Indian Earthworm Research Articles

Evaluation of Anthelmintic Activities of Persea americana and Musa sikkimensis

The goal was to know about the anthelmintic properties of the leaves of P. americana and peels of M. sikkimensis. An ethanolic extract from the leaves of P. americana and aqueous extract of M. sikkimensis was used to screen for phytochemicals and analyse the anthelmintic activity on Indian earthworm Pheretima posthuma to determine its therapeutic capabilities. The experiment was carried out by preparing standard drug solution (Albendazole), P. americana leaf extract and M. sikkimensis peel extract in a concentration range of 5mg/ml, 10mg/ml and 20mg/ml respectively for each. The earthworms were placed in a beaker with solutions of varying concentrations. The time of paralysis and death was noted and plotted in a graph. From the results, it was found that P. americana and M. sikkimensis extract caused paralysis in less time in a dose-dependent manner, as well as the death was caused much earlier compared to the control group. This suggests that P. americana and M. sikkimensis extract exhibited strong anthelmintic activity. Furthermore, a link has been found between anthelmintic activity and high levels of flavonoids, terpenoids and tannins. Flavonoids, tannins and terpenoids found in various extracts have been proven to have anthelmintic properties in earlier investigations. Persea americana have been reported to have alkaloids, flavonoids, glycosides, saponins, steroids, tannins, proteins, and Musa sikkimensis have been reported to have alkaloids, glycosides, saponins, steroids, terpenoids, tannins, proteins. These results lead to the conclusion that P. americana and M. sikkimensis extract may possess anthelmintic activity. Keywords: Anthelmintic activity, Helminth, Musa, Avocado

Comparative Study of Herbal drugs and Synthetic drugs used for treatment of Intestinal Worm Infestation

The purpose of this study was to compare the herbal drugs which are used traditionally with commonly used synthetic drugs for the treatment of intestinal worm infestation. The experiment was conducted using adult Indian earthworms (Pheretima Posthuma) owing to their anatomical and physiological similarity to human intestinal parasites, making it convenient for the evaluation of anthelmintic compounds in a laboratory setting. As a control sample and preparation of samples normal saline solution was used. Various concentrations of both herbal and synthetic drugs were tested and result was obtained in the terms of time of paralysis and time of death in minutes. All the drugs show different time of paralysis and death of worms. In this study we took herbal powders without any extraction process or chemical process done on it.

Evaluation of Anthelmintic Activity of Caesalpinia Pulcherrima Leaf Extract by In-Silico and In-Vitro Studies

The Greek term for "helminthes" means "worm." Human-infecting parasites fall into one of two categories: keepsakes or heirlooms. Heirlooms are parasites that have been passed down from African ancestors, while souvenirs are parasites that humans have picked up from animals through migration, evolution, and agricultural activities. These helminthic infections are the most prevalent human infectious agents in poor nations. The helminthic parasite affects around 2 billion people worldwide, or more than 25% of the total population. It is a big problem in developing nations, particularly for young people. Therefore, the goal of the current study was to extract the plant's active ingredients and test the crude extract's anti-helminthic properties. In this study, we worked on the ceasalpeniapulcherima plant with a focus on natural remedies for the treatment of helmintiasis. The chief scientist of Sri Konda Laxman Telangana State Horticulture University, Floriculture Research Station, Rajndranagar, authenticated the true nature of the plant. Water and ethanol were utilized in the extraction procedure. To find out what chemicals are in the alcoholic extract, a screening test is performed on it. The flavonoid test on the extract came out positive. Using Albendazole as the prescribed medication, the anthelmintic action was carried out on Indian earthworms. In Indian earth worms, we found that alcoholic extract exhibits good antihelmintic action. By Insilco studies we found the interaction of flavanoids with antihelmentic proteins. Key Word: Helminthes, ceasalpeniapulcherima, flavonoids, extractioin, in silico study.

