Lactation Performance Of Dairy Cows Research Articles

PSXIII-28 Late-Breaking: Lactational performance of dairy cows receiving supplemental His: A meta-analysis

Abstract The objective of this meta-analysis was to examine the effect of supplemental histidine (His) on dry matter intake (DMI), milk (MY) and energy-corrected milk (ECM) yields, and milk components in lactating dairy cows. A comprehensive meta-analysis was performed to data from 20 studies published in peer-reviewed journals between 1999 and 2021. In 12 of the studies His was supplemented to the diet as rumen-protected His and in the remaining experiments free His was infused into the abomasum (5 experiments), the jugular vein (2 experiments) or the duodenum (1 experiment) of the cows. The diets were based on corn silage in 60% and on grass silage in 40% of the studies. In studies where data were available (13 out of 20), metabolizable protein supply was estimated to be on average 98% (min = 85% and max = 110%) of NRC (2001) requirements. Computations were carried out using Comprehensive Meta-Analysis (V. 3.3.070). Histidine supplementation increased (P < 0.001) plasma His concentration by 59%, compared with control. Dry matter intake and MY increased (P ≤ 0.002) by 0.22 and 0.36 kg/d, respectively, whereas ECM yield tended to increase (P = 0.07) by 0.14 kg/d with His supplemented diets vs. the control. Milk protein concentration was also increased (P = 0.004) by 0.20 %-units, whereas milk fat concentration tended to decrease (P = 0.10) by 0.11 %-units with His supplementation. Further, His increased milk protein and lactose yield (P ≤ 0.05) by 0.31 and 0.14 kg/d, respectively. Overall, based on the available data, His supplementation of lactating dairy cow rations enhanced DMI, MY and milk protein concentration and yield.

Improvement of feed intake, digestibility, plasma metabolites, and lactation performance of dairy cows fed mixed silage of sugar beet pulp and rice straw inoculated with lactic acid bacteria

A study was conducted to investigate the inclusion effects of sugar beet pulp and rice straw mixture silage with inoculation (BRMS), in place of whole-plant corn silage (CS), on the dry matter intake, total-tract nutrient digestibility, plasma metabolites, rumen fermentation, and lactation performance in high-production dairy cows. Sixteen multiparous Holstein cows (body weight, 622 ± 35 kg; days in milk, 90 ± 11 d; mean ± standard deviation) were used in our experiments; the experiments were based on a repeated 4 × 4 Latin square design for 21 d, and each experimental period consisted of 14 d of adaptation, followed by 7 d of data collection. The 4 dietary treatments used were (dry matter basis): (1) 0% BRMS and 28.6% CS (0BRMS); (2) 4.3% BRMS and 24.3% CS (15BRMS); (3) 8.60% BRMS and 20.0% CS (30BRMS); and (4) 12.9% BRMS and 15.7% CS (45BRMS). The increasing inclusion of dietary BRMS was observed to linearly increase the total volatile fatty acids and the propionate concentration. The dry matter intake and digestibility values of neutral detergent fiber and acid detergent fiber increased linearly as the percentage of BRMS increased up to 45%. Milk yield linearly increased with the increase in the content of BRMS (39.0, 39.8, 40.9, and 40.3 kg/d for 0BRMS, 15BRMS, 30BRMS, and 45BRMS, respectively). The increasing inclusion of dietary BRMS induced a decrease in the ammonia nitrogen and milk urea nitrogen concentration, leading to a linear increase in milk protein production (1.15, 1.26, 1.35, and 1.27 kg/d for 0BRMS, 15BRMS, 30BRMS, and 45BRMS, respectively). In conclusion, the diets with the replacement of CS with BRMS up to 45% were beneficial to the production performance of high-production dairy cows, indicating that this method may be an appropriate use of sugar beet pulp and rice straw.

Multi-omics revealed the effects of rumen-protected methionine on the nutrient profile of milk in dairy cows

Cow’s milk is a highly-nutritious dairy product part of human diet worldwide. Rumen-protected methionine (RPM) is widely used to improve lactation performance of dairy cows, but understanding of the effects of RPM on milk nutrients composition are still limited. In this study, twenty mid-lactating dairy cows were supplemented with 20 gm/day RPM for 8 weeks to investigate the responses of milk nutritional composition to RPM. Metabolomics was applied for analyzing milk metabolites and 16S rRNA gene sequencing was used for analysis of rumen microbial composition. Milk fat content and yield were significantly increased after RPM supplementation. Totally 443 compounds belonging to 15 classes were identified, among which 15 metabolites were significantly changed. The functional nutrient α-ketoglutaric acid were significantly increased in the milk after RPM supplementation. We found 48 significantly differing bacterial genera in the rumen after supplementing RPM. Multi-omics integrated analysis revealed the higher abundance of Acetobacter, unclassified_f_Lachnospiraceae and Saccharofermentan contributed to the improved milk fat. In addition, the enriched abundance of Thermoactinomyces, Asteroleplasma, and Saccharofermentan showed positive correlations with higher α-ketoglutaric acid of milk. Our results uncover the metabolomic fingerprint and the key functional metabolites in the milk after supplementing RPM in dairy cows, as well as the key rumen bacteria associated with them. These findings provide novel insights into the development of functional dairy products that enriched the functional nutrient α-ketoglutaric acid or high milk fat.

