Aloe Vera Leaf Extract Research Articles

Anti-edema effect of Aloe vera leaf extract following traumatic brain injury: Role of pro-inflammatory cytokines.

Objective:Based on anti-inflammatory effects of Aloe vera, the effect of aqueous extract of this plant on brain edema and changes in some pro-inflammatory cytokines was investigated after traumatic brain injury (TBI).Materials and Methods:In this study, adult male Wistar rats were divided into 5 groups: Sham, TBI, vehicle (Veh), and low dose (LA) and high dose (HA) Aloe vera. The vehicle and aqueous extract of Aloe vera were injected intraperitoneally 30 min after induction of diffuse TBI by Marmarou’s method. Brain edema (brain water content), and transforming growth factor beta (TGF-β), tumor necrosis factor alpha (TNFα), interleukin 6 (IL-6) and IL-1β levels in serum and brain were measured 24 hr after TBI induction.Results:Increased brain edema by TBI was reduced by both LA and HA (p<0.01 and p<0.05, respectively). IL-6 increased in the brain of TBI group compared to sham, and which was inhibited by both Aloe vera doses compared to Veh (p<0.001). The differences in the IL-6 serum levels among Veh, LA and HA groups were not significant. Increases in serum and brain IL-1β levels were reduced only in the HA group (p<0.001). Although only in the brain, TNF-α level increased after trauma, but both LA and HA inhibited it in a dose-dependent manner (p<0.01 and p<0.05, respectively) . The amount of TGF-β in the brain was reduced by both doses of the extract (p<0.001). Conclusion:These results indicated that Aloe vera has a neuroprotective effect induced by reducing brain edema. The probable mechanism particularly for HA is decreasing levels of pro-inflammatory cytokines such as TGF-β, TNF-α, IL-6 and IL-1β.

NiO bio-composite materials: Photocatalytic, electrochemical and supercapacitor applications

Synthesis based on biomaterial technology has high potential in structural applications. Blending metal oxides with plant extract is an attractive alternative for preparing materials with superior material properties. Here, we report for the first time, the synthesis of NiO bio-composite materials by employing Aloe vera leaf extract. The work mainly highlights the synergistic influence of biomolecules of Aloe vera plant on the electrochemical and photocatalytic properties of NiO. The structure, morphology, band gap and bonding features of NiO bio-composites were characterized by XRD, Electron Microscopy, UV-DRS and FT-IR techniques, respectively. The average crystallite size of the composites was within 60 nm. FT-IR spectra confirmed the strong bonding interactions between biomolecules and NiO. A maximum band gap of 3.88 eV with a good thermal stability for the synthesized composites was observed. The addition of biomolecules to NiO resulted in the improvement of the reversibility of the electrode by reducing the anodic (Epa) and cathodic (Epc) peak potential difference in KOH electrolyte. Also, the composite exhibited greater stability due to recyclability and less resistance even after 1000 charge-discharge cycles, revealing a stable electrode material for supercapacitors applications. The photocatalytic experiments confirmed a maximum of 95% dye degradation efficiency by the composite for AR88 dye. It was also found that 50 % of dye degradation occurred within 74.61 min. The encouraging results project out the potential of the synthesized NiO-biocomposites to be a better supercapacitor and photocatalyst.

The Green Synthesis of Nanoparticle Zinc Oxide (ZnO) Using Aloe Vera Leaf Extract: Structural and Optical Characterization Reviews

The Green Synthesis of Nanoparticle Zinc Oxide (ZnO) Using Aloe Vera Leaf Extract: Structural and Optical Characterization Reviews

Haematology and Serum Biochemical Indices of Lambs Supplemented with Moringa oleifera, Jatropha curcas and Aloe vera Leaf Extract as Anti-Methanogenic Additives.

