Image Plane Research Articles

Orthogonal-view microscope for the biomechanics investigations of aquatic organisms

Microscopes are essential for the biomechanical and hydrodynamical investigation of small aquatic organisms. We report a prototype of a do-it-yourself microscope that enables the visualization of organisms from two orthogonal imaging planes — top and side views. Compared to conventional imaging systems, this approach provides a comprehensive visualization strategy of organisms, which could have complex shapes and morphologies. The microscope was constructed by combining custom 3D-printed parts and off-the-shelf components. The system is designed for modularity and reconfigurability. Open-source design files and build instructions are provided in this report. Additionally, proof-of-use experiments (particularly with Hydra) and other organisms that combine the imaging with an analysis pipeline were demonstrated to highlight the system’s utility. Beyond the applications demonstrated, the system can be used or modified for various imaging applications.

Robust and adaptive star identification algorithm based on linear assignment for multiple large field of view visual imaging systems

The integration of the visual imaging system and the self-attitude determination system in on-orbit space projects necessitates robust star identification algorithms. However, disturbances in the on-orbit environment pose a challenge to the accuracy and efficiency of star identification algorithms. This paper introduces a novel star identification algorithm, to the best of our knowledge, designed for multiple large field of view (FOV) visual imaging systems, providing stability in the presence of the noise types, including position noise, lost-star noise, and fake-star noise. We employ the dynamic simulated star images generation method to construct simulated star image libraries suitable for various cameras with different FOV angles. Our algorithm comprises two parts: the star edge matching for coarse matching of interstellar angular distances based on linear assignment, and the star point registration for precise matching of star vectors. This innovative combination of local edge generation and global matching approach achieves an impressive 97.83% identification accuracy, maintaining this performance even with a standard deviation of one pixel in image plane errors and up to five missing stars. A comprehensive approach involving both simulated and empirical experiments was employed to validate the algorithm’s effectiveness. This integration of the visual imaging system and the self-attitude determination system offers potential benefits such as reduced space equipment weight, simplified satellite launch processes, and decreased maintenance costs.

Laser differential confocal axicon surface profile measurement method

Aiming to address the challenging problem of high-precision axicon profile measurement, a laser differential confocal axicon profile normal tracking (LDCNT) measurement method with high spatial resolution and anti-tilt capability is proposed in this paper. This method utilizes the principle of normal direction adaptive tracking for axicon profile measurements. It employs the Phase-Sensitive Detector (PSD) image plane spot position to calculate the normal angle and a high-precision rotary axis to drive the sensor normal to the measured surface to achieve the normal direction adaptive tracking measurement of the axicon profile and reduce the difficulty of pose adjustment of the measured axicon. Using the laser differential confocal focusing principle, the differential confocal signal is normalized to improve the axial resolution and avoid the Abbe error generated by the local inclination of the surface, thus realizing high-resolution and anti-tilt measurement of the axicon profile. Through the reference frame error decoupling axicon profile measurement and evaluation technique, spatial error compensation, three-dimensional profile reconstruction, and evaluation of the measurement data are performed to reduce the systematic measurement error and realize high-precision measurement and reconstruction of the axicon profile. Finally, the feasibility of the method is verified by measuring the profile of a standard cylinder and axicon at different axicon angles. The experimental analyses show that this method provides a new method for high-precision measurement of the axicon profile with Peak-to-Valley (PV) repeatability better than 40 nm (3σ) and Root-Mean-Square (RMS) repeatability better than 15 nm (3σ).

