Least Significant Difference Research Articles

Variations in olfactory function among bipolar disorder patients with different episodes and subtypes.

Most studies on olfactory function in individuals with bipolar disorder (BD) have not distinguished between the different subtypes or between the acute phase (mania or depression) and euthymic state. In this study, we compared olfactory function among BD patients with different subtypes and episodes to explore the potential use of olfactory function as a biomarker for the early identification of BD. The study sample consisted of 117 BD patients who were hospitalized between April 2019 and June 2019, and 47 healthy volunteers as controls. The BD patients were divided into a bipolar I disorder (BD I) (n = 86) and bipolar II disorder (BD II) group (n = 31) according to the different subtypes, and divided into depressive BD (n = 36), manic BD (n = 44), or euthymic BD (n = 37) groups according to the types of episodes they experienced. We assessed olfactory sensitivity (OS) and olfactory identification (OI) via the Sniffin' Sticks test and used the Hamilton Depression Rating Scale (HAMD) and Young Manic Rating Scale (YMRS) to evaluate BD characteristics among all subjects. Compared with controls, the participants with BD showed decreased OS and OI. We found statistically significant differences in OS and OI between the BD I group and controls, as well as differences in OS between the BD I and BD II group. Least-significant difference multiple comparisons revealed statistically significant differences in OS between the depressive BD group, manic BD group and controls and also between the manic BD and euthymic BD group. OI was positively correlated with the YMRS score in the BD I group and OS was negatively correlated with the HAMD score in the BD II group. This may be the first study to compare olfactory function in patients with BD I vs. BD II via pairwise comparisons. Our findings suggest that OS may have potential as a biomarker for distinguishing the different subtypes of BD and as a state-related biomarker for differentiating the acute phase from the euthymic state of BD. However, further prospective research is warranted.

Effect of organic, mineral and bio-fertilizer and their interaction on growth, and some quality characters of potato Solanum tuberosum L. cv. (Burren)

The experiment was conducted in the greenhouse of the Department of Horticulture and Garden Engineering - College of Agricultural Engineering Sciences - University of Baghdad in the spring season 2020-2021 within randomized complete block (RCBD) to study the effect of the foliar application with (Humic), biofertilizer (Biohealth) and balanced mineral fertilizer (NPK) 20:20:20 and the interaction among them on growth and yield of potatoes (Burren) cultivar, After completing the laboratory and field study indicators, the averages were compared to calculate the least significant difference (L.S.D) at 5% significance level. The results were as follows: The interaction between humistar and NPK was significantly superior in leaf chlorophyll content (0.916 mg.gm-1), while the number of stems per plant was not significantly different between NPK treatment and biofertilizer and triple interaction (3.33 stem. plant-1), While there were significant differences among the rest of the experimental treatments, while the treatment of biofertilizers was significantly superior in the stem diameter which gave (0.833 mm). The root length characters was superior in treatment of interaction between the biofertilizer and the NPK with significant difference compared to other treatments (37.00 cm), while the biofertilizer treatment significantly increased the weight of the roots by giving (91.00 gm). The percentage of soluble solids in tubers was highest in the treatment of triple interaction (organic, mineral and biological) with significant difference compared to the rest of the treatments (17.10%). The treatment of NPK and the interaction with the organic acid were significantly superior in leaf nitrogen and nitrate content by giving (1.85% and 125.67 ppm), whereas The leaf content of potassium showed that the interaction between organic acid and NPK and the triple interaction treatment significant differences compared to the rest of the experimental treatments which gave (1.78%).

Psychosocial and behavioral correlates of self-efficacy in treatment adherence in older patients with comorbid hypertension and type 2 diabetes

