High Level Of Antibacterial Activity Research Articles

Phytochemical and Antibacterial Study of Ethanol and Ethyl acetate Extracts from Leaves of Alchornea Cordifolia Against Isolates from Infected Wounds

Alchornea cordifolia leaves have been reported to be used traditionally to treat wound infections. The leaves ofAlchornea cordifolia were extracted with ethanol which was partitioned with ethyl acetate to obtain the ethanolextract and ethyl acetate fraction respectively. The extracts were screened for basic phytocompounds and subjectedto antibacterial assay against bacterial isolates from infected wounds which include Pseudomonas aeruginosa,Staphylococcus aureus, Klebsiella spp, Enterobacter spp, Citrobacter spp, Escherichia coli, Proteus mirabilis andProteus vulgaris. Agar well diffusion method was used to determine the antibacterial activity of the extract andfraction. Phytochemical investigation revealed the presence of carbohydrates, cardiac glycosides, saponins,flavonoids, tannins and alkaloids. The zone of inhibition ranged between 1.0 - 32.2mm for the entiremicroorganisms. This variation in level of the activity among the extracts could be due to the difference in solubilityof the active ingredient in each solvent on one hand and to the constitutional or structural variability of the testedorganisms on the other hand. The results of the study showed that the ethyl acetate fraction of Alchornea cordifolialeaves possess high level of antibacterial activity against bacteria associated with wound infection. This givescredence to traditional medicine application of the plant for treating wound infections.

Anti-Oxidant, Anti-Bacterial and Anti-Cancer Activity of Mentha Piperita Against Mcf-7 Cells

The study evaluates the antioxidant, antibacterial and anticancer activities of different extracts of Menthapiperitaleaves against the MCF-7 cell line. The leaves of Menthapiperitawere extracted with aqueous, ethyl acetate, and chloroform. These extracts were subjected to qualitative phytochemical screening, antibacterial activity, cytotoxic activity, and AO/ErBr assay for cells' apoptotic effect against the MCF-7 cell line. Qualitative analysis of the leaves' different extracts revealed glycosides, alkaloids, flavonoids, terpenoids, tannin, and saponin. The antibacterial activity of the leaf extracts was examined against four different bacterial species (Bacillus cereus, Pseudomonasfluorescens, Aeromonas hydrophila, and Klebsiella pneumoniae). The aqueous extract exhibited a high level of antibacterial activity (18.66mm ±1.1 in Bacillus cereus).Free‑radicalscavenging activity of chloroform extractof M. piperita leaves was found to be more than aqueous and ethyl acetate extracts. Further, aqueous, ethyl acetate,and chloroform extracts exerted a cytotoxic effect with the IC50value of 45±1.5μg/ml, 29±1.2μg/ml, and 24±1.0μg/ml, respectively.From this study, we have observed that chloroform extract showed a concentration-dependent apoptotic effect against MCF-7 cellsdetermined by AO/EtBr assay.The resultsfurther depicted that the selected traditionalMentha piperita could be used as a potential anticancer, antibacterial, and good antioxidant agent against the MCF-7 cell line.

X- ray diffraction Characterization of crystalline structure regent4(4-Sulphophenyl Azo) Pyrogallol

New azo reagent was synthesized by reaction dizonium salt Sulfadiazine with Pyrogallol. This product was characterized by FTIR, UV. Visible spectrophotometer and (XRD). Purity of the dye was checked by thin layer chromatography (TLC) using solvent system (Toluene-Methanol) (2:3). The melting point of the purified dye was measured in an open capillary tube. The results showed this compound have high levels of antibacterial activity.

Preparation, Characterization and Prevention Biological pollution of 4 (4-Benzophenylazo) Pyrogallol and their Metal Complexes

New azo reagent was synthesized by reaction dizonium salt of Para aminobenzoic acid with Pyrogallol, also prepared three Chelate complexes of this reagent with the metal ions of Co(II), Ni(II), and Ag(I). This reagent was characterized by FTIR(Fourier Transform Infrared), UV. Visible spectrophotometer, FESEM and HNMR proton. The antibacterial activities of the compounds were studied and evaluated using gram positive and negative gram bacteria. The purity of the dye was checked by thin layer chromatography (TLC) using solvent system (Toluene-Methanol) (2:3).The melting point of the purified dye was measured in an open capillary tube. The results showed that some of these compounds have high levels of antibacterial activity.

