This research aimed to analyze the prevalence of root dilaceration in buccally impacted canines (BICs) and palatally impacted canines (PICs) with their adjacent teeth based on a retrospective cone-beam computed tomography (CBCT) investigation. Pretreatment CBCT images of 145 subjects with unilateral maxillary canine impaction and 145 age- and sex-matched subjects without impaction were used. Prevalence of dilaceration (subclassified to root curvature and apical hook based on severity) in canines and adjacent teeth was determined in CBCT records. The root length of maxillary impacted canines was measured for further morphologic evaluations. Impacted canines had a significantly higher prevalence of root dilaceration than the control group and compared with the erupted contralateral canines in the experimental group (P<0.001 for both). A significantly higher prevalence of root dilaceration was found in adjacent lateral incisors of the PICs subgroup than that of the control group (P<0.001). Adjacent premolars had a higher prevalence of dilacerated roots in the PICs subgroup (P<0.001) than the control group, but not for the BICs subgroup. Significantly higher prevalence of curvature (P<0.001 for both) and hook (P=0.008 and P<0.001, respectively) were found in BICs and PICs roots compared with the control group. Both types of impacted canines had significantly shorter roots than the control group (P<0.001 for both). BICs and PICs have a higher tendency to present root dilaceration and shorter roots. Unlike BICs, adjacent teethto PICs were more frequently observed to have root dilaceration.