Insecticide Treatments Research Articles

Fine Characterization of a Resistance Phenotype by Analyzing TuYV-Myzus persicae-Rapeseed Interactions.

Turnip yellows virus (TuYV), transmitted by Myzus persicae, can be controlled in rapeseed fields by insecticide treatments. However, the recent ban of the neonicotinoids together with the description of pyrethrinoid-resistant aphids has weakened insecticide-based control methods available to farmers. Since the deployment of insecticides in the 1980s, few research efforts were made to breed for rapeseed cultivars resistant to aphid-borne viral diseases. Thus, only few rapeseed cultivars released in Europe were reported to be TuYV-resistant, and the resistance phenotype of these cultivars was poorly characterized. In this study, several epidemiological parameters (infection rate, latency period, etc.) associated to the TuYV-resistance of the cv. Architect were estimated. Results showed a partial resistance phenotype for plants inoculated at the 2-/4-leaves stages and a resistance phenotype for plants inoculated at a more advanced growing stage. Moreover, analysis of infected plants highlighted (i) a poor quality of infected cv. Architect as a source of virus for transmission and (ii) an extended latency period for infected plants. Thus, dynamics of virus spread in the field should to be slower for Architect compared to susceptible rapeseed cultivars, which should lead to the maintenance of a higher proportion of healthy plants in the field.

Functional annotation of Ectropis obliqua transcriptome in the treatment of pyrethroid insecticides

Tea plant is an economic plant, its cultivation has been infected by many agricultural pests, one of which is Ectropis obliqua. A large number of Ectropis obliqua has caused serious harm to many tea areas in China. To elucidate the immune defense and detoxification mechanism of E. obliqua against pyrethroid insecticides, RNA-sequencing was used to analyze transcriptomic data of E. obliqua at different time periods after deltamethrin treatment. Using Illumina sequencing platform, approximately 51,688 clean readings (including 78.23 Gb of sequence data) were obtained from E. obliqua at different time after deltamethrin treatment. These fragments were assembled into 30,601 Unigene with an average length of 1030.43 bp, of which N50 of 1689 bp. Compared with the control group, 1535 differentially expressed genes were found, including 1427 genes up-regulated and 108 genes down regulated. Gene ontology analysis indicated that differentially expressed genes accounted for a high proportion in cell process, single organism process, metabolic process etc. KEGG annotation indicated that differential genes were mainly enriched in basic metabolism needed for life activities and glutathione metabolism and other pathways. The verification by qRT-PCR indicated that the transcriptome data is reliable and can be used for follow-up analysis. Interestingly, it was found that EoGST968 could strongly respond to insecticide stimulation, and the recombinant EoGST968 expressed in prokaryotic cells could catalyze the binding of reduced glutathione (GSH) to1-chloro-2,4-dinitrobenzene (CDNB).

Community deployment of a synthetic pheromone of the sand fly Lutzomyia longipalpis co-located with insecticide reduces vector abundance in treated and neighbouring untreated houses: Implications for control of Leishmania infantum.

