Human Body Research Articles

Insights into the Sex-Related Effects of Dietary Polyphenols and Metabolic Disruptors on Inflammatory and (Neuro) Endocrine Pathways in Obesity: The HEAL Project

Background: this study was performed under the umbrella of the Health Extended Alliance for Innovative Therapies, Advanced Lab Research, and Integrated Approaches of Precision Medicine (HEAL ITALIA) partnership and funded under the National Recovery and Resilience Plan, Mission 4 Component 2 Investment 1.3, and by the European Union. Objectives: the overall objective of the HEAL project is to identify innovative and effective therapeutic approaches to reduce disease burden. The present research falls within Spoke 7: Prevention Strategies: Integrated and gender medicine approaches for prevention strategies based on environmental, lifestyle, and clinical biometric data. Obesity represents a primary risk factor worldwide for the onset of numerous life-threatening diseases, including metabolic, cardiovascular, and neurological diseases. Environmental and gender-related factors influence obesity development. However, the molecular mechanisms underlying the effects of those agents on different organs of the human body are not fully understood yet. Methods: here, we present a study protocol aimed at shedding light on (i) the complex interplays among adipose tissue, brain and gut in obesity, and (ii) the effects of specific dietary components and environmental metabolism-disrupting compounds on those interactions. To this purpose, we combined ex vivo, in vitro, and in vivo approaches to gain additional knowledge about the molecular mechanisms underlying connections between organs. Conclusions: the data provided by this study will contribute to defining new targets for therapeutic and/or preventive interventions, thereby allowing more personalized approaches to nutrition.

Does Hand-Predominance Have a Predominant Influence on Craniofacial Asymmetric and Anthropometric Analysis in Preadolescences?

Background: Although the human body generally exhibits bilateral symmetry, achieving perfect symmetry is exceedingly uncommon. During preadolescent development, a face that approximates symmetry is considered both aesthetically and functionally ideal. This study aimed to investigate the relationship between craniofacial traits and hand predominance, using three-dimensional (3D) stereophotogrammetry to discern whether facial soft-tissue characteristics are correlated with hand preference. Materials and Methods: The study involved children aged 9 and 10 years who were free from any diagnosed craniofacial anomalies. Three-dimensional stereophotogrammetry was conducted to analyze their facial morphology, and 37 distinct anatomical landmarks were manually identified using a MATLAB-developed program. Results: A total of 188 Taiwanese children participated in the study. All participants were healthy, with a mean age of 9.79 years. Among them, 93.1% (175) were right-hand predominant, and 6.9% (13) were left-hand predominant. There were no significant differences in linear parameters or facial asymmetry between right-hand-predominant and left-hand-predominant participants (p > 0.05). However, a consistent trend toward right laterality, especially in the right lateral frontal region of the cranium, was observed, as illustrated by heat maps of the average three-dimensional model. Conclusions: The study found no association between facial morphology and hand predominance. A normal asymmetry with a rightward tendency was noted in children aged 9 to 10 years, which was particularly notable in the lateral frontal region of the head.

Sizeism Among Fat and Big Men: A Phenomenological Study

The purpose of this phenomenological study was to capture the lived experiences of sizeism, or size discrimination, for fat and big men. The intention of this study was to identify the essence of fat and big men’s experience of sizeism to more adequately understand how sizeism may impact the lives of these men to assist with the creation of more culturally competent psychological services and interventions for this population. A total of 10 participants were included in this study. Participants self-identified as fat or big, cisgender, and varied in race/ethnic identity and age (from 25 to 44 years). Data analysis of their experiences revealed five emergent themes: (a) accessing masculinity, (b) navigating thin privilege, (c) psychological consequences of living in fat male bodies, (d) sexual capital, (e) and fat/big as identity.

A systematic review and quality assessment of estimated daily intake of microplastics through food.