BackgroundAdhering to clinical prescriptions is known to protect against the effects of uncontrolled hypertension and of acute and chronic cardiovascular diseases, including diabetes. Contextually, positive associations between self-care behaviors and psychological constructs, such as self-efficacy, are widely acknowledged in the literature. However, still little is known about the psychological factors underlying the patient’s self-efficacy. This study aimed to investigate the psychosocial and behavioral correlates of self-efficacy related to treatment adherence in older patients with comorbid hypertension and type 2 diabetes mellitus.Participants and procedureItalian and Polish patients (≥ 65 years; N = 180) consecutively responded to self-report questionnaires measuring psycho-social (i.e., beliefs about medicines, perceived physician’s communication effectiveness, medication-specific social sup-port, self-efficacy) and behavioral factors (i.e., pharmacological adherence, medications refill adherence, intentional non-adherence) related to treatment adherence. Between-group comparisons and regression analyses were performed.ResultsFisher’s least significant difference (LSD) test showed significant differences between the Italian and Polish groups in all questionnaires (p < .01) with the Italian patients reporting more satisfactory scores. Younger age (β = .08, p = .045), female gender (β = 1.03, p = .042), higher medication refills adherence (β = –.07, p = .024), lower intentional non-adherence (β = –.03, p = .009), positive beliefs about medications (β = .13, p < .001), better quality of communication with the physician (β = .09, p < .001), and stronger perceived medication-specific social support (β = .06, p = .001) were significantly associat-ed with self-efficacy related to treatment adherence.ConclusionsFuture research and interventions should leverage psychosocial and behavioral factors to address self-efficacy contributing to enhancing adherence to clinical prescriptions.

PENGARUH PEMBERIAN ETHREL TERHADAP PEMBENTUKAN BUNGA DAN BUAH TANAMAN TOMAT (Lycopersicum pyriforme )

Background: Tomato is a vegetable plant that is popular with the public considering the various benefits of the tomato itself, so the demand for tomatoes is increasing. To obtain good results, especially in the process of growth and production of tomato plants, it is necessary to provide growth stimulants that are able to work in stimulating growth. tomato plants and stimulates the formation of flowers and fruit on tomato plants, one of which is ZPT Ethrel. Methods: This study used a completely randomized design (CRD) with 3 repetitions and 4 treatments; R0 = without ethrel ; R1 = 2 cc/liter ethrel; R2 = 4 cc/liter ethrel; R3 = 6 cc/liter ethrel, so the total test unit is 12 units. This research will be analyzed using the F test if the calculation F_(count)>f_table at a confidence level of 5% or 1% will be followed by the Least Significant Difference (LSD) test. Results: The results showed that the administration of ethrel affected the productivity of flower and fruit formation of tomato plants. The best flower and fruit formation was given 6 cc/liter ethrel, then 4 cc/liter ethrel, 2 cc/liter ethrel, and the lowest was without ethrel. Conclusion: Giving ethrel affects the formation of flowers and fruit of tomato plants (Lycopersicum pyriforme). The best flower and fruit formation was given R3 (6 cc/liter ethrel), then R2 (4 cc/liter ethrel), R1 (2 cc/liter ethrel), and the lowest was on R0 (without ethrel administration).

Improvement of Falcataria moluccana Root Growth by Giving Empty Palm Oil Fruit Bunches (EFB) Biochar in Growing Media

Sengon (Falcataria moluccana) is a fast-growing species widely planted in Industrial Plantation Forests (IPF/HTI) and has high economic value. The provision of planting material must be well prepared and planned to get the appropriate nursery planting media composition. The Empty Palm Oil Bunches (EFB) biochar application improves soil's chemical and physical properties to provide nutrients for plants. This study aims to determine the effect of the application of EFB biochar on the growth of sengon roots. The experimental design was completely randomized (CRD) by applying four treatment levels and 20 repetitions. The treatments were (A) 100% soil (control), (B) 5% EFB biochar, (C) 10% EFB biochar, and (D) 15% EFB biochar. The data were then analyzed by analysis of variance to see the effect of the treatment on the observed root growth parameters, followed by the 5% Least Significant Difference (LSD) further test to determine differences between treatments. The parameters observed were root length, root volume, root wet weight, and root dry weight. The result showed that the application of EFB biochar increased the growth of sengon roots compared to the control. The greater the dose of the addition of EFB biochar given to the growing media, the more it showed a positive response in improving the growth of sengon roots. In this study, the 15% dose showed the best results compared to other treatments.

