In order to improve the efficiency of online consumption and supply chain efficiency of fresh agricultural products in the e-commerce environment, a network node layout optimization algorithm was designed. In the e-commerce environment, a network node deployment model has been constructed for the online consumption behavior of fresh agricultural products. A network transmission supply chain detection model was established through the rotation scheduling method. By combining distributed transformation protocols, the spatial layout characteristics of network nodes have been reorganized. Further introduction of a multi-coverage scheduling model, combined with sensor information fusion tracking technology, accurately detects the layout characteristics of network nodes. Based on these detection data, forwarding and adaptive control protocols were developed. Real-time control using the node traversal method was developed. Under the optimal node supply chain transmission mechanism, the scheduling of the network node layout has been achieved. Meanwhile, convolutional neural networks were utilized to dynamically adjust layout control to ensure convergence and stability. The simulation results show that the node forwarding ability of the network node layout design for online consumption behavior of fresh agricultural products in the e-commerce environment is good, which improves the output balance of the network supply chain transmission of online consumption behavior of fresh agricultural products in the e-commerce environment. In addition, the optimized delivery cost decreased by 20% compared to before optimization, delivery efficiency increased by 30%, and inventory turnover rate increased by 25%, confirming that the proposed method has good optimization performance.