In-vitro Research Articles

Investigation of an outbreak of Elizabethkingia meningoseptica on a pediatric intensive care unit.

Objective: This paper reports an Elizabethkingia meningoseptica outbreak on a pediatric intensive care unit with emphasis on investigation of outbreak source, infection control interventions, patient characteristics and comparative antimicrobial susceptibility results.Methods: This was an ambidirectional cohort study conducted in a university hospital 20-bed pediatric intensive care unit. Patient ages ranged from 4 to 11 months, with a median age of 9 months. 83% of the patients had severe underlying conditions. Samples from staff and environmental surfaces were obtained to identify a common source of infection. Antimicrobial susceptibility tests of isolated bacteria were done using the disk diffusion method and the Vitek®2 automated system.Results: Environmental surveillance revealed contamination of the water reservoirs of two different mechanical ventilators. In-vitro antimicrobial susceptibility testing results with two different methods (Vitek®2 and disk diffusion) were coherent for most of the investigated antibiotics, but without coherence for ciprofloxacin and levofloxacin. Resistance was found to the relatively new antibiotics ceftaroline and ceftazidime-avibactam.Conclusions: E. meningoseptica is a significant cause of nosocomial infections, with high mortality especially in children. Investigation of the outbreak source and continuation of intensive infection control precautions are vital to handle E. meningoseptica outbreaks in PICUs. Using quinolones according to testing results of automated AST systems may lead to inadequate treatment and foster the selection of resistant strains.

SP-019 - Synthesis and preliminary in-vitro studies of [99mTc][TcN(PNP)]-biphosphonates as new bone-radiopharmaceuticals.

SP-019 - Synthesis and preliminary in-vitro studies of [99mTc][TcN(PNP)]-biphosphonates as new bone-radiopharmaceuticals.

New approach for T-shaped uterus: Metroplasty with resection of lateral fibromuscular tissue using a 15 Fr miniresectoscope. A step-by-step technique.

T-shaped uterus is a congenital uterine malformation (CUM), only recently defined by the ESGE ESHRE classification as Class U1a. The uterus is characterised by a narrow uterine cavity due to thickened lateral walls with a correlation 2/3 uterine corpus and 1/3 cervix. Although the significance of this dysmorphic malformation on reproductive performance has been questioned, recent studies reported significant improvement of life birth rates after surgical correction in patients with failed in-vitro fertilisation (IVF) or recurrent miscarriage. The classical surgical technique to treat a T-shaped uterus is by performing a sidewall incision with the micro scissor or bipolar needle, resulting in a triangular cavity.In this video article, we describe a new surgical technique with a step-by-step method combining three- dimensional ultrasound (3D-US) and hysteroscopic metroplasty in an office setting, using a 15 Fr office resectoscope (Karl Storz, Tuttlingen, Germany), to treat a T-shaped uterus by resecting the lateral fibromuscular tissue of the uterine walls. No complications occurred and the postoperative hysteroscopy showed a triangular and symmetrical uterine cavity without any adhesions.

Сохранение видов растений Красной книги России ex situ в ботанических садах

Russian botanical gardens are actively involved in the conservation of rare and endangered plants. They pay special attention to the species included in the Red Book of the Russian Federation. At present, 377 species of higher plants are cultivated in living collections of botanical gardens out of 514 species presented in the Red Book of Russia, which is 73%. Thus, the Russian Federation has practically met the requirement of goal 8 of the Global Strategy for Plant Conservation. The vast majority of rare plant species are represented by samples in the collections of many botanical gardens and the reliability of their protection under cultural conditions is beyond doubt. In the Main Botanical Garden of the Russian Academy of Sciences, an in vitro collection of rare and endangered plants is preserved, including 82 species, which is 17.3% of the total number of angiosperms included in the Red Book of the Russian Federation. As a result of many years of research, the features of the cultivation and preservation of ex situ plants belonging to different families have been revealed. The main methodological aspects at the stages of obtaining a sterile culture, micropropagation proper and long-term deposition are reflected. The compositions of nutrient media and cultivation factors were optimized for slowed growth of explants of the studied cultures and preservation of their viability. Keywords: RARE AND ENDANGERED PLANT SPECIES, RED BOOK OF THE RUSSIAN FEDERATION, EX SITU CONSERVATION, LONG-TERM IN VITRO CONSERVATION