Investigating the link between board independence and dividend distributions in South Africa

Purpose: As shareholder-elected monitors, independent non-executive directors (INEDs) should ensure that managers do not retain earnings to promote their own interests. The relationship between board independence and dividend distributions was hence investigated for selected companies listed on the Johannesburg Stock Exchange (JSE). The country offers a well-developed corporate governance framework to listed companies.Design/methodology/approach: Data on the considered companies’ dividend payout ratios (DPRs), board independence and six control variables were obtained from Bloomberg for the period 2007–2021. The significance of the observed trends in these variables was considered by conducting analysis of variance (ANOVA) and Fisher’s least significant difference (LSD) tests. The hypothesised relationship was assessed using a mixed-model regression.Findings/results: The results are in line with prior research showing that dividends are often omitted or reduced during and after crisis periods, that is, the global financial crisis (2008/2009) and the coronavirus disease 2019 (COVID-19) pandemic (2020/2021). A negative but statistically insignificant relationship was reported between DPR and board independence.Practical implications: Although board independence was not significantly related to dividend distributions for the sampled companies, INEDs still perform an important monitoring role. Shareholders are thus encouraged to play a more active role in the election of these directors.Originality/value: This study extends and refines previous research in South Africa and reveals new insights regarding board independence and dividend distributions during three King regimes and distribution-related regulatory changes.

Evaluating the Effects of Low-level Laser Therapy and Platelet-rich Plasma on Bone Calcium Content and Mechanical Properties at Tooth Extraction Sites: An Animal Study

Background: Both low-level laser therapy (LLLT) and platelet-rich plasma (PRP) are demonstrated to promote the repair of mandibular defects. Objectives: This study investigated the mechanical properties and calcium content at the tooth extraction site in a rat model exposed to LLLT (λ = 808 nm) with or without PRP. Methods: In this experimental rat model study, the left first molar maxillary teeth were extracted in twenty male rats. Then, the animals were randomly divided into four groups. Group one: after extraction, the extraction sockets were treated with 0.9 W gallium-aluminum-arsenide (GaAlAs) diode laser irradiation for five minutes every 72 hours for the next 12 days (4 times overall); group two: PRP was placed in the extraction sockets; group three: a combination of both treatments (LLLT+PRP) was applied; group four: the extraction sockets remained untreated (the control group). All rats were sacrificed 30 days post-operative. All bone blocks of the extracted socket were prepared for mechanical strength and calcium content analyses. Mann-Whitney test, one-way ANOVA test, and post hoc Fisher’s least significant difference (LSD) were used to analyze the data. A P-value less than 0.05 was considered significant. All analyses were performed by SPSS 16.0. The graph is illustrated in the graph pad 5. Results: The compressive strength in the laser group was significantly higher than in the control and PRP-treated groups (P = 0.0001 and 0.00044, respectively). Compared to the control and PRP groups, the effects of a combination of PRP and LLLT mechanical strength were statistically similar. Calcium content was influenced by none of the treatments. Conclusions: The mechanical strength of the bone blocks was significantly stronger in the LLLT group than in the other groups. Platelet-rich plasma alone or combined with LLLT demonstrated a synergistic impact on neither mechanical strength nor calcium content.

Pre-to-post Diwali air quality assessment and particulate matter characterization of a western coastal place in India.

Diwali has become an occasion of air and noise pollution, and the release of particulate matter and toxic gases has chronic and acute effects on people and their environment. Thus, an air quality assessment study was done by CSIR-CSMCRI covering the pre-to-post Diwali 2021 period (5days) in the three locations (traffic, residential, and control) of Bhavnagar. The average 24-h concentration of PM10 (380µg/m3), PM2.5 (182.2µg/m3), and SPM (403µg/m3) was significantly higher during Diwali, exceeding the National Ambient Air Quality Standards (NAAQS). The concentrations of SO2 and NO2 were 121.8µg/m3 and 102.1µg/m3. Metals like Zn, Al, Pb, and Mn were found in higher concentrations during the study. The air quality index (AQI) was maximum on Diwali, resembling very poor air quality. More elements and oxides were detected in PM2.5 (S, Al, Mg, Ba, and Zn and their oxides) than in PM10 (Fe and S) through WDXRF. Water-soluble anions like SO42-, Cl-, and NO3- were observed during the study, with a higher SO42- (64%) on Diwali. The PM10 morphology and mapping of elements were done using SEM-EDX. Emerging contaminants, specifically phthalate groups, were detected through GCMS. The enrichment factor (EF) showed Zn and Pb originating from anthropogenic activities. The air quality data was validated using a variance test, least significance difference (LSD), correlation, and principal component analysis (PCA). This paper is the first to highlight the air quality assessment during Diwali for a western coastal place in India. It is time to implement regulations on burning firecrackers for pollution reduction, aiming to achieve a sustainable atmosphere.

