Lactobacillus Plantarum IS-10506 Research Articles

Effect of Lactobacillus Plantarum IS-10506 on Paneth cell regeneration in the ileum of Sprague dawley rats

Background: Escherichia coli (E. coli) is the most common infectious agent among children in developing countries. Local probiotics are not widely used to treat diarrhea and intestinal infections. This study aims to investigate the cell regeneration process of Paneth cells after administration of Lactobacillus Plantarum IS-10506 due to damage caused by Lipopolysaccharide (LPS) E. coli O55:B5, through the expression of MATH-1 and DEFA6. Methods: This study used 64 paraffin blocks from Rattus norvegicus strain Spraque- Dawley divided into four groups. The KN group were administered a placebo. The KL group received LPS E. coli O55:B5 on day one. The KP group received LPS E. coli O55:B5 on the first day and Lactobacillus plantarum IS-10506 on the second day until six day. The KPR group were given Lactobacillus plantarum IS- 10506 six days prior to receiving LPS E. coli O55:B5. All groups, except KN, received LPS at a dose of 250 μg/kg body weight once, and Lactobacillus plantarum IS-10506 at a dose of 2.86x106 CFU/day. Evaluating Paneth cell regeneration, DEFA6, and MATH-1 expression immunohistochemistry was conducted on all tissues. Results: The expression of DEFA6 and MATH-1 in the KP and KPR groups on day three of observation was significantly different from the KL group. Even though the KL group achieved significant growth, the results of this expansion were significantly smaller than KP and KPR groups. Conclusion: After mucosal injury caused by LPS E. coli 055:B5, administration of probiotic Lactobacillus plantarum IS- 10506 may increase Paneth cell regeneration through differentiation and cell number.

Effects of Lactobacillus plantarum IS-10506 on gastric protective mucosal factors: an ibuprofen-induced gastric mucosal injury

Background: Gastric mucosal protective factor plays an important roles in gastric homeostasis between mucosal injury and mucosal repair. Previous study implicated probiotics in the repair of the intestinal mucosa, while probiotic role in gastric mucosa remains poorly understood. This study aims to determine the role of probiotic L. plantarum IS-10506 in gastric protective mucosal factors by examining the expression of cyclooxygenase-1 (COX-1), COX-2, prostaglandin E2 (PGE2), and gastric mucus production (Mucin 5AC/ MUC5AC) by surface mucous cells as gastric mucosal protective factors. Methods: Twenty-seven male Wistar rats (Rattus norwegicus) were divided into 3 groups, ie. control, curative and preventive group. Ibuprofen 300mg/kgBW were given to all groups to induce gastric injury, while Lactobacillus plantarum IS-10506 1x109 CFU/day were given into the curative (5 days) and preventive (12 days) groups. Antrum tissue with primary monoclonal antibodies againts COX-1, COX-2, PGE2, and MUC5AC were examined in the day -1, -3, and -5 after necropsy. Results: Number of cells expressed COX-1, COX-2, PGE2 and MUC5AC in curative and preventive groups were significantly higher than control group. L. plantarum IS-10506 showed its efficacy in gastric mucosal repair on third day in the curative group and on first day in the preventive group. L. plantarum IS-10506 probiotic plays a role in gastric protective mucosal factors by promoting cell expression of COX-1, PGE2, and MUC5AC. In addition, it has been determined that L. plantarum IS-10506 inhibits COX-2 expression, that may lead to minimalize the inflammatory conditions and a deterioration of the gastric mucosal environment. Conclusion: Lactobacillus plantarum IS-10506 can prevent ibuprofen-induced antral mucosal damage through gastric protective mucosal factors.

