Horse Oil Research Articles

Characteristics of Purified Horse Oil by Supercritical Fluid Extraction with Different Deodorants Agents.

This study investigated the impact of activated carbon, palm activated carbon, and zeolite on horse oil (HO) extracted from horse neck fat using supercritical fluid extraction with deodorant-untreated HO (CON) as a comparison. The yield and lipid oxidation of deodorant untreated HO (CON) were not significantly affected by the three deodorants. However, deodorant-treated HOs exhibited significantly elevated levels of α-linolenic acid (C18:3n3) and eicosenoic acid (C20:1n9) compared to CON (p<0.05), while other fatty acids remained consistent. Zeolite-purified HO demonstrated significantly lower levels of volatile organic compounds (VOCs) than other treatments (p<0.05). Remarkably, zeolite decreased the concentration of pentane, 2,3-dimethyl (gasoline odor), by over 90%, from 177.17 A.U. ×106 in CON to 15.91 A.U. ×106. Zeolite also effectively eliminates sec-butylamine (ammonia and fishy odor) as compared to other deodorant-treated HOs (p<0.05). Additionally, zeolite reduced VOCs associated with the fruity citrus flavor, such as nonanal, octanal, and D-limonene in HO (p<0.05). This study suggests that integrating zeolite in supercritical fluid extraction enhances HO purification by effectively eliminating undesirable VOCs, presenting a valuable approach for producing high-quality HO production in the cosmetic and functional food industries.

Characterizing the Volatile Profile and Flavor of Four Kinds of Hotpot Seasonings in China Using HS‐GC‐MS, HS‐GC‐IMS, and Sensory Evaluation

AbstractHotpot is a distinctive dish of East Asian cuisine, especially in China. Herein, headspace‐gas chromatography‐mass spectrometry, headspace‐gas chromatography‐ion mobility spectrometry, and sensory evaluation are applied to compare the volatile organic compounds (VOCs) of four types of hotpot seasoning oils (HSOs) and establish their characteristic fingerprints. A total of 203 VOCs are detected by gas chromatography‐mass spectrometry (GC‐MS). Among them, linalool, anethole, and estragole contribute to the overall aroma of all four HSOs (relative odor activity value ≥ 0.1). Gas chromatography‐ion mobility spectrometry (GC‐IMS) results reveal that 77 VOCs are identified, comprising 19 terpenoids, 18 aldehydes, 14 alcohols, 13 esters, 6 ketones, 3 acids, 2 furans, 1 pyrazine, and 1 sulfide. Meanwhile, principal component analysis demonstrates that GC‐IMS is an important tool for the rapid classification of hotpot seasonings. Finally, the sensory evaluation team concludes that the horse fat hotpot seasoning has promising development prospects. This study validates the applicability of GC‐IMS and GC‐MS for distinguishing VOCs in hotpots, and the findings provide a theoretical basis for flavor differentiation in HSOs.Practical Applications: Horse oil is significantly lower in cholesterol than butter, and thus possesses potential benefits for human health. This study provides a basis to produce horse oil hotpots and a reference for identifying the VOCs in HSOs.

Investigation of the Usability of Biodiesel from Horse Oil in Diesel Engines

Biodiesel is an alternative diesel fuel produced from animal and vegetable oils. As an alternative and ecologically acceptable substitute for conventional fuel, biodiesel is produced from a wide variety of edible vegetable oils that are usually used for human consumption and whose prices are expected to rise in the future. In this context, reliable and low-cost raw materials are gaining increasing interest for biodiesel production, such as by-products of meat processing industries or waste animal fats. Biodiesel production from waste animal fat, and raw food does not compete with the industry and has a great potential for waste caused by the global decline. In our study, a potential alternative fuel was produced for diesel engines by using the non-food-grade fat portion of horse meat consumed in Middle Asia countries. Solid crude horse oil was liquefied, and its fatty acid components were analyzed and transformed into horse oil biodiesel by the transesterification method. It was determined whether the fuel properties of crude horse oil, horse oil biodiesel, and euro diesel fuel comply with the standard values, and their usability in diesel engines was investigated. As a result of the tests, it has been concluded that horse oil biodiesel does not meet the standards in terms of cold flow properties and can only be used at a rate of low volumetric ratios in diesel engines. This article will contribute to the use of horse oil biodiesel production stages and fuel properties in diesel engines and future studies.

