Improve Milk Yield Research Articles

Effects of Propylene Glycol on Negative Energy Balance of Postpartum Dairy Cows.

Simple SummaryAfter calving, the milk production of dairy cows increases rapidly, but the nutrient intake cannot meet the demand for milk production, forming a negative energy balance. Dairy cows in a negative energy balance have an increased risk of developing clinical or subclinical ketosis. The ketosis in dairy cows has a negative impact on milk production, dry matter intake, health, immunity, and reproductive performance. Propylene glycol can be used as an important gluconeogenesis in ruminants and can effectively inhibit the formation of ketones. Supplementary propylene glycol to dairy cows during perinatal is an effective method to alleviate the negative energy balance. This review summarizes the reasons and consequences of negative energy balance as well as the mechanism and effects of propylene glycol in inhibiting a negative energy balance in dairy cows. In addition, the feeding levels and methods of using propylene glycol to alleviate negative energy balance are also discussed.With the improvement in the intense genetic selection of dairy cows, advanced management strategies, and improved feed quality and disease control, milk production level has been greatly improved. However, the negative energy balance (NEB) is increasingly serious at the postpartum stage because the intake of nutrients cannot meet the demand of quickly improved milk production. The NEB leads to a large amount of body fat mobilization and consequently the elevated production of ketones, which causes metabolic diseases such as ketosis and fatty liver. The high milk production of dairy cows in early lactation aggravates NEB. The metabolic diseases lead to metabolic disorders, a decrease in reproductive performance, and lactation performance decline, seriously affecting the health and production of cows. Propylene glycol (PG) can alleviate NEB through gluconeogenesis and inhibit the synthesis of ketone bodies. In addition, PG improves milk yield, reproduction, and immune performance by improving plasma glucose and liver function in ketosis cows, and reduces milk fat percentage. However, a large dose of PG (above 500 g/d) has toxic and side effects in cows. The feeding method used was an oral drench. The combination of PG with some other additives can improve the effects in preventing ketosis. Overall, the present review summarizes the recent research progress in the impacts of NEB in dairy cows and the properties of PG in alleviating NEB and reducing the risk of ketosis.

Reproductive Responses, Metabolic Disorder, Ruminal Fermentation Characteristics, and Milk Production of Postpartum Holstein Cows Fed Steam Flaked of Corn and Barley Grains

This study evaluated the effects of steam flaked of corn and barley grains on the reproductive response, ruminal fermentation characteristics, metabolic disorder, and milk production of postpartum Holstein cows. A total of 40 postpartum Holstein dairy cows were divided into 4 groups (n=10) and fed 1) ground grains (control), 2) steam flacked corn+milled barley, 3) steam flacked barely+milled corn, and 4) steam flaked corn and barley. The animals were treated for 0-30, 30-60, 60-90, and 90-120 days post-calving. Milk yield and compositions, total conception rate, endometritis, pyometra, ruminal fermentation characteristics, ketosis, and acidosis were assessed. This study was conducted in a completely randomized design. The results showed that dietary inclusion of steam flaked grains improved milk yield and compositions during the experimental period compared to the control group (p<0.05), except for cows treated for 0-30d days post-calving. Cows fed steam flaked grains showed a lower occurrence of reproduction and metabolic disorders compared to the control group (p<0.01). Cows fed a combination of steam flaked grains showed better pregnancy rates in the first 100 days and open days of postpartum cows compared to cows in control and a single form of steam flaked barley or corn (p<0.05). Total VFA, acetate, propionate, butyrate, and valerate were higher in cows fed steam flaked compared to the control group (p<0.05). Feeding steam flaked grains is advised for improving reproductive performance, milk variables, and rumen fermentation profiles in dairy cows.