Lactobacillus plantarum IS-10506 Accelerates Healing of Gastric Injury Induced by Ketorolac in Wistar Rats

Gastric injury is an event that often occurs due to many factors, such as the use of drugs, stress factors, infections, chemicals, etc. The use of histamin 2(H2) receptor antagonist drugs and pump inhibitors have become the choice for gastric injury treatment so far and requires a relatively long time. The widespread use of probiotics has been shown to affect the healing process of digestive tract disorders, for example in the small intestine. This study aimed to investigate the effect of Lactobacillus plantarumIS-10506 in acceleratingthe healing of gastric injury induced by ketorolac in the rat. The experimental study used 64 Wistar rats divided into 4 groups, group 1 (control), group 2(ketorolac administration), group 3 (ketorolac and probiotic administration), and group 4(preventive treatment with probiotic before, ketorolac administration, and treatment with probiotic). Each group was divided into 4 subgroups based on the day of sacrifice, days 1, 5, 7, 10. The healing of gastric injury evaluating by epithelial defects improvement and fibroblast cells by hematoxylin and eosin (HE) staining.The group induced by ketorolac (group 2) showed the highest epithelial defect score (p=0.048) on day 1. The repair of the epithelial defect in group 3 and group 4 were significantly increased on day 5, while group 2remains defectiveon day 5(p=0.019). Fibroblast cells of groups 3 and 4 decreased significantly more than others on day 10(p=0.024). Lactobacillus plantarum IS-10506 influences the healing acceleration of gastric injury by ketorolac by enhancing epithelial regenerationand fibroblast cells.

Correlation between disease severity and quality of life index in psoriasis patients treated with Lactobacillus plantarum IS-10506

Psoriasis is a chronic and relapsing inflammatory skin disease that affects both genders at all ages. The purpose of this study is to identify the correlation between psoriasis area severity index (PASI) and Dermatology Life Quality Index (DLQI) for patients with plaque psoriasis before and after treatment with Lactobacillus plantarum IS-10506. This was a before after cohort study. Adults with mild to moderate psoriasis were recruited into the study. All participants received the original Indonesian probiotic strain Lactobacillus plantarum IS-10506 at a dose of 2x1010 colony forming units (CFU) per day for six weeks. A total of 22 patients, with a mean age of 43 years, participated in the study. Participants had psoriasis for an average of 14 years. There was a correlation between PASI score and several quality-of-life questionnaire elements in certain condition, such as professional jobs, unemployment, obesity, and joint symptoms. The correlation between particular conditions reveals that occupations, obesity, and joint symptoms have a greater impact on psoriasis patients.

​The Downregulation of Pro-inflammatory Cytokine Interleukin-6 after Oral Administration of Lactobacillus plantarum IS-10506 in Mice Model for Endometriosis

Background: As a multi-functional cytokine, interleukin-6 (IL-6) is advanced in endometriosis. Probiotics are known to be able to modulate/regulate immune responses through downregulation of the pro-inflammatory cytokine IL-6. Lactobacillus plantarum IS-10506 (LIS-10506) is a novel probiotics strain derived from curd (a milk product which look like a yogurt) from traditionally made from buffalo milk or usually called by dadih from West Sumatra, Indonesia. This investigation intended to comprehend the impact of LIS-10506 administration on IL-6 expression in the endometrial tissue, serum IL-6 levels and endometriosis implant areas in mice model for endometriosis. Methods: This study conducted at the Animal Laboratory, Professor Nidom Foundation, Surabaya, Indonesia from January to June 2021. This experimental study was conducted on 32 adult female mice 12 weeks old divided into 4 groups in a completely randomized design with a posttest only control group design. LIS-10506 microencapsulation of curd was administered orally at a dose of 109 CFU/mice/day for 7 and 21 days. Serum IL-6 levels were assessed using IL-6 ELISA Kit for mice, IL-6 expression was assessed semi quantitatively according to the Remmele method and implant areas were measured by the area of hyperemia in mice’s peritoneal wall. Result: This investigation revealed that the peritoneal IL-6 expression and implant area experienced a decrease. In summary, the administration of LIS-10506 could downregulate pro-inflammatory cytokine IL-6. This present study has demonstrated the effect of LIS-10506 on one of the pathophysiological pathways in endometriosis.