The rising incidence of visceral leishmaniasis due to Leishmania infantum requires novel methods to control transmission by the sand fly vector. Indoor residual spraying of insecticide (IRS) against these largely exophilic / exophagic vectors may not be the most effective method. A synthetic copy of the male sex-aggregation pheromone of the key vector species Lutzomyia longipalpis in the Americas, was co-located with residual pyrethroid insecticide, and tested for its effects on vector abundance, hence potential transmission, in a Brazilian community study. Houses within eight defined semi-urban blocks in an endemic municipality in Brazil were randomised to synthetic pheromone + insecticide or to placebo treatments. A similar number of houses located >100m from each block were placebo treated and considered as "True Controls" (thus, analysed as three trial arms). Insecticide was sprayed on a 2.6m2 surface area of the property boundary or outbuilding wall, co-located within one metre of 50mg synthetic pheromone in controlled-release dispensers. Vector numbers captured in nearby CDC light traps were recorded at monthly intervals over 3 months post intervention. Recruited sentinel houses under True Control and pheromone + insecticide treatments were similarly monitored at 7-9 day intervals. The intervention effects were estimated by mixed effects negative binomial models compared to the True Control group. Dose-response field assays using 50mg of the synthetic pheromone captured a mean 4.8 (95% C.L.: 3.91, 5.80) to 6.3 (95% C.L.: 3.24, 12.11) times more vectors (female Lu. longipalpis) than using 10mg of synthetic pheromone. The intervention reduced household female vector abundance by 59% (C.L.: 48.7, 66.7%) (IRR = 0.41) estimated by the cross-sectional community study, and by 70% (C.L.: 56.7%, 78.8%) estimated by the longitudinal sentinel study. Similar reductions in male Lu. longipalpis were observed. Beneficial spill-over intervention effects were also observed at nearby untreated households with a mean reduction of 24% (95% C.L.: 0.050%, 39.8%) in female vectors. The spill-over effect in untreated houses was 44% (95% C.L.: 29.7%, 56.1%) as effective as the intervention in pheromone-treated houses. Ownership of chickens increased the intervention effects in both treated and untreated houses, attributed to the suspected synergistic attraction of the synthetic pheromone and chicken kairomones. The variation in IRR between study blocks was not associated with inter-household distances, household densities, or coverage (proportion of total households treated). The study confirms the entomological efficacy of the lure-and-kill method to reduce the abundance of this important sand fly vector in treated and untreated homesteads. The outcomes were achieved by low coverage and using only 1-2% of the quantity of insecticide as normally required for IRS, indicating the potential cost-effectiveness of this method. Implications for programmatic deployment of this vector control method are discussed.

Impact and cost-effectiveness of a lethal house lure against malaria transmission in central Côte d'Ivoire: a two-arm, cluster-randomised controlled trial

SummaryBackgroundNew vector control tools are required to sustain the fight against malaria. Lethal house lures, which target mosquitoes as they attempt to enter houses to blood feed, are one approach. Here we evaluated lethal house lures consisting of In2Care (Wageningen, Netherlands) Eave Tubes, which provide point-source insecticide treatments against host-seeking mosquitoes, in combination with house screening, which aims to reduce mosquito entry.MethodsWe did a two-arm, cluster-randomised controlled trial with 40 village-level clusters in central Côte d'Ivoire between Sept 26, 2016, and April 10, 2019. All households received new insecticide-treated nets at universal coverage (one bednet per two people). Suitable households within the clusters assigned to the treatment group were offered screening plus Eave Tubes, with Eave Tubes treated using a 10% wettable powder formulation of the pyrethroid β-cyfluthrin. Because of the nature of the intervention, treatment could not be masked for households and field teams, but all analyses were blinded. The primary endpoint was clinical malaria incidence recorded by active case detection over 2 years in cohorts of children aged 6 months to 10 years. This trial is registered with ISRCTN, ISRCTN18145556.Findings3022 houses received screening plus Eave Tubes, with an average coverage of 70% across the intervention clusters. 1300 eligible children were recruited for active case detection in the control group and 1260 in the intervention group. During the 2-year follow-up period, malaria case incidence was 2·29 per child-year (95% CI 1·97–2·61) in the control group and 1·43 per child-year (1·21–1·65) in the intervention group (hazard ratio 0·62, 95% CI 0·51–0·76; p<0·0001). Cost-effectiveness simulations suggested that screening plus Eave Tubes has a 74·0% chance of representing a cost-effective intervention, compared with existing healthcare activities in Côte d'Ivoire, and is similarly cost-effective to other core vector control interventions across sub-Saharan Africa. No serious adverse events associated with the intervention were reported during follow-up.InterpretationScreening plus Eave Tubes can provide protection against malaria in addition to the effects of insecticide-treated nets, offering potential for a new, cost-effective strategy to supplement existing vector control tools. Additional trials are needed to confirm these initial results and further optimise Eave Tubes and the lethal house lure concept to facilitate adoption.FundingThe Bill & Melinda Gates Foundation.

