Anti-Hyperglycemic effect of Polyherbal Formulation in Glucose Loaded and Epinephrine Induced Hyperglycemic Wistar rats

Abstract

The poly herbal plant extracts were efficient in reducing the elevated glucose level than the independent use of plants. The poly herbal formulation were containing the extracts of Syzgium cumini, Annona squamosa, Momordica charantia, Tinospora cordifolia, Gymnema sylvestre and Curcuma longa. This poly herbal formulation was studied for anti-hyperglycemic activity in the glucose loaded and epinepharine induced hyperglycemic rats. The animal were divided into 5 groups each group had 6 animals; group no 1 was positive control, group no2, 3, 4 were different dose of poly herbal formulation PHF 225 mg/kg, PHF 450 mg/kg and PHF 850 mg/kg respectively and group no 5 was treated by Metformin 500mg/kg. The rats were treated for a period of 7 days, blood glucose level and biochemical parameters were measured on 7thday. The polyherbal formulation significantly (p<0.01) reduced the elevated blood glucose level in epinephrine and glucose induced hyperglycemic rats when compared with positive control group. The PHF has significantly (p<0.05) lower HDL, VDL and cholesterol as compared with the positive control groups. The epinephrine is known to enhance glucagon secretion by α-cells of pancreas and activation of β receptors resulting in an increased glycogenolysis and gluconeogenesis, thereby increases blood glucose level. The active phyto-constituents present in poly herbal formulation are flavonoids and phenolic compounds, triterpene saponins known as gymnemic acids, gymnemasaponins promote the release of insulin and delay the absorption of glucose.