Regulation of T Helper and Regulatory T Cells by Lactobacillus Plantarum IS-10506 Supplementation to Human Immunodeficiency Virus-Infected Children Under Antiretroviral Therapy

Human immunodeficiency virus infection is associated with impairment of T helper 1, T helper 2, regulatory T, and T helper Type 17 homeostasis. While probiotics are widely known to improve these changes, understanding of their role on children’s immune system remains limited. In a randomized, double-blind, and placebo-controlled study conducted in Dr. Soetomo General Hospital, Surabaya from December 2012 to March 2013, we have investigated the effect of Lactobacillus plantarum IS-10506 on the regulation of T helper and regulatory T cell in children infected with human immunodeficiency virus receiving antiretroviral therapy for at least 6 months. Twenty-one human immunodeficiency virus infected children were divided into placebo and probiotic groups and L. plantarum IS-10506 was administered at a dose of 2.86 × 1010 colony forming units/day for 6 weeks. Levels of interferon-γ, interleukin-4, transforming growth factor-β, and interleukin-17 were analyzed before and after treatment as an indicator of T-cell regulation of T helper and regulatory T cells. The results show a significant increase in transforming growth factor-β level ( p = 0.003) after administration of L. plantarum IS-10506 compared to placebo with the first-line antiretroviral therapy group. There was no reported adverse effect in this study. In conclusion, L. plantarum IS-10506 increases the regulation of regulatory T cell in human immunodeficiency virus infection in children receiving first-line antiretroviral therapy treatment.

Probiotic Lactobacillus plantarum IS-10506, Expression of Glial Fibrillary Acidic Protein and Platelet Endothelial Cell Adhesion Molecule-1 by Astrocytes and Endothelial Integrity: The Importance of Intestinal Microbiota as Blood Brain–Barrier Stabilizer

Gut microbiota is a complex community that helps maintain the dynamic metabolic ecological balance of the brain through the gut–brain axis and keeps the blood–brain barrier structure intact. However, the knowledge of how the gut microbiota responds to exogenous influences on the blood–brain barrier structure remains limited. This study hypothesizes that probiotic Lactobacillus plantarum IS 10506 supplementations could ameliorate the disruption of the blood–brain barrier structure. To this end, we examined effect of the probiotic L. plantarum IS 10506 on the expression of glial fibrillary acidic protein and platelet endothelial cell adhesion molecule-1 in the control and E. coli serotype O55:B5 lipopolysaccharide treated blood–brain barrier disruption model of Wistar rats. The rats receiving L. plantarum IS 10506 alone or along with E. coli serotype O55:B5 lipopolysaccharide exhibited upregulation of the expression of glial fibrillary acidic protein and platelet endothelial cell adhesion molecule-1. In conclusion, the probiotic L. plantarum IS-10506 stimulates the restoration of blood–brain barrier disruption.

Effect of Probiotic on Repair of Ethanol-induced Gastric Mucosal Erosion in Wistar Rats

Background: Probiotics can increase mucus secretion as a mechanism to improve barrier function and pathogen exclusion. Several Lactobacillus species can increase mucin expression in human intestinal cells. Lactobacillus Plantarum IS-10506 is a probiotic native to Indonesia derived from curd, fermented milk from Sumatra. Research with Lactobacillus Plantarum IS-10506 showed a protective effect as well as a therapeutic effect on intestinal mucosal damage to the ileum. Objective: Analyzing the effect of probiotic Lactobacillus Plantarum IS-10506 on the acceleration of ethanol-induced gastric mucosal erosion regeneration. Method: Experimental research used 48 rats that were fasted and divided into 3 groups those are K1 group control group, group K2 which was treated with ethanol 43%, and group K3 which was treated with ethanol and probiotic Lactobacillus Plantarum IS-10506. Each group was divided into 4 subgroups based on the day of the sacrifice those are days 1, 3, 5, and 7. The treatment of the test animals is done in 7 days. Epithelial defect was measured by using histopathologic scoring by Roger’s Results: The score of Epithelial defect Group K2 was significantly different compared to group K1 (p=0.036), and K3 (p=0.007). The gastric mucosal repair was completed in day 7 in the probiotic group. Conclusion: Probiotic Lactobacillus Plantarum IS-10506 accelerate mucosal repair in etanol induced gastric mucosal injury

