Increased Blood Glucose Levels Research Articles

Probiotic Foods Are Effective on Weight Loss, Biochemical Parameters, and Intestinal Microbiota in Wistar Albino Rats with Obese Microbiota.

The positive effects of various probiotic foods on weight control, intestinal microbiota, and biochemical markers have been proven by various studies. However, there is no study on such effects of tarhana and kefir + tarhana consumption, a type of Turkish food rich in Lactobacillus spp., Pediococcus pentosaceus, Pediococcus acidilactici, and Saccharomyces cerevisiae. This study aimed to determine the changes caused by regular consumption of kefir and/or tarhana for 6 months on weight gain, intestinal microbiota, and biochemical parameters in Wistar albino rats with obese microbiota. Therefore, thirty-five rats were fed with five different methods of oral gavage (n = 7 per group): Normal Diet Control (NDC), High Fat Diet Control (HFDC), 6 mL/kg Kefir + High Fat Diet (Kefir + HFD), 0.2 g/kg Tarhana + High Fat Diet (Tarhana + HFD), and 6 mL/kg Kefir + 0.2 g/kg Tarhana + High Fat Diet (Kefir + Tarhana + HFD). Normality tests were evaluated using the One-Sample Kolmogorov test and Histogram graph. Multiple group comparisons were performed using one-way ANOVA and Tukey's HSD post hoc test, and the statistical significances were indicated by different letters (p < 0.05). Comparisons by gender were performed using the independent samples t-test. Kefir consumption was more effective on decreasing weight gain. Obese microbiota significantly increased blood glucose level and decreased red blood cell (RBC), hematocrit (HCT), hemoglobin, mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), platelets (PLT), and white blood cells. RBC and HCT values in Kefir + HFD, PLT value in Tarhana + HFD, and mean corpuscular volume (MCV), MCH, and MCHC values in Kefir + Tarhana + HFD were higher than those of other groups (p < 0.05). Kefir + tarhana consumption significantly showed an increase in blood glucose. Kefir and/or tarhana induced the abundance of Lactobacillus and blocked the abundances of total coliform bacteria and Escherichia coli (p < 0.05). We demonstrated that kefir was effective in decreasing weight gain, and all dietary interventions induced positive alterations on biochemical findings and intestinal microbiota.

Strategies to Improve Insulin Delivery through Oral Route: A Review.

Diabetes mellitus is found to be among the most suffered and lethal diseases for mankind. Diabetes mellitus type-1 is caused by the demolition of pancreatic islets responsible for the secretion of insulin. Insulin is the peptide hormone (anabolic) that regulates the metabolism of carbohydrates, fats, and proteins. Upon the breakdown of the natural process of metabolism, the condition leads to hyperglycemia (increased blood glucose levels). Hyperglycemia demands outsourcing of insulin. The subcutaneous route was found to be the most stable route of insulin administration but faces patient compliance problems. Oral Insulin delivery systems are the patient-centered and innovative novel drug delivery system, eliminating the pain caused by the subcutaneous route of administration. Insulin comes in contact across various barriers in the gastrointestinal tract, which has been discussed in detail in this review. The review describes about the different bioengineered formulations, including microcarriers, nanocarriers, Self-Microemulsifying Drug Delivery Systems (SMEDDs), Self-Nanoemulsifying drug delivery systems (SNEDDs), polymeric micelles, cochleates, etc. Surface modification of the carriers is also possible by developing ligand anchored bioconjugates. A study on evaluation has shown that the carrier systems facilitate drug encapsulation without tampering the properties of insulin. Carrier-mediated transport by the use of natural, semi-synthetic, and synthetic polymers have shown efficient results in drug delivery by protecting insulin from harmful environment. This makes the formulation readily acceptable for a variety of populations. The present review focuses on the properties, barriers present in the GI tract, overcome the barriers, strategies to formulate oral insulin formulation by enhancing the stability and bioavailability of insulin.

Cadmium perturbed metabolomic signature in pancreatic beta cells correlates with disturbed metabolite profile in human urine.