Extraction of Oleic Acid from Animals Oil and Its Anti-inflammatory Effect on Network Pharmacology

The purpose of this paper is to explore the possible mechanisms of anti-inflammatory and scar repair by Mongolian horse oil. We used TCM database and literature mining to collect active compounds of horse oil and used Swiss TargetPrediction and SuperPred server to find targets of compounds. Anti-inflammatory drug targets were collected through the CTD database. Go annotation of targets and KEGG pathway were enriched and analyzed through Metascape database platform. Molecular docking between active ingredients and targets was verified by AutoDock software. Metascape analysis revealed that the key candidate targets were significantly enriched in a number of pathways associated with inflammatory pathology. The results of molecular docking showed that oleic acid, a major component of animals oil, could influence the regulatory functions of TNF, NGF, IL6, IL1B, Jun, and CDK1. This suggests that animals oil can regulate the development of inflammation through its active ingredient, oleic acid, and can influence the expression of multiple signaling pathways, with theoretical endogenous interactions with TNF, NGF, IL6, IL1B, JUN, and CDK1 proteins.

Formulation and stability of horse oil-in-water emulsion by HLB system.

Optimal condition was determined to prepare horse oil-in-water (O/W) emulsion stabilized by different HLB system. Span 60 and Tween 60 were used to achieve the predetermined HLB values ranging from 10 to 14 and the surfactant concentrations were adjusted to 10-20%. Fifteen formulated O/W emulsions were characterized by mean particle diameter, zeta-potential (ZP), polydispersity index, and encapsulation efficiency (EE, %). Mean particle diameter decreased with increasing HLB value and surfactant concentration. Particles of the emulsion with HLB 12 and surfactant concentration at 15% were distributed in the size of below 500nm. The particle diameter and EE (%) of the emulsion with HLB 11 or 12 and surfactant concentration at 15 or 20% were not significantly changed during storage at 40°C for 15days. These results suggest the characteristics of horse oil O/W emulsion are dependent on HLB values and surfactant concentration so that affect to emulsion properties during storage.

A risk-based approach to produced water management in offshore oil and gas operations

Produced water is a waste of significant concern due to its high volume being produced every day and complex chemical composition. In order to meet environmental regulations and standards, different techniques can be used to treat produced water. This paper first summarizes produced water composition, its related environmental impact, regulations, and standards, as well as a possible combination of different treatment techniques. This paper aims to develop a generic framework for a risk-based approach to produced water management. The proposed methodology considers the integration of environmental, technical, and economic risks in the decision-making process for produced water management. Environmental risk assessment is conducted by DREAM, Failure Mode and Effects Analysis is used to estimate technical risk, and cost-benefit analysis is performed to calculate economic risk. To integrate all the risk values, acceptable risk levels are set and compared to the calculated risk values. Experts assign weighting factors by using pair-wise comparison. The sum of the multiplied weighting factors to the ratio of calculated-acceptable risk values gives the final integrated risk. This framework can help to examine and select the most suitable treatment or reuse technique or identify potential areas for improvement in a specific site. The estimated risk can be used to justify the selection process. A case study on the produced water treatment in Thunder Horse Oil Field is presented to demonstrate the application of the proposed framework.

Restoration of the inflammatory gene expression by horse oil in DNCB-treated mice skin.

The present study evaluated the anti-inflammatory effect of horse oil in 2, 4-dinitrochlorobenzene (DNCB)-treated BALB/c mice. After the application of DNCB, the mice showed atopic dermatitis symptoms, including severe erythema, hemorrhage, and erosion, whereas those symptoms were alleviated by treatment with horse oil. To explain the anti-dermatitis effect of horse oil, the gene expression levels in the healing process in dorsal skin were observed using a cDNA microarray. The cDNA microarray analysis revealed that the expression levels of 30 genes related to the inflammation, including Ccr1, Ccr2, Ccl20, Anxa1, and Hc genes, were up-regulated (higher than 2.0-fold) in the DNCB group compared to the levels in the control group, whereas the levels were restored to the control level in the DNCB + horse oil-treated group. In contrast, the gene expression levels of 28 genes related to inflammation, including chemokine genes Ccl5, Ccl7, Ccl8, Cxcl10, and Cxcl13 genes, were down-regulated (lower than 0.5-fold) in the DNCB group compared to the levels in the control group, whereas the levels were restored to the control level in the DNCB + horse oil-treated group. Overall, the results show that horse oil restores the expression levels of genes related to inflammation that were perturbed by DNCB treatment.