Effect of Probiotic on the improvement of Ibuprofen-induced Gastric Mucosal Injury Model in Wistar Rats

Introduction: Gastric mucosal injury is a situation that is often obtained as a result of various conditions in humans, both adults and children. Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to cause gastric mucosal injury as a side effect. Ibuprofen is the most commonly used and most frequently prescribed NSAID. In this study, the probiotic used is from the lactobacillus strain called Lactobacillus Plantarum IS-10506. The aim of this study is to determine the effect of probiotic on repairing the ibuprofen-induced gastric mucosal injury. Methods: Thirty-two Wistar rats were divided into 2 groups: Ibuprofen group (K1), which received Ibuprofen then the second group (K2) which received probiotics LIS-10506 for 7 days after being given Ibuprofen. Necropsy was done on days 1, 3, 5 and 7 with gastric as unit analysis. The microscopic examination was conducted to evaluate the structure of gastric mucosal measured based on the Histology Activity Index (HAI) by Rogers (2012). The statistical analysis used Kruskall Wallis, Mann-Whitney test, p< 0.05 was considered statistically significant. Results: None of the research subjects had dropped out of the test during the observation period. The epithelial damage in between groups K1 and K2 there was no significant difference on the first day (p=0.456), third (p=0.456), fifth (p=0.099), but on the seventh day there was a significant difference (p=0.034). Conclusion: Probiotic LIS-10506 has an effect on the repair of gastric mucosal damage induced by ibuprofen.

Shelf life of Microencapsulated and Free Cells of Lactobacillus plantarum IS-10506 at Different Temperatures

Survival of microencapsulated probiotic Lactobacillus plantarum IS-10506 ofdadih origin at different storage conditions has been investigated. L. plantarum cells suspension was dispersed in Flocel pH 101 mixed with skim milk powder and dried by Fluid Bed Dryer (FBD) at 37°C for 2 hours, then microencapsulated by 4.75% (w/v) sodium alginate and 5.5% (w/v) calcium chloride. The microencapsulated and free cells probiotics were packed in standing aluminium pouch and stored at three different temperatures, 24°C (room temperature), 4°C (chiller), and-20°C (freezer) for 24 weeks, and the viable counts of probiotic were enumerated using MRS Agar medium every two weeks. Microencapsulated and free cells of probiotic Lactobacillus plantarum IS-10506 showed stable viability at -20°C for 24 weeks. At 4°C free cells showed significant decreased of viability while microencapsulated cells showed stable viability at week 24. Storage at 24°C led to significant loss of viability (p< 0.05) of both free cells and microencapsulated cells after 8 and 10 weeks, respectively, to undetectable level. Moisture content in both of free cells and microencapsulated cells at 24° C significantly increased at week 2. While at 4°C, there was significant increase of moisture content observed in free cells at week 2, and microencapsulated cells showed significant increase of moisture content at week 10. Likewise, at -20°C, moisture content of both free and microencapsulated cells showed significant increase at week 2 and 10, respectively.

Beneficial effect of Lactobacillus plantarum IS-10506 supplementation in adults with atopic dermatitis: a randomized controlled trial

Introduction Although the therapeutic effects of probiotics in atopic dermatitis (AD) are known, the limited findings in adults are inconsistent. Lactobacillus plantarum (LP) IS-10506 was found to improve AD symptoms due to its immunomodulatory effects. Objective To assess the Scoring Atopic Dermatitis Index (SCORAD), the serum immunoglobulin E (IgE), interleukin (IL)-4, interferon-gamma (IFN-γ), forkhead box P3 (Foxp3+), and IL-17 levels in adults with mild and moderate AD after LP IS-10506 supplementation. Methods A randomized double-blind placebo-controlled trial comparing the microencapsulated probiotic (2 × 1010 CFU/day) and placebo (skim milk-Avicel) was conducted at an outpatient clinic on 30 adults with mild and moderate AD. The patients were divided into 2 groups with 15 patients each: intervention and control. Result The SCORAD score was significantly lower in the probiotic than the placebo group on the 8th week. The IL-4 and IL-17 levels were significantly lower in the probiotic than the placebo group. The IFN-γ and Foxp3+ levels were significantly higher in the probiotic than the placebo group. However, the IgE levels remained significantly unchanged. Conclusion The administration of LP IS-10506 is effective for alleviating AD symptoms in adults owing to its immunomodulatory effects.

