In this study, high intensity ultrasound (HIU), antibrowning chemicals (calcium chloride and ascorbic acid), water bath were used to avoid the browning of kiwifruit pieces, and the shelf-life of fresh-cut was measured (two weeks). The treated fresh-cut kiwi samples were evaluated in terms of color, enzyme activities, bioactive compounds, microbial and sensory studies. The study shown that HIU treatment restricted (p<0.05) the microbial growth (bacteria, yeats & mold) in fresh-cut kiwi samples at 4 °C. Moreover, kiwi samples exposed to HIU exhibited significant (p<0.05) enhancements in bioactive metabolites as well as improvements in the physical features with the comparison of all other applications. Similarly, the lowest browning index (BI) values were observed for kiwifruit samples by HIU at the end of storage (180.04). Furthermore, the lowest scores for decay (0.5/3.0 and 0.9/3.0 in days 7 and 14, respectively) and off-odor (1/7 and 2/7 in days 7 and 14, respectively) were determined for the fresh-cut kiwifruit subjected to HIU. It has been proposed that HIU application is a possible choice to take the place of chemical and physical approaches to extend the product life and maintain quality characteristics in fresh-cut kiwifruit at cold storage conditions.