Leaf Extract Research Articles

Mechanistic Wound Healing of Ficus trijuja Leaf Extract and Its Lipid Nanocapsule Supported by Metabolomic Profiling and In Vivo Studies

Mechanistic Wound Healing of Ficus trijuja Leaf Extract and Its Lipid Nanocapsule Supported by Metabolomic Profiling and In Vivo Studies

The Preservation Potential of Utazi and Scent Leaf Extracts against Spoilage Microbes Isolated from Spoilt Water Yam

The Preservation Potential of Utazi and Scent Leaf Extracts against Spoilage Microbes Isolated from Spoilt Water Yam

In-vitro and in-vivo studies of Tridax procumbens leaf extract incorporated bilayer polycaprolactone/polyvinyl alcohol-chitosan electrospun nanofiber for wound dressing application.

This study was an attempt to fabricate an antibacterial wound dressing, which was a bilayered polycaprolactone / polyvinyl alcohol-chitosan (PCL/PVA-CS) nanofibrous membrane. Entrapping ethanolic leaf extract of Tridax procumbens L. (PCL/PVA-CS/Tp). The membrane was prepared using the electrospinning technique to obtain beadless uniform nanofibers. The extract was then infused into the membrane by spraying and exposing to high temperature. in vitro antibacterial activity and cell viability of the membranes were performed. An optimized concentration of 800μg. mL-1 of Tp extract in PCL/PVA-CS/Tp evinced better antibacterial effect on E. coli than S. aureus and also showed rapid wound closure with a positive impact on the viability of L929 cell line. The tissue regeneration efficacy of PCL/PVA-CS/Tp was validated by the experiments on mice models with subcutaneous wounds created using biopsy punch and laser radiation causing burns. Furthermore, in vivo assessments illustrated that the biopsy punch wounds healed more rapidly than laser burn though healing was significant in both. The healing processes such as anti-inflammation and re-epithelialization were observed through histological study. The upregulation of proteins namely VEGF and CD31 along with a decrease in protein levels of Wnt and TGF-β indicated significant wound healing. In conclusion, the bilayered membrane infused with plant extract could be considered a potential material for wound dressing.

Anti-oxidant Effects of Chromatographic Fractions of Abrus precatorius (Linn.) Leaf Extract in Wistar Rats

Aims: Plant antioxidants are considered safe, nutritional, and therapeutic, offering promising solutions to oxidative stress-related health issues. This study explores the antioxidant potential of column fractions derived from the methanol extract of Abrus precatorius, a plant with limited prior evaluation of its bioactive fractions. Study Design: The study used an experimental design. Place and Duration of Study: Orlu LGA, Imo State, Nigeria Methodology: Plant materials were collected from Orlu LGA, Imo State, Nigeria. The dried, powdered leaves were screened for phytochemical constituents using standard methods. Methanol extract was obtained through cold maceration and further fractionated using column chromatography packed with silica gel (60–120 mesh), with elution performed using a gradient mixture of hexane, ethyl acetate, and methanol. The phytochemical analysis confirmed the presence of alkaloids, flavonoids, tannins, cardenolides, and saponins, compounds known for their antioxidative properties. Column chromatography yielded five fractions (F1–F5), which were assessed for antioxidant activity using both in vivo (rat models) and in vitro methods, including 2,2-diphenyl-1-picryl hydrazyl (DPPH) and nitric oxide assays. Results: Notably, fraction F1 demonstrated a significant increase in glutathione and catalase activity, while F4 showed a marked elevation in superoxide dismutase activity in erythrocytes. In liver tissue, F1 and F5 showed exceptional catalase activity, with respective increases of 258 % and 290 %. Fraction F3 exhibited a 290 % increase in superoxide dismutase activity. Furthermore, F3 effectively reduced DPPH radical activity with an IC50 of 0.2 mg/mL, while F4 significantly lowered nitric oxide levels with an IC50 of 0.3 mg/ml. Conclusion: This study highlights the novel identification of potent antioxidant fractions from Abrus precatorius methanol extract, offering valuable insights for developing plant-based therapies for oxidative stress-related disorders such as cardiovascular diseases, diabetes, and neurodegenerative conditions. It underscores the plant's potential as a safe, natural antioxidant source for further pharmacological exploration.