Physio-chemical, Microbiology, and Preference of Probiotic Fresh Soft Cheese Using Lactobacillus plantarum IS-10506 and Streptococcus thermophilus as Mixed Starter Culture

Probiotics are live microorganisms with various benefficial effect on human health when consumed in adequate amount. Soft cheese is one of fermented milk products involving lactic acid bacteria (LAB) which may be potential as functional food. Concentration ratios of starter culture Lactobacillus plantarum IS-10506 and Streptococcus thermophilus were 1:1, 2:1, and 1:2 (v/v) at 5%, 10%, and 15% (v/v) at 106 CFU mL−1 cultures and incubated at 37 °C for 90 minutes. Chemical (pH and moisture content), microbiology (LAB total count), and sensory (preference test) were analyzed. The results showed that there was no significant difference (p-value < 0.05) on the viability of LAB (in a range of 6×107 to 1.71×108 CFU mL−1) and on cheese yield (224-268.84 g L−1). Cheese fermented with 1:1 (v/v) Lactobacillus plantarum IS-10506 and Streptococcus thermophilus of 10% cultures (v/v) was the most preferred, scored 3.75 out of 5 in hedonic test for overall acceptability and the viable LAB was 1.71x108 CFU mL−1 with pH 4.95. The total yield was 257.54 g L−1 and its moisture content was 66.45%.

The Effect of Different Starter Cultures and Dextrose on Viability of Lactic Acid Bacteria and pH of Fermented Milk At 43 °C

The purpose of this research was to study the effect of different starter cultures and the addition of dextrose on viability of LAB and pH value of fermented milk. Streptococcus thermophilus and Lactobacillus bulgaricus, Streptococcus thermophilus and Lactobacillus plantarum IS-10506 were used as mixed starter cultures, incubated at 43°C for 8 hours, with or without the addition of dextrose. The total viable counts of LAB and pH value were assessed. Each combination of starter cultures showed significant increased on the viable counts of LAB, and decreased the pH value of fermented milk during 8 hours incubation. Addition of 2% dextrose tended to increase viable counts of LAB by 165.8 % of milk fermented with mixed starter cultures of S. thermophilus and L. plantarum IS-10506, extending log phase from 6 to 8 hours, while without 2 % dextrose tended to decreased by 40 %. No significant effect of addition of 2% dextrose on viable counts of yogurt starters in yogurt. Mixed starter cultures of S. thermophilus and L. plantarum IS-10506 is potential starter cultures of fermented milk with addition of 2 % dextrose at 43°C for 8 hours incubation time to produce probiotic based functional drink.

THE EFFECTS OF LACTOBACILLUS PLANTARUM IS-10506 ON ACCELERATING THE REGENERATION OF THE INTESTINAL MUCOSA STRUCTURE EXAMINED BY THE SCANNING ELECTRON MICROSCOPE

Lactobacillus plantarum IS-10506 (LIS-10506) is a native Indonesian probiotic derived from fermented milk originating from Sumatera. Intestinal mucosa damage results from gastrointestinal tract infections. Using Scanning Electron Microscopy (SEM), the intestinal mucosa structure, following mucosal damage, was evaluated. The objective of this study to evaluate effect of Lactobacillus plantarum IS-10506 on accelerating the regeneration of the intestinal mucosa structure, using SEM. This study conducted on sixty-four Sprague-Dawley rats were divided into 4 groups: control group (K1); the group that received Lipopolysaccharide (LPS) Escherichia coli O55:B5 (K2); the curative group, which received LPS Escherichia coli O55:B5 then probiotics (K3); the preventive group, which received probiotics six days before LPS Escherichia coli O55:B5 and continued probiotics (K4). The probiotics used were LIS-10506. Necroscopy was performed on days 3, 4, 6 and 7, and the ileum was analysed. SEM was conducted to evaluate the structure of the intestinal mucosa. SEM showed that the K4 group experienced the fastest mucosal regeneration on the fourth day. On the seventh day, examination of the ileum using SEM showed improvement in the intestinal villi of all groups. The conclusion, SEM showed that all groups underwent regeneration following mucosal damage. Probiotics accelerated the regeneration of the intestinal mucosa, and the best results were observed in the preventive group.