Controlling Pectinophora gossypiella and Earias insulana in Cotton Filed by the 12th Generations of Trichogramma evanescens Reared in Normal Conditions in the Laboratory

A field trial was done at 2018-2019 cotton seasons to evaluate the parasitoid of Trichogramma evanescens (Westwood) local strain which rearing for 12th generation under normal conditions in the laboratory; compared with the conventional insecticide applications profenofos (organophosphorus compound); for controlling the two pests pink bollworm, Pectinophora gossypiella (Saund.) and spiny bollworm, Earias insulana (Boisd.) on infested cotton green bolls. The real control effect (pest population and infestation reductions percentages) by T. evanescens was determined through the weekly infestation level of green cotton bolls comparing with the control. When T. evanescens was released in comparison with profenofos insecticide treatment, caused more reduction of pink and spiny bollworms infestation when used as 3&5 cards than profenofos comparing with untreated at two seasons, respectively. Pest control by releasing T. evanescens levels was relatively lower as 1 card parasitoid than 3 as well as 5 cards. Also, the infestation reduction was high when this parasitoid was releasing in cotton earlier in the season without any other control agents. The obtained results show that the release of T. evanescens to control pink and spiny bollworms is biologically effective and could be used as an important agent in integrated pest management programs.

ZnFe2O4-chitosan magnetic beads for the removal of chlordimeform by photo-Fenton process under UVC irradiation

A simple protocol was proposed for the preparation of magnetic chitosan beads ZnFe2O4-CS via a co-precipitation method. The use of synthesized magnetic ZnFe2O4-CS beads as catalyst for the heterogeneous photo-Fenton treatment of chlordimeform insecticide (CDM) was evaluated. The photo-Fenton experiments were carried out with different synthesized catalysts by varying the molar ratio Zn/Fe in chitosan beads, the catalyst concentration and pH. Under optimal conditions using 1 g of ZnFe2O4-CS beads with a molar ratio Zn/Fe = 0.35 and at pHinitial = 3, a real wastewater doped with 20 mg L−1 of CDM was treated and complete removal of the insecticide was achieved after 7 min with a total TOC removal after 2 h of treatment. The generated carboxylic acids and ions during the photo-Fenton process were identified and quantified. The stability of the photocatalytic activity of the best catalyst in terms of pollutant removal, ZnFe2O4-CS(0.35) beads with a molar ratio Zn/Fe equal to 0.35, was satisfactory validated by four consecutive cycles. This optimal catalyst was characterized, before and after use, by Scanning Electron Microscopy/Energy Dispersive X-Ray Spectroscopy, X-Ray Powder Diffraction and Vibrating Sample Magnetometry analysis.

Entomopathogenic nematodes and fungi to control Hyalesthes obsoletus (Hemiptera: Auchenorrhyncha: Cixiidae)

Hyalesthes obsoletus Signoret (Hemiptera: Auchenorrhyncha: Cixiidae) is a univoltine, polyphagous planthopper that completes its life cycle, including the subterranean nymph cryptic stage, on herbaceous weeds. In vineyards, it can transmit ‘Candidatus Phytoplasma solani’, an obligate parasitic bacterium associated with bois noir (BN) disease of grapevine, from its host plants to grapevine when occasionally feeding on the latter. The main disease management strategies are based on vector(s) control. Insecticide treatments on grapevine canopy are completely inefficient on H. obsoletus, due to its life cycle. Consequently, control of this planthopper focuses on the nymphs living on the roots of their host plants. Such practices, based on herbicide application and/or weed management, can reduce vector density in the vineyard but can impact the environment or may not be applicable, highlighting the necessity for alternative strategies. In this study, the efficacy of entomopathogenic nematodes (EPNs; Steinernema carpocapsae, S. feltiae, Heterorhabditis bacteriophora) and fungi (EPFs; Beauveria bassiana, Metarhizium anisopliae, Isaria fumosorosea, Lecanicillium muscarium) against H. obsoletus nymphs (EPNs) and adults (EPNs and EPFs) was assessed under laboratory and greenhouse conditions. The majority of examined EPNs and EPFs were able to kill H. obsoletus exhibiting a range of effectiveness. S. carpocapsae (among EPNs) and I. fumosorosea (among EPFs) were found to be the most effective biocontrol agents in all trials carried out. Advantages and limitations of such promising biocontrol agents were discussed. Ecological competency and conditions that can impede or enhance the EPNs and EPFs performance should be investigated to optimize their performance under field conditions.

