Indonesian Ornamental Crops Research Institute Research Articles

Efficacy of some botanical insecticides against <em>Aphis gossypii</em> Glover (Hemiptera: Aphididae) on chrysanthemum

An evaluation of various botanical insecticides to control Aphis gossypii and its impact on aphid population dynamics on chrysanthemum plants was investigated. In order to control A. gossypii on chrysanthemum, the effectiveness of several botanical insecticides extracted from Melia azedarach, Toona sinensis, and Chrysanthemum cinerariaefolium was investigated in the current study. The research was carried out in the experimental field of the Indonesian Ornamental Crops Research Institute under plastic house conditions. Five concentrations, i.e., 1.5, 2.0, 2.5, 3.0, and 3.5 g/L of three plant extracts, M. azedarach, T. sinensis, and C. cinerariaefolium, were sprayed on chrysanthemum cultivar White Fiji after 28 to 84 days after planting. A. gossypii had a dynamic population that changed according to the plant’s developmental stages. In vegetative growth, the alate adult and nymphal stages were dominant, and the population of nymphs increased along the plant ages. The insect colonized young leaf surfaces in the terminal apices. During the reproductive stages, the population of alate adults diminished, and the distribution of the insect extended to mature, old leaves, flower buds, and bloomed flowers. The application of several botanical insecticides revealed various responses of aphid populations. Among the tested insecticides, C. cinerariaefolium extract at 3.0 and 3.5 g/L demonstrated the highest average percentage efficacy (76 and 72%) and was the most consistent in suppressing the population. The results of this study indicate the potential efficacy of botanical insecticides against A. gossypii suggesting a different approach to efficient and environment-friendly chrysanthemum pest management.

Effects of Several Botanical Insecticides Applied in Different Periods to Control Aphids (Macrosiphoniella sanborni Gillete) on Chrysanthemum

Pest management in the agricultural process has faced worldwide economic and ecological issues related to chemicals usage. Botanical insecticides have long been considered promising alternatives to control pests because botanicals reputedly pose a minor threat to the environment and human health. Some studies have evaluated several botanical insecticides to control aphids in chrysanthemum. The research was conducted under plastic house conditions at the Indonesian Ornamental Crops Research Institute from January to December 2018. Leaf extract of T. sinensis, T. diversifolia and A. indica at the concentration of 3 and 3.5 g/l, processed oil of C. nardus at the concentration of 2.5 and 5 g/l and synthetic insecticide Imidacloprid 1 g/l were sprayed in the morning or late afternoon. The results showed that aphid attacks severity and percentage of attacked plants were lower when the insecticides were applied in the late afternoon. Slightly softer than synthetic chemicals, the leaf extract of T. sinensis at 3 g/l suppressed the aphid development more effective for about 55.93% than other botanicals treatments. On reproductive properties, the leaf extract of T. sinensis at 3 and 3 g/l and T. diversifolia at 3 g/l induced a higher percentage of open flower for more than 65% than synthetic insecticides, Imidacloprid.

Evaluation of Culture Media for In Vitro Conservation of Gladiolus Cultivars

In vitro conservation is recognized as one promising tool in conserving plant genetic resources like gladiolus when grown in the tropics. The study was conducted at the Indonesian Ornamental Crops Research Institute (IOCRI) from February 2016 to August 2018. A complete factorial experiment was set up to establish the combination of gladiolus cultivars and media compositions treatments for in vitro conservation. The results showed that the trends of plantlet survival rate and viability in every 4 months’ observation of both cultivars were similar. The highest plantlet death rate was detected during 12 – 16 months. After 24 months of storage, the differences in plantlet survival and viability were observed. Gladiolus ‘Nafa’ had higher plantlet survivals and viability than ‘Kaifa’. In respect to nutrient and sucrose concentration, full nutrient strength preserved 5% higher plantlet survival and viability than ½ MS. While media contained sucrose 90 g/l also supported plantlet survival and viability for more than 20% and 17%, respectively than lower sucrose media.