Effects of harvest date and grass species on silage cell wall components and lactation performance of dairy cows

This study evaluated the effect of harvest date and forage species on the concentration of hydroxycinnamic acids in silage and its relationship to dairy cow performance. Tall fescue and timothy were harvested at a regular date on June 27 and July 8, respectively, or at a late date on July 8 and 25, respectively, in the first regrowth. Forage was treated with a salt-based additive and ensiled in hard-pressed round bales. Forty-seven lactating dairy cows were used in a block design. Cows received 1 of 4 treatments: (1) tall fescue harvested at regular date (RTF), (2) timothy harvested at regular date (RTI), (3) tall fescue harvested at late date (LTF), and (4) timothy harvested at late date (LTI). Diets were formulated to have the same forage-to-concentrate ratio (46:54 on a dry matter basis). Harvesting at late date increased fiber components, but only for timothy, where LTI contained greater neutral detergent fiber, acid detergent fiber, and acid detergent lignin concentrations than the other silages. Concentrations of hydroxycinnamic acids were affected by forage species, where concentrations of esterified ferulic acid and p-coumaric acid were greater for tall fescue silages than for timothy silages. Cows fed the RTI diet showed the greatest intakes of dry matter, organic matter, and crude protein. Feeding diets containing timothy silages increased milk yield and energy-corrected milk yield compared with tall fescue diets when averaged over harvest dates. Cows fed the RTI diet had greater milk protein yield than cows fed the RTF and LTF diets, and milk lactose yield was greater for cows fed diets containing timothy silage compared with tall fescue silage when averaged over harvest dates. Cows fed the LTF diet showed greater urinary N excretion compared with the LTI diet, but RTI showed the lowest urinary N and urea N excretions when calculated as percent of N intake. Cows fed diets containing timothy silage excreted more uric acid than cows fed tall fescue diets. Allantoin excretion was greater for cows eating the RTI and LTI diets compared with cows eating the RTF diet. Cows fed the RTI diet had a greater estimated microbial N flow and a greater excretion of hippuric acid than the RTF and LTF groups. In conclusion, besides the effect of harvest date on increasing the fiber components of timothy, concentrations of hydroxycinnamic acids were mainly affected by forage species; consequently, milk production was only affected by forage species. This indicates that hydroxycinnamic acids, such as ferulic acid, which cross-links to glucuronoarabinoxylans, was a major factor regulating milk production of cows fed tall fescue- and timothy silage-based diets, where lower hydroxycinnamic acid concentrations were responsible for greater milk yield.

Effect of substituting wet corn gluten feed and corn stover for alfalfa hay in total mixed ration silage on lactation performance in dairy cows

Wet corn gluten feed (WCGF) is a high moisture feed containing rapidly digestible, non-forage fiber and protein. The objective of this study was to investigate the effect of substituting WCGF and corn stover for alfalfa hay in total mixed ration (TMR) silage on lactation performance and nitrogen balance in dairy cows. Nine multiparous Holstein dairy cows (BW = 532 ± 28.9 kg and day in milk = 136 ± 5.6 d; mean ± SD) were used in a replicated 3 × 3 Latin square design with 21-d periods (14 d of diet adaption and 7 d of sample collection). Groups were balanced for parity, day in milk, and milk production and consumed one of three treatment diets during each period. The treatment diets were fed as TMR and contained similar concentrate mixtures and corn silage but different proportions of roughage and WCGF. The three treatments were: (1) 0% WCGF, 0% corn stover, and 22.1% alfalfa hay (0% WCGF); (2) 6.9% WCGF, 3.4% corn stover, and 11.8% alfalfa hay (7% WCGF); and (3) 13.3% WCGF, 4.9% corn stover, and 3.9% alfalfa hay (13.3% WCGF). Compared to the 0% WCGF diet, the cows fed the 7% and 13.3% WCGF diets had a higher milk yield and concentration of milk fat, protein, lactose, and total solids. Effective degradability of DM was higher in the cows fed the 7% and 13.3% WCGF diets than it was with the 0% WCGF diet. Cows fed the 13.3% WCGF had a higher CP effective degradability and a lower rumen undegraded protein than cows fed the 0% WCGF diet. The concentration of ruminal volatile fatty acids and ammonia-N was higher in cows fed the 7% and 13.3% WCGF diets than cows fed the 0% WCGF diet. The fecal N was lower in cows fed the 7% and 13.3% WCGF diets than it was in cows fed the 0% WCGF diet. Milk N secretion and milk N as a percent of N intake were higher in cows fed the 13.3% WCGF diet than cows fed the 0% and 7% WCGF diets. In conclusion, it appears that feeding a TMR silage containing WCGF and corn stover in combination, replacing a portion of alfalfa hay, may improve lactation performance and nitrogen utilization for lactating dairy cows.

