Independent Regulation Research Articles

Metamaterial Adaptive Frequency Switch Rectifier Circuit for Wireless Power Transfer System

In this paper, a novel metamaterial adaptive frequency switch (MAFS) rectifier circuit is proposed to expand the operating frequency range of the rectifier circuits for wireless power transfer (WPT) systems. Due to the nonlinearity and complex input impedance of the rectifying circuit, the MAFS technology is introduced to improve the matching performance of the rectifier circuit working in different bands. We can use MAFS technology to achieve independent regulation of a single rectifier circuit at different bands, simplifying the rectifier's topology and complexity. A dual-band rectifier circuit and a broadband rectifier circuit are designed to verify the feasibility of the structure. The experimental results show that the designed dual-band rectifier circuit has high conversion efficiency (more than 50%) in two broad bands, 3.2-3.6 and 5.2-6.2 GHz, for 5G and Wi-Fi bands. In addition, the conversion efficiency of the designed broadband rectifier circuit from 4.7 to 6.2 GHz is higher than 50% at 13 dBm. The fractional bandwidth is expanded by 10% compared to the multiplexed rectifier circuit structure. Therefore, the proposed rectifier circuit structure can significantly broaden the operating frequency range and has a simple design, which can be widely used in a wireless power transfer system.

Methamphetamine-induced region-specific transcriptomic and epigenetic changes in the brain of male rats

Psychostimulant methamphetamine (METH) is neurotoxic to the brain and, therefore, its misuse leads to neurological and psychiatric disorders. The gene regulatory network (GRN) response to neurotoxic METH binge remains unclear in most brain regions. Here we examined the effects of binge METH on the GRN in the nucleus accumbens, dentate gyrus, Ammon’s horn, and subventricular zone in male rats. At 24 h after METH, ~16% of genes displayed altered expression and over a quarter of previously open chromatin regions - parts of the genome where genes are typically active - showed shifts in their accessibility. Intriguingly, most changes were unique to each area studied, and independent regulation between transcriptome and chromatin accessibility was observed. Unexpectedly, METH differentially impacted gene activity and chromatin accessibility within the dentate gyrus and Ammon’s horn. Around 70% of the affected chromatin-accessible regions in the rat brain have conserved DNA sequences in the human genome. These regions frequently act as enhancers, ramping up the activity of nearby genes, and contain mutations linked to various neurological conditions. By sketching out the gene regulatory networks associated with binge METH in specific brain regions, our study offers fresh insights into how METH can trigger profound, region-specific molecular shifts.

Single‐stage triple‐output Cuk power factor correction converter

SummaryTraditional multiple‐output AC‐DC power supply with power factor correction (PFC) function usually employs a two‐stage cascaded topology that is composed of prestage PFC converter and N independent poststage DC‐DC converters to achieve PFC and voltage or current regulation of N outputs. Hence, the conventional two‐stage cascaded multiple‐output AC‐DC power supply needs N + 1 magnetic components as power inductors or transformers and N + 1 controllers, which increases the cost and size of the multioutput converters. The conventional multiple‐string AC‐DC LED driving circuits usually employ a two‐stage topology to realize PFC and independent current regulation of each LED string. To address the issue of complexity, high cost, and large size of the two‐stage multistring LED driving circuit, a single‐stage triple‐output Cuk PFC converter is proposed by cascading boost converter and single‐inductor triple‐output buck converter with sharing main power switch in this paper. The topology operation principle, control strategy, design consideration, and operation characteristics of the proposed circuit are investigated in detail. With the switch sharing and inductor multiplexing technologies at the same time, the proposed converter utilizes only single‐stage converter to achieve PFC function, independent regulation of triple‐channel output current, and flicker‐free with low twice line frequency LED current ripple. The proposed converter is verified by a 50‐W experimental prototype of LED driver for triple string LEDs. The proposed converter only uses two power inductors and a set of controller to achieve greater than 0.945 PF, up to 90.5% efficiency, low LED current ripple, and independent current regulation for triple LED strings with different forward voltage, which may significantly reduce the cost and size of the traditional AC‐DC multistring LED driving circuit.

Time of day and genotype sensitivity adjust molecular responses to temperature stress in sorghum.