Mineral mixture supplementation for enhancing milk yield of milch animals in Haryana

Most of the Indian soils are deficient in one or more minerals and these minerals are important in numerous biochemical reactions and deficiency may lead to metabolic diseases resulting lowering production performance in dairy animals. The study attempts to evaluate status nutrient intake in dairy animals, effect of reappropriation of available feed resources and mineral mixture supplementation on production performance in lactating cows and buffaloes in Katesara and Amarpur villages under Farmers’ FIRST project in Palwal district. The animals were in their early lactation (40±5 days in milk) and milk yield, its composition, body weight and body condition score was recorded. Among the cow, 28% deficit, 35% excess dry matter; 41% deficit and 11% excess crude protein; 33% deficit and 25% excess total digestible nutrients (TDN) was provided. In buffaloes, 36% deficit and 40% excess DM; 45% deficit and 15% excess CP; 25% deficit and 12% excess TDN was provided. Reappropriation of DM, CP and TDN was done using available feed/ fodders available to the farmers and after 15 days, mineral mixture was provided @ 50 g/d for 60 days. The production performance record reveals no change in body weight but BCS was improved. Milk yield, fat, protein in cows were improved and other composition were remain unchanged. In buffaloes, milk yield, fat and protein improved while others, viz. lactose, SNF, ash and TS were similar due to supplementation of mineral mixture. The results conclude that imbalance of nutrients existed to the tune of 10 to 45% among various nutrients. One of the best propositions could be supplementation of mineral mixture to improve milk yield (10 to 14%) and fat per cent under field condition in both cows and buffaloes.

Association of GHR Polymorphisms with Milk Production in Buffaloes.

Simple SummaryThe present study reported two missense mutations in the buffalo GHR gene: A novel (c.380G>A) and (c.836T>A) which was described in previous studies. These two single nucleotide polymorphisms (SNPs) were found to be associated with milk yield, fat %, protein %, and 305 day-milk, fat and protein yield, with higher performance for AA haplotype animals. Therefore, selection of buffaloes with AA haplotype would more likely improve milk production traits. Consequently, this would allow breeders to take more precise selection decisions, leading to significantly higher productivity and profitability within the Egyptian buffalo herds.For its role in the mediation of growth hormone (GH) galactopoietic effect, growth hormone receptor (GHR) was considered a functional candidate gene for milk performance in cattle. However, its genetic variation and potential effect have not been investigated in Egyptian buffaloes. This study aimed to screen GHR for polymorphisms and study their associations with milk traits in Egyptian buffaloes. Polymerase chain reaction, single-strand conformation polymorphism, and sequencing were used to identify mutations in 4 exons (E4–E6 and E8) of the GHR gene in 400 Egyptian buffaloes. No polymorphisms were found in E4, while 2 SNPs (c.380G>A/p.Arg127Lys and c.387C>T/p.Gly129) in E5, one silent mutation (c.435A>G/p.Pro145) in E6, and another missense mutation (c.836T>A/p.Phe279Tyr) in E8 were detected. The c.380G>A SNP in the extracellular domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher levels in animals carrying the mutant A allele. The c.836T>A SNP in the transmembrane domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher milk yield and lower fat %, protein %, fat and protein yield in the mutant A allele-animals. Interestingly, animals with the two mutant AA alleles produced higher milk yield, fat %, protein %, fat and protein yield, accompanied with upregulated expressions of GHR, GH, insulin-like growth factor 1 (IGF1), prolactin (PRL), prolactin receptor (PRLR), β-casein (encoded by CSN2 gene), and diacylglycerol acyltransferase-1 (DGAT1) genes and proteins in milk somatic cells. Therefore, selection of Egyptian buffaloes with mutant AA haplotypes for the novel c.380G>A SNP and the well-known c.836T>A SNP could improve milk yield and quality in buffaloes.

Innovation in Tigernut (Cyperus Esculentus L.) Milk Production: In Situ Hydrolysis of Starch.

Tigernut tubers (Cyperus esculentus) are used for the production of vegetable milk, commonly known as “Horchata de chufa” in Spain. The presence of starch in the tuber limits the yield of the milk, since this carbohydrate gelatinizes during the pasteurization of the milk and leads to the considerable solidification of this drink. The present work aims to improve the yields and extraction practice of the milk by an in situ hydrolysis of starch, using exogenous amylases of industrial or vegetable origin. The obtained results show that sprouting improves the extraction yields of tigernut milk, which goes from 50% to about 70%. This improvement in milk yield corresponds to a hydrolysis of about 35% of the starch in the tuber. The use of exogenous amylases leads to starch hydrolysis rates of 45% and 70%, respectively, for amylolytic extracts from sprouted tigernut tubers and amylase, with the corollary of a natural increase in the sweetness of milk. This technical approach makes it possible to produce a naturally sweetened tigernut milk which easily lends itself to pasteurization without a significant increase in viscosity.

