Laboratory Conditions Research Articles

Effect of Transient Spark Discharge and Plasma Activated Water Treatments against Fusarium graminearum Infected Wheat Grains under Laboratory Conditions

AbstractOver the last decade, more and more attention has been paid to applications of non-thermal plasma in agriculture, where it is used to decontaminate various microorganisms and to improve the seed germination. In this study, we present the results of a newly developed point-to-ring NTP transient spark discharge apparatus (NTP), plasma activated water (PAW) and their combined treatment on Durum wheat and Common wheat grains under laboratory conditions. Transient spark discharge treatment was used as direct treatment while indirect treatment of wheat grains was performed by PAW produced in point-to-plane NTP transient spark apparatus. We found that the degree of grain surface decontamination was in order NTP > PAW > combined treatment. In the case of Durum wheat grain germination, all treatments increased germination with increasing exposure times, while in the case of Common wheat, PAW treatment and combined treatment did not significantly increase the grain germination. In conclusion, plasma treatment has enormous potential for use in agriculture and its possibilities need to be fully explored.

Alternative methodology of special coffee roasting in the electric popcorn maker

The aim was to develop and evaluate an alternative method for roasting coffee, using a domestic electric popcorn machine, estimating an ideal roasting time to obtain a drink of high quality. The experiment was conducted under laboratory conditions, with 10 treatments, with roasting times ranging from 3 to 8 minutes. Grain mass reduction and roasting color classification, according to the ABG Agtron scale, were evaluated. Roasting was carried out in a domestic electric popcorn maker, from Mondial brand, model PP-04, with a power of 1200W. Time intervals of five to six minutes showed better coffee characteristics, medium roasting point, ideal for specialty coffees. The use of the popcorn machine as a coffee roaster showed a satisfactory result, since in a short period of time, it provided a uniform roast, which attributes superior quality, compared to conventional coffee found on the market.

Texture prediction of natural soils in the Brazilian Amazon through proximal sensors

Proximal sensors provide fast, low-cost, environmentally friendly, and reliable analyses for the characterization of soils and other materials. Numerous studies have been conducted on soils in temperate regions, but there are knowledge gaps regarding the use of these devices in tropical soils, especially in the Amazon region. In this regard, this study utilized portable proximal sensors of X-ray fluorescence spectroscopy (pXRF) and diffuse reflectance spectroscopy in the visible to near-infrared region (Vis-NIR) for predicting the texture of natural soils in 61 municipalities in the state of Pará, Amazon region, Brazil. The objectives were: i) to investigate the accuracy of soil texture prediction based on data from sensors separately and sensor fusion (pXRF and Vis-NIR data) using two supervised algorithms (Random Forest, RF, and Support Vector Machine, SVM) and ii) to assess the effect of soil horizon (superficial and subsuperficial horizons, and their combination) in predicting the texture of tropical natural soils. In total, 233 soil samples were collected in the 0–20 cm and 80–100 cm depths, equivalent to superficial and subsuperficial horizons in areas with primary or secondary forest cover with at least 20 years of natural regeneration and approximately 20 ha of coverage area. The hydrometer method was used for soil texture analysis. In parallel, a portion of the soil samples was analyzed by pXRF and Vis-NIR, in triplicate, under laboratory conditions. The predictive models with RF were more robust compared to the models obtained with SVM, according to ratio performance interquartile distance (RPIQ), coefficient of determination (R2), root mean square error (RMSE), and mean absolute error (MAE). The R2 values obtained by pXRF, Vis-NIR, and sensor data combination were, respectively, 0.89, 0.87, and 0.93 for sand; 0.92, 0.90, and 0.93 for clay; and 0.91, 0.67, and 0.93 for silt. Overall, clay prediction models achieved higher R2 values compared to sand and silt models. Soil texture prediction using sensor fusion showed lower RMSE values and higher R2 and RPIQ values, respectively (sand: 7.79, 0.93, 4.69; clay: 5.58, 0.93, 3.86; and silt: 5.72, 0.92, 2.92) compared to the best-performing sensor individually (Vis-NIR). With regard to the optimal model utilizing individual sensor data, Vis-NIR models exhibited reduced error for clay and sand prediction. The effect of combining horizons to a single and bigger dataset was minimally important for the models. The results demonstrate confidence in the use of proximal sensors for soil texture assessment in natural Amazon soils, aiming to reduce costs and the time required for analyses.