Efficacy of Selected Plant Extracts to Control Leaf Miner (Lyriomyza spp.) in Chrysanthemum

The use of eco-friendly methods in controlling pest and diseases has become an increasing concern in production system in many economically important crops, including ornamentals. The application of plant extract that has an insecticidal effect is considered as one promising alternative in reducing the negative effects of synthetic pesticides. The research was conducted to assess selected plant extracts in several concentrations against leaf miner (Lyriomyza spp.) in chrysanthemum. The experiment was carried out from January to December 2017 under plastic house conditions at the Indonesian Ornamental Crops Research Institute (IOCRI). The extracted organ of insecticidal plants regularly sprayed into chrysanthemum plants and compared with water as the controls. The results showed that the application of insecticidal plants extracts reduced attack intensity and incidence from water treatment (control). Certain treatment combinations, Chinese mahogany leaf extract at the concentration of 0.25%, C. pyrethrum petal 0.15 and 0.30%, and chinaberry leaf at 0.3, 0.35% even had 0.9-3.13% lesser attack incidences than commercial botanical insecticide Neem Plus. The respected treatments also supressed more than 62% leaf miner attacks and induced the increase of marketable flowers.

Evaluation of Cladosporium sp. Mycoparacites as Biocontrol Agents of White Rust Disease on Chrysanthemum

Cladosporium is a promising mycoparasite to control white rust disease in chrysanthemum. The research was conducted to evaluate several collected Cladosporium isolates against P. horiana, their parasitism mechanism and population dynamic in a production cycle. The experiment was carried out at the Indonesian Ornamental Crops Research Institute (IOCRI) from January to December 2015. Ten Cladosporium isolates with two synthetic fungicides and one negative control were designedly sprayed on chrysanthemum cv White Fiji. The results showed that Cladosporium isolates gave varied suppression to white rust intensity. Isolates HNC, DC, SGC and UC had higher parasitism and suppression intensities than other isolates and their effectiveness was the same as synthetic fungicides. Longer flower vase life was produced from Cladosporium and synthetic fungicide treated plants, while flower stalk and diameter were less affected by the Cladosporium applications. The population dynamic of Cladosporium was accordingly responded with the rust intensity in Cladosporium-treated plants. The parasitizing mechanism of Cladosporium to P. horiana was through teliospores envelopment resulted in the changes of teliospores morphology.

Phenotypic performance of six interspecific carnation genotypes obtained from hybridization of Dianthus caryophyllus “Liberty” x Dianthus chinensis “SK 11-1”

Interspecific hybridization can be an effective strategy in ornamental plant breeding. Interspecific hybridization in the genus Dianthus is relatively easy to do. Therefore, the introduction of good character carriers from other Dianthus species into carnations will give prospect. The objective of this study was to produce new type interspecific carnation genotypes. The experiment was conducted at the Indonesian Ornamental Crops Research Institute experiment station in Cipanas – Cianjur, from November 2014 – July 2015. The experiment was conducted with an experimental method using randomized block design (RBD). The treatment consisted of six genotypes of interspecific carnation, female parent “Liberty” and male parent “SK 11-1” with four replications. The result showed that the flower color of the purplish red and red interspecific genotypes was different from the female parent yellow-flowered. The character of flower type, petal edges and leaf texture of the six progeny genotypes resemble male parent, while the flower shape character of the six interspecific carnation genotypes resemble female parent. Genotype D1 had a combination of the character of plant height, stem diameter and diameter of the appropriate flower as carnations of cut flowers, while genotype D5 had the character of plant height, the number of flowers blooming at one time, the diameter of the flower, the freshness of the flower suitable as garden carnations or pots.