Effects of the macroalga Asparagopsis taxiformis and oregano leaves on methane emission, rumen fermentation, and lactational performance of dairy cows

Asparagopsis taxiformis (AT) is a source of multiple halogenated compounds and, in a limited number of studies, has been shown to decrease enteric CH4 emission in vitro and in vivo. Similarly, oregano has been suggested as a potential CH4 mitigating agent. This study consisted of 2 in vitro and 2 in vivo experiments. Experiment (Exp.) 1 was aimed at establishing the effect of AT on CH4 emission in vitro. Two experiments (Exp. 2 and 3) with lactating dairy cows were conducted to determine the antimethanogenic effect of AT and oregano (Exp. 3) in vivo. Another experiment (Exp. 4) was designed to investigate stability of bromoform (CHBr3) in AT over time. In Exp. 3, 20 Holstein cows were used in a replicated 4 × 4 Latin square design with four 28-d periods. Treatments were basal diet (control) or basal diet supplemented with (dry matter basis) 0.25% AT (LowAT), 0.50% AT (HighAT), or 1.77% oregano (Origanum vulgare L.) leaves. Enteric gas emissions were measured using the GreenFeed system (C-Lock Inc., Rapid City, SD), and rumen samples were collected for fermentation analysis using the ororuminal technique. In Exp.1 (in vitro), relative to the control, AT (at 1% dry matter basis, inclusion rate) decreased CH4 yield by 98%. In Exp. 3, HighAT decreased average daily CH4 emission and CH4 yield by 65% and 55%, respectively, in experimental periods 1 and 2, but had no effect in periods 3 and 4. The differential response to AT among experimental periods was likely a result of a decrease in CHBr3 concentration in AT over time, as observed in Exp. 4 (up to 84% decrease in 4 mo of storage). In Exp. 3, H2 emission was increased by AT and, as expected, the proportion of acetate in the total volatile fatty acids in the rumen was decreased and those of propionate and butyrate were increased by HighAT compared with the control. Compared with the control, HighAT decreased dry matter intake, milk yield, and energy-corrected milk yield in Exp. 3. Milk composition was not affected by treatment, except lactose percentage and yield were decreased by HighAT. Concentrations of iodine and bromide in milk were increased by HighAT compared with the control. Milk CHBr3 concentration and its organoleptic characteristics were not different between control and HighAT. Oregano had no effect on CH4 emission or lactational performance of the cows in Exp. 3. Overall, AT included at 0.50% in the ration of dairy cows can have a large mitigation effect on enteric CH4 emission, but dry matter intake and milk production may also decrease. There was a marked decrease in the CH4 mitigation potential of AT in the second half of Exp. 3, likely resulting from CHBr3 decay over time.

Methionine precursor effects on lactation performance of dairy cows fed raw or heated soybeans

Two experiments were conducted to evaluate the effect of isopropyl ester of 2-hydroxy-4-(methylthio) butanoic acid (HMBi) on lactation performance of dairy cows. Experiment 1 evaluated the effect of HMBi in diets with 15.3% crude protein (CP) and with different proportions of rumen-degradable and undegradable protein. Variation in rumen-degradable and undegradable protein was achieved by replacing raw with heated soybeans. Experiment 2 was an on-farm trial to evaluate HMBi with a large number of observations and using a farm-formulated diet (17.2% CP). In experiment 1, 20 Holsteins at 100 ± 41 d in milk were allocated to 5 replicated 4 × 4 Latin squares with 21-d periods. Treatments were formed by a 2 × 2 factorial arrangement of raw or heated soybeans with or without HMBi. Paper capsules with HMBi were orally administered twice daily to each cow. Dosage of HMBi was 7.6 g of digestible Met/cow per day. There was no interaction between soybean type and HMBi. Heat-treated soybeans increased the yields of milk, protein, fat, and lactose, and reduced urea N in milk and plasma (PUN) compared with raw soybeans. Rumen microbial yield, dry matter intake (DMI), and the total-tract apparent digestibility of nutrients did not differ between soybean types. There was no evidence for HMBi-driven effects on DMI, milk and components yield, or diet digestibility. Urinary purine derivative excretion and PUN concentration were reduced in HMBi-fed cows compared with cows fed diets without HMBi. In experiment 2, 294 Holstein cows were blocked by parity and milk yield, and randomly assigned to HMBi (8.9 g of digestible Met/cow per day) or control. The final data set had 234 cows (215 ± 105 days in milk; 96 primiparous and 138 multiparous; 114 on control and 120 on HMBi) housed in 4 freestall groups (1 group/treatment per parity). The freestall group was the experimental unit for DMI, diet and orts composition, and feed availability. The HMBi supplement was top dressed for 28 d on the first daily meal of each cow, immediately after feed delivery of the same batch of feed to all 4 freestall groups (3 times per day). Sample collection and feed analysis occurred during the last 5 d. Spot urine samples and blood samples from each cow were obtained for analysis of the urinary allantoin to creatinine ratio and PUN. Feed availability, the contents of CP and neutral detergent fiber in diets and orts, and DMI did not differ. Cows fed with HMBi had greater milk protein yield and concentration compared with control and had no change in milk fat and lactose. Rumen microbial yield was greater and PUN was lower in HMBi-fed cows compared with control. In experiment 1, HMBi decreased rumen microbial yield and did not affect lactation performance, but it increased ruminal microbial yield and the secretion of milk protein in experiment 2. These results suggest that lactation response to HMBi may be partially mediated by ruminal events. Heated soybeans increased the efficiency of N utilization and the yields of milk, protein, fat, and lactose, but did not interact with HMBi supplementation.

