fermented ice creams presented color stability over 6 months, and their antioxidant activity increased after in vitro digestion, highlighting their biological potential.

The impact of blockchain on Brazilian public procurement processes from the perspective of transaction costs: scenarios as perceived by experts

Purpose This paper aims to explore the impacts of a blockchain network implementation to support purchasing processes of a Brazilian public organization. Design/methodology/approach The Grumbach method was used to build the scenarios. Five experts with knowledge in blockchain and experience in public procurement were consulted on 20 possible preliminary events, defining their probability of occurrence and relevance. The data obtained were processed in Puma software, which returned a selection of ten definitive events, based on probability, relevance and standard deviation indicators, generating a map of prospective scenarios. Findings Three following scenarios are shown, the ideal scenario, the one with greater implantation benefits and fewer complications; the trend scenario, more likely to occur under current conditions; and the most likely scenario of occurrence, according to experts. The results indicated which simulated events are drivers (motives), and which are influenced (dependent). They were categorized as opportunities or threats to the deployment of the technology. Research limitations/implications Although public procurement processes are standardized by Brazilian legislation, new events may arise from the replication of the model in different organizations. The research revealed the need for practical testing in a simulated public procurement environment. Originality/value The article explores the interaction between disruptive network technology and processes linked to public sector efficiency. Studies on electronic government point to the future of public management.

Carbon trading

From the analysis of Brazilian legislation, it can be concluded that Brazil needs to move forward when it comes to legislation, both at federal level, because it does not have its own regulation, and also along the 27 states, which do not approach the topic and it does, the acts are confused and flawed, mixing payment for environmental services and carbon credit commercialization.

Using social media image to identify threatened elasmobranch species caught by a small-scale fishery in a data-poor area.

Sharing fishing activity via social media attracts millions of views. The data from these shares includes key information for a fisheries database such as the species present in the catches and in many cases the number of specimens caught. Many countries in the Global South do not have official information about basic data on fishing activity, especially small-scale activity. In many of such countries like Brazil, overfishing poses a significant threat to numerous threatened species, including elasmobranchs, due to the frailty of fisheries management and law enforcement. The lack of a national (Brazil) data collection program makes sharing data on social media a potential source of fisheries related information. Hence, this study aimed to verify the potential of social media data to describe artisanal fishing and identify elasmobranch species caught by a small-scale fleet in a data-poor Equatorial Southwest Atlantic region. The identification of elasmobranch species was made based on 250 videos shared by local fishers publicly on social media (Youtube™). Among these, sharks and rays were observed in 19 (8%) and 42 (17%) videos, respectively. A total of 98 specimens were recorded, of which 80% could be identified to a species level. Most of the records comprised the stingrays Hypanus berthalutzae and Hypanus guttatus, while Ginglymostoma cirratum was the most recorded shark. More than half of the elasmobranch species recorded are listed at some level of threat according to the IUCN Red List. According to the Brazilian legislation, at least 20% of the species identified in these videos are considered Endangered (EN) or Critically Endangered (CR). Therefore, fishing activity shared via social media can be considered effective in identifying threatened elasmobranch species, as well as providing data on species occurrence, number of specimens, or rare sightings. It is suggested that fishing activity shared via social media be used in parallel with traditional methods (e.g. on-board data and landing observations) to obtain fisheries information and data about endangered species in data poor areas.