Medicinal plants have been found to be effective in a wide range of applications in ruminant animals. However, some plant extracts may be toxic to animals, depending on their seconday metabolite composition and dose, and therefore, animal trials are needed to validate their safety when used as anti-methanogenic additives. This study investigated the effect of three plant extracts used as anti-methanogenic dietary additives, on the haematology and serum biochemical parameters in sheep. Methanolic extracts of Moringa oleifera (MO), Jatropha curcas (JC) and Aloe vera (AV) were orally dosed as experimental treatments for 75 days to sheep, and their effect on the haematology and serum biochemical parameters of SA Mutton Merino (SAMM) lambs were compared with sheep on a control treatment without any additive treatment. Extracts of MO, JC and AV were extracted in 100% methanol, freeze-dried, and reconstituted in distilled water. A total of 40 lambs were ranked according to their body weight into a group of four and one sheep at a time was randomly allocated into four dietary treatments which include a control treatment, and treatment with either MO, JC or AV extract. Lambs were drenched twice daily with doses equivalent to 50 mg/kg dry matter intake (DMI) based on previous week feed consumption. Blood samples were collected via jugular vein puncture and analysed for haematology and serum biochemistry parameters, using standard procedures. The results of the haematological analysis showed that most haematological parameters were not affected by plant extract used as anti-methanogenic additives (p > 0.05), except for higher white blood cell (WBC) and lymphocytes counts recorded in control lambs and lambs in the AV treatment. All serum biochemical properties (except alkaline phosphatase) were not different (p > 0.05) between the control and lambs treated with plant extracts. Alkaline phosphatase was influenced by the plant extract (p < 0.05), with lambs receiving MO, JC and AV having lower alkaline phosphatase concentrations compared to lambs on the control diet without any additive. The result of the study showed that extracts of MO, JC and AV were not toxic to sheep when used as antimethanogenic additives at the recommended dose of 50 mg/kg dry matter feed which had proved previously to be effective in reducing enteric methane emission. Therefore, these plant extracts could be used safely as alternative dietary additives to reduce enteric methane emission and boost the productivity of SA Mutton Merino sheep.

Green synthesis of copper oxide nanoparticles using aloe vera extract

&#x0D; &#x0D; &#x0D; In material science, green method for synthesis of nanomaterials is feasible, cheaper and eco-friendly protocol. To accomplish this phenomenon, present study was aimed to synthesize Copper oxide nanoparticles using leaf extract of Aloevera with two different precursors CuCl2.2H2O (Cupric chloride) and CuSo4.5H2O (Cupric sulfate). The extraction of Aloevera is employed as reducing and stabilizing agent for this synthesis.Copper oxide Nanoparticles is effective use of biomedical application due to their antibacterial function. The synthesized Copper oxide nanoparticles were characterized by X-Ray Diffraction Spectroscopy (XRD), Energy Dispersive Spectroscopy (EDX), FourierTransform Infrared Spectroscopy (FT- IR) and Scanning Electron Microscope(SEM). XRD studies reveal the crystallographic nature of Copper oxide nanoparticles. Furthermore the Copper oxide nanoparticles have good Antibacterial activity against both gram negative (E.Coli, Klebsiella pneumonia) and gram positive (Bacillus cereus, Staphylococcus aureus)bacteria.&#x0D; &#x0D; &#x0D;

Enhanced bioactive compounds composition in Lavandula officinalis in-vitro plantlets using NaCl and Moringa oleifera, Aloe vera and Spirulina platensis extracts

