Aims: Although immediate restoration is recommended after endodontic treatment, delayed restoration is still required in some cases. Also, the presence of the residual endodontic sealer in the pulp chamber may interfere with the adhesive materials and jeopardize the sealability. The purpose of the study was to determine the effect of different sealer residue cleaning methods on dentin bond strength of immediate or delayed endodontic composite restoration. Methods: The occlusal surface of seven freshly extracted human molar teeth without caries were prepared to expose the flat dentin surface. Teeth were randomly divided into six experimental groups according to the cleaning method and restoration timing. Control group was not contaminated with the endodontic sealer and immediately restored (n=10). Cleaning protocols were cotton pellet soaked in saline (CPS) or cotton pellet soaked in 95% ethanol (CPE) or microbrush soaked in 95% ethanol (MBE). After removing of the sealer, a self-etching adhesive system was applied immediately (i) or delayed (d). The microtensile test applied and data were analyzed by Levene's, two way ANOVA and Tukey HSD statistical tests. Results There was no significant difference between the control group and the MBEd and CPSd groups (p>0.05). The bond strength of the CPSi, CPEi, CPEd, MBEi groups was statistically different from control (p<0.05) and there was no significant difference between these groups (p>0.05). Conclusion: CPSd and the MBEd groups showed better microtensile bond strength values than other experimental groups.