Monitoring and Assessment of the Quality of Chlorinated Water in Schools

All water destined for human consumption must be subjected to disinfection processes via chlorination with the aim of eliminating the risk of the transmission of waterborne diseases. However, the treatment of water using this method leads to the formation of trihalomethanes (THMs), which are toxic compounds that may be ingested, inhaled, or absorbed by the skin, increasing the risk of carcinogenic and mutagenic processes. High trihalomethane concentrations in water may be directly related to physicochemical properties, such as temperature, pH, hardness, and potentially toxic metal concentrations. In this work, physicochemical characterization was performed and water quality was assessed with regard to the presence of trihalomethanes in 17 samples collected from points before and after storage in reservoirs. Atomic absorption spectroscopy to determine potentially toxic metals and the measurement of physicochemical properties demonstrated that all results were in accordance with the standards established by Brazilian legislation. Gas chromatography–mass spectrometry was used to determine levels of trihalomethanes in the water samples (trichloromethane, bromodichloromethane, chlorodibromomethane, and bromoform), which were also in accordance with the limits established by Brazilian legislation. Hierarchical clustering on principal components confirmed changes in the water quality depending on location. This work constitutes a paradigm for future studies on the monitoring of toxic organic compounds in water to avoid health problems in humans and animals.

The Importance of Analysis of Judicial Circumstances in the Sentence Dosimetry Process

The Constitution plays an important role as a point of reference for the critical analysis of the criminal system, not only because it derives from the legal assets susceptible to punitive protection, but also and among other reasons, because the principles enshrined as a government norm in the Penal Code governing The imposition of sanctions is a clear development of the constitutional text and doctrine that the Constitutional Court has been building with its pronouncements, in the form that criminal law must acquire. The dosimetry of punishment in Brazil takes a three-step approach, pushing the system of fixed sanctions implemented by the legislature or the full discretion of the judge, promoting, on the other hand, a distribution of functions between the two, as he chooses the minimum and maximum sentence levels and dosage parameters, and this determines the sanction in the specific case from the legal criteria. In this sense, the present research aims to analyze the importance of analyzing judicial circumstances in the process of penalty dosimetry. The specific objectives are: to present a brief history of criminal legislation in Brazil, to review the most recent literature on the application of the penalty and its principles, to analyze the relationship between the dosimetry of the penalty and judicial circumstances and to point out cases in the jurisprudence that materialize the revised theory. Thus, given the relevance of the theme in the current legal scenario, the research is justified by discussing a contemporary theme. Through research on the principles of exceptionality, state of innocence, motivation of decisions and proportionality and the dosimetry of the penalty, possible judgments of the judge that relate the dosimetry of the penalty with the principles of human rights will be discussed. The judge should take charge of the evidence produced in relation to the facts that, in his opinion, influence the minor or major measure of the exact penalty to be imposed. The lack of motivation currently constitutes a violation of the constitutional guarantee of due process and absolute grounds for invalidity under the Criminal Procedure Code.

Bee honey of the Pajeú hinterland, Pernambuco, Brazil: Physicochemical characterization and biological activity

The state of Pernambuco, in the northeast Brazil, is one the biggest bee honey producers in the country, especially in semiarid microregions called “Sertão", or hinterlands. Its unique biome characterized by xerophyte Caatinga vegetation presents a unique flora with many medicinal properties. One of these microregions, Sertão do Pajeú, has stood out for its growing honey production. The present work reported for the first time a characterization of this honey and evaluated its potential for the production of alternative nutraceutical products. Samples from Sertão do Pajeú and two other regions of the State were collected and subjected to analyses as recommended by the Brazilian legislation. As the most striking characteristics, honeys from this microregion showed darker colour, higher content of phenolic compounds and elevated antioxidant activity than that from other locations. The high content of 5-HMF, together with high diastase activity, indicated that it is an intrinsic property of this honey. And finally, fermented worts containing the probiotic yeast Saccharomyces boulardii were supplemented with honey and sucrose as reference. The results showed that samples stocked in the presence of honey presented high level of cell longevity over 60 days of storage under refrigeration. In addition, those cells became very tolerant to the simulation of the gastrointestinal tract. Therefore, this honey greatly increased the probiotic potential of the yeast. This shows the nutraceutical potential of honey from Sertão do Pajeú.