Enhancing the Biological Properties of White Chocolate: Moringa oleifera Leaf Extract as a Natural Functional Ingredient

Moringa oleifera tree is recognised for its high content of bioactive compounds. This work explored the potential of incorporating its leaves or respective extracts into white chocolate to enhance its biological and sensory properties as white chocolate lacks the beneficial compounds found in cocoa. In this study, a phenolic-rich extract was obtained from Moringa oleifera leaf powder, and its biological properties and phenolic composition were characterised. The extract displayed good antioxidant capacity, especially against ABTS radical (IC50 = 162.0 mg/L). Additionally, it exhibited strong inhibitory potential against α-amylase and β-glucosidase, achieving average inhibition rates of 79.9% and 98.0%, respectively. The main phenolic compounds identified included catechin (0.211 mgcompound/gextract), caffeic acid (0.056 mgcompound/gextract), and quercetin (0.031 mgcompound/gextract). White chocolate samples were fortified with 1% and 3% M. oleifera leaf extract, resulting in increased antioxidant properties and oxidative stability. All formulations were microbiologically safe, and the sample containing 3% extract showed the highest DPPH inhibition after 15 days of storage and a higher delay in the autoxidation of lipids over time. The fortification of white chocolate with M. oleifera leaf extract has the potential to transform it into a functional product rich in antioxidants, providing health benefits and increased value.

Mitigating Physiological and Histological Changes Induced by a High-Salt Diet in Male Albino Rats Using Origanum majorana and Olea europaea Leaf Extracts

Mitigating Physiological and Histological Changes Induced by a High-Salt Diet in Male Albino Rats Using Origanum majorana and Olea europaea Leaf Extracts

A Comparative Study on Centella Asiatica‐Mediated Green Synthesis of Silver and Copper Oxide Nanoparticles: Implications for Wound Healing

AbstractConventional wound dressings often lack efficient antimicrobial properties and may exhibit limited bio‐compatibility, necessitating the development of advanced solutions for effective wound healing. Green synthesis of nanoparticles opens the door to a strategy that is efficient in terms of pharmaceutical biotechnology, besides being nontoxic and eco‐friendly. In the present study, Centella asiatica leaf extract conjugated with silver nanoparticles and copper oxide nanoparticles are synthesized and their bioactivity is compared. Gas chromatography mass spectrometry (GC‐MS) results show 4‐butoxy‐1‐Butanol, Phthalic acid, and 2‐propyldecan‐1‐ol as major phytochemicals in Centella asiatica. The green‐synthesized silver nanoparticles (Ag‐NPs) and copper oxide nanoparticles (CuO‐NPs) are characterized using transmission electron microscopy (TEM), X‐ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR). TEM results confirm the circular morphology for Ag‐NPs and elliptical structures for CuO‐NPs, whereas energy dispersive x‐ray (EDX) spectrum confirms the presence of Cu and Ag in their respective samples. XRD spectrum confirms that both the nanoparticles exhibit high crystallinity. Both nanoparticles demonstrate satisfactory radical scavenging activity as confirmed by the 2,2‐Diphenyl‐1‐picrylhydrazyl (DPPH) assay. CuO‐NPs exhibit greater antibacterial activity against Escherichia coli (E. coli) and Methicillin‐resistant Staphylococcus aureus (MRSA) in comparison to the Ag‐NPs evident from the clear zone of inhibition obtained. In conclusion, green synthesized CuO‐NPs prove to have enhanced wound healing activity in the NIH3T3 fibroblast cells than Ag‐NPs.