Ecofriendly Green Synthesis and Characterisation of Silver Nanoparticles of Hyoscyamus Niger Seeds and Evaluation of its Anthelmintic Activity

Introduction: Nanotechnology is the design, characterisation and production of molecules or structures at nanometer scale. Metallic nanoparticles have been known for their therapeutic applications for thousands of years. Hyoscyamus niger is a traditional plant used as analgesic, sedative, anthelmintic, antitumour and febrifuge. Objective: An eco-friendly approach was made for the synthesis of silver nanoparticles using aqueos seed extract of Hyoscyamus niger (H.n-AgNPs) and checked for possible anthelmintic activity. Method: Preparation of silver nanoparticles in 1 mM aqueous silver nitrate from Hyoscyamus niger seed. The color shift from yellow to brownish red provided visible evidence of nanoparticle production. UV visible, Fourier transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM), and X-ray diffraction (XRD) examinations all validated it further. We tested the in vitro anthelmintic activity of various doses of H.n-AgNPs on Indian earthworms.. Results: The size of the H.n-AgNPs synthesized ranged between 8nm to183 nm. Synthesized NPs showed potential anthelmintic activity at 100µg/mlwith paralysis time and death time of 15 min and 34 min respectively where Albendazole was used as standard drug. The phytoconstituents like tannins and phenolic compounds may be responsible for precipitation of proteins or can attach with free proteins in gastrointestinal tract or glycoprotein on parasite cuticle and cause cell death. Conclusion: The H.n-AgNPs synthesized have potential anthelmintic activity which was concentration dependent. Further research will be done to design a new anthelmintic drug using H.n-AgNPs.

Synthesis, Computational Study of β-lactam Derivatives Bearing Quinazoline Schiff Bases as Anthelmintics

Background: Infections caused by parasites continue to pose a risk to both human and animal health. The most popular drug classes for treating these infections include thiabendazole, imidazothiazoles, levamisole, and avermectins (obtained from the fermentation products of Streptomyces avermitilis). The majority of bacteria that we come across in any form of infection are successfully combatted by β- lactam medicines. Similar to the majority of treatments, problems with treatment resistance to these drugs have evolved over time, necessitating the development of new anthelmintic medications. This study aims to investigate a series of quinazoline-bearing β-lactam rings as potent anthelmintic agents. Methods: The new series of quinazoline bearing β-lactam was synthesized via reaction of 5-bromo anthranilic acid with acetic anhydride to produce 6-bromo-2-methyl-4H-benzo (1,3) oxazin-4-one which was then converted into 3-amino-6-bromo-2-methylquinazolin-4(3H)-one by the reaction of hydrazine hydrate in the presence of anhydrous pyridine. The resultant intermediate then goes through a Schiff reaction with various aromatic aldehydes, followed by reflux with triethylamine and chloroacetyl chloride. Results: Structural assignments of these compounds have been made by elemental analysis, FTIR,1HNMR and Mass spectral data and the purity of the compounds was determined by TLC. Molecular docking studies showed the effective binding of synthesized derivatives with tubulin in comparison to the orientation of standard drugs. The anthelmintic activities of all the synthesized compounds were evaluated separately for their possible using common Indian earthworm Pheritima posthuma. Conclusion: A total of ten derivatives of quinazoline bearing β-lactam ring were designed, synthesized and evaluated against the Indian earthworm Pheritima posthuma. Out of ten derivatives, SQD2 and SQD6, showed promising anthelmintic activity when compared with standard drugs albendazole and piperazine citrate.

Exploration of anthelmintic potency of Pisonia alba

Helminthiasis or worm infestation is one of the most prevalent disease and one of the most serious problems in the world. Pisonia alba (Ceodeus Grandis) plant was selected for exploration of anthelmintic activity because Nyctaginaceae family plants such as Boerhavia diffusa, Bougainvillea glabra, and Mirabilis jalapa L., already tested for the anthelmintic activity and they have potent anthelmintic activity. The Phytochemical test performed in this ethanolic extract of Pisonia alba. This study indicated that the crude ethanol extract produces anthelmintic activity against the Indian earthworm Pheretima Posthuma. Ethanolic extract of Pisonia alba [20, 40, 60 mg/ml] compared with standard drug (Albendazole). The extract showed significant potency when compared with standard drug Albendazole. The plant explored significant anthelmintic activity at 60 mg/ml concentration in time taken for paralyse /death of the earthworms The current investigation indicates the leaves of Pisonia alba have potent anthelmintic activity the further study is needed to explore its full potential.