Quantitative phase imaging with a compact meta-microscope

Quantitative phase imaging (QPI) based on the transport-of-intensity equation (TIE) is a powerful technique in label-free microscopy. The image stack required for a successful TIE-QPI is traditionally obtained by translating the object or image plane, and the optical elements used in the conventional TIE-QPI systems are usually bulky and cumbersome. Stable and compact TIE-QPI methods capable of non-motion optical zooming can significantly facilitate applications that demand portability. Here, we propose a non-motion TIE-QPI method based on a dispersive metalens. The dispersive nature of the metalens is utilized to provide a spectral focal tuning. With fixed object and image planes, seven through-focus intensity images are captured by changing the illumination wavelength. The QPI performance is validated by retrieving the surface phase profiles of a microlens array and a phase resolution target, showing a high phase detection accuracy (deviation less than 0.03 wavelength). Subsequently, we established a compact meta-microscope by integrating the metalens with a commercially available CMOS image sensor, which shows good performance in microscopic imaging of unstained bio-samples. Our approach, based on the large-dispersive metalens, facilitates a compact and robust QPI system for optical metrology and label-free microscopy.

Space‐Time Coding Metasurface For Multifunctional Holographic Imaging

AbstractHolographic imaging as one of promising image techniques reconstructs object image using the amplitude and phase information scattered from objects. Programmable metasurface composed of digitally coding unit cells can arbitrarily manipulate amplitude and phase of the scattering wave by tailoring operation states of the unit cells, and thus achieves high‐resolution imaging reconstruction. However, the conventional metasurface can only achieves a desired holographic image on an imaging plane. This paper presented a time‐space coding 2‐bit metasurface to overcome this challenge. By rapidly switching PIN diodes loaded in metasurface unit cell according to the customized time sequences, the magnitude and phase of the ±1st order harmonic fields can be fully controlled, achieving the hologram imaging at the higher order harmonics. The proof‐of‐concept measurements are given to demonstrate the versatile holographic imaging functions at the ±1st order harmonics including two different holographic images on an imaging plane, holographic imaging and beam scanning, and holographic imaging and orbital angular momentum (OAM) wave generation, which undoubtedly enriches the applications of the programmable metasurfaces in the field of the holographic imaging.

Fundamental Limits on Earth-like Exoplanet Imaging with Large Telescopes Employing Laser Tomographic Adaptive Optics Systems: A Comparative Analysis of LGS AO and LTAO Systems

Exoplanet imaging with high-contrast imaging adaptive optics systems, though challenging, is a promising path toward the characterization of terrestrial planets. We analyzed the fundamental limitations associated with the direct imaging of terrestrial exoplanets around low-mass stars with Extremely Large Telescopes using laser tomographic adaptive optics (LTAO) and derived the post-coronagraph image shape in the focal plane from LTAO systems. Additionally, the fundamental limitation of direct imaging was found to come from unseen spatial frequencies during tomographic reconstruction. Through the provision of optimization strategies for laser guide star (LGS) asterisms, based on the post-coronagraph image contrast, we aimed to assist in the design of LTAO systems for Extremely Large Telescopes, resulting in a six-fold improvement in the LTAO post-coronagraph image plane at 0.1 arcseconds.

Fitting pseudo-Sérsic (Spergel) light profiles to galaxies in interferometric data: The excellence of the uυ-plane

Modern (sub)millimeter interferometers, such as ALMA and NOEMA, offer high angular resolution and unprecedented sensitivity. This provides the possibility to characterize the morphology of the gas and dust in distant galaxies. To assess the capabilities of the current software in recovering morphologies and surface brightness profiles in interferometric observations, we tested the performance of the Spergel model for fitting in the uυ-plane, which has been recently implemented in the IRAM software GILDAS (uv_fit). Spergel profiles provide an alternative to the Sérsic profile, with the advantage of having an analytical Fourier transform, making them ideal for modeling visibilities in the uυ-plane. We provide an approximate conversion between the Spergel index and the Sérsic index, which depends on the ratio of the galaxy size to the angular resolution of the data. We show through extensive simulations that Spergel modeling in the uυ-plane is a more reliable method for parameter estimation than modeling in the image plane, as it returns parameters that are less affected by systematic biases and results in a higher effective signal-to-noise ratio. The better performance in the uυ-plane is likely driven by the difficulty of accounting for a correlated signal in interferometric images. Even in the uυ-plane, the integrated source flux needs to be at least 50 times larger than the noise per beam to enable a reasonably good measurement of a Spergel index. We characterized the performance of Spergel model fitting in detail by showing that parameter biases are generally low (<10%) and that uncertainties returned by uv_fit are reliable within a factor of two. Finally, we showcase the power of Spergel fitting by reexamining two claims of extended halos around galaxies from the literature, showing that galaxies and halos can be successfully fitted simultaneously with a single Spergel model.