Sorghum is one of the four major C4 crops that are considered to be tolerant to environmental extremes. Sorghum shows distinct growth responses to temperature stress depending on the sensitivity of the genetic background. About half of the transcripts in sorghum exhibit diurnal rhythmic expressions emphasizing significant coordination with the environment. However, an understanding of how molecular dynamics contribute to genotype-specific stress responses in the context of the time of day is not known. We examined whether temperature stress and the time of day impact the gene expression dynamics in thermo-sensitive and thermo-tolerant sorghum genotypes. We found that time of day is highly influencing the temperature stress responses, which can be explained by the rhythmic expression of most thermo-responsive genes. This effect is more pronounced in thermo-tolerant genotypes, suggesting a stronger regulation of gene expression by the time of day and/or by the circadian clock. Genotypic differences were mostly observed on average gene expression levels, which may be responsible for contrasting sensitivities to temperature stress in tolerant versus susceptible sorghum varieties. We also identified groups of genes altered by temperature stress in a time-of-day and genotype-specific manner. These include transcriptional regulators and several members of the Ca2+ -binding EF-hand protein family. We hypothesize that expression variation of these genes between genotypes along with time-of-day independent regulation may contribute to genotype-specific fine-tuning of thermo-responsive pathways. These findings offer a new opportunity to selectively target specific genes in efforts to develop climate-resilient crops based on their time-of-day and genotype variation responses to temperature stress.

Fermentation of Ganoderma lucidum and Raphani Semen with a probiotic mixture attenuates cyclophosphamide-induced immunosuppression through microbiota-dependent or -independent regulation of intestinal mucosal barrier and immune responses

BackgroundProbiotic fermentation is a promising strategy for improving the nutritional and functional properties of traditional Chinese medicines (TCMs). Ganoderma lucidum and Raphani Semen are famous TCMs that have been shown to help alleviate immune system disorders. However, few studies have experimentally investigated the effects of probiotic-fermented G.lucidum and Raphani Semen on the immune system. PurposeWe established the in vitro fermentation of G. lucidum and Raphani Semen with a probiotic mixture (Bifidobacterium longum, Lactobacillus acidophilus, and l. fermentum) (GRFB), investigated its ameliorating effect against cyclophosphamide (CTX)-induced immunosuppression, and explored its possible mechanisms. MethodsFirst, the different components in GRFB were identified by high-performance liquid chromatography. Second, its immune-stimulatory activities were evaluated in CTX-treated mice. Lastly, its possible in vitro and in vivo mechanisms were studied. ResultsProbiotic fermentation of G. lucidum and Raphani Semen altered some of its chemical constituents, potentially helping improve the ability of GRFB to alleviate immunosuppression. As expected, GRFB effectively ameliorated CTX-induced immunosuppression by increasing the number of splenic lymphocytes and regulating the secretion of serum and ileum cytokines. GRFB supplementation also effectively improved intestinal integrity in CTX-treated mice by upregulating tight junction proteins. It also protects against CTX-induced intestinal dysbiosis by increasing the abundance of beneficial bacteria and reducing the abundance of harmful bacteria. GRFB could directly promote intestinal immunity but not systemic immunity in vitro, suggesting a microbiota-dependent regulation of GRFB. Interestingly, cohousing CTX-induced immunosuppressed mice with GRFB-treated mice promoted their symptoms recovery. Enhanced CTX-induced immunosuppression by GRFB in vitro depended on the gut microbiota. Remarkably, a Kyoto Encyclopedia of Genes and Genomes analysis showed that the GRFB-reprogrammed microbiota was significantly enriched in DNA damage repair pathways, which contribute to repairing the intestinal mucosal barrier. ConclusionThis is the first study to suggest that compare with unfermented G. lucidum and Raphani Semen, GRFB can more effectively promote intestinal immunity and manipulate the gut microbiota to promote immunostimulatory activity and repair immunosuppression-induced intestinal barrier damage by biotransforming G.lucidum and Raphani Semen components.

Independent wavefront manipulation of broadband spin-decoupled metasurfaces based on equivalent circuit model

Various functional meta-devices have exhibited powerful manipulation of orbital angular momentum spectrums, but they are still faced with the issue of inherent spin-correlated characteristic and lack of effective method to design metasurface. In this work, a broadband spin-decoupled metasurface is presented to realize independent regulation of dual circularly polarized waves. Based on the transmission line theory, an equivalent circuit model of meta-atom is proposed to establish the relationship between reflection coefficients and structural parameters. By utilizing the model, a group of meta-atoms has been designed with a step phase difference of 45°, which can form a 3-bit coding metasurface. The proposed meta-atoms have a broad frequency band covering 11.56 − 18.17 GHz (44.5%). Moreover, the bifunctional reflection-type metasurfaces are constructed for producing the vortex waves along the +z axis, the off-axis pencil beams, and their synthetic beams. Based on the Fourier transform analysis, the mode purity and the electric field distribution of the generated beams are discussed in detail. The experimental measurements have good agreement with the simulation results, thus revealing the effectiveness of the proposed methodology to design metasurface.