Cd exposure has been demonstrated to induce a variety of metabolic disorders accompanied with imbalance of glucose and lipid homeostasis. The metabolic toxicity of Cd exposure at metabolome-wide level remains elusive. In our study, we demonstrated that Cd exposure via drinking water increased blood glucose levels, decreased serum insulin levels, led to glucose intolerance and suppressed insulin expression in the pancreas of C57/6J mice. Cd exposure significantly inhibited cell viability and suppressed insulin secretion in MIN6 cells in vitro. Since pancreatic β-cells are the only source of insulin production in the body and play a pivotal role in modulating glucose and lipid metabolisms, we further delineated the metabolomic signatures of Cd exposure in insulin-secreting MIN6 cells by using non-target metabolomics. PCA and OPLS-DA analysis clearly suggested that Cd exposure led to a marked metabolic alteration in MIN6 cells. 76 perturbed metabolites were identified after Cd exposure. Classification of metabolites suggested that Cd perturbed metabolites belong to nucleosides, nucleotides and analogues, organic acids and derivatives, and lipids and lipid-like molecules. 28 perturbed metabolites existed in mitochondrion, suggesting mitochondrion as the major target organelle in metabolic toxicity of Cd exposure. KEGG pathway analysis revealed that 20 metabolic pathways were disturbed by Cd exposure. Mitochondrial TCA cycle and glycerophospholipid metabolism were remarkably disturbed. The mRNA expressions of genes in mitochondrial TCA cycle and fatty acid oxidation in pancreas and MIN6 cells were significantly dysregulated by Cd exposure. Disturbances in mitochondrial TCA cycle and glycerophospholipid metabolism result in producing perturbed metabolites in pancreatic β-cells. Moreover, 14 perturbed metabolites identified in MIN6 cells co-existed in the urine of Cd exposed workers. 11 biomarkers of diabetes mellitus were also found to be significantly altered in the urine of Cd exposed workers. In conclusion, findings of this study greatly extend our understanding of metabolic toxicity of Cd exposure in pancreatic β-cells at metabolome-wide level and offer some new clues for linking Cd exposure to development of diabetes mellitus. Results of this study also support the notion that Cd induced metabolic toxicity could be monitored by examining perturbed urinary metabolites in humans and highlight the significance of reducing Cd exposure via drinking water at population level.

Metabolic impact of the VDR rs1544410 in diabetic retinopathy.

To investigate the association between BsmI and DM2 in patients with and without DR and to correlate with clinical parameters in a population in northeastern Brazil. Cross-sectional case-control study in which data were collected from 285 individuals, including 128 patients with DM2 and 157 with DR. Clinical, biochemical and anthropometric parameters were analyzed, in addition to the single nucleotide polymorphism (SNP) BsmI of the VDR gene (rs1544410), genotyped by PCR-RFLP. In the DR group we found a greater number of patients using insulin therapy (p = 0.000) and with longer duration of DM2 (p = 0.000), in addition to higher serum creatinine values (p = 0.001). Higher fasting glucose levels and higher frequency of insulinoterapy were independently observed in patients with DR and b allele carriers, when compared to BB. The association of the bb/Bb genotypes (rs1544410) of the VDR gene with increased blood glucose levels and insulinoterapy may represent worse glicemic control in rs1544410 b allele carriers in DR Latin American individuals.

Assessment of Metabolic Adaptations in Periparturient Dairy Cows Provided 3-Nitrooxypropanol and Varying Concentrate Proportions by Using the GreenFeed System for Indirect Calorimetry, Biochemical Blood Parameters and Ultrasonography of Adipose Tissues

Methanogenesis in ruminants contributes to both greenhouse gas emissions and feed energy losses whereby the latter becomes specifically important in energy-deficient periparturient cows. It was hypothesized that increased concentrate feed proportions (CFP) and feeding with the methane inhibitor 3-nitrooxypropanol (3-NOP), as well as their potential synergism, improve the energy status of peripartal cows. Periparturient dairy cows were fed low or high dietary CFP either tested without or combined with 3-NOP. The GreenFeed system was used to calculate the metabolic respiration quotient (RQmetabolic) and tissue energy retention (ERtissue) by methods of indirect calorimetry. The calorimetrically estimated ERtissue coincided with a conventionally calculated energy balance except for the antepartal period. Neither CFP nor 3-NOP affected the ultrasonographically assessed lipomobilization in adipose depots. In the group fed 3-NOP and a high concentrate feed proportion, the RQmetabolic significantly rose over the course of the experiment and the ERtissue was also increased. Serum non-esterified fatty acid concentrations were lower in the 3-NOP groups albeit ß-hydroxybutyrate (BHB) remained unaffected. Higher CFP reduced BHB and increased blood glucose levels. In conclusion, 3-NOP and high CFP improved the energy budget of the cows in an interactive manner, which was, however, not apparent in all of the examined parameters. The application of the GreenFeed system for indirect calorimetry is a promising approach, which needs further validation in the future.