Plastic waste enters the oceans and soil and is consumed by organisms and humans. Some of the ingested microplastics may remain in the human body and cause toxicity. We conducted a systematic review to estimate the extent to which humans are exposed to microplastics through consumption and performed a quality assessment of research results. We searched for studies published up to December 2023 and included studies that reported on the characteristics and estimated intake of microplastics. The quality assessment tool reported in previous studies was used for food and drinking water studies. We included 76 studies in the analysis, and the types of foods were classified into seven categories: seafood, drinking water, table salt, fruits and vegetables, beverages, condiments, and meat. Theestimated daily intake of microplastics via food was 0.0002-1,531,524 MP/day, with the highest value in bottled water. The quality of food and drinking water studies was evaluated using a quantitative tool to assess reliability. The quality of food studies was 11.50 out of 20 points and the quality of drinking water studies was 11.16 out of 19 points. These results indicate that the closer the score is to the maximum, the more reliable the research findings. The quantitative assessment can be used as an indicator for evaluating the risks of microplastics and can help reduce biases that may occur during the research process. This study confirmed microplastics in foods and human exposure to up to one million microplastics daily. Our study emphasizes the potential for microplastic exposure through food intake and subsequent accumulation in the human body; therefore, efforts are needed to reduce exposure to microplastics in daily life.

Investigation of FF-MAS oxysterole’s role in follicular development and its relation to hedgehog signal pathway

The Hedgehog signaling pathway plays a crucial role in folliculogenesis; however, the association between FF-MAS oxysterol activity in folliculogenesis and the Hedgehog signaling pathway has not been revealed. The evaluation of FF-MAS activity in polycystic ovary syndrome (PCOS) with folliculogenesis disorder might provide a new approach to tackle follicular and oocyte maturation failure. The question is: does FF-MAS oxysterol affect granulosa cell (GC) proliferation? If so, is this effect facilitated through the Hedgehog pathway? To answer these questions, GCs were isolated from follicle fluids obtained from patients undergoing oocyte retrieval during in vitro fertilization (IVF) treatment. After the isolated GCs were incubated in different cell culture media, the levels of Hedgehog pathway components (SMO, Gli1) were measured by using immunohistochemical methods, cytoELISA, and qRT-PCR. Meanwhile, cell proliferation rates were determined. Significant increases (p < 0.001) in SMO and Gli1 expressions and cell proliferation were observed in the FF-MAS-treated subgroups of both PCOS and male factor participants compared to the FF-MAS deficient subgroup. Remarkably, FF-MAS positively affected the pathway components despite the pathway inhibitor cyclopamine. Although the increase in Hedgehog pathway components was slightly higher in the male factor group (MF), it was not statistically significant. In our study, we demonstrated for the first time the molecular effect of FF-MAS on human GCs in folliculogenesis. Since FF-MAS is already used in assisted reproductive techniques in animals and is known to be synthesized in the human body, it could be considered a new approach in human IVF treatments.

Supporting Financial Neutrality in Donation of Organs, Cells, and Tissues.

The avoidance of financial gain in the human body is an international ethical standard that underpins efforts to promote equity in donation and transplantation and to avoid the exploitation of vulnerable populations. The avoidance of financial loss due to donation of organs, tissues, and cells is also now recognized as an ethical imperative that fosters equity in donation and transplantation and supports the well-being of donors and their families. Nevertheless, there has been little progress in achieving financial neutrality in donations in most countries. We present here the findings of an international ethics working group convened in preparation for the 2023 Global Summit on Convergence in Transplantation, held in Santander, Spain, which was tasked with formulating recommendations for action to promote financial neutrality in donation. In particular, we discuss the potential difficulty of distinguishing interventions that address donation-related costs from those that may act as a financial incentive for donation, which may inhibit efforts to cover costs. We also outline some practical strategies to assist governments in designing, implementing, and evaluating policies and programs to support progress toward financial neutrality in donation.

Hydrogel Strategies for Female Reproduction Dysfunction.