Lactation Performance of Dairy Cows

Lactation Performance of Dairy Cows

Characterization of raft microdomains in bovine mammary tissue during lactation: How they are modulated by fatty acid treatments

The objective of the current study was first to characterize lipid raft microdomains isolated as detergent-resistant membranes (DRM) from mammary gland tissue, and second to determine how dietary fatty acids (FA) such as conjugated linoleic acid (CLA), 19:1 cyclo, and long-chain n-3 polyunsaturated FA affect lipid raft markers of mammary cells, and to finally establish relationships between these markers and lactation performance in dairy cows. Eight Holstein cows were used in a replicated 4 × 4 Latin square design with periods of 28 d. For the first 14 d, cows received daily an abomasal infusion of (1) 406 g of a saturated FA supplement (112 g of 16:0 + 230 g of 18:0) used as a control; (2) 36 g of a CLA supplement (13.9 g of trans-10,cis-12 18:2) + 370 g of saturated FA; (3) 7 g of Sterculia fetida oil (3.1 g of 19:1 cyclo, STO) + 399 g of saturated FA; or (4) 406 g of fish oil (55.2 g of cis-5,cis-8,cis-11,cis-14,cis-17 20:5 + 59.3 g of cis-4,cis-7,cis-10,cis-13,cis-16,cis-19 22:6, FO). Mammary biopsies were harvested on d 14 of each infusion period and were followed by a 14-d washout interval. Cholera toxin subunit B, which specifically binds to ganglioside M-1 (GM-1), a lipid raft marker, was used to assess its distribution in DRM. Infusions of CLA, STO, and FO were individually compared with the control, and significance was declared at P ≤ 0.05. Milk fat yield was decreased with CLA and FO, but was not affected by STO. Milk lactose yield was decreased with CLA and STO, but was not affected by FO. Mammary tissue shows a strong GM-1-signal enrichment in isolated DRM from mammary gland tissue. Caveolin (CAV) and flotillin (FLOT) are 2 proteins considered as lipid raft markers and they are present in DRM from mammary gland tissue. Distributions of GM-1, CAV-1, and FLOT-1 showed an effect of treatments determined by their subcellular distributions in sucrose gradient fractions. Regardless of treatments, data showed positive relationships between the yield of milk fat, protein, and lactose, and the abundance GM-1 in DRM fraction. Milk protein yield was positively correlated with relative proportion of FLOT-1 in the soluble fraction, whereas lactose yield was positively correlated with relative proportion of CAV-1 in the DRM fractions. Infusion of CLA decreased mRNA abundance of CAV-1, FLOT-1, and FLOT-2. Regardless of treatments, a positive relationship was observed between fat yield and mRNA abundance of FLOT-2. In conclusion, although limited to a few markers, results of the current experiment raised potential links between variation in specific biologically active component of raft microdomains in bovine mammary gland and lactation performances in dairy cows.