Effect of Feeding Improved Grass Hays and Eragrostis Tef Straw Silage on Milk Yield, Nitrogen Utilization, and Methane Emission of Lactating Fogera Dairy Cows in Ethiopia.

Simple SummaryIn tropical regions, it is common practice to feed dairy cows poor-quality roughage, but this diet has been shown to decrease animal productivity and increase methane emissions. For these reasons, introducing alternative roughage feeds, such as nutritionally improved forages or bio-chemically treated straw, is essential for improving milk yield, dietary nitrogen utilization, and reducing enteric methane emission from dairy cows. Thus, we evaluated the effects of natural pasture hay, two improved grass hays (Napier and Brachiaria hybrid grasses), and treated teff straw silage feeding as basal diets on nutrient digestibility, milk yield, nitrogen utilization efficiency, and enteric methane emissions using lactating Fogera dairy cows. Our results showed that improved grass hays and treated teff straw silage diet feeding increased milk yield, nutrient digestibility, and nitrogen utilization efficiency as compared to natural pasture hay. Moreover, the cows fed with improved grass hays and treated teff straw resulted in changing the nitrogen excretion pathway from urine to feces, as well as reduction of the methane production per daily milk yield. Hence, these results provide a novel feeding regimen through feeding nutritionally upgraded forages as a basal diet, which improves milk yield, nutrient utilization efficiency, and reduction of methane emission for sustainable dairy production in tropical regions.The nutritionally imbalanced poor-quality diet feeding is the major constraint of dairy production in tropical regions. Hence, alternative high-quality roughage-based diets are required to improve milk yield and reduce methane emission (CH4). Thus, we tested the effects of feeding natural pasture hay, improved forage grass hays (Napier and Brachiaria Hybrid), and treated crop residues (Eragrostis tef straw) on nutrient digestibility, milk yield, nitrogen balance, and methane emission. The eight lactating Fogera cows selected for the experiment were assigned randomly to a 4 × 4 Latin square design. Cows were housed in well-ventilated individual pens and fed a total mixed ration (TMR) comprising 70% roughage and 30% concentrate. The four roughage-based basal dietary treatments supplemented with formulated concentrate were: Control (natural pasture hay (NPH)); treated teff straw silage (TTS); Napier grass hay (NGH); and Brachiaria hybrid grass hay (BhH). Compared with the control diet, the daily milk yield increased (p < 0.01) by 31.9%, 52.9%, and 71.6% with TTS, NGH, and BhH diets, respectively. Cows fed BhH had the highest dry matter intake (8.84 kg/d), followed by NGH (8.10 kg/d) and TTS (7.71 kg/d); all of these intakes were greater (p = 0.01) than that of NPH (6.21 kg/d). Nitrogen digestibility increased (p < 0.01) from the NPH diet to TTS (by 27.7%), NGH (21.7%), and BhH (39.5%). The concentration of ruminal ammonia nitrogen was higher for cows fed NGH than other diets (p = 0.01) and positively correlated with plasma urea nitrogen concentration (R² = 0.45). Feeding TTS, NGH, and BhH hay as a basal diet changed the nitrogen excretion pathway from urine to feces, which can help protect against environmental pollution. Estimated methane yields per dry matter intake and milk yield were decreased in dairy cows fed BhH, NGH, and TTS diets when compared to cows fed an NPH diet (p < 0.05). In conclusion, feeding of TTS, NGH, and BhH roughages as a basal diet to lactating dairy cows in tropical regions improved nutrient intake and digestibility, milk yield, nitrogen utilization efficiency, and reduced enteric methane emission.

Intake of Spineless Cladodes of Opuntia ficus-indica During Late Pregnancy Improves Progeny Performance in Underfed Sheep.

