Objectives: To determine the effectiveness of utero-tonic drugs use educational program on nurses- midwives' knowledge and to predict the variables which may affect their knowledge. Method: A quasi experimental study was conducted from 1st May 2021to 10th March 2022 in two hospitals at al- kut city on nurses and midwives who work at maternity hospitals. Non- probability (Convenience sampling) was used to collect the data from select 65 nurses and midwives, (32) of them for study group and (33) for control group. A questionnaire is an instrument to collect the demographic, occupational information and knowledge which consists of 40 items. An educational program is designed to involve two components which are: General information about utero-tonic drugs and Common use drugs (indication, side effects, contraindications, complications from misuse, and nursing interventions) and it was implemented on the study group only. Pretest and post-test had been conducted before and after the implementation of program for both groups. Validity of content was determined through reviewing it by (13) experts and reliability of tools was determined through a pilot study descriptive and inferential statistics were used to analyze the data.