Feeding rumen-protected lysine prepartum increases energy-corrected milk and milk component yields in Holstein cows during early lactation

Feeding rumen-protected Lys (RPL) may be used to increase lactation performance in dairy cows; however, the effect of feeding RPL during the prepartum period and subsequent effect on postpartum performance is not well explored. Therefore, this experiment was conducted to determine the effects of feeding RPL (AjiPro-L Generation 3, Ajinomoto Heartland Inc., Chicago, IL) prepartum, postpartum, or both on performance, health, and blood metabolites. Seventy-five multiparous Holstein cows, blocked by parity, previous 305-d mature-equivalent milk production, expected calving date, and body condition score during the far-off dry period were assigned to 1 of 2 dietary treatments: total mixed ration with or without RPL in a randomized, complete block design. A 2 × 2 factorial arrangement of treatments was used. Prepartum (-28 d to calving), animals were fed a diet (forage, 68% of dietary DM) with RPL [PRE-L; 0.54% RPL of dietary dry matter intake (DMI)] or without RPL (control; PRE-C). After calving, half of the cows from each prepartum treatment group were assigned to a diet (forage, 55.5% of dietary DM) with RPL (PRE-L POST-L; PRE-C POST-L; 0.40% RPL of dietary DMI) or without RPL (PRE-C POST-C; PRE-L POST-C) until d 28 postpartum. Cows were milked twice a day and milk samples were taken on 7 ± 1.3, 14 ± 1.4, and 28 ± 1.1 d relative to calving (DRC). Milk yield and DMI were recorded daily. Blood samples were taken for plasma AA analysis on -7 ± 0.5, 0 ± 0.5, 7 ± 0.9, and 14 ± 0.9 DRC. Cows in PRE-L had greater body weight at -2 and -1 wk before calving compared with those in PRE-C, though body weight change from wk -4 to -1 was not different. Body weight (717 ± 6 kg) was greater and DMI (18.1 ± 0.7 kg) tended to be greater for cows in PRE-L POST-L and PRE-L POST-C compared with those that were in PRE-C POST-L and PRE-C POST-C (707 ± 6 and 16.8 ± 0.7 kg, respectively). Energy-corrected milk (48.8 ± 1.9 kg/d), milk fat (1.9 ± 0.1 kg/d), milk true protein (1.4 ± 0.1 kg/d), milk casein (0.6 ± 0.04 kg/d), and milk lactose yields (2.1 ± 0.1 kg/d) were greater for cows in PRE-L POST-L and PRE-L POST-C compared with those that were in PRE-C POST-L and PRE-C POST-C (44.2 ± 1.9, 1.7 ± 0.1, 1.3 ± 0.1, 0.5 ± 0.04, 1.9 ± 0.1 kg/d, respectively). Plasma concentrations of Lys prepartum (69.8 ± 1.8 µM) increased for cows in PRE-L compared with those in PRE-C (62.5 ± 1.3 µM). In conclusion, RPL consumed prepartum tended to increase postpartum DMI and increased energy-corrected milk and milk component yields. This indicates that prepartum supply of intestinally available Lys is pertinent to postpartum performance. However, postpartum supply of intestinally available Lys had no effect on cows' performance.

Impact of winter fodder beet or kale allocation on body condition score gain and early lactation performance of dairy cows

Forage crops such as fodder beet (FB) and kale are an important feature of dairy farming in Southland and Otago where winter pasture growth is negligible. However, farmers are concerned about poor performance of cows following winter FB feeding. In winter 2017, cows were offered FB or kale both with pasture baleage at two allocation rates: target (crop allocated to achieve a winter body condition score (BCS) gain of 0.7) or high (ad libitum crop). Diets with FB were lower in fibre, phosphorus, sulphur and calcium, but had a higher metabolizable energy, compared with kale diets. Body condition change and early lactation performance were monitored to compare effects of winter FB and kale diets. Average daily BCS gain before calving was similar for FB and kale cows. Crop type had a greater impact on cow performance than allocation rate. Cows wintered on FB had better reproductive performance (3-week pregnancy rate), and greater average milk solids, fat and protein yield (kg/d) than cows wintered on kale. Therefore, winter FB did not reduce cow performance compared with kale. However, the cumulative effects of a FB diet long term are yet to be determined and future research should monitor the impact on animal health.

Lactation performance, nitrogen utilization, and profitability in dairy cows fed fermented total mixed ration containing wet corn gluten feed and corn stover in combination replacing a portion of alfalfa hay