Total lactic acid bacteria, total dissolved solids, and organoleptic properties of cocogurt with the addition of stevia leaf extracts

The term "cocogurt," a combination of the words "coconut" and "yogurt," is commonly used to describe yogurt prepared with coconut milk. Cocogurt is a lactic acid bacteriafermented beverage made from coconut milk that has similar properties to yogurt. Sugar is used as the carbon source in the majority of commercially available fermented beverages. Customers, particularly diabetics, are demanding that food items have lower calorie levels, so sugar, which is known to have a high concentration of calories, is beginning to be avoided as a sweetener in some products. In the case of cocogurt, replacing sugar with stevia leaf extract could be an alternative solution. However, there has yet to be any reporting on the inclusion. This study aimed to determine the effect of adding stevia leaf extract to the total lactic acid bacteria, total dissolved solids, and organoleptic properties of cocogurt. This study involved four treatments and five replications, with additions of stevia leaf extract at varying concentrations (T0 = 0%, T1 = 0.5%, T2 = 2%, and T3 = 3.5%). The materials used in the research were grated coconut, stevia leaf powder, skim milk, and yogurt starter. According to the study's findings, adding stevia leaf extract in various quantities had a significant impact (p<0.05) on the amount of total lactic acid bacteria, total dissolved solids, sweetness, and acidity of cocogurt. The T0 treatment had the most lactic acid bacteria, with an average of 4.47×108 ±0.125 log CFU while the T3 treatment had the least, with 6.61×107 ±0.20 log CFU. Each treatment had at least 107 CFU of total lactic acid bacteria which is the minimum required by the Indonesian National Standard for fermented beverages. The T0 treatment had the lowest total dissolved solid value of 2.92±0.11°Brix and the T3 treatment had the highest total solid value of 3.26±0.17°Brix. Therefore, it can be concluded that cocogurt made with 0.5% stevia leaf extract contained the optimal amount of total lactic acid bacteria, meeting the requirements of the Indonesian National Standard, and had a moderately sweet and sour flavor in terms of sweetness and acidity.

Ameliorative effect of aqueous leaf extract of Pistacia lentiscus L. against oxaliplatin-induced hepatic injury, oxidative stress, and DNA damage in vitro and in vivo.

The current study aimed to assess the preventive effects of aqueous leaf extract of Pistacia lentiscus (ALEPL) against Oxaliplatin (OXA)-induced DNA damage, hepatic injury, and oxidative stress. The in vitro cytotoxic and genotoxic effects of OXA and ALEPL on HCT116 colon cancer cells were evaluated using the MTT (Tetrazolium salt reduction) assay and comet assay. The in vivo study involved 24 female NMRI (Naval Medical Research Institute) mice that were equally divided into four groups as follows: Control group, ALEPL-treated group (100 mg/kg), OXA-treated group (7 mg/kg), and ALEPL-treated group (100mg/kg) + OXA (7mg/kg). All animals were sacrificed 48 h after OXA treatment. Samples of liver and blood were collected for histopathological, micronucleus, and biochemical analyses. Oxidative stress parameters were also evaluated through non-enzymatic and enzymatic antioxidant activities. Our findings demonstrated that ALEPL contains high phenolic compounds. In the MTT assay, OXA exerted the most potent cytotoxic effect, but ALEPL alone showed no toxic effect in HCT116 cells. Furthermore, OXA administration caused significant DNA fragmentation both in vitro and in vivo, elevated serum biochemical parameters, and confirmed acute liver damage through histopathological observations compared to the control group. OXA exposure also led to a decrease in hepatic glutathione (GSH) and an increase in lipid peroxidation and antioxidant enzyme activities. From the results of our study, ALEPL pretreatment significantly restored the hepatic toxicity and DNA damage as well as the oxidative stress profile induced by OXA.

Overexpression of E. coli formaldehyde metabolic genes pleiotropically promotes Arabidopsis thaliana growth by regulating redox homeostasis.