Anti-Hyperglycemic effect of Polyherbal Formulation in Glucose Loaded and Epinephrine Induced Hyperglycemic Wistar rats

The poly herbal plant extracts were efficient in reducing the elevated glucose level than the independent use of plants. The poly herbal formulation were containing the extracts of Syzgium cumini, Annona squamosa, Momordica charantia, Tinospora cordifolia, Gymnema sylvestre and Curcuma longa. This poly herbal formulation was studied for anti-hyperglycemic activity in the glucose loaded and epinepharine induced hyperglycemic rats. The animal were divided into 5 groups each group had 6 animals; group no 1 was positive control, group no2, 3, 4 were different dose of poly herbal formulation PHF 225 mg/kg, PHF 450 mg/kg and PHF 850 mg/kg respectively and group no 5 was treated by Metformin 500mg/kg. The rats were treated for a period of 7 days, blood glucose level and biochemical parameters were measured on 7thday. The polyherbal formulation significantly (p&lt;0.01) reduced the elevated blood glucose level in epinephrine and glucose induced hyperglycemic rats when compared with positive control group. The PHF has significantly (p&lt;0.05) lower HDL, VDL and cholesterol as compared with the positive control groups. The epinephrine is known to enhance glucagon secretion by α-cells of pancreas and activation of β receptors resulting in an increased glycogenolysis and gluconeogenesis, thereby increases blood glucose level. The active phyto-constituents present in poly herbal formulation are flavonoids and phenolic compounds, triterpene saponins known as gymnemic acids, gymnemasaponins promote the release of insulin and delay the absorption of glucose.

Fluorine MR Imaging Probes Dynamic Migratory Profiles of Perfluorocarbon-Loaded Dendritic Cells After Streptozotocin-Induced Inflammation.

The pathogenesis of type 1 diabetes (T1D) involves presentation of islet-specific self-antigens by dendritic cells (DCs) to autoreactive T cells, resulting in the destruction of insulin-producing pancreatic beta cells. We aimed to study the dynamic homing of diabetes-prone DCs to the pancreas and nearby organs with and without induction of pancreatic stress in a T1D susceptible model of repeated streptozotocin (STZ) injection. In vitro labeling of activated bone marrow-derived DCs (BMDCs) from NOD (Nonobese diabetes) mice was performed using zonyl perfluoro-15-crown-5-ether nanoparticles (ZPFCE-NPs). Internalization of particles was confirmed by confocal microscopy. Two groups of NOD.SCID (nonobese diabetic/severe combined immunodeficiency) mice with (induced by low dose STZ administration) or without pancreatic stress were compared. Diabetogenic BMDCs loaded with BDC2.5 mimotope were pre-labeled with ZPFCE-NPs and adoptively transferred into mice. Longitudinal in vivo fluorine MRI (19F MRI) was performed 24h, 36h and 48h after transfer of BMDCs. For ex vivo quantification of labeled cells, 19F NMR and flow cytometry were performed on dissected tissues to validate in vivo 19F MRI data. In vitro flow cytometry and confocal microscopy confirmed high uptake of nanoparticles in BMDCs during the process of maturation. Migration/homing of activated and ZPFCE-NP- labeled BMDCs to different organs was monitored and quantified longitudinally, showing highest cell density in pancreas at 48-h time-point. Based on 19F MRI, STZ induced mild inflammation in the pancreatic region, as indicated by high accumulation of ZPFCE-NP-labeled BMDCs in the pancreas when compared to the vehicle group. Pancreatic draining lymph nodes showed elevated homing of labeled BMDCs in the vehicle groups in contrast to the STZ group after 72h. The effect of STZ was confirmed by increased blood glucose levels. We showed the potential of 19F MRI for the non-invasive visualization and quantification of migrating immune cells in models for pancreatic inflammation after STZ administration. Without any intrinsic background signal, 19F MRI serves as a highly specific imaging tool to study the migration of diabetic-prone BMDCs in T1D models in vivo. This approach could particularly be of interest for the longitudinal assessment of established or novel anti-inflammatory therapeutic approaches in preclinical models.