To effectively use corn by-products for ruminants, lactation performance, nitrogen utilization, and profitability in lactating dairy cows fed fermented total mixed ration (FTMR) that contained wet corn gluten feed (WCGF) and corn stover in combination replacing a portion of alfalfa hay were studied. Thirty-six multiparous Holstein dairy cows (602 ± 46.8 kg of body weight; 109 ± 18.5 day in milk; 30.1 ± 1.53 kg of milk production; and 2.44 ± 0. 51 of parity; mean ± SD) were blocked into 12 groups based on DIM, milk production, and parity and were allocated randomly to 1 of 3 diets for a 14-week period, with the first 2 weeks allowed for adaptation to the diets. The TMR was prepared with same mixed ration of concentrate mixtures and corn silage but different proportions of alfalfa hay, corn stover and WCGF. The treatments were designed as (1) TMR A containing 220 g/kg DM alfalfa hay; (2) TMR B containing 118 g/kg DM alfalfa hay, 68.8 g/kg DM WCGF, and 34.4 g/kg DM corn stover; and (3) Fermented TMR (FTMR) prepared with same ingredient as TMR B. The FTMR diet had higher crude protein (CP) and lactic acid contents content, the ratio of ammonia nitrogen to total N while decreased pH value, neutral detergent fiber (NDF), acid detergent fiber (ADF) and dry matter (DM) content compared to other two diets. The milk fat concentration, milk fat yield, DM and CP degradation, estimated microbial CP yield and feed efficiency were higher (P < 0.05) in the cows fed FTMR treatment but DM intake was lower (P < 0.05) in the cows fed the FTMR treatment than the cows fed the other treatments. The concentrations of rumen volatile fatty acids, except for butyrate, were highest (P < 0.0001) in cows fed the FTMR diet followed by cows fed TMR B and TMR A diets. The cows fed the FTMR diet had a higher molar proportion of acetate, total-tract apparent digestibility and ammonia-N concentration and a lower molar proportion of butyrate, organic matter, NDF, and ADF intakes than the cows fed the other diets. The cows fed the FTMR diet decreased fecal N excretion and retention N, increased the urea nitrogen concentration in the urine and milk and improved income over feed cost per cow per day compared with the cows fed the other diets. The results confirmed that FTMR prepared with WCGF and corn stover in combination replacing a portion of alfalfa hay is a suitable choice for improving lactation performance of dairy cows.

Effects of a high-protein corn product compared with soy and canola protein sources on nutrient digestibility and production responses in mid-lactation dairy cows

An experiment was conducted to assess the effects of a novel and proprietary high-protein corn product [56% crude protein (CP)] relative to other common sources of protein on the lactation performance of dairy cows. Twenty-four Holstein cows (620 ± 47.7 kg of body weight, 111 ± 34 d in milk, 2.28 ± 0.46 lactations; mean ± standard deviation) were randomly assigned to treatment sequence in a replicated 4 × 4 Latin square design balanced for carryover effects. Cows were individually fed 1 of 4 diets with a different protein concentrate source during each 28-d period, including soybean meal (SBM), high-protein corn product (HPCP), soybean meal with rumen-bypass soy protein (SBMBP), and canola meal with rumen-bypass soy protein (CANBP). Diets were formulated for equal concentrations of CP and balanced to meet predicted lysine and methionine requirements. The SBM diet was formulated to provide 5.7% rumen-undegradable protein (RUP), whereas SBMBP and CANBP diets were formulated for 6.8% RUP to match HPCP. Data were analyzed using mixed models with the fixed effects of treatment, period, square, the interactions of treatment and period and of treatment and square, and the random effect of cow. The CANBP diet increased dry matter intake (DMI) compared with SBM and HPCP. Treatment affected milk yield, as SBMBP and CANBP increased yield compared with SBM, but HPCP decreased milk yield compared with all treatments. The HPCP diet reduced CP intake as a percent of total DMI and increased the CP content of orts, indicative of selection against HPCP. The HPCP diet also decreased apparent total-tract and CP digestibility, leading to less urine nitrogen excretion and greater fecal nitrogen output. The SBMBP and CANBP diets performed similarly in nearly every variable measured, except that SBMBP increased milk urea nitrogen. In conclusion, the HPCP diet reduced yield of milk and milk components, likely because of reduced apparent total-tract dry matter and CP digestibility.

Lactation performance and nitrogen utilization of dairy cows on diets including unfermented or fermented yellow wine lees mix

Yellow wine lees (YWL) consist of a high content of crude protein with relatively low price. With microbial pretreatment, the increased crude protein content in the fermented YWL mix may be beneficial for the lactation performance of dairy cows. The current study was conducted to investigate lactation performance and nitrogen (N) utilization in lactating dairy cows receiving unfermented and fermented YWL mix as alternative protein source for soybean meal. Fifteen multiparous mid-lactation Chinese Holstein dairy cows with similar parity (2.30, SD 0.32), days-in-milk (190, SD 15.2) and milk yield (25.0, SD 0.45 kg) were used in a replicated 3 × 3 Latin square design. Diets were isonitrogenous and isocaloric, with a forage-to-concentrate ratio of 60:40 [dry matter basis]. Three dietary treatments were designed as follows (dry matter basis): (1) total mixed ration containing 18% soybean meal (Control), (2) total mixed ration containing 11% unfermented YWL mix (UM), and (3) total mixed ration containing 11% fermented YWL mix (FM). Each period lasted for 20 d, with the first 15 d used for adaptation and the next 5 d used for sampling. Dry matter intake (P = 0.04), milk yield (P = 0.02), milk protein yield (P = 0.02) and energy-corrected milk yield (P = 0.05) were higher in cows fed the control and FM diets than in those cows fed the UM diet. Milk composition, feed efficiency (milk yield/dry matter intake), and N conversion rate were not different (P > 0.05) among the three groups. The cows fed the UM diet showed lower N intake (P < 0.01), blood urea N (P = 0.02), urinary N (P = 0.04), microbial protein (P = 0.04) and metabolizable protein (P < 0.01) than the cows fed the control or FM diets. Feeding of the FM-fed cows tended to have greater IOFC than that of the control (P = 0.08), In summary, both unfermented and fermented YWL can be used as a protein source feed in the diets for lactating cows and inclusion of the fermented YWL had no adverse effects on lactation performance and N utilization but with greater profit.