Effects of Diameter between Electrodes on Properties of Electrostatic Quadrupole Deflector

Abstract: This study investigates the impact of varying the electrostatic quadrupole deflector diameter (EQDD) on the image when charged particles undergo deflection. A comparison is made between aberration figures, the kinetic energy of the electron beam, and the flight time concerning different EQDD values. The results showed that the most effective image is created at the image plane when the EQDD is double the radius of the electrostatic quadrupole deflector electrode (r). Keywords: Charge particle, Electrostatic quadrupole deflector, SIMION 8.1.

In vivo bistatic dual-aperture ultrasound imaging and elastography of the abdominal aorta.

Introduction: In this paper we introduce in vivo multi-aperture ultrasound imaging and elastography of the abdominal aorta. Monitoring of the geometry and growth of abdominal aortic aneurysms (AAA) is paramount for risk stratification and intervention planning. However, such an assessment is limited by the lateral lumen-wall contrast and resolution of conventional ultrasound. Here, an in vivo dual-aperture bistatic imaging approach is shown to improve abdominal ultrasound and strain imaging quality significantly. By scanning the aorta from different directions, a larger part of the vessel circumference can be visualized. Methods: In this first-in-man volunteer study, the performance of multi-aperture ultrasound imaging and elastography of the abdominal aortic wall was assessed in 20 healthy volunteers. Dual-probe acquisition was performed in which two curved array transducers were aligned in the same imaging plane. The transducers alternately transmit and both probes receive simultaneously on each transmit event, which allows for the reconstruction of four ultrasound signals. Automatic probe localization was achieved by optimizing the coherence of the trans-probe data, using a gradient descent algorithm. Speckle-tracking was performed on the four individual bistatic signals, after which the respective axial displacements were compounded and strains were calculated. Results: Using bistatic multi-aperture ultrasound imaging, the image quality of the ultrasound images, i.e., the angular coverage of the wall, was improved which enables accurate estimation of local motion dynamics and strain in the abdominal aortic wall. The motion tracking error was reduced from 1.3 mm ± 0.63 mm to 0.16 mm ± 0.076 mm, which increased the circumferential elastographic signal-to-noise ratio (SNRe) by 12.3 dB ± 8.3 dB on average, revealing more accurate and homogeneous strain estimates compared to single-perspective ultrasound. Conclusion: Multi-aperture ultrasound imaging and elastography is feasible in vivo and can provide the clinician with vital information about the anatomical and mechanical state of AAAs in the future.

Spacecraft Formation Keeping and Reconfiguration Using Optimal Visual Servoing

This paper proposes a direct visual servoing system for spacecraft guidance in formation flying scenarios. The proposed image-based visual servoing system uses image information for planning and executing formation acquisition, reconfiguration, and maintenance maneuvers. The system assumes that LEDs are located at specific points on the satellites, enabling the visual servoing controller to rely on continuous tracking of these features in the camera’s image plane. Analytical developments demonstrate that the proposed optimal visual control system is stable and optimal, and it acts on both the orbital and attitude dynamics of the spacecraft, considering circular and elliptical reference orbits. The distributed image-based controller defines a cost function that minimizes control efforts, and the paper proses an optimal framework for developing controllers that address the issue. A ROS-based simulation tool was used to test the proposed visual servoing controller in a realistic small-sat formation flying scenario. Results indicate that the proposed distributed control strategy is viable and robust against environmental perturbations and disturbances in sensing and actuation.

