Two sources of interference in children's discrimination learning were investigated: reinforcer cue properties, such as size, shape, and texture, and reinforcer incentive properties, linked to preference value. The effect of the former was studied by comparing a condition in which S received the same type of reinforcer for all correct responses with two multiple reward conditions, like those used by Shepp (1962), in which the reward varied from trial to trial between reinforcers differing in their cue properties. Incentive properties were investigated under multiple reward conditions by comparing reinforcers of equal preference value with those of unequal value. Results indicated that variation in both the cue and the incentive properties of rewards influences discrimination performance. The conditions in which reinforcer cue properties were varied, the multiple reward groups, produced poorer performance than the single reinforcer condition. In addition, where the occurrence of multiple rewards was correlated with irrelevant position cues, rewards of unequal, but not equal, preference value induced an interfering response strategy related to reinforcer-position contingencies.