Антибактериальная активность хлоргексидина биглюконата по отношению к биопленочным монокультурам

On the background of a high percentage of caries incidence, the prevalence of its complications is increasing – pulpitis, apical periodontitis. Teeth with complicated caries can cause odontogenic inflammatory processes in the maxillofacial region. Being foci of chronic infection and intoxication, they have an adverse effect on the body as a whole. In connection with the above, it is obvious that determining the optimal concentration of chlorhexidine bigluconate and exposure to the dentin of the crown part of the tooth in case of carious lesion of the latter is currently very important. The purpose of the study was to evaluate the antimicrobial activity of chlorhexidine (by suspension and contact method) in various concentrations in relation to monocultures of biofilm formed on tooth sections and in U-shaped 96-well plastic plates. Material and methods. In this work, one of the most relevant pathogens for modern medicine was Staphylococcus aureus, which was one of the most powerful pathogens that form biofilms, was used as a model. The antimicrobial activity of chlorhexidine bigluconate was evaluated on S. aureus and E. coli biofilm cultures formed on dental samples and in polystyrene plastic tablets. Antimicrobial activity was evaluated by the reduction factor was determined by the difference in the number of decimal logarithms of CFU/ml in the experiment compared to the control. The obtained data was subjected to statistical processing. Results and discussion. The study revealed that 2.0 % solution of chlorhexidine bigluconate had a high level of antibacterial activity against tablet biofilm cultures of S. aureus and E. coli. When it was exposed to S. aureus and E. coli tablet biofilm cultures with exposures of 30 and 60 seconds, statistically significant differences were found (p=0.02). Secondly, statistically significant differences in the effect of sensitivity of single-species biofilm cultures of dental samples to the effect of antiseptic at the specified concentration were determined (p=0.007). At the same time, the level of antibacterial activity of 2.0 % chlorhexidine bigluconate solution in respect of tablet biofilm cultures of S. aureus and E. coli was significantly higher than in biofilm cultures of S. aureus and E. coli of dental samples (p<0.05). Conclusion. The obtained results give grounds for using them in clinical practice in order to improve the effectiveness of caries treatment which will help reduce the number of complications and on the one hand, corresponds to the main direction of medicine ‒ prevention and on the other hand, increases the level of specialized medical care provided to the population as a whole

1,2,3-Triazole-gold(I)-triethylposphine derivatives active against resistant Gram-positive pathogens

Innovative organogold(I) antibacterial compounds were synthesized by click chemistry with triethylphosphine-gold(I) azides and an alkyne derivative. The resulting organo-gold(I) compounds exhibit high levels of antibacterial activity against Gram-positive pathogens, with particularly low MICs against Clostridium difficile.

Bioinspired and eco-friendly high efficacy cinnamaldehyde antibacterial surfaces.

Antimicrobial essential oils are incorporated into mussel-inspired and natural plant polyphenol coatings as part of a single-step fabrication process. Polydopamine-cinnamaldehyde, polyethyleneimine-cinnamaldehyde, and tannic acid-cinnamaldehyde coatings exhibit strong antibacterial activities against both Gram-negative Escherichia coli and Gram-positive Staphylococcus aureus (with the polydopamine- and tannic acid-based systems displaying log10 Reduction = 8). Cinnamaldehyde impregnation into porous non-woven polypropylene cloth, polytetrafluoroethylene membrane, and knitted cotton cloth also gives rise to high levels of antibacterial activity (log10 Reduction = 8). No loss in antibacterial efficacy is observed for non-woven polypropylene cloth impregnated with cinnamaldehyde over 17 recycle tests.

Bacterial Profile and Antimicrobial Susceptibility Pattern of Chronic Suppurative Otitis Media from a Tertiary Care Hospital in Kannauj, Uttar Pradesh, India

Introduction: Chronic Suppurative Otitis Media (CSOM) is a chronic and persistent inflammation of the mucosal lining of middle ear cleft which presents with recurrent ear discharge through a tympanic membrane perforation. CSOM is a worldwide disease and has higher prevalence in developing countries causing serious local damage and threatening complications. Aim: To determine the profile of CSOM, its causative agents and their antibiotic susceptibility pattern. Materials and Methods: The present study was a retrospective study in which data for culture and antimicrobial susceptibility profile of CSOM cases was retrieved from Microbiology department of Government Medical College, Kannauj, Uttar Pradesh, India, from January 2019 to October 2019 the analysis of data was done in February 2020. Two hundred pus swabs from discharging ears were processed during the study period for aerobic bacterial culture and antimicrobial susceptibility pattern in Microbiology department using standard microbiological techniques. The result analysis of the study was expressed as percentage and proportion for distribution of CSOM cases. Results: Out of 200 patients, 114 (57%) were males and 86 (43%) females. Among the 200 cases, 147 (73.5%) cases showed growth and 53 (26.5%) showed no growth. The most common organism isolated in this study was Pseudomonas aeruginosa, 67 (42.40%) followed by Staphylococcus aureus.48 (30.37%) and Klebsiella spp. 23 (14.55%). Gentamicin (85%) and amikacin (83%) had high level of antibacterial activity on all the isolates. Meropenem was found to be most effective drug for gram negative isolates. All Staphylococcus spp isolates were sensitive to vancomycin (100%). Conclusion:Pseudomonas aeruginosa was the most predominant organism isolated in otitis media patients. Most of the isolates were sensitive to meropenem.