Formaldehyde (FA) is a hazardous pollutant causing acute and chronic poisoning in humans. While plants provide a natural method of removing FA pollution, their ability to absorb and degrade FA is limited. To improve the ability of plants to degrade FA, we introduced the E. coli FrmA gene into Arabidopsis thaliana alone (FrmAOE lines) or with FrmB (FrmA/BOE lines). The transgenic seedlings had approximately 30 % longer primary roots and a 20 % higher fresh weight than the control plants. The transgenic plants started flowering four days earlier and had about 30 % more kilo-seed weight than the wild type. FrmA/BOE and FrmAOE accumulated 40 % more reactive oxidative species (ROS) in mesophyll protoplasts and leaf tissue than wild-type plants under normal conditions. In the presence of FA, they produced 92 % and 26 % more glutathione (GSH) and 6 % and 4 % more ascorbate (AsA), respectively, compared to wild-type plants and thus scavenged FA-induced ROS more effectively. The degradation efficiency of the transgenic leaf extract for FA was 73 % and 44 % higher than that of the wild type, respectively, which was also emphasized by a 2 %-26 % increase in the activity of antioxidant enzymes such as SOD and APx. By revealing the functional divergence between microbial and plant FA metabolic pathways, our work has not only highlighted the promising pluripotency of microbial genes in promoting normal plant growth and detoxifying organic pollutants simultaneously, but also revealed another layer of complexity of plant defense mechanisms against organic toxins related to ROS scavenging.

A Review of Potentials of Carica Papaya Leaves in Dengue Viral Infection - Insights of Clinical and Preclinical Studies.

One of the most widespread arboviral diseases in the world, dengue virus disease (DVD) is primarily found in tropical and subtropical regions, affecting 129 countries. The main way that the dengue virus (DENV) spreads is through the bite of a female Aedes aegypti mosquito. Symptomatic therapy and supportive care are the primary methods of managing patients with DENV infection as there is currently no approved antiviral medication for this condition. Since the guidelines from the AYUSH Ministry, Government of India, recommend 10 ml of carica papaya leaf extract (CPLE) twice daily for seven days, to treat dengue fever clinically, we plan to review the potential of carica papaya in managing DENV infection.Using terms like dengue, dengue fever, dengue hemorrhagic fever, dengue shock syndrome, and carica papaya, the literature was searched in databases like Medline/PubMed Central/PubMed, Google Scholar, Science Direct, EBSCO, Scopus, Web of Science, EMBASE, Directory of open access journals (DOAJ), and reference lists to find articles relevant to the clinical, in-vivo, in-vitro, and in-silico studies evaluating the efficacy of carica papaya in the management of dengue viral infection. This review included English-language publications that supported the use of carica papaya in the treatment of dengue fever, but it excluded publications that were duplicates.Numerous preclinical and clinical investigations, such as in-vitro, in-vivo, and in-silico studies, have identified carica papaya's anti-dengue potential. The pleiotropic effects of carica papaya, including its anti-thrombocytopenic activity, immunomodulatory effects, and larvicidal property against the Aedes aegypti mosquito species, have also been confirmed by numerous in-vitro and in-vivo studies. These effects can help patients with dengue fever by elevating their platelet count and alleviating other symptoms.To hasten recovery and reduce hospital stays, patients with DENV infection may take carica papaya leaf extract (CPLE) in addition to supportive care and symptomatic treatment. Additional randomized controlled clinical trials would be necessary to confirm the safety and effectiveness of CPLE in patients with DENV infection.

Traditional Uses, Biological Activities, and Phytochemical Profile of Keji Beling (Strobilanthes crispus) Leaf Extract: A Review

Health treatment in Indonesia is diverse, encompassing both modern medicines using synthetic drugs and traditional practices relying on herbal plants. Traditional medicine is often preferred due to its lower cost and perceived lack of side effects. Keji beling (Strobilanthes crispus) is one such herb with potential medicinal properties. This article summarizes information regarding traditional uses, biological activities, phytochemical profiles, and active compounds of keji beling leaf extract. Traditionally, keji beling leaves are consumed as tea. The plant has demonstrated antioxidant, anti-diabetic, anticancer, antitumor, and antibacterial properties. These biological effects are attributed to its phytochemical constituents, including flavonoids, alkaloids, saponins, tannins, and terpenoids. Specific bioactive compounds in keji beling leaf extract comprise silicic acid, polyphenols, glycosides, catechins, rutin, epicatechin, myricetin, luteolin, apigenin, naringenin, and kaempferol.

