Effect of Probiotic on the improvement of Ibuprofen-induced Gastric Mucosal Injury Model in Wistar Rats

Abstract

Introduction: Gastric mucosal injury is a situation that is often obtained as a result of various conditions in humans, both adults and children. Nonsteroidal anti-inflammatory drugs (NSAIDs) are known to cause gastric mucosal injury as a side effect. Ibuprofen is the most commonly used and most frequently prescribed NSAID. In this study, the probiotic used is from the lactobacillus strain called Lactobacillus Plantarum IS-10506. The aim of this study is to determine the effect of probiotic on repairing the ibuprofen-induced gastric mucosal injury. Methods: Thirty-two Wistar rats were divided into 2 groups: Ibuprofen group (K1), which received Ibuprofen then the second group (K2) which received probiotics LIS-10506 for 7 days after being given Ibuprofen. Necropsy was done on days 1, 3, 5 and 7 with gastric as unit analysis. The microscopic examination was conducted to evaluate the structure of gastric mucosal measured based on the Histology Activity Index (HAI) by Rogers (2012). The statistical analysis used Kruskall Wallis, Mann-Whitney test, p< 0.05 was considered statistically significant. Results: None of the research subjects had dropped out of the test during the observation period. The epithelial damage in between groups K1 and K2 there was no significant difference on the first day (p=0.456), third (p=0.456), fifth (p=0.099), but on the seventh day there was a significant difference (p=0.034). Conclusion: Probiotic LIS-10506 has an effect on the repair of gastric mucosal damage induced by ibuprofen.