Organic anion-transporting polypeptides are involved in the elimination of insecticides from the red flour beetle, Tribolium castaneum

Organic anion-transporting polypeptides (OATPs) are integral membrane transporters that mediate cellular uptake of a broad range of substrates in humans. The functions of OATPs in insects are less well investigated and only poorly understood. A large number of compounds potentially toxic to insects are organic anions that include secondary plant and insecticide-derived metabolites. Some insect OATP genes are expressed in metabolic, neuroprotective and excreting tissues, and they are co-expressed together with genes known to be involved in detoxification and excretion. Therefore, a role in the elimination of insecticides has been proposed for OATPs, but experimental proof was pending. The aim of this study was to identify OATPs that affect tolerance to insecticides in the red flour beetle, Tribolium castaneum, a genomic model species and stored product pest. We determined expression profiles of TcOATP genes in different tissues and developmental stages and analyzed RNAi phenotypes. We found that some TcOATP genes had particularly high transcript levels in relevant tissues and that knockdown of TcOATP4-C1 led to sever developmental defects during larval–pupal molt. Then, we exposed the larvae to different chemically unrelated insecticides and analyzed transcript levels and mortalities. Some genes were specifically upregulated in response to insecticide treatment, and mortalities observed after administering certain insecticides were significantly increased when specific TcOATPs were silenced. By applying systemic RNAi in T. castaneum, we provide first evidence that OATPs are involved in the elimination of insecticides and hence may contribute to insecticide resistance, which becomes an increasingly serious problem in agriculture and forestry.Graphic abstract

Evaluate the Effects of Potential Botanical and Conventional Insecticides on the Reproductive and Developmental Aspects of the Pest Agrotis Ipsilion (Lepidoptera: Noctuidae)

Background: Agrotis ipsilon (Hüfn.), is known as a hazardous destructive pest for corn, Zea mays (L.) in Egypt, where it negatively affects corn yield production. Using insecticides continuously were sharply affected the progress of pest development in the field. The frequent uses of chemical pesticides to protect field crops leads to the development of insecticide-resistant strains.&#x0D; Aim: Evaluate the insecticidal effect of four plant extracts with insecticidal properties and a chemical insecticide to control A. ipsilon using biological parameters and fertility life table. Also, to estimate the effects of these insecticidal materials on population growth, developmental progress, and nutritional indices and hence make recommendation of the type of insecticides to be used in Integrated Pest Management Programs.&#x0D; Methodology: 1- Tested materials: (1) chemical insecticide (Chlorfenapyr at 0.5% concentration). (2) Four plant extracts (petroleum ether extracts of both Melia azedarach and Vinca rosea, and alcohol &amp; hexane extracts of Conyza aegyptiaca) at 5% concentration each.&#x0D; 2- Tested insect: Agrotis ipsilon (the egg stage and the 4th larval instar).&#x0D; 3- Bioassay: Eggs and larvae were treated with tested materials separately, then different biological parameters were calculated.&#x0D; 4- Life table parameters: Life table parameters were calculate using the “Age-stage, two-sex life tables” computer program.&#x0D; Results: The obtained data revealed that petroleum ether extracts of V. rosea and M. azedarach were the best antifeedant agents against the 4th larval instar of A. ipsilon. The female longevity was significantly shortened under the effect of both treatments of M. azedarach and Challenger insecticide. All tested materials reflected an effective decline in the female ability for laying eggs, where this effect was obvious in the case of M. azedarach and Challenger insecticide treatments. It was found that the Melia treatment prolonged the incubation and developmental periods for the immature stages leading to a reduction of some life table parameters of A. ipsilon and also increase the generation mean time.&#x0D; Conclusion: The resultant data obtained in this work may help in the usage of plant extracts in the advancement of IPM programs for the greasy cutworm, (Agrotis ipsilon) in Egypt.