Combined Application of Bio-PF and Synthetic Fungicide Suppress Soil Borne Disease Caused by Cylindrocladium sp. in Leather Leaf

An evaluation of formulated Bio-PF with the active ingredient of Pseudomonas fluorescens to reduce synthetic fungicide usage in controlling soil borne disease in leather leaf fern under different shelters was studied. The research was conducted at the experimental field of the Indonesian Ornamental Crops Research Institute (IOCRI) from January to December 2015. Different concentrations of Bio-PF were singly and/nor alternately applied with two different synthetic fungicides were applied on leather leaf plants under UV plastic + 70% black-net and 70% black-net shelters. The results showed that the average disease intensity was higher in 70% black-net shelter. Weekly alternate-application of Bio-PF 5 + Carbendazim & Mancozeb and single Carbendazim & Mancozeb gave lower disease intensity than other treatments with the highest percentage of suppression. These two treatments also improved yield in terms of number and proportion of the preferred grade of harvested leaves. Combined Bio-PF 5 + Carbendazim & Mancozeb gave longer vase life and the application reduced synthetic fungicide usage in controlling the respected disease. In term of synthetic fungicides, Carbendazim & Mancozeb gave more consistent effects on disease suppression, plant growth and foliage production than Asilbensolar & Mancozeb.

Genetic variability of some Lilium longiflorum cultivars in Indonesia

Lilium longiflorum has various local cultivars dispersed throughout Indonesia. The objective of this study was to evaluate vegetative and floral traits and to observe genetic variability of those traits. The evaluation was conducted in a field trial at the Indonesian Ornamental Crops Research Institute (IOCRI) during 2015-2016. The experiment was arranged by randomized complete block design with three replications. Seven cultivars of Lilium longiflorum (‘Cipanas’, ‘Sukabumi’, ‘Parompong’, ‘Berastagi’, ‘Bali’, ‘Tomohon’ and ‘Japan’) were collected and their vegetative and floral traits were evaluated. The results showed that ‘Cipanas’, ‘Sukabumi’ and ‘Bali’ had the earliest flowering time (FT) (80-86 days) and ‘Japan’ showed the latest FT. ‘Berastagi’ produced maximum flower diameter (FD) and medium FT and plant height (PH). All characters except FD exhibited narrow genetic coefficient of variation (GCV) but wide phenotypic coefficient of variation (PCV). High heritability value (HV) estimation and high genetic advance (GA) estimation were observed for characters including PH, number of leaves, stem diameter, FT and number of flowers. FD trait was recorded and showed high values of GCV and PCV, high HV but low GA. Selection on the basis of this trait would be more effective on the early generations. Consequently, direct selection of local L. longiflorum in IOCRI will be based on FD improvement. Nevertheless, there were no correlation between FD and others traits.

Effect of Re-Using Vernalization Bulbs on Growth and Development of Lilies

Purpose: Selecting bulbs based on their quality as planting materials is a critical point in obtaining high qualified growth and development of lilies, however published reports in re-using bulbs on cultivation of lilies were still limited. Objective of the research was to reveal effect of re-using vernalization bulbs on growth and development of lily. The research was conducted in plastic house of Segunung Experimental Gerden, Indonesian Ornamental Crops Research Institute. Research Method: Materials used in the study were lily bulbs harvested from the third cultivation period of Asiatic lily, Lilium ‘Delina’, L. ‘Liana’, L. longiflorum and L. ‘Renata’; two bulb sizes of them were grade A and B. The experiment was arranged in split plot design with three replications. Main plot was two bulb sizes of the grade A and B; while sub-plot was five lily varieties of Asiatic, Delina, Liana, Longiflorum and Renata. Findings: Results of the study revealed that re-utilizing lily bulbs harvested from third cultivation period still had high potential in obtaining better growth and development of lily both vegetatively and generatively. The such bulbs kept resulting in optimal growth performances in all lilies tested. Higher bulb sizes produced better lily performances vegetatively and generatively; grade A > grade B in the most varieables observed, while lily varieties indicated varied responses in all variables observed. The best variety of lily growth and preformances vegetatively was showed by Longiflorum, however better generative growth and performances was noted on Liana. While in interaction effect, grade A of Delina gave better results in initial influorescent formation after 71.3 days after culture with 102.3 days initial flower buds opened, 100% plants flowered, 3 flower buds per plant, 18.7 cm flower bud diameter and 45mm bulb diameter. The results of the study gave evidents that re-utilizing lily bulbs derived from third cultivation period still could be applied to produce better quality and quantity of lily flowers. Limitations: The limitations of the research are apparent to the fact that the research was only focused on re-utilization of lily bulbs harvested from third cultivation period. Originality/ Value: Results of the research can be used as important considerations for growers and farmers on producing maximal lily flower on fulfilling market and customer demand under re-utilization of lily bulbs.