Degree of Conversion of Two Universal Adhesives Incorporated with Ascorbic Acid Coated Superparamagnetic Nanoparticles Assessed by FTIRDegree of conversion, universal adhesives, Superparamagnetic Fe3O4nanoparticles, FTIR analysis

Aims: To prepare and characterize a nano-ferro fluid of ascorbic acid coated superparamagnetic Fe3O4nanoparticles and incorporate them into two universal dental adhesives to test their effect on the immediate and 24-hours post-cure Degree of Conversion (DC) in comparison to their control non-incorporated adhesives. Study design: An invitro study.Methods and Material: A colloidal nano ferro fluid of ascorbic acid coated superparamagnetic nanoparticles was prepared and characterized by transmission electron microscopy and incorporated into two universal adhesives. Thirty-two adhesive samples in PotassiumBromide (KBr) discs were prepared for FTIR analysis, and distributed as following, i.e., GI: Eight adhesive samples of non-incorporated All Bond Universal (Control), GII: Eight adhesive samples of 2%-incorporated All Bond Universal, GIII: Eight adhesive samples of non-incorporated Prime&Bond Universal (Control), GIV: Eight adhesive samples of 2%-incorporated Prime&Bond Universal.Equal amount (25μL) of each adhesive was placed on KBr disc, the solvents was evaporated by a gentle steam of air, and then a second KBr disc was mounted over the first one and pressed softly to form a thin adhesive film. Finally, the KBr discs containing the adhesive were placed in the demountable cell holder and mounted into the sample chamber of the FTIR spectrometer. FTIR spectra was then recorded for each adhesive before, immediately after, and 24-hours post-curing to calculate the degree of conversion.Statistical analysis used: Statistical analysis was conducted using independent variable t-test by the IBM-SPSS software version 22.Results: The study results showed good DC values for the incorporated adhesives with no statistically significant differences in the degree of conversion values (i.e. Immediate and 24-hours post-cure) in comparison to control groups for both adhesives. The Prmie&Bond universal (Both control and incorporated) revealed significantly higher DC than All bond universal.Conclusions: Incorporation of ascorbic acid coated Fe3O4nanoparticles at 2% by mass into the universal adhesives resulted in good degreeof polymerization with no significant difference in comparison to their commercial controls.

EVALUATION OF IMPACT STRENGTH OF HEAT CURE ACRYLIC RESIN REINFORCED WITH NYLON FIBER MESH WITH AND WITHOUT PRESTRESSING (IN VITRO STUDY)

INTRODUCTION: Acrylic resin prostheses are liable to high levels of forces due to their frequent removal by patients and accidental slippage. Hence, the impact strength of acrylic denture base material is of major significance in providing long-term functional prostheses.OBJECTIVES: The aim of this study was to evaluate the reinforcing effect of prestressed nylon fiber meshwork and the specimen thickness increase on the impact strength of heat cure acrylic resin.MATERIALS AND METHODS: 72 specimens were divided into 3 groups (I, II and III) which were heat cure acrylic resin (control), Nylon mesh reinforced acrylic resin and prestressed-nylon mesh reinforced acrylic resin. Each group was formed of 24 specimens, which were further subdivided into 3 subgroups (A, B and C) of different dimensions; 75 mm × 10 mm × 3 mm and 75 × 10 × 4 mm and 75 x 10 x 5 mm. Thus, the specimens were divided into 9 experimental subgroups (n=8 per subgroup). A Charpy’s impact tester was used to measure the impact strength of acrylic resin. The data were analyzed using Anova-test (p≤0.05).RESULTS: The mean impact strength of groups II and III was higher than the control group I impact strength. The difference between the reinforced groups and control group was statistically significant for all specimen thicknesses.CONCLUSIONS: The impact strength of heat cure acrylic resin was increased by the incorporation of nylon fiber mesh whether prestressed or not.

Two Faces of Milk Proteins Peptides with Both Allergenic and Multidimensional Health Beneficial Impact- Integrated In Vitro/In Silico Approach.