Simple SummaryPlants in the Opuntia genus are abundant and can be used as a feed supplement because they are highly digestible and can provide water and energy. We fed sheep during late gestation with alfalfa (Control), Opuntia (Opuntia) or protein-enriched Opuntia (E-Opuntia) and measured milk yield and postnatal growth in the progeny. Birth weight did not differ among treatments (p > 0.05) but progeny from E-Opuntia grew faster (p < 0.01) and were heavier at weaning (p < 0.05), despite the fact that Control ewes produced more milk (p < 0.05). Feeding ewes with Opuntia (protein enriched or not) during the last third of gestation is an option for reducing production costs in underfed females managed under extensive conditions in arid and semiarid regions.The present study tested whether feeding ewes during the last third of pregnancy with cladodes of Opuntia (untreated or protein-enriched), as an alternative to alfalfa hay, would improve milk yield as well as the pre- and post-natal growth of their lambs. Sixty mature Rambouillet ewes and their progeny were randomly allocated among three nutritional treatments: (i) Control, fed alfalfa; (ii) Opuntia, fed untreated cladodes; (iii) E-Opuntia, fed protein-enriched cladodes (pre-treated with urea and ammonium sulphate). Birth weight did not differ among treatments (p > 0.05) but Control ewes produced more milk than both groups of Opuntia-fed ewes (p < 0.05). However, milk yield was not related to the growth of the progeny (p > 0.05) because lambs from E-Opuntia-fed ewes grew faster (p < 0.01) and were heavier at weaning (p < 0.05) than lambs from the other two groups. We conclude that Opuntia (with or without protein enrichment) can be used as an alternative to alfalfa hay for feeding ewes during the last third of pregnancy and therefore reduce production costs under extensive conditions in arid and semiarid regions. Moreover, protein-enriched Opuntia appears to improve postnatal lamb growth.

Effect of Supplement Feeding of Shatavari on Lactating Buffaloes

A study was conducted in adopted villages of KVK Bundi to assess the effect of supplementing shatavari (Asparagus racemosus) feed on performance of lactating buffaloes. Twenty lactating buffaloes in mid lactation with similar age, body weight and milk yield were selected from dairy farms of the local farmers and divided into two groups of ten animals each. Control group was maintained as per farmer's practices (feeding of 3 kg cotton seed cake along with wheat straw), while groups comprising feeding of wheat straw + shatavari powder 50gm /day /animal up to 60 days. Result revealed that 19.67 per cent higher average milk yield was recorded in buffaloes feeding under group (7.36 kg/day) as compared to (6.15 kg/day). Increase in the income by feeding Shatavari came to Rs. 50.50/day/animal. It was concluded that feeding of Shatavari along with wheat straw improves milk yield and fat percent as well as reproductive performance in lactating buffaloes.

Effect of feeding an immune modulator to multiparous Holstein cows during the dry period and early lactation on health, milk and reproductive performance

In this study we evaluated the effect of feeding an immune modulatory feed additive (OmniGen-AF®; OMN) during the dry period through early lactation on postpartum health, subsequent lactational and reproductive performance of multiparous Holstein cows. We hypothesized that feeding OMN beginning in the dry period and through 150 days in milk (DIM) would improve milk yield and reproductive performance as a result of improved immune response. To test our hypothesis, 1392 multiparous pregnant Holstein cows of a commercial dairy herd were enrolled and randomly assigned at dry-off (47.6 ± 9.4 d before calving) to OMN (n = 706) or control (CON, n = 686) treatments. Both OMN and CON (placebo) supplements were fed once per day at a rate of 56 g/d per head from dry-off through 150 DIM. Cows were milked three times a day and daily milk yield was measured once per week, starting at the second week of lactation through 140 days in milk. Postpartum health disorders were monitored daily throughout the experiment. Cows were bred following a double OvSynch protocol after a 77-d voluntary waiting period (VWP). Final pregnancy status was assessed after supplementation ended at 230 DIM. Our results indicated that cows supplemented with OMN produced more milk than CON (40.8 kg/d vs. 40.1 kg/d; P < 0.01) during the first 140 d of lactation. Once they entered lactation, cows supplemented with OMN tended (P < 0.10) to have a reduced incidence of mastitis, retained placenta, displaced abomasum (P < 0.11) and reduced number of days spent in the hospital pen relative to CON. OmniGen feeding was also associated with a 10-d reduction in days open (P < 0.05) compared to CON. However, pregnancy rate at first, second and until ∼230 DIM did not differ between treatments. In conclusion, supplementing an immunomodulatory feed additive to dry and early lactation dairy cows was associated with improved milk yield, a tendency to reduce transition cow disorders and reduced number of days open.