Using multilevel models to explore the impact of abiotic and biotic conditions on the efficacy of pirimiphos-methyl against Tenebrio molitor L.

In this study, we utilized a hierarchical multilevel modeling approach to test the hypothesis that the activity of the organophosphate insecticide pirimiphos-methyl against the cosmopolitan serious secondary pest of stored products, the yellow mealworm, Tenebrio molitor (Coleoptera: Tenebrionidae), is affected by temperature, relative humidity (RH), and developmental stage (adults, small larvae, large larvae). Our results showed that as temperature increased from 20 to 25°C, the observed mortality of T. molitor was significantly higher. Furthermore, mortality at 25 °C did not significantly differ from that of 30°C. An ultimate increase at 35°C resulted in the highest mortality rate of T. molitor. However, an increase of RH from 55 to 75% adversely affected the efficacy of pirimiphos-methyl. In our study, it is also shown that the insect developmental stage is a critical feature of pirimiphos-methyl efficacy. Tenebrio molitor adults exhibited significantly higher mortality than larvae. In addition, small larvae showed significantly higher mortality than large larvae. Thus, adult is the most susceptible developmental stage of T. molitor to pirimiphos-methyl treatment. Our results could be useful tools for the management of T. molitor by indicating the optimum combination of temperature and RH that favors the insecticidal treatment against this species. In addition, we expect that the percentage of developmental stages in a whole population of T. molitoraffects the insecticidal efficacy of pirimiphos-methyl.

Abundance and diversity of arthropods in transgenic Bt and non-Bt cotton fields under Indian conditions

Two-year field studies during Kharif 2015 and 2016 were conducted to assess the abundance and diversity of arthropod communities of Bt cotton carrying Cry1Ac and Cry2Ab proteins and non-Bt cotton under sprayed and unsprayed conditions. The experiment was conducted in a randomized block design with four treatments of BG-II and non-Bt cotton. The treatments were non- transgenic cotton with no insecticide treatment, non-transgenic cotton with insecticide treatment, transgenic cotton with no insecticide treatment and transgenic cotton with an insecticide treatment. Arthropod community categorized as target insect pests, sucking insect pests, natural enemies and non-target insects were recorded at different phases of the cotton crop. The sucking insect pests contributed the highest abundance among the total arthropods recorded during the period of study. The abundance and diversity of bollworms that were recorded from non-Bt cotton only suggested effectiveness of BG II cotton against target insect pests. The abundance, species richness and diversity indices with respect to populations of beneficial predaceous arthropods and non-target insects were similar in BG-II and its non-Bt counterpart under unsprayed conditions. On the contrary, these parameters were significantly on the lower side under sprayed regimes for both genotypes. The spray of insecticides in early season against sucking insect pests significantly reduced their abundance and diversity indices in both BGII and non-Bt cotton. As monocropping of BG-II cotton is common on large parts of India, there is a need to monitor the populations of non-target insect species with special emphasis on sucking insect pests on a continuous basis to understand the long-term impact of Bt crops on insect biodiversity for sustainable integrated pest management practices.

Condition of entomophilic fauna on Hyssopus officinalis L. crops depending on ecotope conditions

Species diversity and number of potential pollinator species on H. officinalis seed crops in various ecotopes of farm fields were studied under the influence of “Bi-58 new” insecticide treatment and without preliminary treatment. The presence of 16 insect species belonging to four families of the genus Hymenoptera (Megachilida, Apidae, Halictidae and Vespidae), two families of the Diptera row (Syrphidae and Stratiomyidae) and one family of the genus Lepidoptera (Lycaenalidae) has been identified on the seed crops of H. officinalis. The insecticidal treatment in general did not significantly affect the total number of pollinators, but changed the species composition of pollinators and their number on the field area. As much as possible, the decrease in the prevalence and number of the Megachilida species was affected.