Antimicrobial Efficacy of a Novel Antibiotic-Eluting Injectable Platelet-Rich Fibrin Scaffold against a Dual-Species Biofilm in an Infected Immature Root Canal Model.

Background and Aims This study was aimed at evaluating the antibacterial property of an injectable platelet-rich fibrin (I-PRF) scaffold containing triple antibiotic mixture against an Actinomyces naeslundii (A. naeslundii) and Enterococcus faecalis (E. faecalis) biofilm in an infected immature root canal model. Methods A dual-species biofilm was inoculated inside the root canals via a series of centrifugal cycles. The samples were allocated to three experimental groups (i.e., G1: triple antibiotic mixture, G2: I-PRF containing triple antibiotic mixture, and G3: antibiotic-free I-PRF scaffold) and two control groups (G4: seven-day biofilm untreated and G5: bacteria-free untreated). Results Bacterial gene quantification change and the overall reduction of live bacteria were evaluated. The highest antibacterial activity against A. naeslundii belonged to G2. However, G1 and G2 had similar antibacterial property against E. faecalis (p value = 0.814). In general, experimental groups revealed higher levels of antibacterial activity against E. faecalis than against A. naeslundii (p value < 0.001). Notably, G2 could dramatically decrease the number of live bacteria up to near 92%. Conclusions The current study provides insight into the antibacterial property of an antibiotic-eluting I-PRF scaffold against a dual-species biofilm colonized inside the root canal. The fabricated scaffold contains not only the antibiotics but also the growth factors, which favor the regeneration.

Effect of extraction temperature and solvent type on the bioactive potential of Ocimum gratissimum L. extracts

Ocimum gratissimum is a shrub that belongs to the Lamiaceae family of plants. Despite the known biological activities and ethnomedicinal applications, comparative evaluation of the effects of different extraction techniques on the chemical and bioactive properties of O. gratissimum extracts has not yet been performed. This study adopted different analytical techniques to determine the effect of extraction temperature and solvent type on the phytochemical and bioactive properties of O. gratissimum extracts. Chemical profiling showed increased concentrations of compounds for both the ethanolic and methanolic extracts compared to the water extracts. The results also revealed that the extraction temperature had an effect on the total phenolic content and radical-scavenging properties of the different extracts. The antioxidant kinetic modeling achieved the best fit when using the second-order kinetic model. Methanolic extracts had the highest levels of antibacterial activity against Escherichia coli, Bacillus cereus, Staphylococcus aureus, and Salmonella typhimurium. At high concentrations, all extracts lowered the viability of the breast cancer cell line MDA-MB-231. In conclusion, the chemical and bioactive properties of all extracts showed significant dependence on the extraction temperature and solvent type. With proper extraction methods, they boast a wide range of promising applications in the medical, pharmaceutical, and food industries.

In vitro and in vivo evaluation of structurally-controlled silk fibroin coatings for orthopedic infection and in-situ osteogenesis

Biomedical device-associated infections (BAI) and osteosynthesis are two main complications following the orthopedic implant surgery, especially while infecting bacteria form a mature biofilm, which can protect the organisms from the host immune system and antibiotic therapy. Comparing with the single antibiotics therapeutic method, the combination of silver nanoparticles (AgNPs) and conventional antibiotics exert a high level of antibacterial activity. Nevertheless, one major issue that extremely restricts the potential application of AgNP/antiviotics is the uncontrolled release. Moreover, the lack of osteogenic ability may cause the osteosynthesis. Thus, herein we fabricated a structure-controlled drug-loaded silk fibroin (SF) coating that can achieve the size and release control of AgNPs and high efficient osteogenesis. Three comparative SF-based coatings were fabricated: α-structured coating (α-helices 32.7%,), m-structured coating (β-sheets 28.3%) and β-structured coating (β-sheets 41%). Owning to the high content of α-helices structure and small AgNPs (20 nm), α-structured coating displayed better protein adsorption and hydrophilicity, as well as pH-dependent and long-lasting antibacterial performance. In vitro studies demonstrated that α coating showed biocompatibility (cellular attachment, spreading and proliferation), high ALP expression, collagen secretion and calcium mineralization. Moreover, after one month subcutaneous implantation in vivo, α-structured coating elicited minimal, comparable inflammatory response. Additionally, in a rabbit femoral defect model, α-structured coating displayed a significant improvement on the generation of new-born bone and bonding between the new bone and the tissue, implying a rapid and durable osteointegration. Expectedly, this optimized structure-controlled SF-based coating can be an alternative and prospective solution for the current challenges in orthopedics. Statement of SignificanceIn this study, an AgNPs/Gentamycin-loaded structured-controlled silk fibroin coatings were constructed on Ti implant's surface to guarantee the success of implantation even in the face of bacterial infection. In comparison, the α-structured coating had the lowest content of β-sheets structure (19.0%) and the smallest particle size of AgNPs (~ 20 nm), and owned pH-responsive characteristic due to reversible α-helices structural. Thanks to pH-responsive release of Ag+, the α-structure coating could effectively inhibit adhesive bacteria and kill planktonic bacteria by releasing a large amount of reactive oxygen radicals. Through in vitro biological results (cell proliferation, differentiation and osteogenic gene expression) and in vivo rabbit femur implantation results, the α-structure coating had good biocompatible and osteogenic properties.