When drought meets heat - a plant omics perspective.

Changes in weather patterns with emerging drought risks and rising global temperature are widespread and negatively affect crop growth and productivity. In nature, plants are simultaneously exposed to multiple biotic and abiotic stresses, but most studies focus on individual stress conditions. However, the simultaneous occurrence of different stresses impacts plant growth and development differently than a single stress. Plants sense the different stress combinations in the same or in different tissues, which could induce specific systemic signalling and acclimation responses; impacting different stress-responsive transcripts, protein abundance and modifications, and metabolites. This mini-review focuses on the combination of drought and heat, two abiotic stress conditions that often occur together. Recent omics studies indicate common or independent regulators involved in heat or drought stress responses. Here, we summarize the current research results, highlight gaps in our knowledge, and flag potential future focus areas.

Optineurin tunes outside-in signaling to regulate lysosome biogenesis and phagocytic clearance in the retina

Balancing the competing demands of phagolysosomal degradation and autophagy is a significant challenge for phagocytic tissues. Yet how this plasticity is accomplished in health and disease is poorly understood. In the retina, circadian phagocytosis and degradation of photoreceptor outer segments by the postmitotic retinal pigment epithelium (RPE) are essential for healthy vision. Disrupted autophagy due to mechanistic target of rapamycin (mTOR) overactivation in the RPE is associated with blinding macular degenerations; however, outer segment degradation is unaffected in these diseases, indicating that distinct mechanisms regulate these clearance mechanisms. Here, using advanced imaging and mouse models, we identify optineurin as a key regulator that tunes phagocytosis and lysosomal capacity to meet circadian demands and helps prioritize outer segment clearance by the RPE in macular degenerations. High-resolution live-cell imaging implicates optineurin in scissioning outer segment tips prior to engulfment, analogous to microglial trogocytosis of neuronal processes. Optineurin is essential for recruiting light chain 3 (LC3), which anchors outer segment phagosomes to microtubules and facilitates phagosome maturation and fusion with lysosomes. This dynamically activates transcription factor EB (TFEB) to induce lysosome biogenesis in an mTOR-independent, transient receptor potential-mucolipin 1 (TRPML1)-dependent manner. RNA-seq analyses show that expression of TFEB target genes temporally tracks with optineurin recruitment and that lysosomal and autophagy genes are controlled by distinct transcriptional programs in the RPE. The unconventional plasma membrane-to-nucleus signaling mediated by optineurin ensures outer segment degradation under conditions of impaired autophagy in macular degeneration models. Independent regulation of these criticalclearance mechanisms would help safeguard the metabolic fitness of the RPE throughout the organismal lifespan.

Osh-dependent and -independent Regulation of PI4P Levels During Polarized Growth of Saccharomyces cerevisiae.

Polarized secretion facilitates polarized cell growth. For a secretory vesicle to dock at the plasma membrane, it must mature with a progressive association or dissociation of molecules that are, respectively, necessary for or inhibitory to vesicle docking, including an exchange of Rab GTPases. In current models, oxysterol-binding protein homologue 4 (Osh4p) establishes a phosphatidylinositol 4-phosphate (PI4P) gradient along the secretory trafficking pathway such that vesicles have higher PI4P levels after budding from the trans-Golgi relative to when vesicles arrive at the plasma membrane. In this study, using the lipid-binding domain P4M and live-cell imaging, we show that secretory vesicle-associated PI4P levels remain constant when vesicles traffic from the trans-Golgi to the plasma membrane. We also show that deletion of OSH4 does not alter vesicle-associated PI4P levels, though loss of any individual member of the OSH family or complete loss of OSH family function alters the intracellular distribution of PI4P. We propose a model in which the Rab GTPases Ypt32p and Sec4p remain associated with a secretory vesicle during trafficking, independent of PI4P levels and Osh4p. Together these data indicate the necessity of experiments revealing the location and timing of events required for vesicle maturation.

