Temperature-gradient-mechanical fatigue (TGMF) is a significant concern in internal cooling turbine blades. In the present study, the through-thickness temperature gradient was determined by the inverse heat conduction method and investigated in TGMF. Using the continuum damage mechanics, the environmental impact was identified as the primary contributor to the in-phase TGMF damage. A damage accumulation model encompassing fatigue, creep and environmental impact was established and provided satisfactory accuracy in TGMF life assessments. The main conclusion is that temperature gradients do not change the fracture mode but significantly increase stress levels by at least 20%, accelerating oxidation damage and resulting in a TGMF life ca. 10 times shorter than that of TMF.