Compound-Specific Isotope Analysis Provides Direct Evidence for Identifying the Source of Residual Pesticides Diazinon and Procymidone in the Soil-Plant System.

Compound-specific isotope analysis stands as a promising tool for unveiling the behavior of pesticides in agricultural environments. Using the commercial formulations of persistent fungicide procymidone (PRO) and less persistent insecticide diazinon (DIA), respectively, we analyzed the concentration and carbon isotope composition (δ13C) of the residual pesticides through soil incubation experiments in a greenhouse (for 150 days) and lab conditions (for 50-70 days). Our results showed that the magnitude of δ13C variation depends on pesticide specificity, in which PRO in the soil exhibited little variation in δ13C values over the entire incubation times, while DIA demonstrated an increased δ13C value, with the extent of δ13C variability affected by different spiking concentrations, plant presence, and light conditions. Moreover, the pesticides extracted from soils were isotopically overlapped with those from crop lettuce. Ultimately, the isotope composition of pesticides could infer the degradation and translocation processes and might contribute to identifying the source(s) of pesticide formulation in agricultural fields.

Detection of fucosylated extracellular vesicles miR-4732-5p related to diagnosis of early lung adenocarcinoma by the electrochemical biosensor

Our preliminary investigation has identified the potential of serum fucosylated extracellular vesicles (EVs) miR-4732-5p in the early diagnosis of lung adenocarcinoma (LUAD) by a fucose-captured strategy utilizing lentil lectin (LCA)-magnetic beads and subsequent screening of high throughput sequencing and validation of real-time quantitative polymerase chain reaction (RT-qPCR). Considering the relatively complicated procedure, expensive equipment, and stringent laboratory condition, we have constructed an electrochemical biosensor assay for the detection of miR-4732-5p. miR-4732-5p is extremely low in serum, down to the fM level, so it needs to be detected by highly sensitive electrochemical methods based on the Mg2+-dependent DNAzyme splitting nucleic acid lock (NAL) cycle and hybridization chain reaction (HCR) signal amplification. In this study, signal amplification is achieved through the dual amplification reactions using NAL cycle in combination with HCR. In addition, hybridized DNA strands bind to a large number of methylene blue (MB) molecules to enhance signaling. Based on the above strategy, we further enhance our signal amplification strategies to improve detection sensitivity and accuracy. The implementation of this assay proceeded as follows: initially, miR-4732-5p was combined with NAL, and then Mg2+-dependent DNAzyme splitted NAL to release auxiliary DNA (S1) strands, which were subsequently captured by the immobilized capture probe DNA (C1) strands on the electrode surface. Following this, abundant quantities of DNA1 (H1) and DNA2 (H2) tandems were generated by HCR, and S1 strands then hybridized with the H1 and H2 tandems through base complementary pairing. Finally, MB was bonded to the H1 and H2 tandems through π–π stacking interaction, leading to the generation of a signal current upon the detection of a potential capable of inducing a redox change of MB by the electrode. Furthermore, we evaluated the performance of our developed electrochemical biosensor assay. The results demonstrated that our assay is a reliable approach, characterized by its high sensitivity (with a detection limit of 2.6 × 10−17 M), excellent specificity, good accuracy, reproducibility, and stability. Additionally, it is cost-effective, requires simple operation, and is portable, making it suitable for the detection of serum fucosylated extracellular vesicles miR-4732-5p. Ultimately, this development has the potential to enhance the diagnostic efficiency for patients with early-stage LUAD.

Impact of copper and zinc oral chronic exposure on Carniolan honey bee survival and feeding preference.