Physical Properties and Skin-Health Functions of Nonwoven Cosmetics Treated with Microcapsules Containing Citrus-Flavored Horse Oil

Physical Properties and Skin-Health Functions of Nonwoven Cosmetics Treated with Microcapsules Containing Citrus-Flavored Horse Oil

Anti-wrinkle and anti-inflammatory effects of a combination of topically applied horse oil and dietary enzyme hydrolysates from horse bone

This study investigated the anti-wrinkle and anti-inflammatory effects of horse bone hydrolysates of <3 kDa (HL) and topically applied horse oil (HO) on UVB-induced photoaging and inflammation in hairless mice. Forty-nine mice were divided into seven groups: −NC, chow without UVB irradiation; +UC, chow with UVB irradiation, +HLL, chow with low dose (500 mg/kg body weight (BW)) HL and UVB irradiation; +HLH, chow with high dose (1000 mg/kg BW) HL and UVB irradiation; +HO, chow and combined with topical application of HO and UVB irradiation; +HOHLL, treated with a combination of dietary HLL and topical application of HO with UVB irradiation; +HOHLH, treated with a combination of dietary HLH and topical application of HO with UVB irradiation. The transepidermal water loss in mice of all treatment groups was lower than that of mice in the +UC group. Furthermore, the wrinkle depth in the +HLH group was significantly lower than that in the +UC group. In addition, HO and low level of HL significantly decreased the level of tumor necrosis factor-α, interleukin-6, and matrix metalloproteinase-9, thereby down-regulating inflammation, including the cyclooxygenase-2 synthesis. In the future, human trials are needed to confirm the efficacy and synergistic effect of HL and HO.

Horse Oil Mitigates Oxidative Damage to Human HaCaT Keratinocytes Caused by Ultraviolet B Irradiation.

Horse oil products have been used in skin care for a long time in traditional medicine, but the biological effects of horse oil on the skin remain unclear. This study was conducted to evaluate the protective effect of horse oil on ultraviolet B (UVB)-induced oxidative stress in human HaCaT keratinocytes. Horse oil significantly reduced UVB-induced intracellular reactive oxygen species and intracellular oxidative damage to lipids, proteins, and DNA. Horse oil absorbed light in the UVB range of the electromagnetic spectrum and suppressed the generation of cyclobutane pyrimidine dimers, a photoproduct of UVB irradiation. Western blotting showed that horse oil increased the UVB-induced Bcl-2/Bax ratio, inhibited mitochondria-mediated apoptosis and matrix metalloproteinase expression, and altered mitogen-activated protein kinase signaling-related proteins. These effects were conferred by increased phosphorylation of extracellular signal-regulated kinase 1/2 and decreased phosphorylation of p38 and c-Jun N-terminal kinase 1/2. Additionally, horse oil reduced UVB-induced binding of activator protein 1 to the matrix metalloproteinase-1 promoter site. These results indicate that horse oil protects human HaCaT keratinocytes from UVB-induced oxidative stress by absorbing UVB radiation and removing reactive oxygen species, thereby protecting cells from structural damage and preventing cell death and aging. In conclusion, horse oil is a potential skin protectant against skin damage involving oxidative stress.

Quality Characteristics of Horse Oil Extracted by Hot Water with Adding Green Tea Leaves

Quality Characteristics of Horse Oil Extracted by Hot Water with Adding Green Tea Leaves

Two‐phase delivery using a horse oil and adenosine‐loaded dissolving microneedle patch for skin barrier restoration, moisturization, and wrinkle improvement

Dissolving microneedles (DMNs) have been used for skin restoration and wrinkle improvement. Although lipophilic compounds, for example, natural oils or ceramides, enrich the skin barrier, their delivery via DMNs is challenging because of DMN fabrication difficulties. In the present study, we combined a topical formulation and a DMN patch to perform two-phase delivery comprising a lipophilic formulation and hydrophilic compound-loaded DMNs to improve skin barrier status and the efficacy of drug delivery. Horse oil-spread and adenosine-loaded DMN arrays were developed in a single patch (HOS-Ad-DMN patch). In vitro analysis was conducted to confirm the successful delivery of the compositions. Clinical assessments were conducted on the lateral canthus of 20 women to compare the efficacy of HOS-Ad-DMN patches with that of adenosine-loaded DMN patches (Ad-DMN patches). Adenosine was delivered via the DMNs after skin penetration and horse oil was delivered successfully into the skin through the microchannels created by the Ad-DMNs. Compared with Ad-DMN patches, HOS-Ad-DMN patches significantly improved skin elasticity, hydration, dermal density, and wrinkles. No adverse events were observed. HOS-Ad-DMN patches are a safe and efficient system for skin restoration and wrinkle improvement.

A novel microbial technique for producing high-quality sophorolipids from horse oil suitable for cosmetic applications.