Infertility is an important issue for human reproductive health, with over half of all cases of infertility associated with female factors. Dysfunction of the complex female reproductive system may cause infertility. In clinical practice, female infertility is often treated with oral medications and/or surgical procedures, and ultimately with assisted reproductive technologies. Owing to their excellent biocompatibility, low immunogenicity, and adjustable mechanical properties, hydrogels are emerging as valuable tools in the reconstruction of organ function, supplemented by tissue engineering techniques to increase their structure and functionality. Hydrogel-based female reproductive reconstruction strategies targeting the pathological mechanisms of female infertility may provide alternatives for the treatment of ovarian, endometrium/uterine, and fallopian tube dysfunction. In this review, we provide a general introduction to the basic physiology and pathology of the female reproductive system, the limitations of current infertility treatments, and the lack of translation from animal models to human reproductive physiology. We further provide an overview of the current and future potential applications of hydrogels in the treatment of female reproductive system dysfunction, highlighting the great prospects of hydrogel-based strategies in the field of translational medicine, along with the significant challenges to be overcome.

One-Dimensional Flexible Capacitive Sensor with Large Strain and High Stability for Human Motion Monitoring.

Flexible capacitive sensors have attracted the attention of researchers owing to their simple structure, ease of realization, and wearability. Currently, flexible capacitive sensors mainly have three-dimensional and two-dimensional structures, which are subject to several limitations in their applications. A low-cost, high-efficiency, and continuously processable process was used to wrap nylon DTY (PA) filaments on the surface of silver-coated nylon (SCN) core yarns and impregnate them with waterborne polyurethane (WPU) to obtain SCN/PA/WPU composite yarns, which were then utilized in the design of SCN/PA/WPU for the preparation of one-dimensionally structured flexible capacitive sensors. The morphology and mechanical properties of the SCN core yarn, SCN/PA wrapped yarn, and SCN/PA/WPU composite yarn were characterized. The strain-sensing performance of the sensor was analyzed, and the sensor was used to monitor human physiological activities. The sensor exhibited excellent strain capacitance sensing performance with a strain range of up to 140%. With a gauge factor of 0.66 at 10% tensile strain, it can detect strains as low as 1% and has good repeatability, withstanding more than 3200 tensile-unload cycles at 80% strain. The one-dimensional structure sensor can be used to monitor the large-scale movements of joints and muscles in various parts of the human body and the physiological signals of tiny human movements, such as breathing, coughing, and facial expressions, which have potential applications in the fields of sports monitoring and smart wearable.

Effects of Channelling a Peripherally Inserted Central Venous Catheter on Blood Flow

A catheter is a device that is inserted into the venous system to infuse treatment with controlled doses per unit of time. The study of its interaction with blood flow cannot be easily analysed with common analytical methods or different visualization techniques in real life. Computational Fluid Dynamics has become a very useful tool in a wide variety of fields of scientific study and has allowed access to the understanding of the anatomical and physiological functioning of the human body. In this work, Computational Fluid Dynamics is used to study the effects of inserting a catheter on blood flow and the quality of the mixture of blood with the various substances infused through this device. Results show that the insertion of the catheter not only does not worsen the blood circulation but improves it by reducing stagnant zones. Regarding mixture, a homogenization of the fluids in the venous area before their entrance to the heart was observed. Highest quality mixtures correspond to fewer infused fluids and at lower velocity.

The relationship between iron deficiency and allergic diseases

One of the most important trace elements of the human body is iron. This trace element is the most common on earth, with about a third of the world’s population suffering from iron deficiency. According to WHO, the incidence of iron deficiency in the population can range from moderate - 5-19.9%, to high-more than 40%. The main causes of iron deficiency are: insufficient intake from food, impaired absorption or pathological losses. Allergic diseases can cause the formation of iron deficiency as a result of all of the above causes. At the moment, the relationship between iron deficiency and the development of allergic reactions is being investigated. It is known that functional iron deficiency contributes to the development of allergies and exacerbates the course of symptoms in people with allergies. In this article, we summarize the data on the effect of iron deficiency on the immune processes of the human body and predisposition to the formation of atopy.