The main food-origin antigens that the infant’s body is in contact with are cow’s milk proteins (CMP). Still, CMP are one of the main sources of beneficial biologically active peptides that play a role in treatment of non-communicable diseases. Safe methods to quickly predict the sensitizing potential of food proteins among their range of health-promoting properties are essential. The aim of study was to adapt an integrated approach combining several in silico (IS) studies and in vitro (IV) assays to screen the multifunctionality of CMP-derived peptides. Major histocompatability complex type II MHC II-binders, interleukin-4 and -10 inducers, interferon γ -inducers and immunobioactivity tools were used to predict the peptide-power of inducing allergies or tolerance. A comparison of the peptide profiless revealed the presence of one identical and one overlapping sequence in IS and IV hydrolysate. By IS analysis, four of 24 peptides were found to have high affinity and stimulate IL-4 expression, and by IV, one of seven peptides had this potential (Bos d9 peptide DIPNPIGSENSEK (195–208)). Three IV peptides may induce IL-10 expression. The IV/IS assessment seems promising agents for peptides’ potential determination dedicated only to preliminary screening of peptides. The IV verification is still crucial in further steps of studies.

IN VITRO СКРИНИНГ ЗА ХЕРБИЦИДНА СЕЛЕКТИВНОСТ НА НОВИ МУТАНТНИ ГЕНОТИПОВЕ ПИПЕР С РАЗЛИЧЕН ПРОИЗХОД И ОЦВЕТЯВАНЕ НА ПЛОДА

The study presents an in vitro test development as a model for herbicide phytotoxicity. It provides reliable data on how the herbicide affects the seed germination and early growth stages, in dynamic, during the cultivation. The sensitivity of five mutant pepper genotypes with different origin and fruit colour to the herbicide napromamide (Devrinol 4F) was investigated. All studied herbicide doses, corresponding to 3, 4, and 5 L/ha, caused phytotoxicity expressed by decreased germination and growth inhibition. A strong genotype dependence was established – two breeding lines (18 and 85), originating from local populations, demonstrated a low sensitivity to the herbicide, additionally confirmed by higher plant survival and adaptability after transplanting to soil ex vitro.

Technical note: Effects of filter bags on neutral detergent fiber recovery and fiber digestion in vitro

Filter bags facilitate the measurement of amylase-treated neutral detergent fiber (aNDF) and in vitro (IV) undigested aNDF (uNDF) by eliminating the transfer of residues from beakers into filtration crucibles. The objectives of this study were to (1) determine effects of filter bags on recovery of aNDF and (2) evaluate effects of filter bags on IV uNDF. For study 1, 6 samples each of grass hay (GR), alfalfa (AL), and corn silage (CS) were selected. Large standard deviations (SD) of ash-free aNDF (aNDFom) for samples in each forage type indicated compositional diversity (15.1, 7.45, and 12.9% of DM for GR, AL, and CS, respectively), and starch SD for CS was 16.4% of DM. Samples were weighed into Berzelius beakers or filter bags [25-µm pores (F57) or 6-µm pores (F58); Ankom Technology, Macedon, NY] for measurement of aNDF and aNDFom. All samples were extracted with neutral detergent, thermostable α-amylase, and sodium sulfite, and then soaked in boiling water and then acetone. Residues from beakers were filtered through a sea sand-covered GF/D filter (Whatman, Marlborough, MA) in Gooch crucibles (CR). Filter bags were extracted in a pressurized chamber at 100°C. The aNDF values did not differ between F57 and CR, but F58 was greater than CR for CS and AL. For GR, F58 was greater than CR for aNDFom. For study 2, diverse samples with large SD of aNDFom (20.7, 7.45, and 12.9% of DM for GR, AL, and CS, respectively) were weighed as loose powder into medium bottles (LS) or F57 bags, which were weighted to prevent floating. Blended ruminal fluid from 3 steers fed a 30% aNDFom diet was used as inoculum. Three samples of 1 forage type were randomly assigned to 1 of 6 IV runs using both treatments (LS and F57), and 3 bottles of each sample-treatment combination were removed after 12 h and 2 were removed after 120 h to measure uNDF. For LS, residues were extracted as in study 1 for CR. For F57, bags were rinsed in cold water and extracted as described in study 1. After 12 h, uNDF of F57 was greater than LS in CS, AL, and overall types. Ash-free uNDF (uNDFom) after 12 h of F57 was greater than LS in CS and overall types. After 120 h, F57 was greater than LS for uNDF of CS, but no differences were detected for uNDFom. The SD of uNDFom, but not uNDF, was higher after 12 and 120 h for F57 compared with LS. From 6 to 96 h, overall gas production of F57 was less than LS, and F57 was less than LS for CS from 3 to 96 h. Overall, LS gave greater maximum and faster rates of gas production than F57, as did AL and CS, but lag did not vary. Results indicate that filter bags affected aNDF and aNDFom measurement and inhibited fermentation for some materials.