Development of a Face Wash Formulation Using White Tea (Camellia sinensis) Leaf Extract: Benefits, Mechanism, and Efficacy

Development of a Face Wash Formulation Using White Tea (Camellia sinensis) Leaf Extract: Benefits, Mechanism, and Efficacy

Elucidating the Biological Potential and DNA Protective Activities of Astragalus tokatensis Fisch: GC-MS and LC-HRMS-based Characterization and Molecular Docking Studies.

Astragalus tokatensis is a local endemic species and no study exists on this species. In this study, hexane, dichloromethane, methanol and water extracts were obtained from the parts of root, leaf and flower of A. tokatensis. The phytochemical analyses of hexane and methanol extracts were identified and characterized using gas chromatography-mass spectrometry (GC-MS) and liquid chromatography-high resolution mass spectrometry (LC-HRMS), respectively, for the first time. The total 11 fatty acid and 8 flavonoid derivatives was detected in GC-MS and LC-HRMS analyses, respectively. Total antioxidant levels (TAL) and total oxidant levels (TOL) values of extracts were investigated. TAL and TOL values were given as mM Trolox equiv./L. and μM H2O2 equiv./L, respectively. The α-amylase enzyme activity of all extracts was examined, and it was found that the extracts had no significant effect on the α-amylase activity. Also, the DNA protective activities of the leaf and flower extracts were determined by using gel electrophoresis method and it was seen that all flower extracts had high DNA protective activity. Moreover, Docking assays showed that the limited interactions of sinapinic acid and salicylic acid, the most abundant in the methanol extracts, with α-amylase, supported the weak inhibitory effects observed experimentally in the methanol extracts.

Green Synthesis of Nickel Oxide (Nio) Nanoparticles with Brassica oleracea Var. Capitata F. Rubra, Its Characterisation and Phytochemical Investigation

Most nanoparticles have diameters that lie within 1 to 100 nm. A variety of metal oxide nanoparticles can be synthesised chemically as well as biologically. NiO nanoparticles, which are synthesised chemically, tend to be highly toxic in their effects. In contrast, synthesis by the route of biogenesis or biomimetics is much more favourable than chemical synthesis. The pre-existing properties of purple cabbage are remarkably enhanced by NiO nanoparticles. Nickel oxide (NiO) nanoparticles were synthesised from the leaves of Brassica oleracea var. capitata. f. rubra (purple cabbage) by reducing Nickel nitrate hexahydrate solution for the formation of NiO nanoparticles. The analysis done using a Particle size analyser reveals the spherical morphology and the size of the NiO nanoparticles. The size and shape were studied under a Scanning Electron Microscope, which shows that the nanoparticle ranges from 34.5 nm to 89.6 nm. The formation of NiO nanoparticles was confirmed by the peaks obtained in Raman spectroscopy and UV-DRS analyser. The peaks that ensure the formation of NiO nanoparticles in Raman spectroscopy are 568.40 cm-1, 1129.46 cm-1 and 1379.07 cm-1 and UV-DRS are 265.60 nm and 339.69 nm. Preliminary phytochemical analysis of the aqueous extract of purple cabbage leaves shows that there is an abundance of phytochemical content present in purple cabbage. From the preliminary phytochemical analysis of purple cabbage, it was observed that the aqueous purple cabbage leaf extract contains alkaloids, sterols, flavonoids, coumarin, tannins, cardiac glycosides, anthraquinones, saponins, quinones, and carbohydrates.