UCMCTrack: Multi-Object Tracking with Uniform Camera Motion Compensation

Multi-object tracking (MOT) in video sequences remains a challenging task, especially in scenarios with significant camera movements. This is because targets can drift considerably on the image plane, leading to erroneous tracking outcomes. Addressing such challenges typically requires supplementary appearance cues or Camera Motion Compensation (CMC). While these strategies are effective, they also introduce a considerable computational burden, posing challenges for real-time MOT. In response to this, we introduce UCMCTrack, a novel motion model-based tracker robust to camera movements. Unlike conventional CMC that computes compensation parameters frame-by-frame, UCMCTrack consistently applies the same compensation parameters throughout a video sequence. It employs a Kalman filter on the ground plane and introduces the Mapped Mahalanobis Distance (MMD) as an alternative to the traditional Intersection over Union (IoU) distance measure. By leveraging projected probability distributions on the ground plane, our approach efficiently captures motion patterns and adeptly manages uncertainties introduced by homography projections. Remarkably, UCMCTrack, relying solely on motion cues, achieves state-of-the-art performance across a variety of challenging datasets, including MOT17, MOT20, DanceTrack and KITTI. More details and code are available at https://github.com/corfyi/UCMCTrack.

Deep learning-based automatic pipeline for 3D needle localization on intra-procedural 3D MRI

PurposeAccurate and rapid needle localization on 3D magnetic resonance imaging (MRI) is critical for MRI-guided percutaneous interventions. The current workflow requires manual needle localization on 3D MRI, which is time-consuming and cumbersome. Automatic methods using 2D deep learning networks for needle segmentation require manual image plane localization, while 3D networks are challenged by the need for sufficient training datasets. This work aimed to develop an automatic deep learning-based pipeline for accurate and rapid 3D needle localization on in vivo intra-procedural 3D MRI using a limited training dataset.MethodsThe proposed automatic pipeline adopted Shifted Window (Swin) Transformers and employed a coarse-to-fine segmentation strategy: (1) initial 3D needle feature segmentation with 3D Swin UNEt TRansfomer (UNETR); (2) generation of a 2D reformatted image containing the needle feature; (3) fine 2D needle feature segmentation with 2D Swin Transformer and calculation of 3D needle tip position and axis orientation. Pre-training and data augmentation were performed to improve network training. The pipeline was evaluated via cross-validation with 49 in vivo intra-procedural 3D MR images from preclinical pig experiments. The needle tip and axis localization errors were compared with human intra-reader variation using the Wilcoxon signed rank test, with p < 0.05 considered significant.ResultsThe average end-to-end computational time for the pipeline was 6 s per 3D volume. The median Dice scores of the 3D Swin UNETR and 2D Swin Transformer in the pipeline were 0.80 and 0.93, respectively. The median 3D needle tip and axis localization errors were 1.48 mm (1.09 pixels) and 0.98°, respectively. Needle tip localization errors were significantly smaller than human intra-reader variation (median 1.70 mm; p < 0.01).ConclusionThe proposed automatic pipeline achieved rapid pixel-level 3D needle localization on intra-procedural 3D MRI without requiring a large 3D training dataset and has the potential to assist MRI-guided percutaneous interventions.

Abstract 4919: AI-enabled hit selection of drug screening on human pancreatic cancer organoids