Microstructural and histochemical variations during in vitro to in vivo plant developments in Aloe vera (L.) Burm.f (Xanthorrhoeaceae)

Aloe vera (L.) Burm.f (Barbados aloe) is an economically important succulent herb, well accredited for its medicinal properties for centuries. In vitro propagation encouraged the production of identical individuals within a short period. However, there are evidences of in vitro induced disorders in the morphology, anatomy, phytochemistry and physiology of the micropropagated plantlets. These anomalies severely affect the acclimatization process and lead to loss of tissue culture raised plants in the field. This study, therefore, sought to evaluate the foliar micro-morpho-anatomical and histochemical changes during various phases of in vitro plant regeneration of A. vera. These variations were analyzed at three different stages of growth and development of the plantlets from lab to land [in vitro (IV), ex vitro (EV), and field transferred (FT) plants]. The field transferred plantlets showed improved phenotype, foliar micro-structures, leaf pigmentation (chlorophylls and carotenoids), and histochemical depositions in terms of polysaccharides (starch and mucilage), phenolic compounds (tannins, polyphenol, and lignin), and cutin and suberin as compared with in vitro and ex vitro plantlets. The study would allow us to understand the appropriate stage of secondary metabolites accumulation in the tissues and the successful establishment of plantlets in soil. The present findings outline the long-term effort to assess the potential of mass propagation (in vitro) of A. vera for commercial cultivation practices.

Advances of Polar and Nonpolar Extracts of Salix (Salix mucronata) and Their In-Vitro Biological Applications

Advances of Polar and Nonpolar Extracts of Salix (Salix mucronata) and Their In-Vitro Biological Applications

Design, Development and In-vitro Characterization of Floating tablets of Propranolol hydrochloride

Design, Development and In-vitro Characterization of Floating tablets of Propranolol hydrochloride

MELİTTİN VE KANSER TEDAVİSİ: NANOTEKNOLOJİK BAKIŞ AÇISI

Kanser insanları olumsuz etkileyen Dünya’da önemli bir morbidite ve mortalite kaynağıdır. Bu hastalıkla mücadele edebilmek amacıyla pek çok farklı doğal kaynaklı üründen faydalanılmaktadır. Son yıllarda gelişen teknolojiyle beraber bu doğal kaynaklı ürünlerden elde edilen bileşiklerin anti-kanser etkilerinin moleküler mekanizmalarının aydınlatılması hedeflenmiştir. Bu doğal ürünlerden bir tanesi olan ve sitolitik etki gösteren arı zehri bileşenlerinden faydalanılmaktadır. Arı zehri 15’ten fazla farklı peptit ve protein içermekle beraber bunlarda melittin ve fosfolipaz-A2 sitolitik özelliklerinden ötürü çok daha ilgi çekmektedir. Bu bileşenlerin spesifikliğinin artırılması amacıyla çeşitli nanoteknolojik platformlar sunulmuştur. Bu derlemede henüz çok güncel ve halen geliştirilmekte olan arı zehri bileşenlerinin çeşitli kanser türleri üzerinde in-vitro ve in-vivo uygulamalarına dair mevcut literatür bilgisi nanoteknolojik gelişmeler açısından bir araya getirilmiştir.

Assessing the impacts on fetal dosimetry of the modelling of the placental transfers of xenobiotics in a pregnancy physiologically based pharmacokinetic model

The developmental origin of health and diseases theory supports the critical role of the fetal exposure to children's health. We developed a physiologically based pharmacokinetic model for human pregnancy (pPBPK) to simulate the maternal and fetal dosimetry throughout pregnancy. Four models of the placental exchanges of chemicals were assessed on ten chemicals for which maternal and fetal data were available. These models were calibrated using non-animal methods: in vitro (InV) or ex vivo (ExV) data, a semi-empirical relationship (SE), or the limitation by the placental perfusion (PL). They did not impact the maternal pharmacokinetics but provided different profiles in the fetus. The PL and InV models performed well even if the PL model overpredicted the fetal exposure for some substances. The SE and ExV models showed the lowest global performance and the SE model a tendency to underprediction. The comparison of the profiles showed that the PL model predicted an increase in the fetal exposure with the pregnancy age, whereas the ExV model predicted a decrease. For the SE and InV models, a small decrease was predicted during the second trimester. All models but the ExV one, presented the highest fetal exposure at the end of the third trimester. Global sensitivity analyses highlighted the predominant influence of the placental transfers on the fetal exposure, as well as the metabolic clearance and the fraction unbound. Finally, the four transfer models could be considered depending on the framework of the use of the pPBPK model and the availability of data or resources to inform their parametrization.