Prospective role of lupeol from Pterocarpus santalinus leaf against diabetes: An in vitro, in silico, and in vivo investigation.

Diabetes mellitus (DM) can be treated with various medications. However, individuals in underdeveloped countries may face challenges in using these treatments due to side effects and high costs. Anti-diabetic medications can inhibit enzymes, such as α-amylase, which is responsible for breaking down carbohydrates. In this investigation, 16 phytoconstituents were identified from Pterocarpus santalinus leaf extract through GC-MS analysis, and their significant antioxidant activities were noted. Among these, the pure compound lupeol demonstrated α-amylase inhibition activity of 86.13±1.27% (IC50=58.50±0.83μg/mL) in vitro. Additionally, lupeol showed the highest binding affinity in silico using PyRx and CB-Dock, with values of -9.5 and -9.3kcal/mol, respectively. The in silico analysis also indicated that lupeol possesses acceptable ADMET properties, suggesting its potential as an anti-diabetic drug. A molecular dynamics simulation lasting 500 ns was conducted to evaluate hydrogen bonds, RMSF, RMSD, Rg, and SASA, confirming the stability and integrity of the docking scenarios. In STZ-induced diabetic mice, lupeol significantly reduced blood glucose levels by 70.82% from day 0 to day 28. Furthermore, lupeol markedly decreased total cholesterol (TC), triglycerides (TG), and low-density lipoprotein cholesterol (LDL-C), while improving high-density lipoprotein cholesterol (HDL-C) levels in these mice. The antidiabetic effect of lupeol was further validated through histological examinations of the pancreas, heart, kidney, and liver of treated mice, alongside correlation analysis, CAP, and ANOSIM tests. Based on the findings from the in vitro, in silico, and in vivo investigations, this study concludes that lupeol may have potential anti-diabetic effects through the inhibition of the α-amylase enzyme.

Human Pharmacokinetic Profiling and Comparative Analysis of Mangiferin and Its Monosodium Derivative from Mangifera indica Extracts Using UHPLC-MS/MS with 1H NMR and MALDI-TOF Confirmation

Mangiferin, a glucosyl xanthone, is a plant metabolite with promising nootropic and ergogenic properties. However, its poor and inconsistent systemic bioavailability limits its therapeutic potential. Additionally, the pharmacokinetics of mangiferin from mango leaf extract formulations remain uncharacterized in humans. This study validated a UHPLC-MS/MS method and conducted a human pharmacokinetic study approved by an ethics committee. The bioavailability of mangiferin and its monosodium salt was assessed from two standardized mango leaf extracts: MLE60, standardized to 60% mangiferin but practically insoluble in water, and MLES, the water-soluble monosodium salt form, also standardized to 60%. Twelve participants (six females) received oral doses of each extract in a crossover design with a 7-day washout period. Plasma analysis showed significantly higher AUC and Cmax values with MLES than MLE60, while Tmax and T1/2 were similar. MLES demonstrated a 2.44-fold increase in AUC0–24h compared to MLE60 (p = 0.0029 **), indicating improved bioavailability. This study highlights the salification method as a simple strategy to enhance mangiferin bioavailability, enabling broader applications in beverages and other products where solubility is a limitation.

Phytochemical Screening and Pharmacological Evaluation of Bruguiera gymnorhiza Methanolic Leaf Extract