Efecto de subproductos de palma africana en la producción y calidad de leche bovina en el sur del departamento del Atlántico, Colombia

El objetivo del presente trabajo fue evaluar el efecto de subproductos de la palma africana (Elaeis guineensis Jacq.) en la producción y la calidad de la leche bajo condiciones de pequeño productor doble propósito en el sur del departamento del Atlántico, Colombia. Para ello, se estableció un diseño de sobrecambio, balanceado para efectos residuales en seis fincas en Repelón (experimento i), y dos fincas en Manatí y cuatro en Campo de la Cruz (experimento ii). Se suplementó durante un periodo de acostumbramiento y evaluación, torta de palmiste (pkc) y decantado de lodos (opdc) como dietas experimentales, y se comparó con sal mineralizada (SalM). Las dietas afectaron (p &lt; 0,05) la producción de leche. El opdc registró la mayor producción de leche (3,20 y 4,01 L/vaca/día en experimentos i y ii, respectivamente), sin diferencia (p &gt; 0,05) con el pkc, aunque superior (p &lt; 0,05) al tratamiento con SalM (2,91 y 3,38 L/vaca/día en experimentos i y ii, respectivamente). La calidad composicional de la leche fue afectada por las dietas evaluadas (p &lt; 0,05). Las dietas afectaron la concentración de nitrógeno ureico en leche (p &lt; 0,05), indicando posibles alteraciones en la relación de proteína degradable en rumen y carbohidratos fermentables. El opdc y el pkc se presentan como suplementos promisorios para mejorar la producción de leche bajo condiciones de pequeño productor en el sur del Atlántico.

Effects of Silymarin on milk production, liver enzymes, oxidative status and HSP70 gene expression in postparturient Sanjabi ewes

In the sheep farming industry, breeders need suitable strategies in order to improve milk yields. Meanwhile, silymarin (a natural hepatoprotector substance) has beneficial effects on common oxidative stress at the beginning of lactation. This study was the first research to evaluate the effect of silymarin on milk production, liver enzymes, oxidative and HSP70 responses in the postpartum period. Total 20 Sanjabi ewes were divided into two groups: control (group C: no addition) and treated (group T: received a diet supplemented with silymarin at 2000 mg/kg feed for 15 d after lambing). Data indicated that silymarin reduced postpartum body weight(BW) loss. At the same time, the feed intake (FI) rate increased. In addition, the peaks of milk yields could be achieved earlier compared with control ewes (P&lt; 0.05). Treatment decreased milk compositions (fat and protein) on days 10 and 15. Furthermore, the reactive oxygen species (ROS) and malondialdehyde (MDA) contents in group T were significantly lower than group C (P&lt; 0.05). Also, the activities of endogenous antioxidant enzymes (Glutathione peroxidase, Superoxide dismutase, and Catalase) were increased. Silymarin remarkable increased the serum alanine aminotransferase (ALT) values (P&lt; 0.05). Meanwhile, an increasing trend in the total protein levels was recorded in group T as compared to group C (P&gt; 0.05). The QRT-PCR analysis showed that silymarin supplemental reduced expression of HSP70 gene in blood serum (P&lt; 0.05). It means that changing the diet can affect the activity of heat shock proteins that consequently changes the quality of animal products. In conclusion, our observation relieved that silymarin treatment in the puerperium periodis potentially an effective strategy to improve milk quality via dual hep at oprotective and antioxidant functions.

