Abstract The aim of this study was to investigate the effect of hempseed cake on production traits, metabolic profile and antioxidant status of lambs. The research was conducted on 20 Merinolandschaf lambs of similar live body weight (24.03 kg ± 2.22 and 24.06 ± 1.84 kg in control and experimental group, respectively). The experiment started after the weaning of lambs at their average age of 70 days. Lambs were divided by gender (50% male: 50% female) and then allocated in two groups. The research lasted for 30 days during the fattening period. Lambs in the control group were fed with feed mixtures, where main protein source was soybean meal and extruded soybean (SB), while lambs in the experimental group were fed feed mixtures in which soybean was replaced by hempseed cake (HSC) at 12% of dry matter. Higher values of daily weight gain (P<0.05) compared to SB lambs were observed from days 15 to 30. HSC lambs showed no significant differences in hematology and biochemical blood parameters, except for lower (P<0.001) urea concentrations on days 15 and 30, as well as higher (P<0.05) Mg concentrations in serum on day 30. Hempseed cake in lambs’ diets did not influence (P>0.05) activity of the enzymes like AST, ALT, ALP, GGT and CK, as well as SOD and GPx enzymes, thus not affecting the antioxidant status of lambs’ blood. Concentrations of DHA and n-3 fatty acids were higher (P<0.05) in HSC group than in SB group. The present study confirmed that the inclusion of HSC as soybean replacement up to 12% in the lambs’ diet is justified when considering production traits, metabolic profile and antioxidant status of Merinolandschaf lambs.