Biotic and abiotic elicitors have been reported to promote growth or multiplication as well as alter bioactive compositions of different plant species. Lavandula officinalis is a perennial plant, which produces fragrant flowers that are utilized to prepare the lavender oil. Lavender oil is rich in beta-linalool, cineol, camphor and many other bioactive compounds, which together contributes to its pain relieving, antimicrobial and cicatrizing activities. Micropropagation is a good alternative to propagate lavender as it reduces the time required to achieve the production of essential oil and eliminates the exposures of the lavender plants to environmental pollutants, thus ensuring a better quality of the oil produced. In this study, we have investigated the effects of 3 biotic elicitors - Moringa oleifera leaf extract (MLE), Aloe vera leaf extract (ALE), Spirulina platensis aqueous extract (SAE) and an abiotic elicitor - Sodium chloride (NaCl) on the growth, multiplication and phytochemical compositions of Lavandula officinalis in-vitro plantlets. All tested biotic elicitors (MLE, ALE, SAE) negatively affected the plantlets fresh weight and shoots height. However, these elicitors were found to enhance the number of leaves per explant. On the other hand, the effect of abiotic elicitor (NaCl) were opposite to that of the biotic elicitors. In contrast to the negative growth effects, SAE and high ALE concentration (40 ml/L) were found to enhance number of shoots per explant. Interestingly, we found that all tested biotic and abiotic elicitors were able to increase the beta-linalool contents in the L. officinalis in-vitro plantlets. We observed that SAE is the best elicitor among the 4 tested elicitors because both 0.5 and 1.0 g/L SAE were able to increase linalool production and enhanced shoots proliferation.

Aloe-vera leaf extract as a green agent for the synthesis of CuO nanoparticles inactivating bacterial pathogens and dye

The present work describes the aloe-vera, as a green agent for the facile synthesis of copper oxide NPs and their deliberation for antibacterial and photocatalytic activities. The synthesis has been carried out at pH 11.5 and 70 °C with detailed characterization investigation through UV-spectroscopy, XRD (X-ray Diffraction), SEM (Scanning electron microscope), EDX (Energy-dispersive X-ray spectroscopy) and TEM (Transmission electron microscope) techniques. The study reveals the altered morphology of CuO NPs with a change in concentration of precursor. After characterization, CuO NPs with size 5–20 nm have been examined against pathogenic bacteria like Bacillus subtilis, Staphylococcus aureus, and Escherichia coli to calculate the zone of inhibition, MIC (minimum inhibitory concentration) and MBC (minimum bactericidal concentration). Photo-catalytic degradation has been performed in the presence of H2O2, which results in the degradation of MO (methyl orange) dye up to 96% in 24 min under exposure of UV light.

A Pilot Study on Aloe Vera Leaf Extract in Cream Base for the Clinical Improvement of Melasma: A Split-Face Trial

A Pilot Study on Aloe Vera Leaf Extract in Cream Base for the Clinical Improvement of Melasma: A Split-Face Trial

Effects of synthesized silver and chitosan nanoparticles using Nerium oleander and Aloe vera on antioxidant enzymes in Musca domestica

Green synthesis of silver and chitosan nanoparticles have been achieved using Nerium.oleander and Aloe vera leaf extract as reducing agents. The formation of nanoparticles was quantified by UV-vis spectroscopy of wave length from 200-700. TEM, XRD and FTIR were used for characterization. The present investigation was carried out to assess the activity of antioxidant enzymes, SOD, CAT, GPx and GST in third instar larvae of Musca domestica under the effect of methanol leaf extract, synthesized silver and chitosan nanoparticles and the LC50 was used for biochemical assay. The activity of antioxidant enzymes in M. domestica larvae were varied with the time where an increase in SOD activity was recorded after 24hrs in methanol extract and AgNPs treated larvae, then decreased again after 48hrs. In CsNPs treated larvae, SOD induced a continuously decreased activity at all times of investigation. CAT activity followed the same trend of SOD. After 48hrs of treatment the activity of GPs was significantly decreased in all treated groups, at 1% and 5% level of significance in larvae treated with chitosan Nerium and chitosan A. vera, respectively. After an increase in GST activity at 24hrs, a decrease was noticed after 48hrs. From the present study, the use of N. oleander and A. vera as methanol crude extracts to synthesize silver and chitosan nanoparticles could be a new approach for the control of house flies.