Rola mikrobioty jelit i jej metabolitów w przebiegu otyłości i cukrzycy

The gut microbiota is an indispensable component of the proper functioning of the human body, as gut microorganisms and their metabolites strongly influence the host's metabolism and immune functions. They also contribute to the biosynthesis of vitamins, production of gut hormones, maintenance of intestinal barrier integrity, protection against pathogens, as well as digestion and absorption of nutrients. Increasingly, the relationship between disturbances in the composition of gut microbiota and the onset of metabolic diseases such as obesity and type 2 diabetes is being emphasized. Understanding the significance of the microbiota in the course of these diseases, its composition, and activity may offer new approaches to their treatment. Increasing attention is being paid to individual species of gut bacteria, among which Akkermansia muciniphila holds a special position, as a decrease in the abundance of this commensal bacterium in the gut is associated with many diseases, including obesity and diabetes.

A triple-mode strategy combining low-temperature photothermal, photodynamic, and chemodynamic therapies for treating infectious skin wounds.

The skin is the first natural barrier of the human body. Bacterial infections severely hinder the healing process of skin wounds and pose a great threat to human health. Therefore, it is particularly urgent to develop new antimicrobial strategies for bacterial pathogen clearance and wound healing. In this study, a metal-organic framework (MOF), Fe-MIL88B-NH2, was incorporated with the photosensitizer indocyanine green (ICG) to construct composite nanoparticles (MOF@ICG NPs) with multiple antibacterial activities. Under mild near-infrared (NIR) irradiation, the photosensitizer ICG in the MOF@ICG NPs undergoes photothermal conversion (∼45 °C) and photodynamic reactions to generate heat and singlet oxygen (1O2). In addition, the Fenton reaction of the NPs with hydrogen peroxide (H2O2) in the bacterial infection microenvironment resulted in the generation of hydroxyl radicals (˙OH), thus achieving the three-mode combination of low-temperature photothermal therapy (PTT)/photodynamic therapy (PDT)/chemodynamic therapy (CDT). The in vitro experimental results showed that MOF@ICG MPs had excellent antibacterial properties and good cytocompatibility, with some ability to promote the migration of L-929 fibroblasts. Furthermore, under NIR irradiation, MOF@ICG NPs could significantly kill bacteria and promote skin wound healing according to the results of animal experiments. The wound healing rate reached 87.1% after 7 days of treatment. The research results break through the limitations of single-mode antibacterial technology and provide certain theoretical guidance and technical support for the research and development of new antibacterial materials.

Enhanced Experimental Setup and Methodology for the Investigation of Corrosion Fatigue in Metallic Biodegradable Implant Materials

Biodegradable implants as bone fixations may present a safe alternative to traditional permanent implants, reducing the risk of infections, promoting bone healing, and eliminating the need for removal surgeries. Structural integrity is an important consideration when choosing an implant material. As a biodegradable implant is being resorbed, until the natural bone has regrown, the implant material needs to provide mechanical stability. However, the corrosive environment of the human body may affect the fatigue life of the material. Conversely, mechanical stress can have an effect on electrochemical corrosion processes. This is known as corrosion fatigue. In the presented work, an experimental setup and methodology was established to analyze the corrosion fatigue of experimental bioresorbable materials while simultaneously monitoring the electrochemical processes. A double-walled measurement cell was constructed for a three-point bending test in Dulbecco‘s Phosphate-Buffered Saline (DPBS− −), which was used as simulated body fluid (SBF), at 37 ± 1 °C. The setup was combined with a three-electrode setup for corrosion measurements. Rod-shaped zinc samples were used to validate the setup’s functionality. Preliminary static and dynamic bending tests were carried out as per the outlined methodology to determine the test parameters. Open-circuit as well as potentiostatic polarization measurements were performed with and without mechanical loading. For the control, fatigue tests were performed in an air environment. The tested zinc samples were inspected via scanning electron microscopy (SEM). Based on the measured mechanical and electrochemical values as well as the SEM images, the effects of the different environments were investigated, and the setup’s functionality was verified. An analysis of the data showed that a comprehensive investigation of corrosion fatigue characteristics is feasible with the outlined approach. Therefore, this novel methodology shows great potential for furthering our understanding of the effects of corrosion on the fatigue of biodegradable implant materials.