Clinical manifestations of polycystic ovary syndrome: a modern view

INTRODUCTION: Polycystic ovary syndrome (PCOS) is one of the urgent problems of gynecological endocrinology. The main signs of PCOS include a violation of menstrual and/or ovulatory function and clinical and/or biochemical hyperandrogenism, as well as polycystic ovarian morphology according to the ultrasound results. PCOS leads to infertility, obesity, and cardiovascular system diseases. PCOS is an important symptom complex in the diagnosis, which directly affects the reproductive function of females, hence the percentage of fertility. This study aimed to analyze the features of clinical, laboratory, and instrumental parameters in females with a previously established PCOS diagnosis referred for surgical treatment. The obtained results were studied from the point of view of the clinical guidelines of the American Society for Reproductive Medicine and the European Society of Human Reproduction and Embryology (2003), International PCOS Network (2018), and the Ministry of Health of the Russian Federation (2021).&#x0D; MATERIALS AND METHODS: The study included 121 females who were admitted to hospitals in Moscow for surgical treatment. Among the gynecological surgical hospitals, the following medical institutions were allocated: the city clinical hospital, the research center, and the commercial clinic we conditionally divided into three levels.&#x0D; The city clinical hospital had 54 females (1st group) aged 2137 years, the research center with 48 females (2nd group) aged 2242 years, and the commercial clinic with 19 females (3rd group) aged 2541. Before the ovarian surgery for PCOS, all patients underwent an additional examination, including the collection of anamnesis, particularly, features of menstrual and generative function, anthropometric indicators, body weight, and height study. Based on these parameters, the body mass index (BMI) was calculated, the clinical signs of hyperandrogenism (acne, excessive hair growth on the body and face, and the degree of hirsutism) were assessed, and the hormonal profile was determined to detect biochemical hyperandrogenism (free testosterone). Additionally, the level of glucose and insulin in the blood plasma was determined in all patients, and a pelvic ultrasound examination (ultrasound) was performed.&#x0D; RESULTS: Menstrual irregularities were revealed, of which complaints were presented by a total of 49.5% of patients. The results of the preliminary selection of patients with PCOS for surgical treatment revealed that 108 (89.2%) had primary and secondary infertility, of whom 64 (52.9%) had BMI within the normative values, 29 (23.9%) were overweight, and 25 (20.6%) were obese. Clinical manifestations of hyperandrogenism were present in 61 (50.4%) patients included in the study. The level of free testosterone above the normative values (2.85 pg/ml) was diagnosed only in 9 (7.4%) patients out of 121 who are included in the study. Additionally, this parameter was within the upper limit of the norm (average value of 2.780.36 pg/ml) in 112 females but was combined with clinical manifestations of hyperandrogenism. Black acanthosis was noted in 6 (5.0%) patients with free testosterone levels in 3.013.64 pg/ml. Increased blood glucose levels were combined with obesity in 3 (5.5%) patients of 1st group and 1 (2.1%) in 2nd group. Insulin levels significantly exceeded the upper limit of the norm in 6 (5.0%) patients. The ultrasound result in all patients (n=121) revealed the presence of echographic signs of PCOS in PCOS was confirmed by ultrasound in 54 females in 1st group, 48 females in 2nd group, and 19 females in 3rd group.&#x0D; CONCLUSION: Our results suggest an unreasonably broad interpretation of PCOS diagnosis. Therefore, PCOS diagnosis should be primarily remembered as a diagnosis-exception. Only repeated, extended, thorough, and comprehensive examination of patients with a presumed PCOS diagnosis will allow you to better navigate the individual characteristics of patients and offer adequate methods for correcting the symptom complex to improve the overall health, fertility, and quality of life.

Expanded substrate specificity supported by P1′ and P2′ residues enables bacterial dipeptidyl-peptidase 7 to degrade bioactive peptides