Evaluation of Biological and Chemical Fungicides to Control White Rust in Chrysanthemum Grown Under Open Condition

The effort to reduce chemicals has become an important issue in floriculture agribusiness. The chemicals included fungicides for white rust control in chrysanthemum. The combined application of synthetic fungicide with biological agent and botanical fungicide were expected not only to reduce synthetic fungicide application, but also suppressed the white rust attacks. The research was conducted at the Indonesian Ornamental Crops Research Institute (IOCRI). A factorial randomized complete blocked experiment was designed to facilitate combined applications of hexaconazol-50 (SFH-50), antagonist microbe Corynebacterium sp. (Cor-5) and formulated botanical fungicide CEES 50EC (ES-50). The results showed that the combined SFH-50 with Cor-5 and ES-50 merely affected the disease intensity, yet less on white rust incidence, period of disease emergence, plant growth, flower production and quality. All combined applications of SFH-50 with Cor-5 and ES-50 showed lower disease intensity than single SFH-50. Higher suppressions were detected on 1 ml/l SFH-50 + 5 ml/l Cor-5-3 and 1 ml/l SFH-50 + 2 ml/l ES-50-3. The use of antagonist microbe and botanical fungicide singly or in combination with synthetic fungicide could reduce with the higher affectivity than single synthetic fungicide application.

Effects of Planting Density and Concentration of NPK Fertilizer on the Growth of Potted Chrysanthemum (Chrysanthemum morifolium Ramat)

Chrysanthemum is an ornamental plant that people likes, grows the whole year, and hashigh economic value. This study was aimed at assessing the effects of planting density andconcentration ofNPK fertilizer on the growth of potted chrysanthemum. The study was conductedin a plastic house at the Indonesian Ornamental Crops Research Institute (IOCRI), Cipanas,Cianjur, West Java from March to June 2017. Shoot cuttings of ±7 cm of potted chrysanthemum ofAvanthe Agrihorti cultivar were used. A completely randomized design in a factorial pattern withfactors was used. The first factor was planting density and the second factor was concentrationofNPK (16:16:16) fertilizer. The planting density consisted of 5, 6, and 7 shoot cuttings andconcentrationof NPK fertilizer were 150, 200, 250, and 300 ppm. Results showed that plantingdensity significantly affected stem diameter, number of buds, and width of canopy. No effectconcentrationof fertilizer was found on all variables.Keywords: potted chrysanthemum, planting density, NPK fertilizer

The Phenotypic Performances of Gerbera Local Bali X Rubby Red Hybrids

Gerbera is one of important cut flowers in floriculture industries. It can substitute of chrysanthemum. The novelty of cultivar and superiority of characteristic of gerbera are necessary in the floriculture markets. New cultivars can be created through hybridization between cultivars. This study aimed to find out the phenotypic performances of gerbera hybrids of Local Bali x Rubby Red. The research was conducted in Indonesian Ornamental Crops Research Institute, West Java, Indonesia from January 2014 to September 2015. Eight progenies of F1 hybrids and their parents (Local Bali and Rubby Red) were used as materials. In the hybridization process Local Bali and Rubby Red were used as female and male parents respectively. Genetic variabilities of Gerbera were achieved through this hybridization. Gerbera progeny no. 20.009 had the longest inflorescence diameter, the biggest outer sepal and the highest number of sepals compared to the others. The flower vase life of this progeny was not significantly difference to the male parent. Qualitative characters observation showed that the hybrids were classified into two groups based on flower type, four groups of outer ray florets and two-disc groups of petal color.