Towards a gerontology of everything: A more-than-human perspective

Engaging with the special issue's theme of ‘The growing older of humans, non-humans and more-than-humans’, this commentary provides an overview of how social theory has engaged with the ageing body. Beginning with discussion of initial scholarship in the sociology of the body, the commentary provides thoughts on how more-than-human theory, both ‘old’ and ‘new’ materialisms, can contribute to a deeper understanding of how human bodies age alongside nonhuman living things, situated in place and space. This approach acknowledges the distributed forces, agencies and capacities that are generated with and through the relational encounters of humans with nonhuman agents as they move through the life course. The commentary ends with some suggestions for incorporating a ‘gerontology of everything’ perspective into ageing studies as a step towards more-than-human mutual flourishing.

A GCN and Transformer complementary network for skeleton-based action recognition

Graph Convolution Networks (GCNs) have been widely used in skeleton-based action recognition. Although there are significant progress, the inherent limitation still lies in the restricted receptive field of GCN, hindering its ability to extract global dependencies effectively. And the joints that are structurally separated can also have strong correlation. Previous works rarely explore local and global correlations of joints, leading to insufficiently model the complex dynamics of skeleton sequences. To address this issue, we propose a GCN and Transformer complementary network (GTC-Net) that allows parallel communications between GCN and Transformer domains. Specifically, we introduce a graph convolution and self-attention combined module (GAM), which can effectively leverage the complementarity of GCN and self-attention to perceive local and global dependencies of joints for the human body. Furthermore, in order to address the problems of long-term sequence ordering and position detection, we design a position-aware module (PAM), which can explicitly capture the ordering information and unique identity information for body joints of skeleton sequence. Extensive experiments on NTU RGB+D 60 and NTU RGB+D 120 datasets are conducted to evaluate our proposed method. The results demonstrate that our method can achieve competitive results on both datasets.

Home-based guidance training system with interactive visual feedback using kinect on stroke survivors with moderate to severe motor impairment

The home-based training approach benefits stroke survivors by providing them with an increased amount of training time and greater feasibility in terms of their training schedule, particularly for those with severe motor impairment. Computer-guided training systems provide visual feedback with correct movement patterns during home-based training. This study aimed to investigate the improvement in motor performance among stroke survivors with moderate to severe motor impairment after 800 min of training using a home-based guidance training system with interactive visual feedback. Twelve patients with moderate to severe stroke underwent home-based training, totaling 800 min (20–40 min per session, with a frequency of 3 sessions per week). The home-based guidance training system uses Kinect to reconstruct the 3D human body skeletal model and provides real-time motor feedback during training. The training exercises consisted of six core exercises and eleven optional exercises, including joint exercises, balance control, and coordination. Pre-training and post-training assessments were conducted using the Fugl-Meyer Assessment-Upper Limb (FMA-UE), Fugl-Meyer Assessment-Lower Limb (FMA-LE), Functional Ambulation Categories (FAC), Berg Balance Scale (BBS), Barthel Index (BI), Modified Ashworth Scale (MAS), as well as kinematic data of joint angles and center of mass (COM). The results indicated that motor training led to the attainment of the upper limit of functional range of motion (FROM) in hip abduction, shoulder flexion, and shoulder abduction. However, there was no improvement in the active range of motion (AROM) in the upper extremity (U/E) and lower extremity (L/E) joints, reaching the level of the older healthy population. Significant improvements were observed in both left/right and superior/inferior displacements, as well as body sway in the mediolateral axis of the COM, after 800 min of training. In conclusion, the home-based guidance system using Kinect aids in improving joint kinematics performance at the level of FROM and balance control, accompanied by increased mediolateral body sway of the COM for stroke survivors with moderate to severe stroke. Additionally, spasticity was reduced in both the upper and lower extremities after 800 min of home-based training.