Lactobacillus plantarum IS-10506 promotes renal tubular regeneration in pyelonephritic rats.

Lactobacillus plantarum IS-10506 is a novel probiotic isolated from Indonesian traditional fermented buffalo milk dadih. Probiotics are clinically proven to be effective in accelerating recovery after urinary tract infection (UTI) and in decreasing recurrent UTI in children with or without structural disorders of the urinary tract. This study aimed to investigate the role of the probiotic L. plantarum IS-10506 in activating and regenerating renal epithelial stem cells in pyelonephritic rats. Fifty-five 20-weeks-old male Sprague-Dawley rats were randomised into control, lipopolysaccharide (LPS) and treatment (LPS and probiotic) groups. Pyelonephritis was induced with Escherichia coli ATCC 25922 LPS administered via a urethral catheter on the first experimental day to the LPS and treatment groups. Microencapsulated L. plantarum IS-10506 was orally administered once daily for up to 14 days to the treatment group. On days 3, 5, 7, 10 and 14, five rats per group were sacrificed and their kidneys were immunohistochemically analysed for production of interleukin (IL)-10, lipocalin-2 and Ki-67 in the renal tubular cells. IL-10 production was upregulated starting from day 3 through day 14 in the treatment group (P<0.05) compared with that in the control and LPS groups. Moreover, treatment with the probiotic led to increased activation of renal tubular stem cell, as indicated by a higher lipocalin-2 production, and further proliferation of renal tubular stem cells was documented by a higher Ki-67 production. Taken together, these results indicate that the anti-inflammatory probiotic L. plantarum IS-10506 improves renal injury in pyelonephritic rats by activating endogenous renal tubular stem cells to proliferate into mature renal tubular epithelial cells.

Effect of Lactobacillus plantarum IS-10506 on blood lipopolysaccharide level and immune response in HIV-infected children

HIV enteropathy may cause disruption of the intestinal barrier, leading to a loss of CD4+ T cells, increased intestinal permeability, and microbial translocation. Lactobacillus plantarum IS-10506 has the ability to improve gut barrier function. This study investigated the effect of L. plantarum IS-10506 on a number of biomarkers of enteropathy-related damage in HIV-infected paediatric patients undergoing antiretroviral therapy (ARV). A randomized, double-blind, placebo-controlled study was conducted on 2-18 year-old children, diagnosed as HIV infected according to the WHO 2007 criteria who had received ARV for ≥ 6 months. Subjects were excluded if ARV therapy was discontinued or the patients took probiotics ≥ 2 weeks prior to the study or during the study period. Subjects were randomized into a probiotic group and placebo group. The probiotic group received L. plantarum IS-10506 2.86 × 1010 cfu/day for 6 days. Blood lipopolysaccharide (LPS) level, serum CD4+ T cell count, serum CD8+ T cell count, CD4+/CD8+ T cell ratio, and faecal sIgA level were assessed as biomarkers. Twenty-one subjects completed this study. The blood LPS level decreased significantly in the probiotic group (p = 0.001). There was no significant difference in absolute CD4+ T cell count, percent CD4+ cells, absolute CD8+ T cell count, CD4+/CD8+ T cell ratio, or faecal sIgA. No serious adverse events were reported. The probiotic L. plantarum IS-10506 reduced the blood LPS level but showed no effect on the humoral mucosa and systemic immune response in HIV-infected children undergoing ARV therapy.

