An experimental study using tissue culture medium as preservation and perfusion fluid

Abstract

A commercially available tissue culture medium has been proven capable of preserving dog kidney function for at least 24 hr after simple cooling. The advantages of using tissue culture medium as preservation fluid instead of plasma or albumin solutions from the infectious and immunological points of view are obvious. An in vitro study was completed using the tissue 1 Code (animal No.) Perfusion time (br) Perfusion pressure mm/Hg Flow ml/min Weight gain pH pO 2 mm/Hg Histological appearance 1 24 70-60 systolic 96 35 7.3 150–180 Grossly normal 2 24 45-40 diastolic 108 30 3 24 96 30 4 48 70-60 systolic 80 35 7.3 150–180 Grossly normal 5 48 45-40 diastolic 120 40 7.4 6 48 100 40 7 72 70-60 systolic 115 40 7.4 150–180 Slight vacuolization of the tubular cells 8 72 45-40 diastolic 96 40 9 72 80 40 10 24 70-60 systolic 110 35 7.3 150–180 Used for transplantation 11 24 45-40 diastolic 120 35 12 24 140 40 13 24 100 30 14 24 96 30 culture medium as perfusion fluid. The results proved that it was possible to perfuse dog kidneys up to 72 hr with stable flow, pressure, pO 2 and pH without adding O 2 or CO 2. In vivo studies, however, showed that the medium was inferior to other perfusion fluids. It was neither possible to restore kidney function after 24 hr perfusion nor after simple cooling for 48 hr due to depositions of fibrin in the glomerular vessels, most probably caused by damage to the endothelial linings.