Abstract Cancer remains one of the leading causes of death in the 21st century. Despite the latest advances in oncology, most cancer patients lack tailored therapeutic approaches with lasting benefit. Measuring the impact of anticancer compounds and their combinations is only possible on ex vivo assays. To this end, patient-derived organoids (PDOs) have been proposed as viable and efficient models for ex vivo testing. PDOs show long-term expansion potential while retaining tumor histopathology as well as cancer gene mutations. However, the translation of organoids in screening applications has so far been hampered by the lack of homogeneity and difficulties in handling and automation. Moreover, organoids are typically randomly distributed across the culture which complicates imaging and images analyses. To overcome these challenges, we set up a compound screening workflow with PDOs using Gri3D platform, comprising plates with micro-cavities suitable for high-throughput and reproducible organoid culture. Based on a standard 96 microtiter plate, each well contains a microwell array patterned in a cell repellent hydrogel. On Gri3D®, organoids are robustly generated in the microwells and are located in the same imaging plane. This greatly facilitates quantitative analyses in high content image-based screens. Furthermore, the pipetting port enables automation of cell seeding, media exchange and compound incubation with liquid-handlers, thus increasing assay reproducibility. In the presented work, we exposed human pancreatic cancer PDOs to a panel of anti-cancer compounds at different doses and followed their response to the drugs using viability dyes Calcein AM (live stain) and Ethidium Homodimer-1 (dead stain) with high-content confocal imaging. Using an AI-based approach, we efficiently detected each single organoid and extracted phenotypic features from all three channels (TL, live stain and dead stain) which correlate with cytotoxicity. The extracted features (more than 100) were first dimensionally reduced to 3 components using either PCA or UMAP, the treatments were then visualized in 3D scatter plots, ranked and clustered using machine learning. The data suggests that the compound treatment Palbociclib 50uM has significant cytotoxicity effects similar to the positive control, which is consistent with the traditional live/dead analysis. The AI approach demonstrates feasibility to perform drug screening in a robust and un-biased data analysis approach. Citation Format: Zhisong Tong, Angeline Lim, Marine Meyer, Maria Clapes, Oksana Sirenko, Nathalie Brandenberg. AI-enabled hit selection of drug screening on human pancreatic cancer organoids [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 4919.

Self-supervised learning for medical image data with anatomy-oriented imaging planes

Self-supervised learning has emerged as a powerful tool for pretraining deep networks on unlabeled data, prior to transfer learning of target tasks with limited annotation. The relevance between the pretraining pretext and target tasks is crucial to the success of transfer learning. Various pretext tasks have been proposed to utilize properties of medical image data (e.g., three dimensionality), which are more relevant to medical image analysis than generic ones for natural images. However, previous work rarely paid attention to data with anatomy-oriented imaging planes, e.g., standard cardiac magnetic resonance imaging views. As these imaging planes are defined according to the anatomy of the imaged organ, pretext tasks effectively exploiting this information can pretrain the networks to gain knowledge on the organ of interest. In this work, we propose two complementary pretext tasks for this group of medical image data based on the spatial relationship of the imaging planes. The first is to learn the relative orientation between the imaging planes and implemented as regressing their intersecting lines. The second exploits parallel imaging planes to regress their relative slice locations within a stack. Both pretext tasks are conceptually straightforward and easy to implement, and can be combined in multitask learning for better representation learning. Thorough experiments on two anatomical structures (heart and knee) and representative target tasks (semantic segmentation and classification) demonstrate that the proposed pretext tasks are effective in pretraining deep networks for remarkably boosted performance on the target tasks, and superior to other recent approaches.

A Coupled Calibration Method for Dual Cameras-Projector System with Sub-Pixel Accuracy Feature Extraction.

Binocular structured light systems are widely used in 3D measurements. In the condition of complex and local highly reflective scenes, to obtain more 3D information, binocular systems are usually divided into two pairs of devices, each having a Single Camera and a Projector (SCP). In this case, the binocular system can be seen as Dual Cameras-Projector (DCP) system. In the DCP calibration, the Left-SCP and Right-SCP need to be calibrated separately, which leads to inconsistent parameters for the same projector, thus reducing the measurement accuracy. To solve this problem and improve manoeuvrability, a coupled calibration method using an orthogonal phase target is proposed. The 3D coordinates on a phase target are uniquely determined by the binocular camera in DCP, rather than being calculated separately in each SCP. This ensures the consistency of the projector parameters. The coordinates of the projector image plane are calculated through the unwrapped phase, while the parameters are calibrated by the plane calibration method. In order to extract sub-pixel accuracy feature points, a method based on polynomial fitting using an orthogonal phase target is exploited. The experimental results show that the reprojection error of our method is less than 0.033 pixels, which improves the calibration accuracy.