SummaryHorse oil contains linoleic, palmitoleic and unsaturated fatty acids that are similar to those in human skin, and may therefore be an ideal substance from which to isolate biosurfactants for cosmetic products to improve human skin quality. Herein, an innovative approach was developed to synthesise sophorolipids from horse oil by hydrolysis, followed by fermentation using the yeast Candida bombicola. The yield of sophorolipids from direct fermentation of horse oil and hydrolysed horse oil was 40.6 ± 1.3 g l−1 and 58.4 ± 1.8 g l−1 respectively. To further increase the yield, 30–40 g l−1 glucose was added in a fed‐batch fermentation process to maintain the pH between 4.0 and 4.5, resulting in a conversion yield of 71.7 ± 0.8 g l−1. The purity and structure of the synthesised sophorolipids were analysed by ultra‐performance liquid chromatography‐mass spectrometry and nuclear magnetic resonance. An in vitro human dermal fibroblast model was used as a surrogate for human skin to measure elastase inhibition activity. Antiwrinkle properties of isolated sophorolipids were better than those of horse oil or hydrolysed horse oil in several in vitro assays. Furthermore, no cytotoxicity was observed at a concentration of 50 μg ml−1, and wound‐healing capacity was evident in a cell culture model. Additionally, the synthesised sophorolipids attenuated lipopolysaccharide‐induced expression of inflammatory cytokines in macrophages, and efficiently inhibited several strains of bacteria and yeast. In conclusion, fed‐batch fermentation of hydrolysed horse oil is a novel and efficient approach for producing high‐quality and high‐yield sophorolipids that exhibit great potential as cosmetic ingredients.

Skin Barrier Restoration and Moisturization Using Horse Oil-Loaded Dissolving Microneedle Patches

Background: Horse oil (HO) has skin barrier restoration and skin-moisturizing effects. Although cream formulations have been used widely and safely, their limited penetration through the stratum corneum is a major obstacle to maximizing the cosmetic efficacy of HO. Therefore, we aimed to encapsulate HO in a cosmetic dissolving microneedle (DMN) for efficient transdermal delivery. Methods: To overcome these limitations of skin permeation, HO-loaded DMN (HO-DMN) patches were developed and evaluated for their efficacy and safety using in vitro and clinical studies. Results: Despite the lipophilic nature of HO, the HO-DMN patches had a sharp shape and uniform array, with an average length and tip diameter of 388.36 ± 16.73 and 38.54 ± 5.29 µm, respectively. The mechanical strength of the HO-DMN patches was sufficient (fracture force of 0.29 ± 0.01 N), and they could successfully penetrate pig skin. During the 4-week clinical evaluation, HO-DMN patches caused significant improvements in skin and dermal density, skin elasticity, and moisturization. Additionally, a brief safety assessment showed that the HO-DMN patches induced negligible adverse events. Conclusion: The HO-DMNs are efficient, safe, and convenient for wide use in cosmetic applications for skin barrier restoration and moisturization.

External Application of Horse Oil Alleviates Atopic Dermatitis Symptoms in Mice

Atopic dermatitis is characterized by skin barrier dysfunction, edema, and infiltration with various inflammatory cells. Horse oil,the purified form from horse fat, has been used for a long time by fork remedies on burn, wound, and various skin diseases. Inthis study, we investigated the improving effects of horse oil on atopic dermatitis induced by 0.3% of DNCB in mice. Horse oil B(oil phase, 100 mg/kg) significantly decreased serum IgE levels in experimental atopic dermatitis mice induced by DNCB. Also,horse oil A (cream phase, 100 mg/kg) and B alleviates atopic dermatitis symptoms (ear edema, inflammatory cell infiltration, andlymph node size). Overall, the effect of horse oil B was slightly better. These results suggest that horse oil can be a usefulremedy to improve atopic dermatitis.

Antioxidant and Antiproliferative Activities of Purslane Seed Oil.

The aim of this study was to evaluate the antioxidant and antiproliferative activities of PSO in vitro and its application in horse oil storage. We determined the reducing power of PSO and its scavenging effects on hydroxyl (•OH) and 1,1-diphenyl-2-picrylhydrazyl radicals (DPPH•) and tested its stabilizing effects on horse oil storage. The results showed that PSO had remarkable, dose-dependent antioxidant activities, and it effectively prevented horse oil lipid oxidation. We treated cervical cancer HeLa cells, esophageal cancer Eca-109 cells and breast cancer MCF-7 cells with PSO using non-neoplastic monkey kidney Vero cells as controls. The results indicate that PSO significantly inhibited tumor cell growth in a time- and dose-dependent fashion. Our studies suggest that PSO may be used as a substitute for synthetic antioxidants in food preservation and may be potentially useful as a food and cosmetic ingredient. Meanwhile, the oxidative stress can cause hypertension, so PSO is expected to develop a health care products for the prevention and mitigation hypertensive symptoms.