Evaluation of driver demand for in-vehicle information: An integrated method combining clustering and multivariate ordered probit model

Introduction: The proper execution of driving tasks requires information support. While new technologies have increased the convenience of information access, they have also increased the risk of driver distraction and information overload. Meeting drivers’ demands and providing them with adequate information are crucial to driving safety. Methods: Based on a sample of 1,060 questionnaires, research on driving information demands is conducted from the perspective of drivers. A principal component analysis and the entropy method are integrated to quantify the driving information demands and preferences of drivers. The K-means classification algorithm is selected to classify the different types of driving information demands, including dynamic traffic information demands (DTIDs), static traffic information demands (STIDs), automotive driving status information demands (ATIDs), and total driving information demands (TDIDs). Fisher's least significant difference (LSD) is used to compare the differences in the numbers of self-reported crashes among different driving information demand levels. A multivariate ordered probit model is established to explore the potential factors that influence the different types of driving information demand levels. Results: The DTID is the driver's most in-demand information type, and accordingly, gender, driving experience, average driving mileage, driving skills, and driving style significantly affect the driving information demand levels. Moreover, the number of self-reported crashes decreased as the DTID, ATID, and TDID levels decreased. Conclusion: Driving information demands are affected by a variety of factors. This study also provides evidence that drivers who have higher driving information demands are more likely to drive more carefully and safely than their counterparts who do not exhibit high driving information demands. Practical implications: The results are indicative of the driver-oriented design of in-vehicle information systems and the development of dynamic information services as a way to avoid negative impacts on driving.

Exploring the Differences in Work Value Among Generations in the UAE

This study aims to measure the work values of Generation X, Generation Y, and Generation Z and address the generational differences based on their work values. An appropriate sample was used where respondents were employees working, across the United Arab Emirates, in different industries in the private and public sectors. The data were collected from 130 employees based on a 45-item work value inventory (WVI). Fisher's least significant difference (LSD) statistical test was used to answer the research question based on multivariate tests across all three generations. Findings have suggested that Generation X placed more emphasis on work values as compared to Generation Z and Generation Y and were differentiated based on surroundings, altruism, and way of life work values.

Improving the creative thinking and collaborative skills of prospective biology teachers using the EMKONTAN learning model in environmental science courses

The implementation of the Environmental Science learning process so far has shown a lack of encouragement to improve students' 4C skills. The EMKONTAN learning model is one of the models that is believed to improve students' creativity, collaboration skills, and environmental literacy. This study aims to analyze the effectiveness of the EMKONTAN learning model in improving critical thinking and collaborative skills for prospective biology teacher students. EMKONTAN is a student-centered learning model (students’ active learning), oriented to creative learning, problem-based learning, collaborative learning, and provides opportunities for students to improve environmental learning. This research was conducted in the event semester of the 2020/2021 academic year at IKIP Budi Utomo Malang and FTTE Universitas Muhammadiyah Malang-Indonesia, involving 150 second-semester students through total sampling. Data was obtained through observation, questionnaires, and tests. The research used a pretest-posttest non-equivalent control group design. Data collection in this study was carried out using Google Forms, Google Classroom, Google Meet, and WhatsApp. Data analysis using statistical product and service solutions (SPSS) version 23 software. The analysis of covariance (ANCOVA) analysis results showed that EMKONTAN, PBL affected students' creativity and collaboration skills in biology teacher candidate students with a value of p<0.005. The least significant difference (LSD) result was significantly different in improving students' creativities thinking, and collaboration skills. The EMKONTAN class gained the highest post-test score. Therefore, EMKONTAN could be applicable to improve students' creative thinking and collaborative skills outcomes in environmental learning.

Discoloration of stretched colored elastomeric modules : An in vivo study.

As the demands on esthetic orthodontic devices continue to increase, discoloration of colored elastomeric modules is still a concern for patients and orthodontics. Thus, we performed an in vivo study evaluating discoloration of stretched colored elastomeric modules (i.e., white, gray, and pink). In all, 90elastomeric modules of three different colors (white, gray and pink; 30elastics from each color) were placed around brackets. The elastomeric modules were distributed and arranged randomly in the patients' four dental quadrants, and were removed after 1, 2, and 4weeks. Color measurements were made before and after insertion into each patient's mouth. Digital images were taken with the camera of amobile phone (iPhoneX, Cupertino, CA, USA), and the variations in color were characterized using the Commission Internationale del'Eclairage (CIE) color space system (L*a*b*) offered by Photoshop (Adobe, San Jose, CA, USA). Analysis of variance (ANOVA) and least significant difference (LSD) tests were used for statistical analyses. The white elastomeric modules were most prone to discoloration, followed by the pink and then finally by the gray colored modules. The amount of discoloration resulting from arandom diet practiced by the patients increased gradually (but was not significant) for all elastomeric modules studied and reach apeak after 2weeks for the white and gray elastomeric modules, while discoloration of the pink elastomeric modules plateaued after 1week. Colored elastomeric modules (e.g., white, gray, and pink) discolor to variable degrees over time. Thus, patients should be advised that the consumption of food and drinks may result in discoloration of the elastomeric modules.