Effects of Dietary Rumen-Protected Betaine on Lactation Performance and Serum Metabolites of Mid-lactation Holstein Dairy Cows

This experiment was conducted to investigate the effects of dietary rumen-protected betaine (RPB) supplementation, as partial replacement for methionine, on the lactation performance of mid-lactation dairy cows. A total of 36 Holstein dairy cows were randomly assigned to three groups [control, 20 g/day RPB, or 15 g/day rumen-protected methionine (RPM)]. The experiment was conducted over 9 weeks, with the first week for adaptation. Blood metabolites were analyzed with metabolomics in the control and RPB groups. The results revealed that the milk yield and milk protein content were higher in cows fed RPB and RPM compared to those in the control group. Concentrations of nine metabolites differed between cows in the RPB and control groups. These metabolites were mainly concentrated in six pathways, such as arginine synthesis and proline degradation and cyanoamino acid synthesis. This study revealed that RPB can spare methionine and improve lactation performance of dairy cows fed with diets moderately deficient in methionine.

Timing of initiation and duration of feeding rumen-protected choline affects performance of lactating Holstein cows

Objectives were to evaluate the effects of altering timing of initiating and duration of supplementing rumen-protected choline (RPC) on lactation performance in dairy cows. The hypothesis was that RPC increases yields of milk and milk components, regardless of when supplementation is initiated, and that the effects of supplementing RPC starting prepartum and continuing post-transition would be additive. Cows at 241 ± 2.2 d of gestation were blocked by parity group (49 entering lactation 2, 50 entering lactation >2) and 305-d milk yield and, within block, assigned randomly to 1 of 4 treatments arranged as a 2 × 2 factorial with 2 levels of choline in transition, from 21 d pre- to 21 d postpartum, and 2 levels of choline in post-transition, from 22 to 105 d postpartum. The 2 levels of RPC supplemented were either 0 g/d or 12.9 g/d of choline ion fed as 60 g/d of an RPC product that was top-dressed onto the total mixed ration. Thus, treatments were as follows: NN (n = 25): no choline in transition or post-transition; NC (n = 25): no choline in transition and choline in post-transition; CN (n = 25): choline in transition and no choline in post-transition; CC (n = 24): choline in transition and in post-transition. Prepartum, treatments were supplemented (mean ± SD) for the last 18.8 ± 5.7 and 19.2 ± 5.0 d of gestation in treatments with 0 or 12.9 g/d of choline ion, respectively. Supplementing RPC prepartum did not affect dry matter intake (DMI), body weight (BW), or body condition score (BCS) in the last 3 weeks of gestation. Likewise, RPC did not affect the yield or the composition of colostrum. Supplementation with RPC during transition increased fat percent by 0.02 percentage units, fat yield by 0.16 kg/d, and energy-corrected milk (ECM) by 3.1 kg/d in the first 21 d postpartum, and increased fat yield by 0.10 kg/d and ECM by 2.4 kg/d from 22 to 105 d postpartum. Supplementing RPC during transition did not affect DMI postpartum, but it improved feed efficiency, and cows produced 0.11 kg/d more ECM per kg of DMI. Changes in BW and BCS during the first 21 d postpartum did not differ between treatments. Cows fed RPC during transition had more negative net energy balance and 0.1 unit smaller BCS in the first 105 d postpartum than non-supplemented cows. Supplementing RPC in post-transition did not influence productive performance in dairy cows, and choline supplementation during transition or post-transition did not affect measures of reproduction. Collectively, supplementing RPC to supply 12.9 g/d of choline ion benefited productive performance in dairy cows when supplementation occurred during the transition period, but no additional benefit was observed from supplementing RPC past 22 d postpartum.