Why Natural Farming Technology be Adopted and Researches Intensified?

Rising environmental Pollution, decreasing soil fertility, augmenting ground water contamination with NO3, As, Pb, Cd, etc deterioratingvegetables, fruits, edible grains and milk quality due to poisonous chemicals and thereby increasing health hazards are great threatat global level. The chemical laden agriculture is one of the pivotal causes for such consequences and “Punjab cancer train” in Indiacarrying hundreds of cancer patients daily from Bhatinda (Punjab) to Bikaner (Rajasthan) for treatments, are well known. Natural farmingtechnology trained farmers from eleven states viz., Gujarat, Maharashtra, Uttar Pradesh, Himachal Pradesh, Madhya Pradesh, Uttarakhand,Meghalaya, Chhattisgarh, Kerala, Karnataka and Andhra Pradesh of India are getting spectacular higher yield and good quality pollutantfree crop harvest and edible products. This technology is comprised of treating seeds with Beejamrit and Ghanajeewamrit to improveseed germination and improve soil health and crop growth. Spraying of Neemastra, or Agniastra or Bramhastra are done to controlinsect, pests and plant diseases without polluting environment. Natural farming is broadly synonym to Vedic Agriculture, cosmic farmingand Homa therapy using Agnihotra ash. Promotion of microorganism, Indian earthworms, Indian breed cows and water conservationthrough crop residues or intercropping practices are the key epithets of natural farming. This technology has been found to be costeffective and pollution free, also called as ‘Zero Budget Agriculture’ since all the inputs are natural and domestically produced. The prosand cons of this technology with a view to make a sensible decision for its adoption at local, state, national and international level arediscussed in this paper.In case of some technological impetigo in attaining present level of crop productivity on large scale, the challenges before agriculturescientists are to ameliorate natural farming technology and maintain health friendly environment, harvest higher crop yield and assurefood security to burgeoning population.

Anthelmintic Activities of Methanol Extracts of Five Different Geophyte Plant Species

The purpose of this study was to look into the in vitro anthelmintic activities of methanol extracts of Hyacinthella lineata (Steud. ex Schult. & Schult.f.) Chouard, Ornithogalum umbellatum L., Allium reuterianum Boiss., Cyclamen coum Mill., and Sternbergia clusiana (Ker Gawl.) Ker Gawl. ex Spreng.. The paralysis and death times of the Indian earthworm, Cosmocerca ornata, were used to assess anthelmintic activity. Three different concentrations, (5,10 and 20 mg mL-1) of methanol extracts were used to determine the time required to paralyze and to induce death in the earthworms. Albendazole (15 mg mL-1) was used as reference standard drug. Plant extracts at higher concentrations (20 mg mL-1) produced faster paralytic effects and a shorter time to death. The results revealed that S. clusiana killed all of the test worms within 20 ± 0.6 min of exposure and was 100% effective. Among all five plants extracts tested, S. clusiana was found to be highly effective and significant against C. ornata. The findings will aid in understanding the significance and application of these medicinal plant species in the food industry, traditional medicine, and pharmaceutical applications. This anthelmintic study of the plant extracts revealed that traditional medicine may be as effective as modern medicine in combating pathogenic micro-organisms.

Exploring Schleichera oleasa crude bark extract for its anti-helminthic potential

Schleichera oleosa (Lour.) Oken or Kusum, belonging to family Sapindaceae is an important medicinal plant reported to possess various pharmacological activities viz. analgesic, antibiotic, anti-dysentery, anti-helminthic, antioxidant and anticancer activities. Scientific studies reporting antihelminthic properties of bark extract of this plant are still lacking, therefore, the present work has been designed to investigate the ethanolic and ethyl acetate extracts of bark for its antihelminthic potential. Antihelminthic evaluation was done using Indian earthworms, Pheretima posthuma by determining time of paralysis and death. Phytochemical screening of the extracts revealed the presence of flavonoids, tannins and phenolic acids. The anti-helminthic activity of both extracts (0.1%) was found better than the standard drug Albendazole. Since, both extracts showed promising results, they can be further helpful in designing herbal formulations for commercial use.dry-powder inhaler (DPI) carries medication to lungs as a dry powder, useful against respiratory diseases.

