Pruritus after burn injury is commonly reported by pediatric burn survivors. Companion scales of proxy and self-reported itch interference for pediatric patients were recently developed using item response theory (IRT). The purpose of this study was to evaluate the reliability and validity of these measures of itch interference. The brief scales include 4 questions and were developed by burn clinicians and researchers to assess the degree to which itch interferes with children’s activities and quality of life. Data were collected from children with moderate to severe burn injury and their parents/caregivers who participated in a multi-center national longitudinal study. Proxy and self-report items were analyzed separately, and reliability was evaluated by examining the IRT based scale information function. Known group validity analyses were completed by comparing scores by total body surface area burned (TBSA) (<40% vs ≥40%) and pain on a 0–10 item of average pain intensity (0, 1–3, 4–6, and 7–10) using analysis of variance. Data from 192 children with burn injuries and 200 caregivers (91% parents; 76% female) were used. The mean age of children was 8 years (SD:5) and mean TBSA was 45% (SD:18). The pediatric sample was 54% white, 81% Hispanic, and the majority of burns were flame related (76%). Over half of the pediatric sample (56%) reported no problems with itching, while 33% reported itch interference >0.5 SD above the mean and 11% >1.0 SD above the mean. The proxy and pediatric scores were moderately correlated (r=0.63). The scales had excellent reliability (>0.9) between T-scores of 50 and 70 (self) and 49 and 80 (proxy). The scores differentiated between 4 different levels of pain intensity (self and proxy p<0.001), but not between different levels of TBSA. The self-reported and proxy pediatric itch interference scales have high reliability for children or youth with average or higher levels of itch interference, and both are suitable for use in research and clinical practice. The scales are free, brief and clinically relevant measures of itch interference in children and youth with burn injury. The scales can be administered on computer or paper. Children with itch levels >0.5 SD above the mean (33% of participants) likely warrant further evaluation for therapeutic intervention.