Impact of heat stress on lactational performance of dairy cows

Lactating dairy cows exhibit a myriad of responses to heat stress. These responses partially facilitate the thermal balance between heat gain and heat loss, but also account for reduction in productivity. Decreased milk yield is the most recognized impact of heat stress on a dairy cow and results in significant economic loss to dairy producers. The reduced milk yield by heat stress is observed when daily average temperature-humidity index exceeds 68, above which the milk yield of a cow is negatively correlated with temperature-humidity index or dry bulb temperature. Milk yield is also positively correlated with body temperature of the cows under evaporative cooling, which reflects the positive relationship between metabolic heat production and milk yield. During summer, feed intake is positively correlated with milk yield, and the decreased intake explains at least half of the reduction in milk yield by heat stress. These emphasize the importance of maintaining intake on productivity during summer. Although not entirely clear, mechanisms that mediate the reduced milk yield by heat stress in addition to intake may be multifactorial. These could include but are not limited to altered metabolism, potential activation of immune system and inflammation, changes in behavior, and altered mammary gland development and function.

Effect of extrusion on the chemical composition of the faba beans and its influence on lactation performance of dairy cows

Effect of extrusion on the chemical composition of the faba beans and its influence on lactation performance of dairy cows

Evaluation of Extruded Finger Millet Straw Based Complete Diet in Lactating Dairy Cows

A study was undertaken to evaluate the effect of feeding finger millet straw based extruded complete feed on the lactational performance of dairy cows. Six crossbred cows in late lactation were divided into two groups of three each and used in a switch over design. Treatment diets included control group (CON) fed compounded feed mixture and finger millet straw as a roughage source offered separately and ECF group fed with the same diet in the form of extruded complete feed. Digestibility (%) of DM, OM and NDF was higher in the ECF compared to the CON diet (P<0.05). The total milk (5.34 vs. 5.97 kg/d) and 4% FCM (5.63 vs. 6.66 kg/d) were significantly higher (P<0.05) in ECF compared to CON group. Considering the milk constituents (per cent) of CON and ECF groups, the fat (4.37 and 4.71%), SNF (9.02 and 9.23%), protein (3.31 and 3.39%) and lactose (4.94 and 5.06%) contents were significantly higher in ECF group (P<0.05). The efficiency of net energy (NE) utilisation for milk production was higher with feeding of extruded complete feed, with more NE diverted to milk production than the cows of control group. Considering the overall results of the study, it is concluded that feeding of finger millet straw based extruded complete feed could improve the lactational performance of cows in late lactation possibly leading to the increased efficiency of feeding management in dairy production.

Effects of sodium selenite and coated sodium selenite on lactation performance, total tract nutrient digestion and rumen fermentation in Holstein dairy cows

Se can enhance lactation performance by improving nutrient utilization and antioxidant status. However, sodium selenite (SS) can be reduced to non-absorbable elemental Se in the rumen, thereby reducing the intestinal availability of Se. The study investigated the impacts of SS and coated SS (CSS) supplementation on lactation performance, nutrient digestibility, ruminal fermentation and microbiota in dairy cows. Sixty multiparous Holstein dairy cows were blocked by parity, daily milk yield and days in milk and randomly assigned to five treatments: control, SS addition (0.3 mg Se/kg DM as SS addition) or CSS addition (0.1, 0.2 and 0.3 mg Se/kg DM as CSS addition for low CSS (LCSS), medium CSS (MCSS) and high CSS (HCSS), respectively). Experiment period was 110 days with 20 days of adaptation and 90 days of sample collection. Dry matter intake was higher for MCSS and HCSS compared with control. Yields of milk, milk fat and milk protein and feed efficiency were higher for MCSS and HCSS than for control, SS and LCSS. Digestibility of DM and organic matter was highest for CSS addition, followed by SS addition and then control. Digestibility of CP was higher for MCSS and HCSS than for control, SS and LCSS. Higher digestibility of ether extract, NDF and ADF was observed for SS or CSS addition. Ruminal pH decreased with dietary Se addition. Acetate to propionate ratio and ammonia N were lower, and total volatile fatty acids (VFAs) concentration was greater for SS, MCSS and HCSS than control. Ruminal H ion concentration was highest for MCSS and HCSS and lowest for control. Activities of cellobiase, carboxymethyl-cellulase, xylanase and protease and copies of total bacteria, fungi, Ruminococcus flavefaciens, Fibrobacter succinogenes and Ruminococcus amylophilus increased with SS or CSS addition. Activity of α-amylase, copies of protozoa, Ruminococcus albus and Butyrivibrio fibrisolvens and serum glucose, total protein, albumin and glutathione peroxidase were higher for SS, MCSS and HCSS than for control and LCSS. Dietary SS or CSS supplementation elevated blood Se concentration and total antioxidant capacity activity. The data implied that milk yield was elevated due to the increase in total tract nutrient digestibility, total VFA concentration and microorganism population with 0.2 or 0.3 mg Se/kg DM from CSS supplementation in dairy cows. Compared with SS, HCSS addition was more efficient in promoting lactation performance of dairy cows.