Shelf life of Microencapsulated and Free Cells of Lactobacillus plantarum IS-10506 at Different Temperatures

Abstract

Survival of microencapsulated probiotic Lactobacillus plantarum IS-10506 ofdadih origin at different storage conditions has been investigated. L. plantarum cells suspension was dispersed in Flocel pH 101 mixed with skim milk powder and dried by Fluid Bed Dryer (FBD) at 37°C for 2 hours, then microencapsulated by 4.75% (w/v) sodium alginate and 5.5% (w/v) calcium chloride. The microencapsulated and free cells probiotics were packed in standing aluminium pouch and stored at three different temperatures, 24°C (room temperature), 4°C (chiller), and-20°C (freezer) for 24 weeks, and the viable counts of probiotic were enumerated using MRS Agar medium every two weeks. Microencapsulated and free cells of probiotic Lactobacillus plantarum IS-10506 showed stable viability at -20°C for 24 weeks. At 4°C free cells showed significant decreased of viability while microencapsulated cells showed stable viability at week 24. Storage at 24°C led to significant loss of viability (p< 0.05) of both free cells and microencapsulated cells after 8 and 10 weeks, respectively, to undetectable level. Moisture content in both of free cells and microencapsulated cells at 24° C significantly increased at week 2. While at 4°C, there was significant increase of moisture content observed in free cells at week 2, and microencapsulated cells showed significant increase of moisture content at week 10. Likewise, at -20°C, moisture content of both free and microencapsulated cells showed significant increase at week 2 and 10, respectively.