The Impact of Body Odors on Sexual Attraction and Partner Selection: A Review of Cisgender Homosexual Men and Women.

This study examined the role of odors in sexual attraction and partner selection among non-heterosexual individuals. We searched using MESH terms in databases including PubMed, Embase, Scopus, Web of Science, Cochrane Central Register of Controlled Trials, CINAHL, and Clinical Trials. We focused on experimental or quasi-experimental studies where olfactory cues were used in partner selection. The studies had to identify participants' sexual orientation and include heterosexuals for comparison. Seven studies, published between 2005 and 2014, involving 345 participants (Mean age, 27.09years), met the criteria. Results suggest that sexual orientation affects both preference for and production of human body odors and that human body odors appear to provide cues about the sex and sexual orientation of potential partners. Most participants were male, limiting conclusions about the role of pheromones in attraction among non-heterosexual women.

Design and Investigation of a Novel Photonic Crystal Fiber Sensor for Detecting Human Body Protein: Amino Acids of Different Types

Design and Investigation of a Novel Photonic Crystal Fiber Sensor for Detecting Human Body Protein: Amino Acids of Different Types

“The doctors guide to the correct examination of dead human bodies...” by I.V. Buyalsky (1824): the first original Russian guide to forensic medical examination

The year 2024 marks the 235th anniversary of the birth of Ilya Vasilyevich Buyalsky, an outstanding Russian surgeon, anatomist, and forensic physician and Honored Professor of the Imperial Medical and Surgical Academy and academician of the Imperial Academy of Arts. Another anniversary date of this year is associated with Buyalsky’s name: exactly 200 years ago, “The doctors guide to the correct examination of dead human bodies for finding the cause of death, especially in forensic cases,” which was the first original domestic manual on forensic medical examination, was published in the Military Medical Journal. This article comprehensively examined the history of the creation of this work, its main points, and its significance for subsequent generations of Russian forensic medical experts. The article provides information about the activities of Buyalsky in the field of forensic medical examination. An analysis of his work “The doctors guide to the correct examination of dead human bodies...” is presented. The present study shows that Buyalsky was one of the founders of forensic thanatology in our country, and the main provisions of “The doctors guide to the correct examination of dead human bodies...” remain relevant to date.

Condition‐Dependent Female Aggression and Its Effects on Mating Success and Sexual Cannibalism in Jumping Spiders

ABSTRACTPre‐copulatory sexual cannibalism is an extreme form of aggression toward potential mates. In this study, we examined condition‐dependent female aggression and its effects on mating success and frequency of pre‐copulatory sexual cannibalism in the jumping spider Phidippus audax Hentz 1845. We examined two of the leading hypotheses of why sexual cannibalism may occur, (1) female mate choice and (2) adaptive female foraging. We separated 137 adult virgin female P. audax into three feeding treatments: (1) well‐fed spiders, (2) 14‐day food deprived, and (3) 28‐day food deprived. We recorded weight loss (14‐ and 28‐day treatments) and survival rates. We conducted mating trials to measure the proportion of non‐aggressive and aggressive male and female behaviors, male mating success, and pre‐copulatory sexual cannibalism. Females deprived of food had higher weight loss and lower survival rates than well‐fed females. In addition, food deprived female spiders exhibited higher proportions of aggressive behaviors and pre‐copulatory sexual cannibalism, and lower mating success compared to well‐fed spiders. We found that as male size increased females were less likely to cannibalize males, but we found no effect of male body condition on whether a male mated with or was cannibalized by a female. Our results are consistent with the adaptive foraging hypothesis and corroborates prior research on pre‐copulatory sexual cannibalism.