Honey bees are important plant pollinators and honey producers. Contamination of the environment with metals can lead to a decline in honey bee populations. Copper (Cu) and zinc (Zn) salts are commonly used as fungicides and foliar fertilizers. In this study, we investigated the effects of 10-day chronic oral exposure to different concentrations of Cu (CuSO4) and Zn (ZnCl2) on survival and feeding rates of Carniolan honey bees in laboratory conditions. We found that mortality in honey bee workers increased in a concentration-dependent manner and that Cu (lethal concentration [LC50] = 66mg/l) was more toxic than Zn (LC50 = 144mg/l). There was no difference in the feeding rate of Cu-treated bees for the different concentrations tested, but the feeding rate decreased with the increase in Zn concentration. To determine feeding preference or avoidance for Cu and Zn, we conducted 2-choice 24-h feeding experiments. We demonstrated that honey bees preferred Zn-containing solutions compared to the control diet. A two-choice experiment with Cu showed a tendency for honey bees to be deterred by Cu at high concentrations; however, it was not statistically significant. In summary, our results suggest that honey bee workers may suffer adverse effects when exposed to ecologically relevant concentrations of Cu and Zn.

Bioconcentration capacity of moss Leskea angustata Tayl., for heavy metals and its application in the atmospheric biomonitoring of a metropolitan area

In this work, the bioconcentration capacity of heavy metals Cd, Cu, Pb and Zn in the moss Leskea angustata Tayl., exposed under laboratory conditions was evaluated, and subsequently the usefulness of moss for the heavy metals' biomonitoring associated with suspended particulate matter in air of the Toluca Valley Metropolitan Area was determined by means of the moss bag technique. To achieve the aims, bioassays were carried out in the laboratory with the moss, the exposure for a period of 6 months of the moss bags in the sites of the Automatic Atmospheric Monitoring Network of the Government of the State of Mexico and their subsequent analysis through of elemental and structural characterization techniques. The results of the bioassays on the moss L. angustata, demonstrate that this moss has adequate bioconcentration capabilities to be used as a biomonitor of heavy metals in polluted areas. The structural characterization of the moss L. angustata, shows that this moss has good adsorption characteristics (presence of functional groups, surface area of 19.87 m2 g−1, average pore diameter equal to 2.86 nm and a total pore volume of 0.014218 cm3 g−1). The significant correlations between Cd–Zn–Pb and between Pb and Zn with PM2.5, as well as a strong correlation between Pb and Zn with PM10, allow us to establish that these three metals have their main origin in the domestic combustion of wood and liquid petroleum gas (LP gas), as well as emissions from agricultural burning in the study area. Cu has a different origin than other metals and PM10 and PM2.5. The findings demonstrate a strong association between anthropogenic activities - such as domestic wood and LP gas combustion, agricultural burning emissions, vehicular emissions, tire wear, and industrial activities - and the detection, entrapment, and accumulation of several heavy metals by L. angustata moss.

Evaluation of the applicability of carbon-based materials for arsenic removal from flue gases

Arsenic is an highly toxic element which may be released into the atmosphere during the combustion of solid fuels. This paper verifies the possibility of using char from tires CT and lignite dust LD as materials for the adsorption of gaseous forms of As. The source of the gaseous forms of arsenic was coal combustion under laboratory conditions. Adsorption tests included both different sorbent bed temperatures (120–180°C) and various flue gas compositions (resulting from the combustion of bituminous and lignite coals). It was shown that sorption temperature did not affect the arsenic removal efficiency for both analysed sorbents. The average As removal efficiency for tire char and lignite dust was 88 and 92%, respectively. The composition of the burned coal, and thus the composition of the flue gas, significantly influences the efficiency of Asgas adsorption. While for LD the level of As removal was still high (more the 90%), the sorption of Asgas by tire char from the flue gas when lignite was burned was less effective (the efficiency was below 80%). It was additionally indicated via a leachability test that "used" CT could be safely disposed of while LD would require appropriate management. Based on the comparison of the performance of CT and LD with commercial AC activated carbon and the differences in their market price, it can be concluded that the studied alternative sorbents are competitive with AC.