Dipeptide production from extracellular proteins is crucial for Porphyromonas gingivalis, a pathogen related to chronic periodontitis, because its energy production is entirely dependent on the metabolism of amino acids predominantly incorporated as dipeptides. These dipeptides are produced by periplasmic dipeptidyl-peptidase (DPP)4, DPP5, DPP7, and DPP11. Although the substrate specificities of these four DPPs cover most amino acids at the penultimate position from the N terminus (P1), no DPP is known to cleave penultimate Gly, Ser, Thr, or His. Here, we report an expanded substrate preference of bacterial DPP7 that covers those residues. MALDI-TOF mass spectrometry analysis demonstrated that DPP7 efficiently degraded incretins and other gastrointestinal peptides, which were successively cleaved at every second residue, including Ala, Gly, Ser, and Gln, as well as authentic hydrophobic residues. Intravenous injection of DPP7 into mice orally administered glucose caused declines in plasma glucagon-like peptide-1 and insulin, accompanied by increased blood glucose levels. A newly developed coupled enzyme reaction system that uses synthetic fluorogenic peptides revealed that the P1′ and P2′ residues of substrates significantly elevated kcat values, providing an expanded substrate preference. This activity enhancement was most effective toward the substrates with nonfavorable but nonrepulsive P1 residues in DPP7. Enhancement of kcat by prime-side residues was also observed in DPP11 but not DPP4 and DPP5. Based on this expanded substrate specificity, we demonstrate that a combination of DPPs enables proteolytic liberation of all types of N-terminal dipeptides and ensures P. gingivalis growth and pathogenicity.

MicroRNA Transcriptomics Analysis Identifies Dysregulated Hedgehog Signaling Pathway in a Mouse Model of Acute Intracerebral Hemorrhage Exposed to Hyperglycemia

Objective: Hyperglycemia is often observed in the patients after acute stroke. This study aims to elucidate the potential effect and mechanism of hyperglycemia by screening microRNAs expression in intracerebral hemorrhage mice. Methods: We employed the collagenase model of intracerebral hemorrhage. Twenty male C57BL/6 mice were used and randomly divided in normo- and hyperglycemic. The hyperglycemia was induced by intraperitoneally injection of 50% of Dextrose (8 mL/kg) 3 hours after intracerebral hemorrhage. The neurologic impairment was investigated by neurologic deficit scale. To study the specific mechanisms of hyperglycemia, microRNAs expression in perihematomal area was investigated by RNA sequencing. MicroRNAs expression in hyperglycemic intracerebral hemorrhage animals were compared normoglycemic mice. Functional annotation analysis was used to indicate potential pathological pathway, underlying observed effects. Finally, polymerase chain reaction validation was administered. Results: Intraperitoneal injection of dextrose significantly increased blood glucose level. That was associated with aggravation of neurological deficits in hyperglycemic compared to normoglycemic animals. A total of 73 differentially expressed microRNAs were identified via transcriptomics analysis. Bioinformatics analyses showed that these microRNAs were significantly altered in several signaling pathways, of which the hedgehog signaling pathway was regarded as the most potential pathway associated with the effect of hyperglycemia on acute intracerebral hemorrhage. Furthermore, polymerase chain reaction results validated the correlation between microRNAs and hedgehog signaling pathway. Conclusions: MicroRNA elevated in hyperglycemia group may be involved in worsening the neurological function via inhibiting the hedgehog signaling, which provides a novel molecular physiological mechanism and lays the foundation for treatment of intracerebral hemorrhage.

Lifestyle Modification Therapy in Diabetes Disease: A Review Article

Introduction: Diabetes mellitus (DM) is a metabolic disease characterized by high blood glucose levels (hyperglycemia) resulting from disturbances in insulin secretion, insulin action or both. Insulin is a hormone produced by pancreatic beta cells, which is needed to utilize glucose from digested food. Comprehensive care is taken to treat patients with either prediabetes or diabetes. Diabetes management includes lifestyle interventions along with pharmacologic therapy and routine blood glucose monitoring. So that a decrease in blood glucose can occur and can be stable for a long time. Discussion:Lifestyle modification is an economical treatment that saves costs to prevent or delay the onset of diabetes. On the other hand, nutritional management provided by a dietitian is also recommended. Moderate weight loss goals are an important component of diabetes prevention and treatment, as large body weight can increase blood glucose levels, and can also have an increasing impact on blood pressure and cholesterol levels. Weight loss can be achieved through a balanced diet, with total control of calories and free carbohydrates. However, for diabetic patients following a low-carbohydrate diet, they should be informed about possible side effects such as hypoglycemia, headaches and constipation. Other studies have suggested the consumption of complex dietary fiber and whole grains to improve blood sugar control. Greater adherence to diet combined with light physical activity was associated with a lower likelihood of diabetes after adjusting for various factors. Conclusion:Lifestyle modification is a fairly cost-effective treatment to prevent or delay the onset of diabetes, with a risk reduction of about 58% in 3 years. It is strongly recommended by the ADA that patients with IGT, IFG or HbA1C levels of 5.7-6.4% be counseled on lifestyle changes such as diet and exercise. On the other hand, nutritional management provided by a dietitian is also recommended.