Green Fabrication of MgO Nanostructure for Morphology and Optical Studies

Green synthesis of multifunctional magnesium oxide nanoparticles (NPs) were prepared by low temperature solution combustion route employing Aloe vera leaves extract as a fuel. The Aloe vera leaf extracts acts as a fuel in the preparation of MgO nanoparticles. The obtained MgO NPs were further characterized by various techniques such as PXRD, SEM, TEM and UV-visible spectroscopic studies. The surface morphologies of the prepared samples were studied with the help of scanning electron microscopy (SEM). The PXRD pattern confirmed the cubic (halite) structure of the product. The particle size was calculated by means of PXRD profile and it was found to be in the range of 20-25 nm by using the Debye-Scherrer relation. SEM images indicated the formation of various shaped MgO NPs. The NPs exhibited prominent green emission due to the presence of intrinsic defect centers. Also, the results found that the optical band gap is 4.46 eV.

Synthesis of Zinc Oxide Nanoparticles by using Aloe Vera Leaf Extract as Pontential Anode Material in Lithium Ion Battery

Synthesis of nanoparticles by using plant have sparked interest among researchers due to environmentally safe, inexpensive and simple method to compare with chemical method. Use of plant in synthesis zinc oxide nanoparticles (ZnO NPs) that act as reducing and capping agent are more recommended, due to high production of product and rate of synthesis is faster than using microorganism. This study focus on the synthesis of ZnONPs by using leaf extract of aloe vera (Aloe bardenisis miller) with different concentration (30%, 40% and 50%) and various calcination temperature which are 500 ˚C, 700 ˚C and 900 ˚C for 4 hours. Fourier – transform infrared spectroscopy (FTIR), Thermogravimetric Analysis (TGA), scanning electron microscopy (SEM), X-ray Diffraction (XRD) and Brunauer-Emmet and Teller (BET) were used to characterize the prepared samples. FTIR spectra showed present wavenumber in between 400-500 cm-1 indicated the presence of Zn-O stretch. Powder XRD pattern confirmed the hexagonal wurtzite structure with average particles size from 24.19 nm to 67.69 nm for all concentration and temperature by using Scherer’s equation. For SEM analysis the images show irregular shape for concentrations 30% and 50% with size range from 500 nm to 900 nm while for concentration 40% cubic shape was observe with size range from 140 nm to 900 nm. All characterize show that formation of ZnO NPs depend on the concentration and calcination temperature. Sample 30% and 50% ZnO NPs was applied in lithium battery at voltage from 0.01 to 3. 1.2 mAhg-1 was recorded for sample 30% ZnO NPs while 100 mAhg-1.

Biosynthesis, Characterization, and Antibacterial Activity of Silver Nanoparticles Derived from Aloe barbadensis Miller Leaf Extract.

Background: There is a growing commercial demand for nano-formulations due to their prevalence applicability in various areas of bio-nanotechnology. Numerous chemical and physical methods have traditionally been used to synthesize silver nanoparticles, but they are limited due to use of toxic and harmful chemicals, thus drew researchers’ attention towards the biosynthesis of the silver nanoparticles by using medicinal plant.Objective: The present study enlightens the synthesis of silver nanoparticles in an echo-accommodating way by using aqueous Aloe vera leaf extract (AVLE) and evaluate its antimicrobial potential. Materials and Methods: The synthesis of silver nanoparticles using AVLE was determined by UV–vis spectrum and SEM. The optimization of different reaction conditions was measured, and antibacterial activity was evaluated by the disc diffusion method. Results: The optimum synthesis of AV-AgNPs showed at a 1mM concentration of silver nitrate, 595 ratio of AVLE to silver nitrate solution, pH 8 at ambient temperature for 24 hours. The synthesis was confirmed by UV–Vis spectroscopy maximum absorbance at 400 nm while SEM showed spherical morphology with an average particle size 20-24 nm. The antibacterial activity of AV-AgNPs was measured by disc diffusion method and exhibits significant antibacterial activity against both gram-positive and gram-negative bacteria. Conclusion: This method appears promising for the biosynthesis of silver nanoparticles by using Aloe vera with potent bactericidal activity, thus suggesting its role in clinical therapeutics and other fields.