Effect of Imidacloprid and Acephate for Tobacco Thrips (Thysonaptera: Thripidae) Management on Flumioxazin Injured Peanut

ABSTRACT Experiments were conducted in 2016 and 2017 in Stoneville and Starkville Mississippi to determine the impact of different insecticide management options for thrips on herbicide injured peanut. Insecticide treatments included imidacloprid in-furrow at-planting, one or two foliar applications of acephate, and an untreated control with and without an application of flumioxazin. In Stoneville, herbicide applications were made immediately following planting, and in Starkville, applications were made as plants were emerging to maximize herbicide injury. The Stoneville experiment also had an additional factor in which plots were flooded or not flooded to simulate a heavy rainfall in order to maximize herbicide injury and also to give added stress from saturated soils. Thrips counts, thrips injury ratings, plant vigor ratings, plant biomass, width between plant canopies, and yield were recorded. Few interactions were observed, but temporary flooding, herbicide injury, and thrips injury affected peanut growth as measured by biomass and canopy. Imidacloprid was the most consistent insecticide treatment for reducing thrips numbers and injury, but acephate provided some protection. Temporary flooding during the seedling stage, flumioxazin injury, and thrips injury all reduced peanut pod yield. Based on these results, every attempt should be made to minimize early season stress in peanuts including the use of an effective in-furrow insecticide.

Double‐crop soybean management practices for high yield and profitability

AbstractDouble‐crop soybean [Glycine max (L.) Merr.] production in Kentucky involves planting soybean after soft red winter wheat (Triticum aestivum L.) harvest during June and July. Double‐crop soybean yields are lower than full‐season soybean yield planted earlier in the spring. This research aimed to determine the effect of seeding rate, seed treatment, and applying foliar fungicide and insecticide at the R3 growth stage at two planting timings on double‐crop soybean seed yield, yield components, and seed quality. Field trials were established in Princeton, KY, in 2017, 2018, and 2019 after wheat harvest. Planting double‐crop soybean early, after wheat was harvested at 20 to 22% grain moisture, resulted in increased seed yield, seeds ft–2, and protein concentration compared with double‐crop soybean planted later. A seeding rate of 225,000 seeds acre–1 resulted in increased seed yield, seeds ft–2, and seed protein concentration compared with 150,000 seeds acre–1. A seed treatment including fungicides and an insecticide did not impact seed yield, yield components, or seed protein or oil concentration compared with no seed treatment. Applying a prophylactic foliar fungicide and insecticide treatment at the R3 growth stage increased seed yield and seed mass compared with an economic threshold application. In addition, the most intensive management (225,000 seeds acre–1, seed treatment, and prophylactic fungicide and insecticide) yielded ∼25% more than the base treatment and had more seeds ft–2. The results are the first to indicate the potential for intensive management practices to increase double‐crop soybean productivity in Kentucky.

Relative impacts of gypsy moth outbreaks and insecticide treatments on forest resources and ecosystems: An experimental approach

Abstract1. Gypsy moth outbreaks cause severe defoliation in Holarctic forests, both in North America where it is invasive, and in its native range in Eurasia. Defoliation can hamper timber production and impact ecological communities and processes. Aerial insecticide applications are regularly performed in outbreak areas to mitigate economic losses. These operations can be financially costly and harmful to non‐target species and may disrupt species interaction networks. However, replicated studies of the relative impacts of gypsy moth outbreaks and insecticide application on forest growth and animal communities are rare and have yet to be carried out in the species' indigenous range.2. Here, we review the pathways in which gypsy moth outbreaks and the chemical control of these outbreaks affect forest ecosystems. We then present an experimental design established in South Central Germany in early 2019, aiming to study the ecological and economic consequences of gypsy moth eruptions and insecticide application in oak forests. The study's full factorial design comprises forest stands at high and low defoliation risk, either treated with tebufenozide or left unsprayed, within 12 experimental blocks. Measurements of forest growth and structure, tree mortality, gypsy moth density, and composition of lepidopteran, bird, bat, ground beetle, and canopy arthropod communities will be conducted for several years.3. One‐year intensive monitoring of gypsy moth populations and damage across the selected sites showed substantial differences in population density between plots at high and low defoliation risk and high efficacy of tebufenozide in suppressing gypsy moth populations in treated plots. In the first year of the experiment, gypsy moth density and defoliation in predicted outbreak plots differed strongly, confirming the importance of using many replicates and blocking to control spatial heterogeneity. The experiment will be running continuously during the coming years to produce short‐ and medium‐term economic and ecological data to improve our understanding and management of gypsy moth outbreaks.