Effect of carbohydrate source on productive performance, ruminal and systemic health of grazing cows

Background: The nutritional limitations of Cenchrus clandestinus –i.e., high protein and low energy concentrations- make it necessary to supplement cows with non-fibrous carbohydrate (NFC) sources to improve productive performance. Nevertheless, such supplementation can lead to ruminal acidosis. Objective: To evaluate partial replacement of corn grain (Zea mays, ZM) with sorghum grain (Sorghum vulgare, SV), cassava root (Manihot esculenta, MES) or citrus pulp (Citrus sp., C) on milk yield and quality, ruminal pH and health of grazing cows. Methods: Eight Holstein cows were evaluated in a 4 x 4 Latin square design during the first 60 days of lactation. Treatments (isoenergetic rations, 1.45 ± 0.003 Mcal NEL/kg DM) consisted of a mixture of grass and four concentrates with different NFC sources. Results: No differences in dry matter intake, feed efficiency, ruminal pH, hematological and metabolic profile were observed between treatments. Rumen pH was higher than 6.0, confirming the absence of ruminal acidosis. Milk yield (energy-corrected), protein, and total solids were higher for MES vs. C. Conclusions: None of the NFC sources tested compromised the ruminal or systemic health of the cows, while MES improved milk yield and quality.

IoT for Development of Smart Dairy Farming

Internet of things (IoT) and data-driven techniques are creating greater opportunities for smart dairy farming. The demand for milk is continuously increasing due to increasing population of the world. The consumption of the dairy products is more in developed countries as compared to developing countries. To meet this increased demand for milk products, better technological techniques for improving milk yield are required. It is expected that the use of IoT and different AI techniques can assist a farmer to overcome different traditional farming challenges and increase the milk production. In this research, the authors address different challenges that a dairy farmer has to face in daily life. Brief introduction of smart dairy farming (SDF) is presented with respect to the innovation in production and the processes of smart dairy farming. This review focuses on different aspects of smart dairy farming, and finally a state-of-the-art framework that can assist the farmers to increase the milk yield by using different latest technologies has been proposed. These technological methods can decrease the factors negatively affecting milk production and increase those positively affecting production with minimal resources.

Phenotypic Characterization of Milk Yield and Quality Traits in a Large Population of Water Buffaloes.

Simple SummaryThe buffalo dairy industry has deep roots in Southern Italy, due to the traditional link with Mozzarella di Bufala, a Protected Designation of Origin cheese with high economic value and market demand. At farm level, strategies aiming to improve milk yield and quality are essential to maximize profitability and dairy chain efficiency. In this study, we analyzed a large data set of Italian Buffaloes (around 70 thousands animals) in order to detect the phenotypic sources of variation of milk yield and quality traits, disclose exploitable favorable correlations among milk traits, and provide useful information for dairy buffalo chain stakeholders.The buffalo milk industry has economic and social relevance in Italy, as linked to the manufacture of traditional dairy products. To provide an overview of the current status of buffaloes’ performances on a large scale, almost 1 million milk test-day records from 72,294 buffaloes were available to investigate milk yield, energy corrected milk, fat, protein, and lactose content, and somatic cell score (SCS). Phenotypic correlations between milk traits were calculated and analysis of variance was carried out through a mixed model approach including fixed effect of parity, stage of lactation, sampling time, month of calving, and all their interactions and random effects of buffalo, herd-test-date, and residual. Third-parity buffaloes were the most productive in terms of milk yield, while the lowest solid content was detected in sixth parity buffaloes. A considerable gap between primiparous and multiparous buffaloes was observed for milk yield, especially in early- and mid-lactation. Overall, SCS progressively increased with parity and showed a negative correlation with milk yield in both primiparous (−0.12) and multiparous (−0.14) buffaloes. Results suggested that, at the industrial level, milk of primiparous buffaloes may be preferred for transformation purposes, since it was characterized by greater solid content and lower SCS. Results of this study provide a picture of the Italian population of buffaloes under systematic performance records and might be beneficial to both dairy industry and breeding organizations.

β-carotene Supplementation Does Not Improve Milk Yield and Milk Components of Saanen Goats

We hypothesized that supplementing goats with β-carotene would increase milk yield and improve milk quality. Therefore, the current study aimed at evaluating the effect of supplemental β-carotene on milk yield and milk components. Goats were divided into two groups, one group received β-carotene 100 and 50 mg/head/day before and during the drying off period, respectively, while the second group received water as placebo with similar quantities. Colostrum samples were collected for three days and the milk samples were collected once a week for a month. Milk yield, protein%, fat%, lactose% and Somatic Cell Counts (SCC) were analysed. Milk fat, lactose and protein were analysed using Spectrometer and SCC was analysed using Cytometer. The data collected for milk yield, fat, protein and lactose and SCC were analysed using one-way analysis of variance for repeated measures with PROC GLM procedure of SAS (version 9.4). The study found no effect of supplemental β-carotene on milk yield and milk composition. Therefore, it can be concluded that supplemental β-carotene does not influence milk yield and milk composition of Saanen goats during the drying off period.

