Ethnobotany is an interaction relationship between ethnic and plants. The Dayak Kayaan and Dayak Bukat communities in Datah Dian Village, North Putussibau District, Kapuas Hulu Regency have different knowledge in utilizing and managing plants for traditional ritual activities. Research purpose was to obtain the ethnobotanical data utilized by the Dayak Kayaan and Dayak Bukat communities for traditional rituals in Datah Dian Village, North Putussibau District, Kapuas Hulu Regency. The method used in this research was a survey method with snowball sampling technique by interviews with the community and assisted by questionnaire. The results showed that the plants used by the Dayak Kayaan Tribe as many as 26 species and Dayak Bukat Tribe were obtained 9 species of plants. Plant utilization performed by Dayak Kayaan community there are 4 activities namely for gawai, birth, death, and marriage. The most widely used species of plants was tree habitus with 13 species and parts of plants that are used mostly use were stem, leaves and all parts of plant with percentage of each 27%. The utilization of plants performed by the Dayak Bukat community was 5 activities that were for gawai, birth, death, marriage, and to open the fields. The most widely used species of plants was the herb habitus form as many as 5 species and parts of plants that were most widely utilized was leaf with a percentage of 56%. The implementation of traditional rituals by Dayak Kayaan and Dayak Bukat communities is different, caused by different Dayak families.Keywords : Dayak Kayaan, Dayak Bukat, Ethnobotany, Traditional Ritual