PARENTAL ALIENATION IN THE FAMILY LEGAL PROCESS: The importance of the role of legal psychology in its proof

The aim of this study is to analyze how parental alienation is harmful in the family legal process, as well as to describe the importance of the psychologist's role in proving parental alienation. Legal psychology assists the judge in understanding cases involving the custody of children and adolescents, protecting their interests. To do this, it was necessary to conceptualize what Brazilian legislation and the Psychologists' Code of Conduct deal with regarding psychological expertise in civil proceedings that deal with parental alienation. The methodology adopted was a bibliographic, descriptive and analytical review, through the survey of reference articles on the topic, visits to government websites and survey of jurisprudence on the topic. As a conclusion of the research, it is observed that the psychologist is essential for identifying or not the conduct of parental alienation, considering that these processes that deal with this conduct often do not have clear evidence of the practice.

Employment discrimination and labor market protections for sexual minorities in Brazil

This study estimates the impacts of an employment non-discrimination act (ENDA) for sexual minorities in Brazil. We contribute new evidence on ENDA impacts for sexual minorities in a distinct context from the current literature. Additionally, this is one of few studies to document labor market gaps by sexual orientation in Latin America. In this paper, we first present evidence of labor market gaps in employment, formal sector employment, hours worked, income and hourly earnings for lesbian and gay individuals in Brazil. For the same groups, we then estimate the impact of Brazilian legislation forbidding employment discrimination based on sexual orientation on these same labor market outcomes. Regarding initial pre-policy labor market gaps, conditioning on individual and work-related controls, we see that lesbian women experience wage premiums compared to straight women, while gay men experience wage penalties compared to straight men. We then estimate robust evidence that the implementation of employment protections increased income for gay men. For lesbian women, we estimate evidence of post-policy reductions in employment, but higher income and hourly earnings for those still working. Focusing on a Latin American country, we find many impacts consistent with the more recent existing evidence on ENDAs for sexual minorities.

Factors for Implementing Medical Cannabis Cultivation in Brazil

Abstract: The medicinal properties of the Cannabis genus are evident and many studies indicate its usefulness to treat many diseases. However, the production of Cannabis-based products has been hampered by its criminalization. Currently its cultivation is not allowed in Brazil, except in specific situations. It increases the price of Cannabis-based products, as it requires the importation of inputs, making it difficult for most of the population to access such medicines. In this context, this review presents an overview of Brazilian legislation on medical Cannabis and the current market for Cannabis products in Brazil. The main goal is to encourage the cultivation of medical Cannabis and the distribution of its products by the Brazilian Unified Health System. In addition, it presents recommendations to implement cultivation based on a bibliographic survey carried out in scientific databases and clinical evidence of the effectiveness of Cannabis in treating patients suffering from several diseases and conditions, although more complete studies are needed to confirm both risk-benefit relationship and dose to be administered. After searching for legislation and bills on government websites, it became evident that there was a need for changes in Brazilian legislation to enable cultivation in Brazil. However, there are several proposals for legislation in progress, and Brazil can significantly benefit from this therapeutic option.

CONTRIBUTIONS OF BIOPHILIA TO SUSTAINABLE DEVELOPMENT

Addressing the application of biophilia in urban areas, this article aims to analyze the effectiveness of its implementation in promoting sustainable development in its three main dimensions: environmental, economic, and social. The study proposes three specific objectives. Firstly, to highlight the intrinsic connection between humans and nature, examining how this relationship has evolved over time and how the biophilic concept reaffirms it as a fundamental core. Next, to analyze how the 2030 Agenda, a global commitment to sustainable development, relates to biophilic principles, and to assess whether Brazilian legislation is contributing to integrating these principles into urban life in the country. Finally, to investigate and understand how the different dimensions of sustainable development connect to biophilic principles and their impacts on well-being in urban areas, especially in achieving goal 11.7 of the aforementioned Agenda. Considering biophilia as one of the guiding principles of sustainable development, it is concluded that when applied in urban areas, it is a principled conception capable of reconciling urban progress and human relations with nature, providing an increase in individuals' well-being and contributing to the expansion of green areas in urban spaces, as well as collaborating with sustainable precepts. The contribution of this study lies in the need to establish a new paradigm for human interaction with the environment through development that promotes harmonious interaction with nature. To achieve the intended scope, the present research employed the hypothetico-deductive method, starting from the concept of biophilia to analyze whether its principles and guidelines can inspire sustainable actions, involving bibliographic and documentary research.