The potential of the discovery learning model integrated the reading, questioning, and answering model on cross-cultural high school students’ problem-solving skills

This study aims to test the discovery model integrated with the reading, questioning, and answering (RQA) model. This research is a quasi-study which involves the second semester XI grade students of state senior high school (SMA) Negeri in Situbondo as subjects. The number of students who studied was 160 people from four different classes in the 2018/2019 academic year. Sampling is done by class equality test. Each class is taught with a different model, for each class is grouped again based on the culture of residence of students, namely coastal students, urban and mountain students. Researchers used Ancova to test the research hypothesis followed by the least significant difference (LSD) test. The results showed that: i) RQA’s integrated discovery learning model is able to improve students’ problem-solving skills compared to the original model; ii) Culture has a significant role in shaping students’ problem-solving skills, with the use of the same model given to students who have a background a different background will get different results; and iii) Learning in school and in the environment is an interrelated factor in shaping students’ thinking patterns and problem-solving skills. Discovery learning model integrated learning RQA model is a combination of effective models to be applied to students who are trained with challenges where nature teaches them to think and act appropriately and quickly.

Relationship between Potential Trace Elements Contamination in Sediment and Macrofauna in the Upper Gulf of Thailand.

The relationship between heavy metal contamination in sediment and macrofauna in the upper Gulf of Thailand is presented as an indication of the environmental quality of coastal waters. This study aims to monitor five heavy metal elements (As, Cd, Mn, Ni, and Pb) between sediment and the sandworm (Perinereis quatrefagesi (P. quatrefagesi)). The geoaccumulation index (I geo), enrichment factor (EF), and contamination factor (CF), including the pollution load index (PLI), were used for statistical analyses by one-way analysis of variance (ANOVA), and differences in the data were compared using the least significant difference (LSD) test. The sediment heavy metal concentrations were found to decrease (Al > Mn > Pb > Ni > Cd > As), and the quantity of heavy metal contamination in the sediment was not over the emergency point defined by the Pollution Control Department in Thailand. The Mn at the SH and SP points has I geo > 0 but <1 and is considered not polluted to moderately polluted. The EF overall is <2 and is deficient in mineral enrichment. The CF of the Samut Sakhon (SH) and Samut Prakan (SP) sites had high Al and Mn values, indicating a low pollution level, but the PLI had an all-site average of 0.0215 ± 0.0252, which is <1. This indicates that the present areas are close to ideal and not polluted. To measure heavy metals in macrofauna, P. quatrefagesi was assessed, and concentrations were found to decrease (Mn > Ni > Cd > Pb > As). The Mn between sediment and the sandworm was directly correlated (r = 0.976; p < 0.01). The sandworm performs as a bioindicator for the quality of coastal sediment, specifically with Mn; thus, the result present at a baseline level may grow in the future if there is no control measure for defensive measures.

Changes in Serum Concentrations of Vascular Endothelial Growth Factors-A and B after Intravitreal Injection of Ranibizumab and Conbercept for Retinopathy of Prematurity