Accuracy in clinical examinations for the diagnosis of vulvovaginitis by CANDIDA SPP. and IN VITRO susceptibility to the main antifungals

Vulvovaginal candidiasis (VVC) is a common infection. This work aims to determine the positive predictive value (PPV) of the clinical diagnosis of VVC and to characterize Candida species isolated from the vaginal mucosa. This cross-sectional study was conducted from February 2016 to February 2017 at the Gynecology and Obstetrics Outpatient Clinic of the Hospital das Clínicas, in Goiânia, Goiás State, Brazil. The study included samples of vaginal secretion from 55 women who complained of vaginal discharge and itching as their main symptoms. The PPV of the clinical diagnosis of VVC was estimated in comparison to the laboratory culture method. The phenotypic methods and molecular tests were performed to identify Candida spp. In vitro susceptibility of Candida spp. isolates to fluconazole, itraconazole, clotrimazole, nystatin, and amphotericin B was determined using the broth microdilution assay. Yeast growth using the enzymes protease, phospholipase, and hemolysin was carried out in media containing respectively bovine albumin, egg yolk, and sheep erythrocytes. A PPV of 61.8% (34/55) was determined. Among the 55 vulvovaginal samples collected, we identified 36 isolates in whichC. albicans was the most common species. High resistance to fluconazole and low minimal inhibitory concentration (MIC) values for clotrimazole, nystatin and amphotericin B were observed. All isolates were proteinase and hemolysin producers, while seven strains were phospholipase negative. The clinical diagnosis of VVC presented a moderate PPV, which meant that cultures had to be conducted in the laboratory to confirm infection. The high resistance to fluconazole and itraconazole indicated the importance of the in vitro susceptibility test.KEY WORDS: Vulvovaginal candidiasis; antifungal susceptibility; enzymatic activity

Allelopathy Effect of Trichoderma spp. and Some Plant Extracts against Pythium aphanidermatum (In-vitro)

Background: Serious seed-borne and soil-borne pathogens are existing at the rhizosphere area, many species of Pythium can cause seed rot and damping-off disease. The application of plant extracts and biological agents as alternative ways for the management of plant diseases has recently become an important variable component for Integrated Pest Management, so plant metabolites regarded as eco-friendly where plants play an important role in the control of diseases. The current study amid to evaluate the allelopathy effect of two species of biological agents particularly Trichoderma spp. and some plant extracts against the soil-borne pathogen P. aphanidermatum on cucumber seedlings under laboratory conditions. Methods: In this study laboratory investigation during 2018, used the allelopathy effect of two species of Trichoderma (T. harzianum and T. hamatum) and five plant extracts, in Three conc. 10.000, 20.000 and 30.000 ppm, to control damping-off disease in cucumber caused by Pythium aphanidermatum instead of using chemicals fungicides. Result: Our study observed that Trichoderma spp., have a high inhibitory effect on the tested pathogen, but in the assay of plant extracts, only some with different concentrations had a significant effect against P. aphanidermatum (in-vitro). Cinnamomum cassia, Syzygium aromaticum and Eucalyptus globulus were most affective from all other five tested plant extracts, where the concentration of 30.000 ppm gave the highest inhibitory effect against P. aphanidermatum, while Psidium guajava and Lawsonia inermis extracts showed a lower inhibitory effect on radial growth of P. aphanidermatum.

Effect of Thickness and Shade of Resin and Ceramic- Based Hybrid Materials on Color Masking Abilities and Optical Performance of CAD/CAM Materials.