RECENT APPROACHES IN GLUCOSE RESPONSIVE INSULIN DELIVERY SYSTEM

Diabetes mellitus is a chronic medical condition currently affecting 382 million people across the globe, caused due to increased blood glucose levels due to less insulin production or insulin resistance. Subcutaneous insulin administration for diabetes is the only most accepted therapy for maintaining blood glucose levels in diabetic patients. Many patients with advanced type II diabetes mellitus need to regularly monitor their blood glucose level to keep their blood glucose level in the target range. However long-term insulin therapy through an invasive route of administration causes problems with patient compliance and a sudden decrease in blood glucose levels. An artificial closed-loop insulin release system that mimics the glucose-responsive insulin secretion by β-cells of pancreas is one of the ways to overcome the problem faced with the conventional method of insulin administration. Many polymeric formulations showed an improved glucose-responsive release of insulin when incorporated with glucose-responsive catalysts such as glucose oxidase, phenylboronic acid, and glucose binding proteins, the release rate can be controlled by optimizing the concentration of glucose-responsive catalysts. This article is been focused on different mechanisms of glucose-responsive release by incorporation with glucose-responsive catalysts.

Antidiabetic Activity of Piper Longum and Stevia Herbal Formulation

AIM: The aim of the study is to find the antidiabetic activity of piper longum and stevia herbal formulation. INTRODUCTION: Diabetes Mellitus also known as diabetes is a metabolic disorder which is characterised with increased blood glucose level. Frequent urination, increased thirst and increased appetite are the common symptoms. Three types of diabetes are there namely type 1, type 2, and gestational diabetes. Piper longum also known as indian long pepper, belongs to the family piperaceae. It belongs to the indo-malaya region and tropical rainforest region. Stevia rebaudiana commonly known as candly leaf, sweet leaf and sugar leaf belongs to the genus stevia. Stevia is a natural sweetener and natural sugar substitute. MATERIALS AND METHOD: lpha-amylase inhibition was determined by quantifying the amount of maltose liberated during the experiment. Different concentration of nanoparticles (10, 20, 30, 40, 50 L.) was pre-incubated with 100 L of amylase solution (1U/mL) at room temperature for 30 minutes. DNSA reagent was added to stop the reaction and the solution was heated in a water bath for 5 minutes. Control was maintained where the equal quantity of enzyme extract was replaced by sodium phosphate buffer maintained at a pH value of 6.9. RESULTS AND DISCUSSION: The bar graph represents the antidiabetic activity of piper longum and stevia herbal formulation in various concentrations in L. 10 %L of concentration shows 45% of inhibition, 20 % L of concentration shows 50% of inhibition, 30% of concentration shows 65% of inhibition, 40% of concentration shows 70% of inhibition and 50 %of concentration shows 80% of inhibition. CONCLUSION: From this study it is clear that the fruit of piper longum and stevia leaves has proven to be a better choice for antidiabetic activity.

Therapeutic Use of Glucagon: The Future of Diabetes Mellitus Therapy

The etiology of type 2 diabetes has long been centered on insulin. Recently, however, glucagon has been attracting attention. It has been reported that glucagon receptor or pancreatic α cell-deficient mice do not deteriorate glucose intolerance after pancreatic beta cells are destroyed with streptozotocin and insulin secretion is depleted. This suggests that glucagon and glucagon receptors are crucial for glucose intolerance in diabetes mellitus, and the elucidation of glucagon signaling is still awaited. This review will begin with an overview of the history of glucagon. In the nearly 100 years since the discovery of glucagon, it has become clear that glucagon plays a role in blood glucose in type 2 diabetes as much or more than insulin. This is due in large part to the advent of the sandwich Enzyme-Linked Immunosorbent Assay (ELISA) method, which allows accurate measurement of glucagon by using N- and C-terminal antibodies. Next, the mechanism of action of glucagon on blood glucose levels will be outlined. Glucagon increases blood glucose levels by enhancing gluconeogenesis and glycogenolysis. Glucagon also enhances lipolysis, which indirectly contributes to weight loss and improved blood glucose levels. Recently, mice with defective or decreased glucagon secretion have been developed, and the effects of glucagon are becoming clearer in vivo. Mice with elevated glucagon secretion have also appeared, and their glucose tolerance improvement through lipolysis and weight loss is also attracting attention. Research on glucagon is not limited to animal experiments but has already reached the stage of clinical application. Drugs that suppress glucagon have side effects such as liver damage, but they are effective in improving blood glucose. Drugs that increase glucagon also have hypoglycemic effects resulting from weight loss. Thus, glucagon has undergone a dizzying evolution over the past decade. The day may soon come when drugs that target glucagon will dominate diabetes treatment.