Identification of Two Additional Behaviorally Active Gland Constituents of Female Diatraea saccharalis (Fabricius) (Lepidoptera: Crambidae)

The sugarcane borer, Diatraea saccharalis (Fabricius), is the major pest insect attacking sugarcane crops in Brazil. Population control by insecticide treatment is not efficient due to simultaneous presence of all developmental stages of the insect throughout the year, and alternative control methods are needed. Two female-produced sex pheromone components, (Z,E)-hexadeca-9,11-dienal and (Z)- hexadec-11-enal, have previously been reported to elicit antennal activity and behavioral response of males in flight tunnel experiments. However, the attractiveness of these compounds in field tests has been very low. In this study, two additional female-produced compounds in D. saccharalis eliciting consistent antennal response in males were identified as (Z)-hexadec-9-enal and hexadecanal. In flight tunnel assays, the behavioral response to a quaternary blend was significantly higher when compared with the previously identified binary blend. Subtracting (Z)-hexadec-9-enal or hexadecanal from the full blend did not reduce attraction, indicating redundancy in the communication channel. We conclude that additional compounds are part of the sex pheromone of D. saccharalis, which may improve the efficiency of trap lures for monitoring of this pest.

Bio-efficacy of newer insecticides against okra shoot and fruit borer, Earias vittella fabricius

Different newer chemical insecticides were evaluated for their effectiveness against okra shoot and fruit borer, Earias vittella during Kharif 2019. The experiment was conducted at the Instructional Farm, Department of Agricultural Entomology, Mahatma Phule Krishi Vidhyapeeth, Rahuri. Three foliar applications of each insecticide treatment were given at an interval of 10 days, initiating first application when pest reached to ETL. The result of the study revealed that the treatment of combination insecticide viz. flubendiamide 90 SC + deltamethrin 60 SC (36+24g a.i./ha) was proved to be the most effective with lowest larval population (0.81 larva/plant), least fruit infestation (4.5%) and highest fruit yield (9.64 t/ha) among all the treatments.

Evaluation of Insecticidal Seed Treatments and Foliar Applied Insecticides Against Rice Billbug in Louisiana Row Rice, 2020

Evaluation of Insecticidal Seed Treatments and Foliar Applied Insecticides Against Rice Billbug in Louisiana Row Rice, 2020

Economic Returns on Insecticidal Seed Treatment Combinations in Louisiana Rice

Economic Returns on Insecticidal Seed Treatment Combinations in Louisiana Rice

Вплив систем землеробства та обробітку грунту на основних шкідників пшениці озимої

The intensification of agriculture has a number of negative consequences, and the conservation of land resources during their active exploitation is an important problem nowadays, paying attention to the ever-growing food increase necessity. The world organic farming practice shows that we are very limited in the agricultural crops pests regulation. Artificial natural enemies increasing and the landscape altering to enhance biological control are not popular solutions and have contradictory efficiency. Monitoring of the winter wheat entomocomplex number was carried out according to conventional methods. It was performed at 48 plots, where 12 variants were placed in 4 replicates during 2019 - 2021. The article contains the research results show that farming systems have a significant impact on the main winter wheat pests, and that they can control the ecological system at the level of the economic harm threshold. The way the soil was treated did not change the number of Schizaphis graminum R., Eurygaster integriceps P., and Oulema melanopus L., while chisel cultivation of 20–22 centimeters provided regulation of Anisoplia austriaca H. and Haplothrips tritici Kurd number way more below the dangerous level for years of observation. Whenever the soil was treated to a depth of 6-8 centimeters, the number of these pests increased significantly and varied from 2.0 ind./m2 to 14.4 ind./m2. Therefore, this data shows the efficiency of insecticidal winter wheat treatments multiplicity reduction in an ecological system without the risk of crop loss due to the plant damaged by pests in combination with chisel soil cultivation.