Исследование физико-механических свойств ийолит-уртита в условиях одноосного и трехосного сжатия

The study of deformation and failure processes in rock masses is often carried out in laboratory conditions on samples. Widely used test equipment is used for this purpose, which makes it possible to create loading conditions similar to those inside the rock mass. Basically, the loading mode is implemented when forces are applied only to one side of the sample (along one axis), that is, uniaxial compression or tension. Test equipment that allows creation of loading along three axes, i.e. triaxial compression, is used less frequently. Such equipment is often very expensive, and testing requires a lot of time and resources. Nevertheless, this loading mode is one of the most interesting, since it is close to real conditions in the rock mass. This paper presents the results of experimental studies of ijolite-urtite samples (rocks from the Khibiny massif, the Kola Region) in conditions of uniaxial and triaxial compression. The purpose of the research is to establish how the values of the compressive strength of this rock change, to assess the critical values of the specific strain energy, as well as the tendency to dynamic destruction during the transition from the uniaxial loading mode to the triaxial one. The experimental studies revealed that there exists a sharper increase in the values of compressive strength and the critical values of specific strain energy of the ijolite-urtite samples in conditions of triaxial compression than under uniaxial compression. It has been established that the studied rock is prone to dynamic failure under uniaxial compression and preserve its tendency to this type of failure under triaxial compression.

Synthesis, Characterization, Molecular Docking, and Investigation of Antibacterial Properties of New Derivatives of 1-H-Phenanthro [9,10-d] Imidazole.

In this study, several imidazole derivatives in one pot multicomponent reaction from various aldehydes 1(a-z), 9,10-phenanthrenequinone, or benzyl (2), and ammonium acetate (3) were synthesized in the presence of acetic acid (AcOH) under reflux conditions at 120 °C. Also, the photochromic properties of synthesized compounds were investigated in AcOH as a solvent under laboratory conditions at a temperature of 120 °C. Moreover, the antibacterial activity of the synthesized compounds was investigated. The structure of the products was confirmed using FT-IR, UV-Vis, 1H-NMR, and 13CNMR spectroscopy. The antimicrobial activity of these compounds against gram-positive bacteria including Bacillus subtilis (B. subtilis) and gram-negative bacteria including Escherichia coli (E.coli) bacteria was evaluated by the Well diffusion (WD) method, and the compounds 4 o showed significant results for both antibacterial activity. To gain insight into how these compounds interact with two types of targets, i. e., human topoisomerase II alpha (5GWK) and acetylcholinesterase (7AIX), binding calculations have been used that provide significant results for both targets and show that most ligands can effectively bind to cleft nucleotides. Interfere in the first one or be well placed in them. Hydrophobic pocket in the dimension, which can ultimately lead to high scores achieved.

PHYSICAL FACTORS OF THE INFLUENCE THE EXPLOSION ON ENCLOSURE GLASS STRUCTURES. OVERVIEW OF TESTING METHODS

The article examines the main physical parameters of an explosive wave, the properties of explosive loads, as well as the peculiarities of the propagation of waves in space and their dynamic influence on enclosing structures, including translucent The considered temporal and spatial conditions under which the formation of the quasi-flat front of the blast wave takes place and the blast loads can be considered as uniformly distributed along the vertical plane. If the distance to the explosive charge exceeds half of the value of the width and height of the structure or its element (provided that the charge is located in the center of the structure or element), then the load on them can be considered approximately averaged by area. The thermophysical factors of influence, which the building structure must withstand, are analyzed. The main differences of explosive and shock effects from static loads, which are taken into account during construction design, are described. The main real-time methods of determining the parameters of the blast wave in real time and the factors of its destructive effect on the elements of the enclosing structures of buildings are considered. The determination of the critical pressure for a specific structure is tied to the determination of the critical distance at which the structure will not withstand the destructive impact of the shock wave. The main real-time methods of determining the blast wave parameters in real time are considered. It was determined that the use of the shock tube method is rational for conducting research in laboratory conditions. The work was carried out with the assistance of the National Research Fund of Ukraine. The materials of the article contain scientific results obtained as part of the implementation of the project No. 208/0172 "Aerodynamics, heat exchange and innovations to increase the energy efficiency of window structures and their use for the reconstruction of war-damaged buildings of Ukraine" of the competition "Science for the reconstruction of Ukraine in the war and post-war periods".

