Abstract
Tea plant is an economic plant, its cultivation has been infected by many agricultural pests, one of which is Ectropis obliqua. A large number of Ectropis obliqua has caused serious harm to many tea areas in China. To elucidate the immune defense and detoxification mechanism of E. obliqua against pyrethroid insecticides, RNA-sequencing was used to analyze transcriptomic data of E. obliqua at different time periods after deltamethrin treatment. Using Illumina sequencing platform, approximately 51,688 clean readings (including 78.23 Gb of sequence data) were obtained from E. obliqua at different time after deltamethrin treatment. These fragments were assembled into 30,601 Unigene with an average length of 1030.43 bp, of which N50 of 1689 bp. Compared with the control group, 1535 differentially expressed genes were found, including 1427 genes up-regulated and 108 genes down regulated. Gene ontology analysis indicated that differentially expressed genes accounted for a high proportion in cell process, single organism process, metabolic process etc. KEGG annotation indicated that differential genes were mainly enriched in basic metabolism needed for life activities and glutathione metabolism and other pathways. The verification by qRT-PCR indicated that the transcriptome data is reliable and can be used for follow-up analysis. Interestingly, it was found that EoGST968 could strongly respond to insecticide stimulation, and the recombinant EoGST968 expressed in prokaryotic cells could catalyze the binding of reduced glutathione (GSH) to1-chloro-2,4-dinitrobenzene (CDNB).
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