EFFECTS OF TYPES OF MEDIA AND NPK FERTILIZER ON THE ROOTING CAPACITY OF CHRYSANTHEMUM CUTTINGS

Physiological status of chrysanthemum cuttings during rooting process highly influences plant growth and flower production. Amongst other aspects, rooting capacity of the cuttings is the most important. Rooting capacity is influenced by environmental conditions such as growing medium and nutrition. The study was aimed to analyze the effects of different types of media, fertilizer concentration, and frequency of fertilizer application on the rooting capacity of chrysanthemum cuttings. The experiment was conducted at the Segunung Experimental Station of the Indonesian Ornamental Crops Research Institute from February to May 2005. The treatments consisted of four different rooting media, i.e. carbonized rice husk, coco peat, perlite, and mixture of perlite and vermiculite (1:1 v/v); two concentrations of NPK (25:7:7) fertilizers, i.e. 1.75 and 2.5 g 1-l; and four frequencies of fertilizer application, i.e. every 1 day, 2 days, 3 days, and 4 days. The experiment was designed in a completely randomized block with four replications. Parameters observed were rooting capacity and cutting performances such as cutting height, cutting diameter, number of leaves, number of visible roots, and root length at 16 days after planting. The results showed that chrysanthemum cuttings rooted in carbonized rice husk had better performance than those rooted in coco peat, vermiculite, and mixture of perlite + vermiculite. Higher concentration of fertilizer solution up to 2.5 g l-1 also gave significant effect on the root formation and further root growth of cuttings. However, the rooting capacity of cuttings was not affected by the frequency of fertilizer application. This study implied that carbonized rice husk growing medium supplemented with NPK (2.5 g l-l) every 4 days during rooting period is the choice for traditional chrysanthemum growers to increase the perform-ance and quality of the cut flowers.

EFFECTS OF TYPES OF MEDIA AND NPK FERTILIZER ON THE ROOTING CAPACITY OF CHRYSANTHEMUM CUTTINGS

Physiological status of chrysanthemum cuttings during rooting process highly influences plant growth and flower production. Amongst other aspects, rooting capacity of the cuttings is the most important. Rooting capacity is influenced by environmental conditions such as growing medium and nutrition. The study was aimed to analyze the effects of different types of media, fertilizer concentration, and frequency of fertilizer application on the rooting capacity of chrysanthemum cuttings. The experiment was conducted at the Segunung Experimental Station of the Indonesian Ornamental Crops Research Institute from February to May 2005. The treatments consisted of four different rooting media, i.e. carbonized rice husk, coco peat, perlite, and mixture of perlite and vermiculite (1:1 v/v); two concentrations of NPK (25:7:7) fertilizers, i.e. 1.75 and 2.5 g 1-l; and four frequencies of fertilizer application, i.e. every 1 day, 2 days, 3 days, and 4 days. The experiment was designed in a completely randomized block with four replications. Parameters observed were rooting capacity and cutting performances such as cutting height, cutting diameter, number of leaves, number of visible roots, and root length at 16 days after planting. The results showed that chrysanthemum cuttings rooted in carbonized rice husk had better performance than those rooted in coco peat, vermiculite, and mixture of perlite + vermiculite. Higher concentration of fertilizer solution up to 2.5 g l-1 also gave significant effect on the root formation and further root growth of cuttings. However, the rooting capacity of cuttings was not affected by the frequency of fertilizer application. This study implied that carbonized rice husk growing medium supplemented with NPK (2.5 g l-l) every 4 days during rooting period is the choice for traditional chrysanthemum growers to increase the perform-ance and quality of the cut flowers.

Respon 10 Varietas Krisan (Dendranthema grandiflora Tzelev) terhadap Dua Aplikasi Pemupukan di Lahan Terbuka