How the choice of biofuel system in a small-scale Swedish organic farm affects food production and NPK balance

Modern agriculture is causing extensive environmental damage. Arguments have been put forward that a systemic change is needed in agriculture to address the major environmental challenges faced by mankind. This is a case study of a research farm whose aim is to form a farming system that has a minimal impact on carbon and nitrogen cycles while maintaining biodiversity. For the farm to minimise its impact on the global carbon cycle and associated climate change, it is important to reduce fossil energy use. The research farm has minimised all its inputs, but uses a diesel-run tractor. Agriculture's primary function is to provide food of adequate quality. The hypothesis of this study is that using biomass from arable land for tractor work on the farm would reduce the amount of food produced relative to using fossil fuel for tractor work. The objective of this paper was to develop scenarios for biomass-derived tractor work for the study farm chosen and to investigate their impact on food production. Food availability was measured by the number of people the farm could supply, denoted as Np. A farm nitrogen, phosphorus and potassium (NPK) balance was also applied to the different scenarios. The investigated scenarios for replacing fossil fuel tractor work were draught horsepower combined with cold-pressed rape-seed oil in a tractor, ethanol from wheat produced in a conventional ethanol plant, and farm-made ethanol from potatoes. Using wheat and potato to produce ethanol reduced food production by 23% and 18% respectively, compared to using conventional diesel. Combining a draught horse and on-farm cold-pressed rapeseed oil only reduced the food production by 6%. N is maintained in all scenarios due to the nitrogen-fixing leys. P can be maintained in the fields if bones are recycled, but nutrients, especially K, are moved from meadow to field.

마유(馬油)가 DNCB로 유발된 알레르기성 접촉성피부염에 미치는 영향

Objectives : Horse oil (HO) has been used long time as the folk medicine of many Asian countries such as Korea, Mongol, China, India and Japan. HO has been used for anti-bacterial, anti-inflammatory, and anti-pruritic purposes in skin. However, it is still largely unknown whether HO modulates the skin condition. In this study, we attempted to evaluate the anti-inflammatory effect of HO on the 1 % of 2, 4-dinitro-1-chlorobenzene (DNCB)-induced contact hypersensitivity in Balb/c mice. Methods : To find the anti-inflammatory effect of HO, contact hypersensitivity, a local inflammatory response of skin, was induced on the back of Balb/c mice by sensitization and repeated application by 1% DNCB and HO treated 2 weeks on the 1% of DNCB-treated Balb/c mice. Excised mice skins were stained with hematoxylin and eosin and serum IgE level was measured by mouse IgE ELISA kit. Results : In this study, we found that HO reduced erythema by 1% of DNCB treated Balb/c mice. Also, HO recovered histopathological features such as the thickening of epidermis, hyperkeratosis and the infiltration of inflammatory cells in 1% of DNCB treated Balb/c mice. In addition, HO reduced IgE level on the serum obtained from blood of 1% of DNCB-treated Balb/c mice. Conclusion : Taken together, these results showed that HO could be used as a pharmaceutical material with anti-inflammatory effects by reducing of erythema, IgE level and recovering of histopathological features skin on DNCB-induced contact hypersensitivity in Balb/c mice model.

Horse Oil Effect on the Skin by Topical Application

Horse Oil Effect on the Skin by Topical Application

Effects of ovarian steroids on luteal function: prevention of luteolysis following LH-neutralization in the pseudopregnant rat.

AbstractThe ability of estradiol (E) and progesterone (P) to act as luteotrophins in place of LH was investigated in pseudopregnant (PSP) rats. The length of PSP was extended by either hysterectomy (hyst.) or by the experimental induction of decidual tissue (DT) on Day 5 (Day 1 = ovulation). On Day 9, LH was removed from circulation by a sc injection of a specific LH antiserum (LHAS) and each rat was simultaneously treated with various dosages of E and/or P. Control rats were treated with normal horse serum (NHS) and/or oil vehicle. Blood samples were collected at 0, 24, and 72 hr post-treatment for the estimation of serum P levels. Day 5 hyst. rats treated with any dose (0.1 − 10.0 μg) of E or P (2 mg), alone or in combination, and simultaneously injected with NHS or LHAS exhibited elevated P levels through Day 12 and an extended diestrous cycle. In contrast, oil-treated, LHAS-injected rats underwent immediate luteolysis. DT-bearing PSP rats treated with 10 μg of E failed to undergo luteolysis following ...