Sufficiently Elevated Core Body Temperature May Be Necessary to Maintain Cerebral Blood Flow Response throughout the Morning

In comparison to a carbohydrate-rich breakfast, a nutritionally balanced breakfast reportedly leads to a higher core body temperature because of diet-induced thermogenesis (DIT) and also results in higher task performance. This study aimed to examine the relationships among the core body temperature, blood glucose level, cerebral blood flow, and cognitive performance when the core body temperature is raised to a similar extent as in DIT in the morning. This crossover study included 18 male participants who performed four sets of cognitive tests in the morning with four different foot baths and glucose intake conditions. In elevated body temperature (EBT) conditions, the core body temperature was increased by a foot bath at 42˚C or 39˚C, while in low body temperature (LBT) conditions, it was maintained at 35˚C by a foot bath; the participants received no glucose or two intakes of 20-g glucose for each thermal condition. In addition to the core body temperature measurement, the cerebral blood flow in the dorsolateral prefrontal cortex (DLPFC) was measured using near-infrared spectroscopy. Three blood collections were performed to measure the changes in blood glucose levels. The results revealed that in the EBT conditions, the core body temperature remained 0.3˚C - 0.5˚C higher than that at wake-up time, while the glucose intake conditions increased blood glucose levels which remained higher than those during fasting. No significant between-treatment difference was observed in the results of cognitive tests. However, the blood flow in the DLPFC increased during the second test period in the EBT/glucose and LBT/glucose conditions, whereas during the fourth test period, it increased solely in the EBT/glucose condition. Thus, in addition to the blood glucose level, an elevated core body temperature within the physiological range may be needed for long-term maintenance of the cerebral blood flow response.

The Relationship between Microalbumin Levels and HbA1c in People at Risk for Type 2 Diabetes Mellitus

Increased blood glucose levels in diabetes mellitus are a problem due to poor insulin synthesis, and insulin resistance conditions cause microalbumin. The purpose of this study was to determine the relationship between microalbumin levels and HbA1c in people at risk for type 2 diabetes mellitus. This type of research is a quantitative study using a cross-sectional study design. Respondents in this study were all women at risk of type 2 diabetes mellitus in the village of Ngrandulor. The research sample was taken using the purposive sampling technique, which was taken according to the criteria set by the researcher so that 28 respondents were selected. Data collection on microalbumin levels was carried out by immunoturbidimetric examination, and HbA1c was carried out by immunoassay examination. Bivariate analysis of the research data was carried out using the Spearman rank statistical test. The results showed a relationship between microalbumin levels and HbA1c in people at risk for type 2 diabetes mellitus, the p-value = 0.010. The results showed that half of the respondents had their albumin checked under microalbumin conditions, namely 14 respondents (50%) and on HbA1c examination, which showed that they were in diabetes status or conditions, namely 14 respondents (50%). This study concludes that there is a relationship between microalbumin levels and HbA1c in people at risk for type 2 diabetes mellitus, with a significance value (p) of 0.010.

Kombinasi Ekstrak Etanol 96% Daun Teh (Camellia sinensis (L.) Kuntze) dan Daun Yakon (Smallanthus sonchifolius) sebagai Penghambat Enzim ?-glukosidase

Diabetes mellitus is a degenerative disease in the form of metabolic disorders characterized by increased blood glucose levels and impaired insulin metabolism. Yakon leaves and tea leaves contain phenolic compounds which have ?-glucosidase inhibiting activity. The aims of this study was to determine the inhibitory activity of the ?-glucosidase enzyme from tea leaf and yakon leaf extracts and the combination of the two plants. Extraction of the active compounds contained in tea leaves and yakon leaves was carried out by graded maceration method using n-hexane, ethyl acetate, 96% ethanol and water as solvents (using the decoction method). Phytochemical analysis of 96% ethanol extract of tea leaves and yakon leaves contained alkaloids, flavonoids, tannins, saponins, triterpenoids/steroids and phenols. The results of testing the inhibitory activity of the -glucosidase enzyme showed that the IC50 values ??for 96% ethanol extract of tea leaves and yakon leaves were 22.31 and 19.70 ppm, respectively. Combination of 96% ethanol extract of tea leaves and yakon leaves in a ratio of 1:1; 1:3; 3:1 obtained IC50 of 64.73 ppm respectively; 7.34 ppm, and 11.03 ppm.