Comparitive Invitro Antihelmintic Activity of Individual and Combined Leaf Extracts (Syzygium cumini and Achras zapota) Against Pheritima Posthuma

Syzygium cumini and Achras zapota leaves were easily available and commonly used in our day –to-day life. The present study aimed at the in-vitro comparative study of anthelmintic activity of aqueous extracts of leaves of Syzygium cumini, Achras zapota and mixed. The fruits were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous extract of Syzygium cumini, Achras zapota and mixed showed anthelmintic activity but mixed extract was more efficient anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P< 0.0001).

Invitro Antihelmintic Activity of Aqueous Seed Extract of Syzygium cumini against Indian Earthworms

Syzygium cumini (L.) Skeels, commonly known as Jamun, is a widely distributed forest tree in India and other tropical and sub tropical regions of the world. The tree has a great economic importance since most of the parts like the bark, leaves, seed and fruits are used as an alternative medicine to treat various diseases. The present study aimed at the in-vitro anthelmintic activity of aqueous fruit seed extract of Syzygium cumini. The fruit seeds were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200,300,400mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous fruit seed extract of Syzygium cumini showed anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P< 0.001).

Invitro Antihelmintic Activity of Individual and Combined Leaf Extracts (Punica granatum and Psidium guajava) Against Pheritima Posthuma

Punica granatum and Psidium guajava leaves were easily available and commonly used in our day –to-day life. The present study aimed at the in-vitro comparative study of anthelmintic activity of aqueous extracts of leaves of Punica granatum, Psidium guajava and mixed. The fruits were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous extract of Punica granatum, Psidium guajava and mixed showed anthelmintic activity but mixed extract was more efficient anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P< 0.001).

Anthelmintic activity of (Mugwort) extracted with ethanol and rice gruel solvents

This laboratory study was carried out to explore the anthelmintic properties of (Mugwort) extracts aligned with Indian earthworms Alcoholic extract and Rice Gruel extract of leaves were used as test. Albendazole was incorporated as standard drug and normal saline as control. Observations were made for the time taken to paralyze and death of the earthworm. Three concentrations 30, 60, 100mg/ml of every extract and standard drug at the concentration of 30mg/ml were studied. The outcome of study indicated that rice gruel extract of exhibited anthelmintic activity significantly higher than standard and also in a dose dependent manner. For the ethanolic extract group the time of paralysis and death time was 3.38 minute and 13.82 minute, while rice gruel extract group showed shortest time of paralysis (P) at 2.12 minute and death (D) at 7.45 minute of earthworms at higher concentration 100mg/ml as compared to standard Albendazole (30mg/ml) paralysis 2.27 minute and death at 6.44 minute respectively. Rice gruel extract showed more pronounced effect than ethanolic extract. Whereas the control group worms were observed for full day and night and there was no paralysis or death was found during that period.

Assessment of Anthelmintic Activity and in silico Study of Phytoconstituents in Decaschistia crotonifolia Wight & Arn. Root Extract

Published on:June 2022Journal of Young Pharmacists, 2022; 14(2):169-173Original Article | doi:10.5530/jyp.2022.14.32 Authors: Raveesha Peeriga*, Keerthi Priyanka Adarapu, Kavya Sri Sanivarapu, Jyothsna Kanumuri, Rikith Swamy Akunuri, Lakshmana Rao Atmakuri Department of Pharmacognosy, V. V. Institute of Pharmaceutical Sciences, Gudlavalleru, Andhra Pradesh, INDIA. Abstract: Background: Worm infections in developing countries were reported high. Phytoconstituents have been a vital role for the treatment of many ailments. The current study was aimed assess for anthelmintic activity of different root extracts of Decaschistia crotonifolia belongs to the family Ebanaceae against Pheretima posthuma. Further Insilico study was carried out for phytocompounds present in Dechaschistia. Methods: The chloroform, ethylacetate and ethanol extract of Decaschistia crotonifolia were considered for the study of anthelmintic property on earthworms at concentrations 20 mg/ml, 40 mg/ml and 60 mg/ml. During this study, the parameters paralysis time and Death Time of adult Indian earthworms was observed. As a standard and control Albendazole 10 mg/ml and 2% Tween 80 in distilled water were taken respectively. Results: The study resulted that ethanolic extract was significant when compared with the Albendazole 10 mg/ml. Docking studies revealed all phytocompounds in Dechaschistia shown binding affinity, however comparatively scopoletin and stigmasterol had shown a good binding affinitiy about -7.7 Kcal/mol and -7.6 Kcal/mol compared to standard drug Albendazole which was shown about -8.7 Kcal/mol. Conclusion: The study revealed that the ethanol extract of Decaschistia crotonifolia at a concentration of 60mg/ml exhibited a stronger anthelmintic property compared to Albendazole 10mg/ml. A dose dependent anthelmintic activity is exerted by all the extracts in an ascending manner Chloroform<Ethyl acetate<Ethanol. These observations were made evidenced by docking studies of phytocompounds in Dechaschistia as the phytocompounds were shown excellent docking score when compared with standard Albendazole. Key words: Decaschistia crotonifolia Wight and Arn., Ebanaceae, Pheretima and Anthelmintic, Docking, Lipinski rule.