Effect of the probiotic Lactobacillus plantarum IS-10506 on BDNF and 5HT stimulation: role of intestinal microbiota on the gut-brain axis

Microbial communities residing in the gut play a major role in the communication between the gut and the brain through neural, immune, and hormonal routes. Changes in abundance of beneficial intestinal bacteria can affect health of individuals. Conversely, drugs, disease, diet and other factors can alter the gut microbiome. However, there is limited information on the effect of exogenous factors on gut microbiota. In this study, we investigated whether a beneficial bacterium, the probiotic Lactobacillus plantarum IS-10506, can stimulate the gut-brain axis using Wistar rats. The animals were divided into two groups: one received L. plantarum IS strain 10506 supplementation, while the control group received no treatment. Activation of the gut-brain axis was evaluated by immunohistochemical analysis of intestinal and brain serotonin (5-HT) and brain neurotrophin (NT), serotonin transporter (5-HTT), and brain-derived neurotrophic factor (BDNF) levels. The results showed that BDNF (p< 0.000), NT (p< 0.000007), and 5-HTT (p< 0.000007) expression was upregulated in the brain along with intestinal 5-HT (p< 0.000) level in rats treated with L. plantarum strain IS-10506 relative to the control group. The probiotic L. plantarum IS-10506 stimulates the gut-brain axis and can potentially promote brain development and function.

Effect of Paraprobiotic Lactobacillus Plantarum IS-10506 on Humoral Immune Response of Elementary School Children

Effect of Paraprobiotic Lactobacillus Plantarum IS-10506 on Humoral Immune Response of Elementary School Children

Lactobacillus plantarum IS-10506 probiotic administration increases amlodipine absorption in a rabbit model.

ObjectiveProbiotics are beneficial in human health. In this study, we investigated the effect of probiotics on absorption of amlodipine, a dihydropyridine calcium antagonist used in the treatment of angina and hypertension, in a rabbit model.MethodsLactobacillus plantarum IS-10506 probiotic was administered for 14 days to male New Zealand rabbits. Blood samples were collected before and after probiotic supplementation. Amlodipine (10 mg) was then administered to all groups. Blood samples from a marginal vein were withdrawn at 5, 15, 30, 60, and 120 minutes to determine amlodipine concentrations in rabbit plasma.ResultsAmlodipine concentrations in the L. plantarum IS-10506 group were 4.95 ± 1.22, 8.71 ± 0.69, and 12.48 ± 2.53 ng/ml, and those in the control group were 1.69 ± 0.31, 3.89 ± 1.23, and 7.17 ± 1.85 ng/ml at 30, 60, and 120 minutes, respectively after administration of amlodipine. Amlodipine concentrations in the L. plantarum IS-10506 group were significantly higher than those in the control group at 30, 60, and 120 minutes after amlodipine administration.ConclusionOur results suggested that supplementation of L. plantarum IS-10506 significantly increases amlodipine plasma concentrations in rabbits.

Identification of Dysbiosis Related Bacteria from New Zealand’s White Rabbit Intestinal Treated With Lactobacillus plantarum IS-10506 as Probiotics Food Supplementation

The gastrointestinal microoganisms encompass thousands of bacterial species that constitute a relatively stable ecosystem inside human body. Dysbiosis is an imbalance condition of beneficiary microbacteria cause by newer microorganism. This research aimed to investigate the effect of L. plantarum IS-10506 as probiotics supplementation on total bacterial and Enterobacteriaceae count; and also to identify the dysbiosis causing bacteria. Total bacterial and Enterobacteriaceae number on both control and test group were determined by Total Plate Count method. Identification of dysbiosis related bacteria were determined by fermentation test, gram staining, and Microbact™ 12A kit. The TPC result of total bacterial population on control group was 222.67 x 106 cfu/ml, while the probiotics induced group was 210.33 x 106 cfu/ml. As for the TPC result for Enterobacteriaceae population from control group was 12.00 x 106 cfu/ml, Enterobacteriaceae population in the probiotics induced group was 11.66 x 106 cfu/ml. Four genera related to dysbiosis has been isolated from the intestinal sample and all are rod-shaped Gram negative bacteria. In conclusion, the use of L. plantarum IS-10506 as food supplementation has reduced the total bacterial count. Four genera of dysbiosis related bacteria has been identified from the intestinal sample which include Eschericia, Serratia, Enterobacter, and Citrobacter.