PENGARUH PEMBERIAN COOKIES TEPUNG LABU KUNING DAN KEDELAI TERHADAP PENURUNAN KADAR GLUKOSA DARAH PADA PASIEN DIABETES MELLITUS TIPE II

Introduction Diabetes Mellitus is a chronic disease caused by reduced insulin production from the pancreas and insulin produced is not effective in reducing blood sugar levels. This situation will increase blood glucose levels. Alternative therapy management to control blood glucose by utilizing food ingredients that can help reduce blood sugar levels, one of which is pumpkin flour and soybeans. The purpose of this study was to determine the effect of giving pumpkin flour cookies to a decrease in blood sugar levels in Type 2 DM patients in Palembang Social Health Center. Method is a quasi-experimental research design with a pretest-posttest with control group using a comparison group. The subjects of this study were 76 people with Type 2 Diabetes Mellitus in the Social Health Center who were willing and fulfilled the inclusion and exclusion criteria. Data analysis uses t-dependent and t-independent tests. Results showed that there was an average difference in the determination of blood sugar levels in the treatment group at 59.89 mg / dl and in the comparison group at 17.89 mg / dl. The t-independent statistical test results obtained p-value &lt;0.05 (0,000), that there was an Effect of Provision of Yellow Pumpkin Flour Cookies and Soybeans on Decreasing Blood Sugar Levels in Type 2 Diabetes Mellitus Patients in Palembang Social Health Center. Conclusion There Is An Effect Of Provision Of Pumpkin Flour Cookies And Soy To Lower Blood Sugar Levels In Type 2 Diabetes Mellitus Patients in Palembang Social Health Center.

The activation of hypothalamic AMP-activated protein kinase by oxidative stress is related to hyperphagia in diabetic rats.

During diabetes, there are increased blood glucose levels and oxidative stress. The relationship between oxidative stress and the phosphorylation of AMP-activated protein kinase at the hypothalamic level has been little studied. The objective of this study was to analyze the relationship between oxidative stress and AMP-activated protein kinase activation in Wistar rats with hyperphagia and hyperglycemia. Rats at 7, 14, and 28 days with diabetes were used. Control rats were included. Food intake was calculated to determine hyperphagia. The hypothalamus was extracted to evaluate oxidative stress markers by spectrophotometry; phosphorylation of AMP-activated protein kinase, growth hormone receptor 1a, and neuropeptide Y expression were determined by Western blot. There was a significant increase in the consumption of food in the experimental groups. The level of malondialdehyde decreased in the 7-day group (33%) and increased significantly in the 28-day group (90%), glutathione peroxidase activity increased in the 7-day group (70%) and decreased in the 28-day group (34%), and the phosphorylation of AMP-activated protein kinase increased significantly in the 28-day group (86%). Under ex-vivo conditions in animals with 28 days of hyperglycemia, glutathione peroxidase activity increased 195%, the malondialdehyde level decreased 87%, phosphorylation of AMP-activated protein kinase decreased 53%, and growth hormone receptor 1a expression decreased 66%, when treating hyperglycemic hypothalamic tissue with an antioxidant. NPY expression increased in hyperglycemia, and antioxidant treatment did not regulate its expression. The activation of AMP-activated protein kinase is related with an increase in oxidative stress markers in hyperglycemic and hyperphagic rats.

Circadian Liver Metabolism Affects Management of Type 2 Diabetes Mellitus During Cytokine Storm Due to COVID-19

Diabetes is managed to keep the blood sugar in normal range. This involves liver as glucose metabolizing organ and sensitization of somatic cells to utilize this glucose for daily energy requirements. The management is subjected to the rhythmic glucose intake as diet and liver circadian cycles that runs parallel to this zeitgeber. COVID-19 patients having diabetes as comorbid condition face the challenges of inflammatory cytokine management along with the organization of glucose. Increased blood glucose level during the cytokine storm further aggravates the pathophysiology of COVID-19 patients leading to high morbidity and mortality in such patients. Clinical treatment of these patients requires multidimensional approach involving circadian variation of hepatic physiology, glucose intake, and inflammatory cytokine release.