An essential component of antimicrobial stewardship during the COVID-19 pandemic in the intensive care unit: de-escalation

BackgroundThe antimicrobial de-escalation strategy (ADE) plays a crucial role in antimicrobial stewardship, reducing the likelihood of bacterial resistance. This study aims to evaluate how often the intensive care unit (ICU)...

Toxicity of Bt Protein on Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) Fed With Corn Leaves in Paraguay

The fall armyworm Spodoptera frugiperda is the most destructive pest in corn cultivation in Paraguay with yield losses of 20 to 70%. The adoption of genetically modified crops (GMO) is a valid technique for managing this pest. The objective of this investigation was to evaluate the toxicity among Bt events used locally; determining mortality and important biological parameters. The S. frugiperda population previously collected from the field was raised under laboratory conditions fed with leaves from events MIR162TM (Vip3A19), VT3ProTM (Cry1A105, Cry2Ab2, Cry3Bb), PowerCoreTM (Cry1A105, Cry1F, Cry2Ab2) and a treatment with non-maize. Bt BR106TM. The mortality of 120 larvae was evaluated for each treatment and the viability until the adult stage of those larvae that managed to survive the different treatments was observed. A high toxicity effect was verified with 100% mortality of neonatal larvae fed with the MIR162TM (Vip3A19) event; as for the other events, 86% and 61% VT3ProTM (Cry1A105, Cry2Ab2, Cry3Bb), and PowerCoreTM (Cry1A105, Cry1F, Cry2Ab2) respectively; with 13% mortality for the non-Bt control variety BR106TM. The oviposition period, viability and number of eggs laid in adults surviving the PowerCoreTM (Cry1A105, Cry1F, Cry2Ab2) and VT3ProTM (Cry1A105, Cry2Ab2, Cry3Bb) events were reduced, affecting the fecundity of the surviving insect population.

Toxicity of Bt Protein on Spodoptera frugiperda (Smith) (Lepidoptera: Noctuidae) Fed With Corn Leaves in Paraguay

The fall armyworm Spodoptera frugiperda is the most destructive pest in corn cultivation in Paraguay with yield losses of 20 to 70%. The adoption of genetically modified crops (GMO) is a valid technique for managing this pest. The objective of this investigation was to evaluate the toxicity among Bt events used locally; determining mortality and important biological parameters. The S. frugiperda population previously collected from the field was raised under laboratory conditions fed with leaves from events MIR162TM (Vip3A19), VT3ProTM (Cry1A105, Cry2Ab2, Cry3Bb), PowerCoreTM (Cry1A105, Cry1F, Cry2Ab2) and a treatment with non-maize. Bt BR106TM. The mortality of 120 larvae was evaluated for each treatment and the viability until the adult stage of those larvae that managed to survive the different treatments was observed. A high toxicity effect was verified with 100% mortality of neonatal larvae fed with the MIR162TM (Vip3A19) event; as for the other events, 86% and 61% VT3ProTM (Cry1A105, Cry2Ab2, Cry3Bb), and PowerCoreTM (Cry1A105, Cry1F, Cry2Ab2) respectively; with 13% mortality for the non-Bt control variety BR106TM. The oviposition period, viability and number of eggs laid in adults surviving the PowerCoreTM (Cry1A105, Cry1F, Cry2Ab2) and VT3ProTM (Cry1A105, Cry2Ab2, Cry3Bb) events were reduced, affecting the fecundity of the surviving insect population.

EFFECT OF VARIOUS CHEMICAL TREATMENTS ON PHYSICOCHEMICAL AND THERMAL PROPERTIES OF ERYTHRINA VARIEGATA FIBERS: APPLICATION IN EPOXY COMPOSITES

Recently, there has been an increasing trend in utilizing lignocellulosic fiber reinforced composites in structural applications within the construction and automobile industries, replacing conventional materials based on metals and their derivatives. In the present study, Erythrina variegata fibers (EVFs) were subjected to a number of chemical treatments individually (alkalization, benzoyl peroxide, potassium permanganate, and stearic acid treatments). The effects of these chemical treatments on the EVFs were examined through chemical composition analysis, X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), and thermogravimetric analysis (TGA). This comprehensive analysis aimed to assess the suitability of the chemically treated EVFs for use as reinforcement in thermoset polymer matrix composites. The alkali treated fibers (AEVFs) were found as optimum and were then used as reinforcement in epoxy adhesives. Different fiber loadings (0, 5, 10, 15, 20, and 25 wt%) were incorporated into the epoxy matrix to investigate their effects on the properties of the composites. Therefore, the tensile strength, flexural strength, impact strength, and thermal stability of the prepared composites were evaluated under controlled laboratory conditions. The findings collectively suggested that the epoxy composites reinforced with 20 wt% of AEVFs exhibited promising characteristics for lightweight structural applications.