Epistatic Relationship between MGV1 and TRI6 in the Regulation of Biosynthetic Gene Clusters in Fusarium graminearum.

Genetic studies have shown that the MAP kinase MGV1 and the transcriptional regulator TRI6 regulate many of the same biosynthetic gene clusters (BGCs) in Fusarium graminearum. This study sought to investigate the relationship between MGV1 and TRI6 in the regulatory hierarchy. Transgenic F. graminearum strains constitutively expressing MGV1 and TRI6 were generated to address both independent and epistatic regulation of BGCs by MGV1 and TRI6. We performed a comparative transcriptome analysis between axenic cultures grown in nutrient-rich and secondary metabolite-inducing conditions. The results indicated that BGCs regulated independently by Mgv1 included genes of BGC52, whereas genes uniquely regulated by TRI6 included the gene cluster (BGC49) that produces gramillin. To understand the epistatic relationship between MGV1 and TRI6, CRISPR/Cas9 was used to insert a constitutive promoter to drive TRI6 expression in the Δmgv1 strain. The results indicate that BGCs that produce deoxynivalenol and fusaoctaxin are co-regulated, with TRI6 being partially regulated by MGV1. Overall, the findings from this study indicate that MGV1 provides an articulation point to differentially regulate various BGCs. Moreover, TRI6, embedded in one of the BGCs provides specificity to regulate the expression of the genes in the BGC.

β-Endorphin (an endogenous opioid) inhibits inflammation, oxidative stress and apoptosis via Nrf-2 in asthmatic murine model

Asthma, a chronic respiratory disease is characterized by airway inflammation, remodelling, airflow limitation and hyperresponsiveness. At present, it is considered as an umbrella diagnosis consisting several variable clinical presentations (phenotypes) and distinct pathophysiological mechanisms (endotypes). Recent evidence suggests that oxidative stress participates in airway inflammation and remodelling in chronic asthma. Opioids resembled by group of regulatory peptides have proven to act as an immunomodulator. β-Endorphin a natural and potent endogenous morphine produced in the anterior pituitary gland play role in pain modulation. Therapeutic strategy of many opioids including β-Endorphin as an anti‑inflammatory and antioxidative agent has not been yet explored despite its promising analgesic effects. This is the first study to reveal the role of β-Endorphin in regulating airway inflammation, cellular apoptosis, and oxidative stress via Nrf-2 in an experimental asthmatic model. Asthma was generated in balb/c mice by sensitizing with 1% Toulene Diisocyanate on day 0, 7, 14 and 21 and challenging with 2.5% Toulene Diisocyanate from day 22 to 51 (on every alternate day) through intranasal route. β-Endorphin (5 µg/kg) was administered through the nasal route 1 h prior to sensitization and challenge. The effect of β-Endorphin on pulmonary inflammation and redox status along with parameters of oxidative stress were evaluated. We found that pre-treatment of β-Endorphin significantly reduced inflammatory infiltration in lung tissue and cell counts in bronchoalveolar lavage fluid. Also, pre-treatment of β-Endorphin reduced reactive oxygen species, Myeloperoxidase, Nitric Oxide, Protein and protein carbonylation, Glutathione Reductase, Malondialdehyde, IFN-γ, and TNF-α. Reversely, β-Endorphin significantly increased Superoxide dismutase, Catalase, glutathione, Glutathione-S-Transferase, and activation of NF-E2-related factor 2 (Nrf-2) via Kelch-like ECH-associated protein 1 (Keap1), independent pathway in the lung restoring architectural alveolar and bronchial changes. The present findings reveal the therapeutic potency of β-END in regulating asthma by Keap-1 independent regulation of Nrf-2 activity. The present findings reveal the therapeutic potency of β-Endorphin in regulating asthma.

Sensible Factors that Need Strategic Change to Improve the Medical Drug Availability across Healthcare Institutions in Sri Lanka

Abstract : Access to medicines remains a major obstacle for people across the world to maintain their health at a desired level. Ensuring an adequate supply of safe and effective drugs of acceptable quality is an integral part of the health policy of Sri Lanka. The medical supplies division is the sole organization responsible for providing all pharmaceuticals, surgical items, laboratory items, radioactive items, and printed materials for government sector healthcare institutions. The establishment of an independent drug regulation authority, the Declaration of a national essential drug list, the establishment of a separate drug procurement entity, Pooled procurement, digitalization of drug management, and expansion of drug storage capacity are key strategies that have been implemented by the Ministry of Health to minimize the drug shortages across the healthcare institutions of Sri Lanka. Despite the implemented strategies drug shortage continue to evolve from time to time as a public outcry in the media. This case study describes the critical issues that need to look at more strategically to improve drug availability.