A Comprehensive Study of the Neuropharmacological Profile of Methanol Leaf Extract of Aloe vera and Identification of Associated Neuroprotective Compounds through Gas chromatography-mass spectrometry Analysis

The objective of this study is to determine the neuropharmacological activities of the methanol extract of Aloe vera leaf and identify the potential bioactive compounds present in the same responsible for the activities through Gas chromatography–mass spectrometry analysis. The study is done on Wistar albino rats and composed of 5 groups, methanol extract of Aloe vera leaf at 200, 400, 800 mg/kg, normal saline (control) and standard drugs Phenytoin and Diazepam. Antiepileptic activities are done using maximal electroshock and pentylenetetrazole induced convulsion models and anxiolytic sedative activities using the staircase test, actophotometer test and sleep prolongation test. The chemometric analysis is performed using Clarus 500 Perkin Gas Chromatograph coupled with a mass detector, Turbo mass gold–Perkin Elmer Turbomass 5.1 spectrometer with an Elite-1 (100 % dimethyl polysiloxane), 30 m×0.25 mm ID×0.25 μm of the capillary column. The results demonstrated anxiolytic potential at 200 and 400 mg/kg and sedative activity at 800 mg/kg comparable to Diazepam. The methanolic extract of Aloe vera leaf at 800 mg/kg displayed antiepileptic activity compared to Phenytoin. The Gas chromatography–mass spectrometry analysis confirmed the presence of phytol (23.76), alpha-tocopherol (4.67), maltol (3.69), humulene (6.58), caryophyllene (2.73), n-hexadecanoic acid (2.16), hexadecanoic acid methyl ester (3.70), squalene (3.51), 9,12,15-octadecatrienoic acid methyl ester (2.12), which are antioxidant and anti-inflammatory compounds. These plant derived bioactive compounds are demonstrated to have antianxiety, antiepileptic, sedative hypnotic and neuroprotective potential in neurodegenerative models and might serve as future prospective drug candidates in neurodegenerative disorders).

Green synthesized CeO2 quantum dots: a study of its antimicrobial potential

Cerium oxide (CeO2) nanoparticles (NPs) of size ∼4 nm were successfully synthesized by green synthesis method using cerium nitrate as precursor and aloe-vera leaf extract as reducing and stabilizing agent. The switching between Ce3+ and Ce4+ state by reaction with H2O2 inferred the biomedical applications of CeO2 NPs. This switching enhances due to the presence oxygen vacancies and that was confirmed by photoluminescence spectrum. The SEM images showed spherical morphology of cubic fluorite structured CeO2 NPs which avoids the harming of cell. In the present study, CeO2 NPs show significant inhibition effect on gram negative bacteria, E. coli and P. aeruginosa which are responsible for food poisoning. Thus study analyze the synthesized CeO2 NPs as promising candidates for removal of biological pollutants and thus have great potential in the environmental remediation.

Catalytic Reduction of Organic Dyes with Green Synthesized Silver Nanoparticles using Aloe vera Leaf Extract