Introduction: The aim of this study was to compare the changes in serum concentrations of vascular endothelial growth factor (VEGF)-A and B after intravitreal injection of ranibizumab (IVR) or conbercept (IVC) for retinopathy of prematurity (ROP). Methods: In this prospective study, infants with type 1 ROP in both eyes were recruited in our hospital from September 2021 to February 2022, randomly assigned to the ranibizumab and conbercept groups and administered IVR or IVC (0.25 mg/0.025 mL). Blood samples were collected before the operation and 1 and 4 weeks after the operation to measure the concentrations of serum VEGF-A and B. Results: A total of 20 ROP infants were randomly assigned to the ranibizumab (n = 10) and conbercept groups (n = 10). In the ranibizumab group, the serum VEGF-A concentrations before operation and 1 and 4 weeks after the operation were 73.55 ± 40.78, 11.47 ± 7.00, and 75.36 ± 30.87 pg/mL, respectively (p < 0.01). Least Significant Difference (LSD) pairwise comparison did not show any significant difference in the groups between 4 weeks postoperatively and preoperatively (p > 0.05). In the conbercept group, the serum VEGF-A concentrations before operation and 1 and 4 weeks after the operation were 86.69 ± 55.06, 14.68 ± 10.11, and 43.55 ± 57.92 pg/mL, respectively (p < 0.01). LSD comparison showed significant differences between 1 week and 4 weeks postoperatively and preoperatively (p < 0.05), but no significant differences were observed between 1 and 4 weeks postoperatively (p > 0.05). Regarding serum VEGF-B concentrations before the operation and 1 and 4 weeks after the operation, no significant differences were detected in the ranibizumab group (p > 0.05), but significant differences were observed in the conbercept group (7.26 ± 2.34, 3.09 ± 2.41, and 4.55 ± 3.37 ng/mL, respectively; p < 0.01). LSD showed significant differences between 1 or 4 weeks postoperatively and preoperatively (p < 0.05), but no significant difference was detected between 1 and 4 weeks postoperatively (p > 0.05). Conclusions: Serum VEGF-A levels in infants with ROP were suppressed after IVR or IVC but returned to preoperative levels at 4 weeks after IVR and remained lower than the preoperative levels at 4 weeks after IVC. Serum VEGF-B was not affected by IVR but was suppressed by IVC for 4 weeks.

Effects of six weeks of sub-plateau cold environment training on physical functioning and athletic ability in elite parallel giant slalom snowboard athletes.

Hypoxic and cold environments have been shown to improve the function and performance of athletes. However, it is unclear whether the combination of subalpine conditions and cold temperatures may have a greater effect. The present study aims to investigate the effects of 6 weeks of training in a sub-plateau cold environment on the physical function and athletic ability of elite parallel giant slalom snowboard athletes. Nine elite athletes (four males and five females) participated in the study. The athletes underwent 6 weeks of high intensity ski-specific technical training (150 min/session, six times/week) and medium-intensity physical training (120 min/session, six times/week) prior to the Beijing 2021 Winter Olympic Games test competition. The physiological and biochemical parameters were collected from elbow venous blood samples after each 2-week session to assess the athletes' physical functional status. The athletes' athletic ability was evaluated by measuring their maximal oxygen uptake, Wingate 30 s anaerobic capacity, 30 m sprint run, and race performance. Measurements were taken before and after participating in the training program for six weeks. The repeated measure ANOVA was used to test the overall differences of blood physiological and biochemical indicators. For indicators with significant time main effects, post-hoc tests were conducted using the least significant difference (LSD) method. The paired-samples t-test was used to analyze changes in athletic ability indicators before and after training. (1) There was a significant overall time effect for red blood cells (RBC) and white blood cells (WBC) in males; there was also a significant effect on the percentage of lymphocytes (LY%), serum testosterone (T), and testosterone to cortisol ratio (T/C) in females (p<0.001-0.015, ). In addition, a significant time effect was also found for blood urea(BU), serum creatine kinase (CK), and serum cortisol levels in both male and female athletes (p=0.001-0.029, ). (2) BU and CK levels in males and LY% in females were all significantly higher at week 6 (p=0.001-0.038), while WBC in males was significantly lower (p=0.030). T and T/C were significantly lower in females at week 2 compared to pre-training (p=0.007, 0.008, respectively), while cortisol (C) was significantly higher in males and females at weeks 2 and 4 (p (male)=0.015, 0.004, respectively; p (female)=0.024, 0.030, respectively). (3) There was a noticeable increase in relative maximal oxygen uptake, Wingate 30 s relative average anaerobic power, 30 m sprint run performance, and race performance in comparison to the pre-training measurements (p<0.001-0.027). Six weeks of sub-plateau cold environment training may improve physical functioning and promote aerobic and anaerobic capacity for parallel giant slalom snowboard athletes. Furthermore, male athletes had a greater improvement of physical functioning and athletic ability when trained in sub-plateau cold environments.

