SummaryAn isolated 24-hr perfusion of 12 dog kidneys in Belzer apparatus was performed, and the activities of LDH, ICDH, and β-glucuronidase, LDH isoenzymes pattern, and zinc content in the perfusion fluid were determined. LDH activity in plasma perfusate rises with time of perfusion at two statistically different rates, slowly up to 12 hr, then faster thereafter. Significant correlation exists between LDH, ICDH, and zinc values during perfusion. β-Glucuronidase activity changed only slightly, mostly after 18 hr of perfusion. The magnitude of LDH, ICDH activity, and zinc content corresponds with the function of the kidney post-reimplantation and, in satisfactory 24-hr perfusions, should not exceed 500 U/ml and 200 μg% of Zn, respectively. With time of perfusion, there is a continuous shift in LDH isoenzymes to anaerobic forms (LDH-4 and LDH-5), indicating a fast development and a continuous progression of hy-poxia in the kidney tissue.
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