Design and Validation of a Stratified Shear Model Box for Seismic Response of a Sand-Blowing Reclamation Site

The global increase in building collapses and damage on soft-soil sites due to distant significant earthquakes poses similar challenges for sand-blowing reclamation (SBR) sites on soft-soil layers. This study was initiated to capture the vibration characteristics of the SBR sites and to provide fresh insights into their seismic responses. Initially, considering the heterogeneity and layered structure of soil at SBR sites, we developed a novel stratified shearing model box. This model box enables the simulation of the complex characteristics of soil layers at SBR sites under laboratory conditions, representing a significant innovation in this field. Subsequently, an innovative jack loading system was developed to apply active vertical pressure on the soil layer model, accelerating soil consolidation. Furthermore, a new data collection and analysis system was devised to monitor and record acceleration, pore water pressure, and displacement in real time during the experiments. To verify the model box’s accuracy and innovation, and to examine the seismic response of SBR sites under varying consolidation pressures, four vibration tests were conducted across different pressure gradients to analyze the model’s predominant period evolution due to consolidation pressures. The experimental results demonstrate that the model box accurately simulates the propagation of one-dimensional shear waves in soil layers under various consolidation pressures, with notable repeatability and reliability. Our experiments demonstrated that increasing consolidation pressure results in higher shear wave speeds in both sand and soft-soil layers, and shifts the site’s predominant period towards shorter durations. Concurrently, we established the relationship between the site’s predominant period and the input waves. This study opens new paths for further research into the dynamic response properties of SBR sites under diverse conditions through shaking-table tests.

Toxicity of Diuron, Diquat and Terbutryn to Cyanobacterial Mats

This study investigated acute toxicity of Diuron, Diquat and Terbutryn to cyanobacterial mats collected from Wadi Gaza, Palestine. Toxicity was measured by the reduction of population growth of cyanobacterial mats exposed to various herbicide concentrations in the range of 0-15.44 μmole L-1. Optical density of cyanobacterial suspension was recorded using a spectrophotometer at 680 nm from time zero up to 8 days and taken as an indicator of growth. EC50 and ET50 values were estimated using linear regression equations. Results showed that cyanobacterial mats adapted and grew fast under laboratory conditions. The toxicity of Diuron, Terbutryn and Diquat reached 89.35, 76.02 and 71.24%, respectively. Toxicity of binary mixtures (Diuron and Diquat) decreased from 77.35% to 65.33% due to the reduction of Diuron concentration in the mixture. EC50 values for Diuron, Terbutryn and Diquat were 0.009, 0.031and 0.381 μmole L-1 whereas ET50 values were 32.32, 35.89 and 23.45 h, respectively. EC50 values of binary mixtures (B1-B3) were 0.004, 0.01 and 0.012 toxicity units. Tertiary mixtures (T1-T7) had a wide range of EC50 (0.043-2.9E-07) TUs. The mixture toxicity index (MTI) had positive and negative value in mixtures, indicating synergetic and antagonistic effects. Application of the above-mentioned herbicides may severely damage the ecosystem. Our results are the first of its kind in the region and may be considered as a guideline for future studies.

Vairimorpha (Nosema) ceranae can promote Serratia development in honeybee gut: an underrated threat for bees?