Contracts in Financial Law: Concept and Features

The paper examines the need to highlight at the legislative level an independent institution of the contract in financial law. Purpose and objectives of the study: to determine how relevant for the public branches of law at the present stage of development of domestic legislation is the formation of an independent institution of contractual relations, taking into account the convergence of public and private principles. Based on a formal legal analysis of the available scientific papers on the topic of the study, a conclusion is made about the importance of introducing the legal term “financial public contract” into legal circulation. Through comparison, the author comes to the conclusion that there is a dual understanding of the contract in financial law: on the one hand, as a formalized source of law, on the other, as an individual law enforcement agreement. At the same time, the latest interpretation of the financial public contract meets modern demands in the field of achieving government objectives by attracting the private sector both as a performer and an investor. The author’s understanding of the financial public contract is formulated. Improving the legal framework is possible only on the basis of sufficient doctrinal research on this issue, taking into account the opinion of law enforcers. It is necessary to continue to form a scientific and applied justification for the independent legal regulation of contracts in the public branches of Russian law, including financial law.

Гражданско-правовой режим географического указания в системе средств индивидуализации товаров, работ и услуг

The author correlates a set of features defining the legal regime of such a novel of civil legislation in the system of means of individualization of goods as a geographical indication with attributes characteristic of the designation of the place of origin of goods, which has been settled in the domestic legal order for a long time. An attempt is also made to differentiate the two mentioned means of individualization in order to identify the validity of their independent regulation.

Constitutively active autophagy in macrophages dampens inflammation through metabolic and post-transcriptional regulation of cytokine production

Autophagy is an essential cellular process that is deeply integrated with innate immune signaling; however, studies that examine the impact of autophagic modulation in the context of inflammatory conditions are lacking. Here, using mice with a constitutively active variant of the autophagy gene Beclin1, we show that increased autophagy dampens cytokine production during a model of macrophage activation syndrome and in adherent-invasive Escherichia coli (AIEC) infection. Moreover, loss of functional autophagy through conditional deletion of Beclin1 in myeloid cells significantly enhances innate immunity in these contexts. We further analyzed primary macrophages from these animals with a combination of transcriptomics and proteomics to identify mechanistic targets downstream of autophagy. Our study reveals glutamine/glutathione metabolism and the RNF128/TBK1 axis as independent regulators of inflammation. Altogether, our work highlights increased autophagic flux as a potential approach to reduce inflammation and defines independent mechanistic cascades involved in this control.

Broad Stopband, Low-Loss, and Ultra-Compact Dual-Mode Bandpass Filter Based on HMSIRC

In this investigation, an ultra-compact dual-mode bandpass filter (BPF) with a wide stopband response is realized by using a half-mode substrate-integrated rectangular cavity (HMSIRC). The HMSIRC resonator is designed with a cavity that is rectangular in shape and has metallic vias along three of the sides. The fourth side is open-ended and contains microstrip feed lines. For the purpose of constructing a magnetic wall, a rectangular slot is cut into each of the HMSIRC’s three edges. In order to produce an electrical wall that may generate a variety of resonances, the side with the open edges is provided with a single metallic via in the center. After that, a second-order BPF is generated by loading a transverse slot in the middle of the BPF, which enables independent frequency regulation of the mode frequencies. The eigen-mode analysis; field distributions; coupling matrix; and full-wave simulation of the proposed HMSIRC filter topology are used to develop the working principle of the filter. A second-order BPF is realized, constructed, and experimentally validated in order to provide evidence that the theory is correct. The BPF prototype achieves satisfactory performance thanks to its compact footprint of 0.028 λg2; its broad passband of 15.9%; its low insertion loss of 0.41 dB; and its wide stopband of 4.36 f0 with a rejection level greater than 20 dB. Both the measured and EM-simulated responses of the BPF are very consistent with one another.