A comparative account of coelomocyte of earthworm ecotypes with reference to its morphology, morphometry, density, phagocytosis, autofluorescence, and oxidative status.

Earthworms inhabit different strata of moist soil. Epigeic and endogeic earthworms prefer superficial and inner stratum of soil respectively, whereas, semiaquatic species are distributed around hydrated soil near ponds and lakes. Coelomocytes, the chief immunoeffector cells of coelomic origin, perform diverse physiological functions like phagocytosis, maintenance of cellular homeostasis, and acid-base balance of coelomic fluid, graft rejection, elicitation of cytotoxic, and oxidative responses under the challenges of pathogens and toxins. The present study aims to analyze selected morphological and functional parameters in three differentially adapted Indian earthworms of nonsimilar habitats. Coelomocytes of Glyphidrilus tuberosus (Stephenson, 1916) (semiaquatic), Perionyx excavatus (Perrier, 1872) (epigeic), and Eutyphoeus orientalis (Beddard, 1883) (endogeic) were isolated for morphological and morphometric analyses and subjected to determination of phagocytic, oxidative, and cytotoxic responses. Activities of phenoloxidase, pro, and antioxidant enzymes, and autofluorescence were determined in the extruded coelomocytes of earthworms of three contrasting habitats. The differential result may be correlated with species-specific responses and variation in habitat preference and related adaptation.

Anthelmintic Activity of liquid Self Emulsifying Drug Delivery System (SEDDS) of Praziquntel

The present study aims at developing a new adaptable method for evaluation of anthelmintic activity. Liquid self emulsifying drug delivery system was prepared by using design expert method. The anthelmintic activity of emulsion was evaluation by using Indian earthworms at doses 2.5,5,10,15,20 mg/ml. The Praziquantel was used as standard drug (10 mg/ml).The paralysis and death time of earthworms after administering doses were determined. The result of anthelmintic activity of earthworms showed that the earthworms had taken less time for paralysis and less time for death. It can be concluded that earthworms can be used successfully for the anthelmintic activity study as it is easy, prominent, an adaptable to laboratory conditions. Evaluation of anthelmintic activity of any drug when carried out in laboratory conditions by using the isolated worms from nature cannot be adaptable with artificial laboratory conditions. The present anthelmintic activity study reveals a new methodology with earth worms cultured in laboratory conditions. We studied the anthelmintic activities of an Praziquantel drug on earthworms. This result showed that the earthworms had taken less time for paralysis and death. This novel dosage form might be a promising dosage form in the prevention of worm infections for pediatric patients.

Stomach-affecting intestinal parasites as a precursor model of Pheretima posthuma treated with anthelmintic drug from Dodonaea viscosa Linn.