Ensiling characteristics of total mixed ration prepared with local feed resources in Mozambique and their effects on nutrition value and milk production in Jersey dairy cattle.

To effectively utilize total mixed ration (TMR) prepared with locally available feed resources, we studied the nutritional value and milk production in Jersey dairy cattle fed a local general diet (LGD) and fermented TMR in Mozambique. Ten head of Jersey dairy cattle with 337±19.8kg body weight, aged 3-4years in mid location were used in this study. The LGD diet was designed following the general feeding method of local smallholding farms; it contained native grass, Napier grass, wheat bran, and mineral-vitamin mix. Fermented TMR was prepared using Napier grass, corn bran, wheat bran, formula feed, and mineral-vitamin mix. Fermented TMR was preserved as good quality, with a relatively low pH and high lactic acid content. Compared to LGD, fermented TMR significantly improved the dry matter intake and digestibility in dairy cattle. Milk yield was significantly higher in dairy cattle fed with TMR than in those fed with LGD, by 3.75L/d; milk quality was not significantly different between treatments. The results confirmed that LGD had a low dry matter intake and milk yield, and fermented TMR prepared with local feed resources can attain good quality and improve milk yield in dairy cattle in Mozambique.

Milk fatty acid composition, rumen microbial population and animal performance in response to diets rich in linoleic acid supplemented with

Context Rumen biohydrogenation is an important way to produce conjugated linoleic acid (CLA), especially the rumenic acid isomer. However, CLA is principally synthesised endogenously in lactating mammals by delta 9-desaturase in breast tissue. Aims The aim of the study was to evaluate milk fatty acid profile, rumen microbial population and animal performance in response to diets containing sunflower oil either supplemented with or without flavonoids and essential oils from Piper betle L. powder (PP) in dairy goats. Method Twelve multiparous Saanen goats (42 ± 1.00 kg; mean ± s.d.) were randomly assigned to two treatment groups in an experiment that lasted for 6 weeks. The two experimental diets formulated as total mixed ration were: control (CTH) diet (containing 0% PP) and DPB diet (CTH diet containing 1.3% PP on a dry-matter basis). Key results Inclusion of flavonoids and essential oils from PP in the diet (DPB) did not affect dry-matter intake but resulted in a greater milk yield and altered the composition of milk. Compared with the control diet (CTH), the DPB diet decreased the saturated fatty acid concentration and increased the unsaturated fatty acid concentration in milk. Inclusion of PP decreased the C18:0 production (P &lt; 0.05), resulting in higher C18:1 trans11 and C18:2 cis9 trans11 (P &lt; 0.05) concentrations. Overall, DPB diet increased the total CLA by 1.5-fold, from 1.77 to 2.62 g/100 g fatty acid. The desaturase rate (except desaturase for carbon 18, P &lt; 0.05), and atherogenic and thrombogenic indices were not affected by inclusion of PP in the DPB diet. Moreover, the DPB diet escalated total volatile fatty acid production and altered the volatile fatty acid profile. Compared with goats fed with CTH diet, PP supplementation increased the presence of ruminal Butyrivibrio fibrisolvens by ~5-fold, but the presence of B. proteoclasticus decreased to about 1/11 of the control. Conclusions The use of sunflower oil at 17.6 g/kg diet and inclusion of a practical dose of flavonoids and essential oils from Piper betle L. leaves in the diet of dairy goats can be an efficient method to improve milk yield and milk composition, including increasing the CLA concentration of milk. Implications These results constitute an alternative strategy to improve milk quality, without negatively affecting animal performance.

Effect of supplementation with rumen-protected choline and green tea extract on production performance of transition Karan Fries cows.