The genus Serratia harbors opportunistic pathogenic species, among which Serratia marcescens is pathogenic for honeybees although little studied. Recently, virulent strains of S. marcescens colonizing the Varroa destructor mite's mouth were found vectored into the honeybee body, leading to septicemia and death. Serratia also occurs as an opportunistic pathogen in the honeybee's gut with a low absolute abundance. The Serratia population seems controlled by the host immune system, but its presence may represent a hidden threat, ready to arise when honeybees are weakened by biotic and abiotic stressors. To shed light on the Serratia pathogen, this research aims at studying Serratia's development dynamics in the honeybee body and its interactions with the co-occurring fungal pathogen Vairimorpha ceranae. Firstly, the degree of pathogenicity and the ability to permeate the gut epithelial barrier of three Serratia strains, isolated from honeybees and belonging to different species (S. marcescens, Serratia liquefaciens, and Serratia nematodiphila), were assessed by artificial inoculation of newborn honeybees with different Serratia doses (104, 106, and 108 cells/mL). The absolute abundance of Serratia in the gut and in the hemocoel was assessed in qPCR with primers targeting the luxS gene. Moreover, the absolute abundance of Serratia was assessed in the gut of honeybees infected with V. ceranae at different development stages and supplied with beneficial microorganisms and fumagillin. Our results showed that all tested Serratia strains could pass through the gut epithelial barrier and proliferate in the hemocoel, with S. marcescens being the most pathogenic. Moreover, under cage conditions, Serratia better proliferates when a V. ceranae infection is co-occurring, with a positive and significant correlation. Finally, fumagillin and some of the tested beneficial microorganisms could control both Serratia and Vairimorpha development. Our findings suggest a correlation between the two pathogens under laboratory conditions, a co-occurring infection that should be taken into consideration by researches when testing antimicrobial compounds active against V. ceranae, and the related honeybees survival rate. Moreover, our findings suggest a positive control of Serratia by the environmental microorganism Apilactobacillus kunkeei in a in vivo model, confirming the potential of this specie as beneficial bacteria for honeybees.

Bemisia tabaci, (Gennadius, 1889) occurrence on soybean cultivars in Brazil

Soybeans are a major grain crop in Brazil, crucial for global food security. However, it faces significant threats from pests like the whitefly, Bemisia tabaci (Gennadius, 1889) (Hemiptera: Aleyrodidae). Currently, whitefly control relies heavily on chemical insecticides, leading to an escalation in sprays and the emergence of resistant populations. This study aimed to assess the incidence of whiteflies on different soybean cultivars throughout the crop cycle. Adult whiteflies were counted weekly on soybean leaves, while nymphs were evaluated on leaflets in laboratory conditions. The results revealed that cultivars DM 5958, BMX ZEUS, M 5947, TMG 7262, and DM 66i68 exhibited the highest incidence of B. tabaci.

Considerations on the Evolutionary Biology and Functions of Eosinophils: What the 'Haeckel'?

The origins and evolution of the eosinophilic leukocyte has received only scattered attention since Paul Ehrlich first named this granulocyte. Studies suggest that myeloperoxidase, expressed by granulocytes, and eosinophil peroxidase diverged some 60-70 million years ago, but invertebrate to vertebrate evolution of the eosinophil lineage is unknown. Vertebrate eosinophils have been characterized extensively in representative species at light microscopic, ultrastructural, genetic, and biochemical levels. Understanding of eosinophil function continues to expand and includes to date regulation of "Local Immunity And/Or Remodeling/Repair" (the so-called LIAR hypothesis), modulation of innate and adaptive immune responses, maintenance of tissue and metabolic homeostasis, and under pathologic conditions, inducers of tissue damage, repair, remodeling, and fibrosis. This contrasts with their classically considered primary roles in host defense against parasites and other pathogens, and involvement in T-helper 2 inflammatory and immune responses. The eosinophils' early appearance during evolution and continued retention within the innate immune system across taxa illustrate their importance during evolutionary biology. However, successful pregnancies in eosinophil-depleted humans/primates treated with biologics, host immune responses to parasites in eosinophil-deficient mice, and the absence of significant developmental or functional abnormalities in eosinophil-deficient mouse strains under laboratory conditions, raise questions of the continuing selective advantages of the eosinophil lineage in mammals and humans. The objectives of this review are to provide an overview on evolutionary origins of eosinophils across the animal kingdom, discuss some of their main functions in the context of potential evolutionary relevance, and highlight the need for further research on eosinophil functions and functional evolution.