An 8 mm endoscopic histotripsy array with integrated high-resolution ultrasound imaging

An 8 mm diameter, image-guided, annular array histotripsy transducer was fabricated and characterized. The array was laser etched on a 5 MHz, 1–3 dice and fill, PZT-5H/epoxy composite with a 45 % volume fraction. Flexible PCBs were used to electrically connect to the array elements using wirebonds. The array was backed with a low acoustic impedance epoxy mixture. A 3.6 by 3.8 mm, 64-element, 30 MHz phased array imaging probe was positioned in the center hole, to co-align the imaging plane with the bubble cloud produced by the therapy array. A custom 16-channel high voltage pulse generator was used to test the annular array for focal lengths ranging from 3- to 8-mm. An aluminum lens-focussed transducer with a 7 mm focal length was fabricated using the same piezocomposite and backing material and tested along with the histotripsy array. Simulated results from COMSOL FEM models were compared to measured results for low voltage characterization of the array and lens-focussed transducer. The measured transmit sensitivity of the array ranged from 0.113 to 0.167 MPa/V, while the lens-focussed transducer was 0.192 MPa/V. Simulated values were 0.160 to 0.174 MPa/V and 0.169 MPa/V, respectively. The measured acoustic fields showed a significantly increased depth-of-field compared the lens-focussed transducer, while the beamwidths of the array focus were comparable to the lens. The measured cavitation voltage in water was between 254 V and 498 V depending on the focal length, and 336 V for the lens-focussed transducer. The array had a lower cavitation voltage than the lens-focussed transducer for a comparable operating depth. The histotripsy array was tested in a tissue phantom and an in vivo rat brain. It was used to produce an elongated lesion in the brain by electronically steering the focal length from 3- to 8-mm axially. Real time ultrasound imaging with a Doppler overlay was used to target the tissue and monitor ablation progress, and histology confirmed the targeted tissue was fully homogenized.

Two-dimensional binary phase gratings for zero-order and high-order diffraction suppression.

A two-dimensional binary phase grating is proposed in this paper. Unlike a conventional transmission grating, in theory, the proposed phase grating can simultaneously eliminate the zero- and high-order diffraction along certain axes on the image plane, forming a pure sinusoidal transmission modulation that leaves only the first-order diffraction. The first-ever, to the best of our knowledge, theoretical model for achieving sinusoidal transmission modulation is suggested in this paper; then the theoretical calculation and experiment results are displayed to investigate the physical mechanism of the proposed grating. Moreover, the manipulation on the arrangement of grating design can disperse or concentrate the diffraction energy at a specific axis. Finally, almost first-order-only diffraction is achieved on a single axis by introducing random changes to certain geometrical parameters of the two-dimensional binary phase grating. Our work provides potential applications in optical science and engineering fields.

Expanding Sparse Radar Depth Based on Joint Bilateral Filter for Radar-Guided Monocular Depth Estimation.

Radar data can provide additional depth information for monocular depth estimation. It provides a cost-effective solution and is robust in various weather conditions, particularly when compared with lidar. Given the sparse and limited vertical field of view of radar signals, existing methods employ either a vertical extension of radar points or the training of a preprocessing neural network to extend sparse radar points under lidar supervision. In this work, we present a novel radar expansion technique inspired by the joint bilateral filter, tailored for radar-guided monocular depth estimation. Our approach is motivated by the synergy of spatial and range kernels within the joint bilateral filter. Unlike traditional methods that assign a weighted average of nearby pixels to the current pixel, we expand sparse radar points by calculating a confidence score based on the values of spatial and range kernels. Additionally, we propose the use of a range-aware window size for radar expansion instead of a fixed window size in the image plane. Our proposed method effectively increases the number of radar points from an average of 39 points in a raw radar frame to an average of 100 K points. Notably, the expanded radar exhibits fewer intrinsic errors when compared with raw radar and previous methodologies. To validate our approach, we assess our proposed depth estimation model on the nuScenes dataset. Comparative evaluations with existing radar-guided depth estimation models demonstrate its state-of-the-art performance.