ABSTRACT Bruguiera gymnorhiza (Rhizophoraceae) is an indigenous mangrove plant with a salient history of traditional uses. This study aimed to explore the phytoconstituents and biological responses of its methanolic (95%) leaf extract (ME). The initial phytochemical screening indicated the presence of alkaloids, carbohydrates, saponins, phenols, and flavonoids. Then, the anthelmintic and antioxidant capabilities were evaluated against Pheretima posthuma (earthworms), DPPH (0.004%), and ABTS cation radical solutions, respectively. Afterward, cardioprotective and hepatoprotective responses were studied in cyclophosphamide-induced myocardial damage and paracetamol-intoxicated liver cirrhosis. The anthelmintic study revealed that ME (50 mg mL−1) had a lower potency (7.45 min) than albendazole (3.63 min) in killing parasites. Furthermore, it exhibited a dose-dependent efficacy (IC50 50.297 and 55.126 µg mL−1) against free radicals in both DPPH and ABTS assay, although less effective than the ascorbic acid (IC50 24.177 and 23.61 µg mL−1). The elevated serum levels of cardiotoxicity indicators (aspartate aminotransferase, lactate dehydrogenase, creatine kinase, troponin-I, and lipid profiles) declined by the extract (300 mg kg−1 body weight). Moreover, at a similar dose, it reduced the high circulatory biomarkers (alanine aminotransferase, alkaline phosphatase, aspartate aminotransferase, and total bilirubin) levels of hepatocyte injury. This study recommends promising pharmacological values of Bruguiera gymnorhiza ME.

Molecular docking Toxicological profile, Antioxidant and Antidiabetic Potential of Hydroalcoholic Extract of Leaves of Euphorbia indica (Lam.) from Saudi Arabia

Molecular docking Toxicological profile, Antioxidant and Antidiabetic Potential of Hydroalcoholic Extract of Leaves of Euphorbia indica (Lam.) from Saudi Arabia

Synergistic Assessment of Supplementation of Ascorbic Acid and Massularia acuminata Extracts on Serum Electrolyte and Lipid Profile Indices of Dyslipidemia in Adult Wistar Rats Exposed to Aluminum Chloride Toxicity

Background: Aluminum chloride (AlCl3) toxicity is a growing concern due to its prevalence usage in various industrial and environmental settings. Prolonged exposure to aluminum chloride can lead to oxidative stress, electrolyte imbalances, and alterations in lipid metabolism. This study aimed to investigate the effects of supplementation of ascorbic acid and Massularia acuminata extracts on electrolyte function and lipid profile parameters in adult Wistar rats exposed to aluminum chloride toxicity. Methodology: The stem barks of Massularia acuminata were air-dried at room temperature for thirty (30) days and were mechanically ground into fine powder by using an electrical mill. The powder was kept in air-tight container until use. Six hundred (600 g) of the dried powders was dissolved in 4 litres of 80 % (v/v) of methanol, ethanol and butanol respectively for 72 h with occasional agitation using sterile rod. The resulting mixture obtained was then filtered using pieces of white cotton gauze and concentrated in a rotary evaporator following by dryness under vacuo at 40 0C in a rotary evaporator. The extracts that were obtained were then concentrated to dryness ‘in vacuo’ to yield the respective solvent extracts For the animal study, then, fifty Adult Wistar rats was randomly divided into several groups, including a control group, an aluminum chloride-exposed group, and treatment groups supplemented with ascorbic acid, Massularia acuminata extracts, and combination of both in varied doses. The dosage of supplementation was determined based on preliminary experiments and relevant literature. The rats in the aluminum chloride-exposed group and the treatment groups received oral administration of aluminum chloride at a specific dose of (34 mg/kg) for a defined duration to induce toxicity. The control group received a placebo. The treatment groups received supplementation of Ascorbic Acid and Massularia acuminata extracts received daily oral supplementation of ascorbic acid, Massularia acuminata extracts, or a combination of both, while the control and aluminum chloride-exposed groups received a placebo. Results: The result showed that there was a significant (p < 0.05) decrease in the lipid profile of Total Cholesterol (TC), Very Low-Density Lipoprotein (VLDL) and a non-significant decrease in High Density Lipoprotein (HDL) treated with vitamin C while a significant decrease in the level of HDL, VLDL, LDL and cholesterol were observed from the groups treated with the various plant extracts. Conclusion: From the study, it can therefore be concluded that ethanol leaf extract of Massularia acuminata has protective effect against aluminium chloride induced hepatotoxicity, oxidative stress and alterations in lipid and electrolyte profile in Wistar rats.