Performance of food–feed maize and cowpea cultivars under monoculture and intercropping systems: Grain yield, fodder biomass, and nutritive value

Livestock feeding in Burkina Faso is characterized by a recurrent deficit in both the quality and the quantity of fodder during the dry season, which affects animal performance. To overcome this, quality fodder/forage production is an alternative. Therefore, this study evaluated food- and feed-improved cultivars of maize and cowpea in intercropping trials using the “mother and baby trials” approach with crop–livestock farmers. The mother trial comprised a randomized block design with eight treatments and four replicates: two cowpea (KVx745-11P and Tiligré) and two maize cultivars (Barka and Espoir), and grown under two cropping systems (monoculture and intercropping). Baby trials were established on-farm and involved 30 farmers during two seasons (2019 and 2020) in four villages in the South Sudan zone of Burkina Faso. Data were collected on (1) weed density and biomass, (2) grain yield and fodder biomass, (3) intercropping efficiency, and (4) fodder nutritive value. Data were analyzed using ANOVA and the least significant difference (LSD) means separation at a 5% threshold. The results revealed that maize and cowpea intercropping significantly reduced weed biomass (p ≤ 0.05). In monoculture, the maize cultivar Barka produced a greater grain yield (4980 kg/ha) and fodder biomass [6259 kg dry matter (DM)/ha] than the cultivar Espoir, which produced a grain yield of 2581 kg/ha and fodder biomass of 4952 kg DM/ha. The cowpea cultivars, KVx745-11P and Tiligré, were similar (p ≥ 0.05) in terms of fodder biomass (2435–2820 kg DM/ha) and grain yield (1152–1163 kg/ha). For the intercropping system, land equivalent ratios for fodder biomass (1.18:1.41) and grain yield (1.02:1.44) were greater than 1; intercropping also had better productivity system indexes than the monoculture cropping system. The crude protein concentration of fodder was greater for Barka maize (9.5%–9.8%) than for Espoir maize (8.5%–8.7%). The crude protein concentration was greater for cowpea KVx745-11P (19%–21.8%) than for cowpea Tiligré (15%–17%). Intercropping both Barka maize and cowpea KVx745-11P was the most productive cropping system for maximizing grain and fodder production for crop–livestock farmers in the South Sudan zone of Burkina Faso.

Effects of Dietary Flaxseed Oil and Ascorbic Acid on the Reproductive Performance of South African Indigenous Sheep

The current study aimed to evaluate the effects of the dietary inclusion of flaxseed oil and ascorbic acid on the reproductive performance of South African indigenous sheep (Ovis aries). Twenty-two matured South African indigenous rams (eight BaPedi, nine Zulu, and five Namaqua Afrikaner; age of 6 years and body weight of 64.4 ± 1.6 kg) were randomly assigned into five treatment groups (NC (standard diet), PC (basal diet), FO (5% flaxseed oil), AA (4% ascorbic acid), and FO + AA (5% flaxseed oil + 4% ascorbic acid)). Semen samples were collected during the natural breeding season using an artificial vagina. Semen samples were evaluated for macroscopic (semen volume, pH, and sperm concentration) and microscopic (morphology, malondialdehyde, membrane permeability, and sperm cell motility parameters) characteristics. Eighty-one South African indigenous ewes were synchronized using controlled intravaginal drug devices impregnated with 0.3 g progesterone for 10 days and then injected with 300 IU of Pregnant Mare Serum Gonadotropin. These ewes were then hand-mated. The data were subjected to the General Linear Model (GLM) in Minitub® 2017. A cross-tabulated Chi-Squared (χ2) test was used to track the frequencies of the conception rate. Treatment means were separated using Student’s Least Significant Difference (LSD) and considered significantly different when the p-value was less than 0.05. No considerable breed effect was observed for semen volume, semen pH, sperm concentration, intact membrane, and non-intact membrane. FO + AA led to higher semen volume (1.05 ± 0.06 mL), intact sperm membranes (88.83 ± 1.27%), and low malondialdehyde levels (0.37 ± 0.04 nmol/mL) in comparison to other treatment groups. Total sperm motility was higher in FO + AA (95.81 ± 0.80%) compared to FO, AA, PC, and NC. The conception rate was higher in FO (94%), AA (94%), and FO + AA (100%) when compared to NC (71%) and PC (79%). The testosterone concentration did not differ among PC, FO, AA, and FO + AA. It was concluded that the inclusion of 5% flaxseed oil (FO and FO + AA) improves both the semen quality and the conception rate of South African indigenous sheep.