Optimal Space-Borne ISAR Imaging of Space Objects with Co-Maximization of Doppler Spread and Spacecraft Component Area

The Inverse Synthetic Aperture Radar (ISAR) has been proven to be an effective tool for space target sensing due to its capability of performing high-resolution imaging. Since the component information of the spacecraft is key to the identification of the target and diagnosis of its status, ISAR images with a clear and complete representation of the typical components are much desired. This requires a selection of the imaging time, during which a certain spacecraft component has a good projection on the ISAR image plane with the shape feature well conserved and a high resolution. In addition, a fully automated implementation with a high computational efficiency is also highly preferred for on-orbit operations so as to improve the intelligence level of the space-borne system. We propose a bicriterion-based automated optimal imaging time-selection method for the space-borne ISAR, which is seeking the slow time section of the data that result in the best image. A good image means a high azimuth resolution and the best presentation of the solar panels. One criterion is the Maximum Doppler Spread (MDS), which indicates the maximum Effective Rotational Velocity (ERV) leading to high image resolution, but it is influenced by the satellite attitude. of the spacecraft. The other is the Maximum Component Area (MCA), which is defined to indicate the completeness of the component considered. The radar echoes are processed sequentially by way of a sliding window. The interval with the co-maximization of the DS and CA is selected, and fine processing is performed further to obtain the best images. The results of the simulation experiments show that the proposed method can achieve spacecraft images with the solar panels presented the best. The computational complexity is low.

Characterization of real-time cine MR imaging distortion on 0.35 T MRgRT with concentric cine imaging QA phantom

Objective. Real-time MRgRT uses 2D-cine imaging for target tracking and motion evaluation. Rotation of gantry induced B 0 off-resonance, resulting in image artifacts and imaging isocenter-shift precluding MR-guided arc therapy. Standard MRI phantoms designed for higher resolution images face challenges when low-resolution cine imaging is needed to achieve high frame rates. This work aimed to examine the spatial accuracy including geometric distortion and isocenter shift in real-time during gantry rotation on a 0.35 T MR-Linac using the concentric Cine imaging quality assurance (QA) phantom and its associated image analysis software. Approach. The Cine imaging QA phantom consists of two concentric shells of low-T1 mineral oil and a central alignment structure. The phantom was scanned on three different MRI systems; 0.55 T Siemens Free.Max, 1.5 T Philips Ingenia, and 0.35 T ViewRay MRIdian MR-Linac using 2D balanced steady-state free precession (bSSFP) imaging sequence. In addition, bSSFP cine MRI with the banding artifact correction was tested on 0.35 T ViewRay MR-Linac. Images from the MR-Linac were acquired with the Linac gantry stationary and rotating from gantry 300°→ 0° and vice versa. Three orthogonal image planes were scanned excluding the 1.5 T Philips Ingenia, where only the axial plane was scanned. The image analysis software calculated the distortion values as well as the isocenter position for each cine frame. Main results. The geometric distortion of cine imaging on MRIs and MR-Linac at gantry stationary are within 1 mm while the substantial geometric distortion of 2 and 2.2 mm were observed on 0.35 T MR-Linac while rotating the gantry clockwise (300°→ 0°) and counterclockwise 0°→ 300° respectively. The average imaging isocenter shift was 0.1 mm for both MRIs and the static gantry and imaging isocenter shift of ≤1.5 mm was observed during the gantry rotation. The imaging isocenter shift decreased by 1 ± 0.2 mm clockwise and counterclockwise with B 0 compensation. Significance. The concentric Cine imaging QA phantom and its associated software effectively demonstrate the image distortion on real-time cine imaging on regular MRIs and 0.35 T MR-Linac. The results of significant geometric distortion with a rotating gantry in the MR-Linac system require further investigation to alleviate the extent of the image distortion.