Scientific data analysing color masking abilities of chairside CAD/CAM materials is lacking. The purpose of this in-vitro study was to evaluate the thickness and shade influence of three materials on their optical behaviour. Three materials: a) LD: Lithium disilicate glass ceramic (Emax, Ivoclar Vivadent), b) LDS: Lithium-disilicate-strengthened aluminosilicate glass ceramic (N!ce, Straumann) and c) RNC: Resin Nanoceramic (Lava Ultimate, 3M ESPE) were polished in different shades (A1,A2,A3) and thicknesses (0.1- 1.2mm). Specimens (N=108; n=36 per group) (12x12x1mm3) were positioned on resin composite base (Clearfil AP-X, Kuraray) in shade A3. Spectrophotometric measurements were performed and the parameters thickness, shade and material were analysed using three-way ANOVA, and pairwise T-tests (P-values ⟨ 0.05). Both the shade (p⟨0.001) and the interaction of material in correlation to thickness (p⟨0.001) were significant. Shade value A1-A3 comparisons were significant A1 vs. A2 (p=0.045); A2 vs. A3 (p=0.002); A1 vs. A3 (p⟨0.001)). A significant correlation of the material and thickness was observed when comparing LD and LDS (p=0.007) at the thickness of 0.1-0.4 mm. Masking abilities were influenced by material and thickness choice. Reinforced glass ceramics showed the best results in the smallest thickness tested (0.1-0.4 mm). LDS could be considered as an advantageous alternative in minimal-invasive cases.

Ceftriaxone-sulbactam-EDTA susceptibility profile of multi-drug resistant gram-negative bacterial isolates: Experience from a tertiary care teaching hospital in Rishikesh, Uttarakhand

Introduction: Studies have shown that ceftriaxone-sulbactam-EDTA combination is a promising therapeutic option as carbapenem sparer in cases of infections caused by ESBL and MBL producing pathogens, respectively. This study is aimed to generate preliminary data on in-vitro susceptibility profile of clinical multi-drug resistant (MDR) Gram-negative bacterial isolates to ceftriaxone-sulbactam-EDTA combination. Materials and Methods: A cross-sectional study was conducted from January 1st, 2019 to October 31st, 2019. Antibiotic susceptibility data (including that of ceftriaxone-sulbactam-EDTA combination) of 200 multi-drug-resistant Gram-negative bacterial isolates obtained from various nonrepetitive clinical samples of patients of all age groups and sexes, was retrospectively analyzed. All clinical samples were processed aerobically as per standard guidelines, and the bacterial isolates obtained in culture were identified by conventional biochemical methods. Antibiotic susceptibility testing of bacterial isolates was performed using the modified Kirby Bauer disk diffusion method, the results of which were interpreted as per the Clinical and Laboratory Standards Institute (CLSI) guidelines 2019. In vitro susceptibility test results of ceftriaxone-sulbactam-EDTA combination, disks were interpreted as per the manufacturer's instructions. Results: Acinetobacter spp. was the most common isolate (53%), followed by Escherichia coli (20.5%), Klebsiella spp. (17.5%), Pseudomonas aeruginosa (16.5%), Citrobacter spp. (2%), and Proteus spp. (1%), respectively. 99.1%, 92.7%, 88.6%, and 69.3% of Acinetobacter spp., E. coli, Klebsiella spp. and P. aeruginosa, respectively, were susceptible to ceftriaxone-sulbactam-EDTA combination disks. Conclusions: The preliminary data generated by our study could be an eye-opener for clinicians practicing in this part of the country and should prompt further investigation in the form of clinical trials.

IN VITRO EVALUATION OF ELECTROSPUN POLYSACCHARIDE BASED NANOFIBROUS MATS AS SURGICAL ADHESION BARRIERS

Postoperative adhesions are one of the most important problems that the patients and surgeons face. In this study, nanofibrous mats as a novel surgical adhesion barrier were produced from polysaccharide-based polymers, hyaluronic acid, carboxymethyl cellulose and sodium alginate, via electrospinning. The produced nanofibrous mats were crosslinked with 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride and N-hydroxysulfosuccinimide. Furthermore, the morphology, and the in vitro degradation, cytotoxicity and cell adherence potential of the nanofibrous mats aimed to be used as adhesion barriers were evaluated and compared with a commercial adhesion barrier. After the crosslinking process, the nanofibrous structure was maintained, and the diameters of resulting nanofibers were below 300 nm. Results of the in vitro experiment showed that the nanofibrous mats have maintained their physical structures during the critical period for adhesion formation, and had non-adherent cell feature and non-cytotoxic nature required for an ideal adhesion barrier.

STUDY OF BIOFILM RESPONSE OF OLEA EUROPAEA TO UV RADIATIONS: AN IN VITRO APPROACH

STUDY OF BIOFILM RESPONSE OF OLEA EUROPAEA TO UV RADIATIONS: AN IN VITRO APPROACH