Yield-scaled and area-scaled greenhouse gas emissions from common soil fertility management practices under smallholder maize fields in Kenya

Agricultural land-use activities are the leading sources of GHG (greenhouse gas) emissions in Kenya. However, few studies have examined GHG emissions resulting from different soil fertility management practices in small-scale African agricultural systems. The objective of the study was to quantify on-farm GHG emissions under different maize cropping practices in Tharaka-Nithi County, Kenya. The static chamber technique following a randomized rotational commencement pattern between farms and plots during GHG sampling events was used. Annual grain yield was determined from net plots and reported at 12.5 % moisture content. During analysis, analysis of variance (ANOVA) followed by LSD (Least Significant Difference) tests were used to assess treatment effects on grain yield, nitrogen use efficiency, area-scaled, and yield-scaled GHG emissions using GenStat and R procedures (‘agricolae’ package). The results showed that maize yield was not statistically different between fertilizer and fertilizer+manure treatments. The highest GNU (Grain nitrogen uptake) values were recorded in the fertilizer treatment, followed by fertilizer + manure, sole manure, and the control, which recorded the least GNU. The greatest GHG sinks were observed in sole manure, followed by fertilizer+manure, fertilizer, while the control treatment recorded the least GHG sinks. Additionally, the highest grain yields were obtained in the fertilizer treatment, followed by fertilizer+manure, and sole manure, while the control recorded the least yield. The study concluded that organic manure integration contributed significantly to improved soil fertility and GHG sequestration benefits without compromising maize yields.

Avocado ( Persea americana Mill.) Seed Extract Cream 10% Inhibited The Increase MMP-12 Enzyme Levels And Elastosis In UVA Exposed Hairless Male Wistar Rats Skin

Background: Skin is the main target of environmental influences, especially chronic exposure to the suns UVA rays which will result in wrinkling, decreased skin elasticity, the appearance of loose skin, and a rough clinical appearance. In the dermal tissue, not only affects collagen fibers but also degradation of elastin fibers occurs through an increase in the MMP-12 enzyme levels called photoaging and is characterized by the accumulation of dystrophic elastin fibers throughout the dermal tissue in a process called Elastosis. Avocado seed (Persea americana Mill.) is a part of the fruit that contains high concentrations of flavonoids, phenols, and tannins, it has the potential as a powerful antioxidant. The aim of the present study is to evaluate the effectiveness of avocado (Persea americana Mill.) seed extract cream 10% inhibiting the increase in MMP-12 enzyme levels and elastosis in hairless male Wistar rats (Rattus norvegicus) which were exposed to ultraviolet A rays. Methods: A Randomized post-test-only control group design was conducted among 30 male Wistar rats for 10 consecutive days. They were divided into 3 groups as follows: group (K) as the negative control without any intervention, group (P1) with UVA rays exposure, and group (P2) with application of avocado (Persea americana Mill.) seed extraxt cream 10% and UVA rays exposure. The UVA dosage used is 5J/cm2 twice a day among P1 and P2 groups. Avocado (Persea americana Mill.) seed extract 10% was applied to the hairless Wistar rats 4 hours after the exposure of UVA rays. MMP-12 was calculated with Rat MMP-12 ELISA Test. Elastin fiber density was calculated by Imageraster software. Statistical analysis was carried out using SPSS version 25 in measure means, normality test, homogeneity test, and comparative test. Results: The results showed that the average MMP-12 enzyme level in the 10% avocado seed cream (P2) group 18.138 ± 0.168% was significantly different from the (K) group 1.073 ± 0.1553% as the negative control and the (P1) group 33.454 ± 1.068%. The mean density of elastin fiber in the cream of avocado seed extract group (P2) 12.42 ± 3.672% was significantly different from the group (K) 20.08 ± 4.994% and group (P1) 26.30 ± 6.822%. Comparative analysis using the Kruskal Wallis test for MMP-12 and the One Way Annova test for elastin fibers showed a p-value of < 0.05, thus indicating a significant difference between groups after treatment. The Least Significance Difference (LSD) test showed that there was a significant difference between the cream of avocado seed extract (P2) group and the other groups (p<0.05). Conclusions: The administration of avocado seeds (Persea americana Mill.) extract cream 10% effectively inhibits the increase in MMP-12 enzyme levels and elastosis in hairless male Wistar rat skin which was exposed to ultraviolet A irradiation.