Threading dislocation and lattice stress modulation of Si based GaN material with AlPN nucleation layer

An innovative AlPN nucleation layer is proposed for the growth of high quality GaN on Si. AlPN nucleation layer is prepared on Si substrate by metal organic chemical vapor deposition to explore the stress and dislocation adjustment mechanism. It is found that there are stress-related antisite defects in the P-doped lattice. In addition, the incorporation of P makes the dislocation densities of screw dislocations and edge dislocations have different trends, which can realize the independent control of the different types of dislocation densities. A hexagonal-cubic-phase mixed structure model is proposed to analyze and explain the variation trends of dislocation densities and stress. This work not only puts forward a new type of nitride material, which provides an idea for the growth of high-quality GaN on Si. On the other hand, it is of great significance to perfect the independent regulation mechanism of dislocations.

An independent regulator of global release pathways in astrocytes generates a subtype of extracellular vesicles required for postsynaptic function.

Extracellular vesicles (EVs) are heterogeneous in size, composition, and function. We show that the six-transmembrane protein glycerophosphodiester phosphodiesterase 3 (GDE3) regulates actin remodeling, a global EV biogenic pathway, to release an EV subtype with distinct functions. GDE3 is necessary and sufficient for releasing EVs containing annexin A1 and GDE3 from the plasma membrane via Wiskott-Aldrich syndrome protein family member 3 (WAVE3), a major regulator of actin dynamics. GDE3 is expressed in astrocytes but not neurons, yet mice lacking GDE3 [Gde3 knockout (KO)] have decreased miniature excitatory postsynaptic current (mEPSC) amplitudes in hippocampal CA1 neurons. EVs from cultured wild-type astrocytes restore mEPSC amplitudes in Gde3 KOs, while EVs from Gde3 KO astrocytes or astrocytes inhibited for WAVE3 actin branching activity do not. Thus, GDE3-WAVE3 is a nonredundant astrocytic pathway that remodels actin to release a functionally distinct EV subtype, supporting the concept that independent regulation of global EV release pathways differentially regulates EV signaling within the cellular EV landscape.

Independent regulation of ionomer distribution in catalyst layer for proton exchange membrane fuel cell

The ionomer distribution in the catalyst layer (CL) of proton exchange membrane fuel cell (PEMFC) has become a vital research topic, especially under the low Pt loading condition. In the drying process, the ionomers adsorbed on Pt/C particles in the catalyst ink (called adsorbed ionomers) evolve into a thin ionomer coating on catalyst particles, while free ionomers in the catalyst ink randomly deposit to form ionomer bridges (called deposited ionomers). Many studies have reported the structure and regulation of adsorbed ionomers, but little is known about deposited ionomers which are also important for the formation of CL structure. In this work, a two-pronged CL preparation method is innovatively proposed to achieve independent regulation of the deposited ionomer formed by free ionomers in the catalyst ink, and the effects of different deposited ionomer structures on CL microstructure and fuel cell performance are investigated. Furthermore, the two-pronged method is compared with the conventional method to discuss its feasibility in optimizing the CL structure. The results indicate that the characteristics of free ionomer in the catalyst ink will affect the ionomer distribution and pore structure in the CL by controlling the deposited ionomer, thus determining proton conduction and mass transport. The uniform deposited ionomer morphology and short-side-chain (SSC) ionomer structure make the ionomer distribution in the CL uniform, which promotes proton conduction but also results in high mass transport resistance. Compared with the conventional method, the proton conduction resistance of the electrode prepared by the two-pronged method is decreased by 54.7%, the thickness of ionomer film on catalyst particles surface is decreased by about 2 nm, and the power density of the single cell is increased by 40.4% at the current density of 2500 mA cm−2. These findings provide new insights into the independent regulation of each component in the catalyst ink and the structural optimization of CL.

The Effect of Countries' Independent Regulation on Cryptocurrency Markets

Cryptocurrencies have increasingly been traded against fiat currencies and as a result, governments globally have been trying to regulate these largely decentralized currencies. In this study, event study methodology has been used to evaluate the effect of regulatory announcements made by 25 countries. Based on cryptocurrency usage and returns of three major cryptocurrencies, namely Bitcoin, Ether, and XRP, this study finds that regulatory news results in significant abnormal returns for Bitcoin and Ether, but not for XRP. The authors find that irrespective of the type of news, abnormal returns are almost always negative. The countries have also been clustered based on their abnormal returns and it has been found that country characteristics such as income level, technological readiness and innovation potential affect the magnitude of abnormal returns. Thus, cryptocurrencies being global in essence, their regulatory oversight in countries do not exist in isolation, but they are also affected by the countries' development.