Critical Analysis of Legal Discourse: A study of the Effectiveness of Brazilian Environmental Legislation

Critical Analysis of Legal Discourse: A study of the Effectiveness of Brazilian Environmental Legislation

Acetic Fermentation of Cagaita Pulp: Technological and Chemical Characteristics

The Brazilian Cerrado region has a rich plant diversity, with fruits that have peculiar and unique sensory characteristics. For these reasons, using these fruits for biotechnological production is a promising alternative, mainly to protect this biome from deforestation and degradation. The production of fermented acetic acid is an option to add value to native fruits and offer the market beverages with better nutritional quality and bioactive compounds. This work aimed to characterize fruits and to develop cagaita (Eugenia dysenterica DC.) acetic fermented beverage. The fruits were subjected to physical-chemical analyses in the first part. Subsequently, different treatments for fermentation were tested using two types of enzymes (amylase and pectinase), two subspecies of Saccharomyces cerevisiae yeast (UFLA CA11 and thermoresistant LNF Angel), and the chaptalization of the must with sucrose (16 °Brix). Alcoholic fermentation was carried out in an incubator with temperature control at 34 ± 1 °C. The pH, total soluble solids, titratable acidity, alcohol content, and density of the fermented products were monitored daily. The chaptalized must with amylase addition and thermoresistant yeast had the best performance during alcoholic fermentation, demonstrating that thermoresistant yeast is an economically advantageous and efficient alternative for the cagaita juice fermentation process. Subsequently, acetic fermentation was carried out using the slow method. Heat-resistant yeast without added enzymes was used to produce cagaita acetic fermented beverages within the parameters of the Brazilian legislation. Furthermore, phenolic compounds and antioxidant activity in the final product were observed. The work demonstrated the possibility of using cagaita fruits in biotechnological processes to produce new food products.

Evaluation of the safety and quality of Brazil nuts (Bertholletia excelsa) using the tools of dna sequencing technology and aflatoxin profile.

Brazil nuts (BNs) result from sustainable extraction and are widely exploited in the Amazon region. Due to the production characteristics in the forest and the nutritional characteristics of these nuts, the occurrence of fungal contamination and the presence of aflatoxins are extensively discussed in the literature as a great aspect of interest and concern. This study aims to evaluate the microbial profile through DNA sequencing and amplification of 16S and ITS genes for bacterial and fungal analysis, respectively, and the presence of mycotoxins using high-performance liquid chromatography with fluorescence detection (HPLC-FD) from different fractions of the nuts processed. The BN samples, harvest A (HA) and harvest B (HB), from two different harvests were collected in an extractive cooperative in the Amazon region for microbiological analysis (from DNA extraction and amplification of 16S genes, bacteria analysis, and ITS for fungi) and mycotoxins (aflatoxins AFB1, AFB2, AFG1, and AFG2) using HPLC-FD/KobraCell®. The samples showed a very different microbiome and aflatoxin profile. Genera such as Rothia (HA) and Cronobacter (HB) were abundant during the analysis of bacteria; as for fungi, the genera Aspergillus, Fusarium, Penicillium, and Alternaria were also considered prevalent in these samples. Soil microorganisms, including those pathogenic and related to inadequate hygienic-sanitary production practices, as well as aflatoxins, were found in the samples. However, they were within the established limits permitted by Brazilian legislation. Nuts have a diverse microbiota and are not restricted to fungi of the genus Aspergillus. The microbiological and toxicological profile can vary significantly within the same nut in the same extraction region and can be exacerbated by global climate changes. Therefore, it is necessary to advance sanitary educational actions by applying good production practices and inspection programs to ensure the sustainability and quality of the BN production chain.