Aim:The main objective of this study was to estimate the effect of supplementation of rumen-protected choline (RPC) and green tea extract (GTE) on production parameters in transition Karan Fries (KF) cows.Materials and Methods:The present experiment was carried out on 32 pregnant KF cows. In the control group, cows were fed basal diet. In T1, each cow was fed RPC (55 g/day), in T2 – GTE (3 g/d), and in T3– RPC + GTE (55+3) g/day along with basal diet. The duration of the experiment was 30 days before calving to 60 days after parturition.Results:Feeding of both RPC and GTE significantly increased milk yield (p≤0.01), 4% fat corrected milk yield (p≤0.01), milk fat (p≤0.01), and total solid content (p≤0.05) than control. There was no significant difference (p≥0.05) in milk protein, lactose, and solids not fat (SNF) content among the groups.Conclusion:Supplementing RPC and GTE in combination improved milk yield and fat content of the milk without altering protein, lactose content of the milk in transition KF cows.

Reductive acetogens isolated from ruminants and their effect on in vitro methane mitigation and milk performance in Holstein cows.

This study was designed to evaluate the in vitro and in vivo effects of reductive acetogens isolated from ruminants on methane mitigation, and milk performance, respectively. Four acetogens, Proteiniphilum acetatigenes DA02, P. acetatigenes GA01, Alkaliphilus crotonatoxidans GA02, and P. acetatigenes GA03 strains were isolated from ruminants and used in in vitro experiment. A control (without acetogen) and a positive group (with Eubacterium limosum ATCC 8486) were also included in in vitro experiment. Based on higher acetate as well as lower methane producing ability in in vitro trial, P. acetatigenes GA03 was used as inoculum for in vivo experiment. Holstein dairy cows (n = 14) were divided into two groups viz. control (without) and GA03 group (diet supplied with P. acetatigenes GA03 at a feed rate of 1% supplementation). Milk performance and blood parameters were checked for both groups. In in vitro, the total volatile fatty acids and acetate production were higher (p < 0.05) in all 4 isolated acetogens than the control and positive treatment. Also, all acetogens significantly lowered (p < 0.05) methane production in comparison to positive and control groups however, GA03 had the lowest (p < 0.05) methane production among 4 isolates. In in vivo, the rate of milk yield reduction was higher (p < 0.05) in the control than GA03 treated group (5.07 vs 2.4 kg). Similarly, the decrease in milk fat was also higher in control (0.14% vs 0.09%) than treatment. The somatic cell counts (SCC; ×103/mL) was decreased from 128.43 to 107.00 in acetogen treated group however, increased in control from 138.14 to 395.71. In addition, GA03 increased blood glucose and decreased non-esterified fatty acids. Our results suggest that the isolated acetogens have the potential for in vitro methane reduction and P. acetatigenes GA03 strain could be a candidate probiotic strain for improving milk yield and milk fat in lactating cows with lowering SCCs.

Effects of feeding rice gruel based milk replacer on growth, carcass characteristics and rumen development of goat kids

Supplementation of concentrate feed during late pregnancy of doe is an approach to improve milk yield and kid performance after kidding. The experiment was conducted to find out appropriate level and duration of supplementation to the pregnant doe. The data of the experiment were analyzed applying Completely Randomized Design (CRD) with factorial arrangements with three replications. First factor used in the experiment was the duration of concentrate feeding (15 days, 30 days, 45 days and 60 days) and second was amount of concentrate supplemented (2.00%, 1.75%, 1.50% and 1.25% of their body weight). Birth weight, weaning weight, milk yield and kid mortality in different groups were recorded. Birth weight and weaning weight were found higher in 60 days supplemented group i.e. 2.3±0.23 kg and 10.94±0.41 kg, respectively. Both birth weight and weaning weight were also found higher with 2 percent concentrate feeding group than others i.e. 2.1±0.23 kg and 9.64±0.42 kg, respectively. Milk yield recorded for fourth week shows that higher milk yields were found in 2 percent concentrate supplemented group with 60 days time period. The result showed that by increasing the level of concentrate during late gestation period of does increased the birth weight, weaning weight of kids and increased milk yield of does. So the higher level of concentrate supplementation is required during 45 to 60 days of late gestation in does.&#x0D; Bang. J. Anim. Sci. 2019. 48 (1): 57-66