In this present study, we report a facile, cost-effective and non-toxic method of synthesis of silver nanoparticles using 4mM silver nitrate and aqueous leaf extract of Aloe vera as reducing and capping agent in presence of 25% ammonia solution. Then these green synthesized nanoparticles are used as catalysts for the degradation of organic dyes. The synthesized silver nanoparticles (AVSNPs) were characterized using different techniques like UV-Visible spectroscopy, Fourier infrared spectroscopy (FTIR), Dynamic light scattering (DLS), X-ray diffraction (XRD), Field emission-scanning electron microscopy (FESEM) and Energy Dispersive X-Ray Spectroscopy (EDAX). Further, the nanoparticles were seen to degrade organic dyes which were visibly seen and also recorded in UV-Visible Spectrometer. The initial synthesis of silver nanoparticles is confirmed by a peak at 417nm. The nanoparticles were found to be spherical in shape with an average size ranging between 11-23 nm. XRD analysis confirmed the formation of silver nanoparticles of crystalline nature and a face-centered cubic (fcc) lattice and FTIR analysis showed the involvement of the biomolecules in the reduction of silver to form silver nanoparticles and the EDAX confirmed the presence of silver nanoparticles. The green synthesized nanoparticles catalyze the degradation of different organic dyes in a very short period of time. The green synthesized silver nanoparticles were seen to show excellent activity in catalyzing the degradation of organic dyes in an aqueous medium in the presence of the electron donor sodium borohydride (NaBH 4 ). Keywords: Aloe vera, catalysis, dye reduction, green synthesis, NaBH4, organic dyes, silver nanoparticles

RADIOPROTECTIVE EFFECTS OF ALOE VERA ON HEPATOSOMATIC INDEX OF SWISS ALBINO MICE

Objective: In the living organisms, deleterious effects produced by ionizing radiations. Human exposure to ionizing radiations increased enormously because of rapid technological advancements. There is a need to protect humans against such effects of ionizing radiation. Protection against the deleterious effects of ionizing radiations by radioprotectors was studied, which may be of great help for human application. Present study was conducted to evaluate the modulating efficacy of prolonged administration of Aloe vera extract against gamma irradiation-induced toxicity in mice.&#x0D; Methods: Animals were given Aloe vera leaf extract orally 1000 mg/kg body weight/d for 15 consecutive days before radiation exposure (0.5, 3 and 5.5 Gy gamma radiation).&#x0D; Results: Mice were autopsied at day ¼, 1, 3, 5, 10 and 20 after irradiation to evaluate the radio modulator effect in terms of the hepatosomatic index.&#x0D; Conclusion: Aloe vera extract has a beneficial protective effect against radiation-induced oxidative stress.

Rare Earth Element (REE) Nd3+ Doped CeO₂ Nanoparticles Using Aloe vera Leaf Extract: Structural, Optical and Antimicrobial Activity.

Pure and Nd-doped cerium oxides (CeO₂, Ce0.075Nd0.025O and Ce0.050Nd0.050O) nanoparticles (NPs) were synthesized using Aloe vera leaf extract. X-ray powder diffraction studies were confirmed that synthesized pure and Nd3+ doped CeO₂ NPs and were exhibited cubic structure. FE-SEM image exhibited pure and Nd3+ doped CeO₂ NPs have spherical structure and Nd doped CeO2 NPs small pores were formed in the surface. EDAX spectral analysis used to identify the elemental compositions. FT-IR spectra shows various functional groups and were identified for pure and Nd3+ doped CeO₂ NPs. UV-Vis spectra, absorption edges were observed at 314 nm, 324 nm and 357 nm for pure and Nd doped CeO2 NPs respectively. PL spectral analysis, small shift observed for emission of the CeO2 NPs values as compared to that of the Nd doped CeO2 NPs emission values. The antimicrobial studies were performed against a set of Gram positive (Staphylococcus aureus and Streptococcus pneumoniae) and Gram negative (Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, Klebsiella pneumonia and Shigella dysenteriae) bacterial and fungal candida albicans strain.