Abstract The preponderance of helminth contagions is constrained to tropical regions and can cause massive vulnerability to malnutrition, anemia, pneumonia, and eosinophilia. Many human parasitic diseases cause severe illness in endemic populations. The helminths transmitting through the gastro-intestinal tract may develop resistance to anthelmintic drugs. The phytotherapy, anthelmintic, and antimicrobial efficacy of Dodonaea viscosa leaf solvent extracts were examined. Phytochemical screening was carried out by ultraviolet (UV) and Fourier transform infrared spectroscopy (FTIR). The anthelmintic activity was performed against a South Indian earth worm as a model by measuring the paralytic time. The antibacterial activity was performed against Bacillus subtilis, Escherichia coli, Klebsiella pneumoniae, and Staphylococcus aureus, which stimulate the stomach helminths. Phytochemical screening revealed the presence of chemical compounds from the UV spectrum, and alkenes and aromatic compounds were confirmed by FTIR. Higher concentrations of the D. viscosa extract showed a rapid paralytic effect with a rapid death rate and histopathology. The zone-of-inhibition study indicated the potent antibacterial activity of the ethanolic and methanolic extracts of D. viscosa against different bacterial species. The current research revealed that D. viscosa has significant anthelmintic and antibacterial activities and it can be used for further elucidation and characterization.

Comparitive Invitro Antihelmintic Activity of Electeria cardamom and Amomum subulatum against Pheritima Posthuma

Elettaria cardamomum and Amomum subulatum fruits were easily available and commonly used in our day –to-day life. The present study aimed at the in-vitro comparative study of anthelmintic activity of aqueous extracts of fruits of Elettaria cardamomum, Amomum subulatum and mixed. The fruits were extracted separately with distilled water by maceration method .The various concentrations of the extract (50,100, 200mg/ml) respectively were screened for their anthelmintic activity using Pheritima posthuma. The activity was comparable with the standard drug albendazole. When the concentrations of the extract are increased, a gradual increase in anthelmintic activity is observed. The study involved the determination of time of paralysis (P) and time of death (D) of the worms. Aqueous extract of Elettaria cardamomum, Amomum subulatum and mixed showed anthelmintic activity but mixed extract was more efficient anthelmintic activity against Indian earthworms. The data were found statistically significant by using one way ANOVA (P&lt; 0.001). Further studies are suggested to isolate the active principles responsible for the activity.

Synthesis, Antibacterial and Anthelmintic Activity of Novel 3-(3-Pyridyl)-oxazolidinone-5-methyl Ester Derivatives.

In this study, a series of 3-(3-pyridyl)-oxazolidone-5-methyl ester derivatives was synthesized and characterized by 1H NMR, 13C NMR, and LC-MS. The conducted screening antibacterial studies of the new 3-(3-pyridyl)-oxazolidone-5-methyl ester derivatives established that the methyl sulfonic acid esters have broad activity spectrum towards Staphylococcus aureus, Streptococcus pneumoniae, Bacillus subtilis and Staphylococcus epidermidis. Among them, compound 12e has the most potent activity, with an MIC of 16 μg/mL against B.subtilis, and could reduce the instantaneous growth rate of bacteria. Furthermore, molecular docking studies were also simulated for compound 12e to predict the specific binding mode of this compound. In addition, anthelmintic activity of these compounds was also evaluated against adult Indian earthworms (Pheretima posthuman). The results showed that compound 11b had the best effect. These results above can provide experimental reference for the development of novel antibacterial and anthelmintic drugs.

PHYTOCHEMICAL AND ANTHELMINTIC ACTIVITY INVESTIGATIONS EXTRACT OF Mimosa pudica Linn. ROOT EXTRACT

Mimosa pudica L. is commonly called sensitive plant it’s belongs to the family of Mimosaceae. The whole plant was highly medicinal properties and important used in several preparations of ayurvedic medicine. It has been reported that the phytochemical compounds revealed the presence of most highly in toxic alkaloids as mimosine and orientin, isoorientin, D-pinitol, norepinephrine mucilage, tannins, non-protein amino acid, tannins, flavonoids, C glycosides, steroids, terpenoids, fatty acids, saponins, coumarin, are also present. The anthelmintic activity of root was studied on adult Indian earthworm. Pheretima posthuma in 3-5 cm in length and 0.1-2 cm in width were used due to its anatomical and physiological resemblance with the intestinal roundworm parasite of human beings. The anthelmintic assay was carried out as per the method of Nargud with minor modification. 100 mg/ml, 200 mg/ml and 500 mg/ml dilutions of petroleum ether, ethanol and aqueous extract were prepared. Normal saline and albendazole served as control.