ABSTRACT<br /><br />Cultivation of the chrysanthemum is usually carried out under the plastic-house to produce high quality flowers, but in some areas such as in Karo (North Sumatera), farmers have grown the plant in the open field using selected varieties for the same purpose. The study was aimed to assess responses of ten varieties of chrysanthemum toward two applications methods of fertilizer which was carried out in open-field at Segunung Experimental Garden, Indonesian Ornamental Crops Research Institute (IOCRI) from July till December 2011. The experiment was arranged in a split-plot design with three replications. The main plot was ten chrysanthemum varieties of Besar Kuning, Merah Hati and Sakuntala; Besar Bandung, Berlian Putih, Matahari Kuning, Mata Kucing, Mata Dewa, Swarna Kencana and Puspita Nusantara. The subplot were two fertilizer applications methods, Karo and Balithi. Karo: manure 100 kg m-2 and 50 kg m-2 (in month 2), urea 30 g m-2 (in month 3) and Balithi: manure 20 ton ha-1, urea 200 kg ha-1, 350 kg KCl ha-1 dan 300 kg SP-36 ha-1, supplementary 1.5 g urea m-2, 6 g KNO3 dan 6 g SP-36 m-2. Both methods of applications can be applied to open field cultivation. Method of fertilizer application significantly affected stem diameter, intensity of disease infection and the time of flower initiation. The Balithi’s fertilizer application significantly reduced the intensity of rust disease on chrysanthemum, while Karo’s fertilizer application significantly increased stem diameter.<br /><br />Keywords: Chrysanthemum, open-field, two fertilization application, stem diameter, rust disease<br /><br />

Keragaan dan Keragaman Genetik Karakter Agronomi Galur Mutan Putatif Gandum Generasi M5

The cultivation of wheat in Indonesia especially at medium-low altitude faces problems such as high temperatures whicheffect negativelyon the yield. The aims of this research was to obtain informations on performance, genetic variability and heritability of agronomic characters of wheat putative mutant lines on M5 generation. Genotypes evaluated in the study consisted of 98 putative mutant lines of M5 generation and six varieties as checks, namely Dewata, Selayar, Oasis, Rabe, Kasifbey, and Basribey. The research was conducted at Research Station of IOCRI (Indonesian Ornamental Crops Research Institute), Cipanas. Genotypes were arranged in an augmented, randomized block design. Result of the experiment showed that high heritability estimates were observed for plant height (83.03%), tiller number (61.75%), days to flowering (85.41%), days to maturity (67.45%), spike length (70.25%), spikelet number (65.08%), unfilled grain number (71.49%), seed number per spike (75.16%), seed weight per spike (81.69%), and seed weight per plant (74.21%). The estimated genotypic coefficient of variation (GCV) were high for unfilled grain number(26.35%) and seed weight per plant (18.35%). Based on seed weight per plant, 30 best putative mutant lines were selected.<br />Keywords: Augmented design, heritability, Triticum aestivum

Improvement of Selected Induction Culture Media on Callus Induction in Anther Culture of Anthurium and a Histological Study on its Callus Formation

Improvement of selected induction culture media on callus induction in anther culture of anthurium and a histologicalstudy on its callus formation were studied at the tissue culture laboratory of the Indonesian Ornamental CropsResearch Institute from February to October 2008. The objectives of the study were to optimize selected media forcallus formation, reveal cell origin of callus derived from anther culture and shoot formation process. Selectedmedia improved in the study were 1) MMS-TBN containing 0,5 mg/l TDZ, 1,0 mg/l BAP and 0,01 mg/l NAA (Winartomedium, WM) and 2) MMS III supplemented with 1,5 mg/l TDZ, 0,75 mg/l BAP and 0,02 mg/l NAA (Winarto andRachmawati medium, WRM). Improvement treatments were carried out by omission and application of 2,4-D in 0.5mg/l and reduction of medium strength of full, half, quarter, one eighth, one sixteenth, and zero strength. Afactorial experiment was arranged using a randomized complete block design with four replications. Results ofthis study indicated that the highest callus induction was clearly established in WRM. The medium stimulatedpotential growth of anther (PGA) up to 81% with 49% of percentage of anther regeneration (PAR) and 2.7 number ofcallus formed per replication (NCF). Significant improvement in callus formation was also recorded by reduction ofmedium strength of WRM to one eighth compared to others. The reduction induced PGA up to 58% with 29% of PARand 1.8 NCF. From histological studies it was well recognized that regenerated callus on half anthers cultured wasoriginated from middle layer cells of anther wall. The morphogenic response of anther wall cells caused primarilyon no androgenesis effect in microspore cells.