Comparing the Effects of Aloe Vera Leaf Extract On K562 Tumor Cell and Peripheral Blood Mononuclear Cells

Aims Aloe Vera is among the crucial medicinal plants, with proven anti-cancer effects. This study aimed to investigate the effect of plant extracts of Aloe Vera Leaf (AVL) on the proliferation of cell lines K562 (erythroleukemia) and Peripheral Blood Mononuclear Cells (PBMCs). Methods &amp; Materials The inner parts of the plant, including the gelatinous substance, were emptied, and the outer layer was used to prepare the lyophilized extract. The extract was dissolved in DMSO. K562 cells and PBMCs were cultured with 9.375, 18.75, 37.5, 75, 150, and 300 μg/mL concentrations of AVL for 24, 48, or 72 hours. After cultivation, the IC50 was determined for the K562 and normal PBMCs, using the MTT assay (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide). Doxorubicin was used as a positive control. Findings The obtained data suggested that the viability of cells in both the leukemic cell line and normal PBMCs significantly decreased by the treatment with the extract in a dose-dependent manner; however, the AVL toxicity for PBMC was significantly more than K562. IC50 for the standard drug was also significantly less than AVL extract. Conclusion AVL possesses cytotoxic effects for K562 and PBMCs. Nevertheless, it has no selective benefits and has more cytotoxic effects on PBMCs.

Aloe vera leaf extract-assisted facile green synthesis of amorphous Fe2O3 for catalytic thermal decomposition of ammonium perchlorate

Fe2O3 is a well-known catalyst for thermal decomposition of ammonium perchlorate (AP). Amorphous Fe2O3 nanoflakes were biosynthesised by a novel sol–gel method using Aloe vera leaf extract, and its catalytic effect on thermal decomposition of AP was investigated. The precursor ferric nitrate–Aloe vera gel was calcined at three different temperatures. The oxides were characterised by Fourier-transform infrared spectroscopy, X-ray powder diffraction, atomic force microscopy, field emission scanning electron microscopy and transmission electron microscopy. Microscopy analyses revealed that Fe2O3-250 consists of a porous structure as aggregates of many nanoflakes of ~ 2 nm diameter. The catalytic activity of the synthesised products on thermal decomposition of AP was evaluated using thermogravimetry–differential scanning calorimetry. The presence of 2.0% of mesoporous Fe2O3-250 catalyst (surface area of 208 m2 g−1) enhanced the rate of high-temperature decomposition of AP by decreasing the peak decomposition temperature from 367 to 337 °C. Average activation energy of thermal decomposition of AP was calculated using Flynn–Wall–Ozawa method reduced from 145 to 128 kJ mol−1 in the presence of the catalyst. The high surface area of mesoporous Fe2O3-250 originated from its amorphous nature makes it a potential catalyst for thermal decomposition of AP.

An experimental study to test the haemostatic effect of Aloe vera

Background: Blood clotting is a process which prevents blood loss during injuries. Blood clots even when it is coming out from the blood vessels. Aloe vera is a perennial plant found all over India. It is commonly used in traditional system of medicine for treatment of wound healing, mouth ulcers, constipation, skin wrinkles and diabetes mellitus It is also used as antioxidant and antimicrobial agent. As it is used in traditional system of medicine for wound healing, this study was under taken to test the possible haemostatic effect of aloe vera.Methods: 12 rats were divided into two groups (control and test) with 6 rats in each group. Determination of Bleeding Time (BT), rat tail was warmed for one minute in water at 40˚C and then dried. A small cut was made in the middle of the tail with a scalpel. In test group, a drop of aloe vera leaf extract was applied on the injured area immediately after making the cut in the middle of the tail, where as in the control group nothing was applied, and BT estimated. Determination of Clotting Time (CT), 12 test tubes were arranged in water bath at 37˚C. Control Group: 0.4 ml of blood was collected from each rat in the control group and added to 6 test tubes kept in the water bath. Test group: For the remaining 6 test tubes 0.1 ml of aloe vera leaf extract was added. 0.4 ml of blood collected from the test group was added to these test tubes. The CT was estimated for both control group and test group.Results: The results were statistically analyzed by using unpaired t-test. The reduction in BT and CT for test group was statistically highly significant (p&lt;0.001) compared to control group.Conclusions: In this study aloe vera leaf extract significantly reduced both BT and CT in the test group.