Micro Propagation of Several Potted Anthurium Accessions Using Spathe Explants

The introduction of new varieties and production system of anthuriums has faced some problems due to the lowmultiplication rates in conventional vegetative and genotypic alteration problem in reproductive propagation. Sincethen, in vitro propagation technique became important to be investigated. The research was carried out fromSeptember 2006 to August 2007 at the Indonesian Ornamental Crops Research Institute. A complete factorialexperiment was designed to accomplish two chronological in vitro activities. The first step dealt with differentincubation sites, i.e. dark and light conditions for callus induction of three potted anthurium accessions, namelyclone no. SM. 001, cv. Alphine and cv. Bonito. The best callus obtained from the incubation treatment were then,transferred into defined media with different formulations to get free-callus plantlets in the second steps. Theresults showed that more progressive callus development from spathe explant was detected on the media ofmodified Nitsch and Nitsch + 1 mg/l 2,D + 1 mg/l Kin + 1 mg/l BA stored at dark after 60 days incubation. After 45days transferring the callus in the same media on light conditions, torpedo-shaped callus was detected. Completeplanlets were obtained after 75 days reculturing the buds in Nitsch and Nitsch medium containing 2,4 D withvarious BA concentrations. Clone SM. 001 and cv. Bonito showed higher number of visible shoot and rootdevelopments compared to cv. Alphine. In general, better shoot and root developments and higher planlets survivedafter acclimatization were observed on the BA concentrations of 2 and 3 mg/l.

KARAKTERISTIK PSEUDOMONAS VIRIDIFLAVA: PENYEBAB PENYAKIT BUSUK LUNAK DAN EVALUASI VIRULENSINYA PADA KLON ANGGREK PHALAENOPSIS

The objectives of this research were to determine the typical symptoms and isolates chararteristics of Pseudomonas viridiflava as the causal agent of soft rot bacterial disease and to find out orchid Phalaenopsis clones resistant to the disease. The scope of this study included exploration and isolation of the pathogens, pathogenicity, biochemical and LOPAT tests, also screening the Phalaenopsis F1 clones resistant to the pathogen. The results showed that isolates Vd-6, Ph-7 and Ph-18 obtained from Vanda (in Bandung Nursery) and Phalaenopsis orchids respectively, in glass house of Indonesian Ornamental Crops Research Institute, were identified as P. viridiflava and highly virulent on tested orchid plants. Typical symptoms were soft rots characterized by moist and watery decay of the whole leaves, which were initiated as small water-soaked lesions and enlarged rapidly to the entire leaves. Based on biochemical and LOPAT tests, the causal organisms were fluorescent green colonies in King’s B medium, not producing levan compound and arginine hydrolase reaction. Pectinolytic activities on potato and tobacco hypersensibility were also positive. According to evaluation test, 10 F1 accecions Phalaenopsis clones (VL 231, VL 232, VL 233, and VL 240; M22.213 and M22.223; 599.201, 599.205, 599.215 and 599.243) were resistant to that pathogen.

ELIMINATION OF Cucumber Mozaic Virus (CMV) FROM A RANGE OF CHRYSANTHEMUM CULTIVARS THROUGH MERISTEM CULTURE FOLLOWING HEAT TREATMENT

Cucumber Mosaic Virus (CMV) is one of pathogenic viruses that commonly found and has caused significant losses in many chrysanthemum growers in Indonesia. Efforts have been made to get healthy plant source through eliminating virus from infected plants and one of the promising methods was the combination of thermotherapy and meristem culture. The research was conducted to find out the effect of meristem culture following heat treatments on the existence of CMV in infected chrysanthemum plantlets. The experiment was carried out in the laboratory of tissue culture and virology at The Indonesian Ornamental Crops Research Institute (IOCRI) from August 2007 until June 2008. A complete factorial with ten replications was designed to accomplish the combination of two factors. The first factor was three chrysanthemum cultivars, namely Stroika, Dewi Sartika and White Fiji, while the second dealt with the durations of heat treatment i.e. one, two and three weeks. The results showed that plantlet survival decreased, yet improved plantlet performance by faster bud initiation with lengthened heat duration. The percentage of virus-free planlets also increased along with the duration of treatment and three weeks heat treatment followed by meristem culture effectively eliminated CMV from infected planlets.