Studies of new biocidal polyguanidines: antibacterial action and toxicity

The aim of this study was to obtain new water-soluble polyguanidines, assess their antimicrobial properties with respect to the test objects of Escherichia coli and Bacillus cereus and establish the hazard class regulated in the territory of the Russian Federation. Polymers obtained in high yields by the method of polycondensation in the melt of the corresponding monomers and characterized by the methods of IR spectroscopy and elemental analysis. Serial dilution–agar plate procedure was performed in order to estimate antibacterial effects. The biocidal properties of the samples were estimated by the percentage reduction of microorganisms (% reduction). To determine the acute toxicity, a toxicometric assessment and symptoms of intoxication upon oral administration of a single dose were carried out in laboratory animals. New water-soluble guanidine-containing polymers with a number average molecular weight of 660–1040 Da were obtained. Copolymers with a low degree of substitution (10–20%) exhibit high antimicrobial activity among N-phenyl-substituted samples. As a result of the substituent increase in the polymer chain, their activity in relation to the studied bacteria is linearly decreased to a level comparable to the test sample. In contrast to the N-octyl-substituted copolymers, gram-positive bacterium B. cereus is more susceptible to N-phenyl-substituted samples. Substances after intragastric injection belong to the fourth class of toxicity (LD50 > 300 ≤ 2000 mg/kg) according to the requirements of tabulation of toxicity classes regulated in the territory of the Russian Federation. The combination of low toxicity with a high level of antibacterial activity of new guanidine-containing polymers suggests the possibility of obtaining new effective disinfectants.

Evaluation of Structure-Controlled Silk Fibroin Coatings for Orthopedic Infection and In-Situ Osteogenesis: &lt;i&gt;In Vitro&lt;/i&gt; and &lt;i&gt;In Vivo&lt;/i&gt;

Biomedical device-associated infections (BAI) and osteosynthesis are two main complications following the orthopedic implant surgery, especially while infecting bacteria form a mature biofilm, which can protect the organisms from the host immune system and antibiotic therapy. Comparing with the single antibiotics therapeutic method, the combination of silver nanoparticles (AgNPs) and conventional antibiotics exert a high level of antibacterial activity. Nevertheless, one major issue that extremely restricts the potential application of AgNP/antiviotics is the uncontrolled release. Moreover, the lack of osteogenic ability may cause the osteosynthesis. Thus, herein we fabricated a structure-controlled drug-loaded silk fibroin (SF) coating that can achieve the size and release control of AgNPs and high efficient osteogenesis. Three comparative SF-based coatings were fabricated: α-structured coating (α-helices 32.7%,), m-structured coating (β-sheets 28.3%) and β-structured coating (β-sheets 41%). Owning to the high content of α-helices structure and small AgNPs (20 nm), α-structured coating displayed better protein adsorption and hydrophilicity, as well as pH-dependent and long-lasting antibacterial performance. In vitro studies demonstrated that α coating showed good biocompatibility (cellular attachment, spreading and proliferation), high ALP expression, collagen secretion and calcium mineralization. Moreover, after one month subcutaneous implantation in vivo, α-structured coating elicited minimal, comparable inflammatory response. Additionally, in a rabbit femoral defect model, α-structured coating displayed a significant improvement on the generation of new-born bone and bonding between the new bone and the tissue, implying a rapid and durable osteointegration. Expectedly, this optimized structure-controlled SF-based coating can be an alternative and prospective solution for the current challenges in orthopedics.

Recombinant expression and functional analysis of antimicrobial Siganus oraminl-amino acid oxidase using the Bac-to-Bac baculovirus expression system

Siganus oraminl-amino acid oxidase (SR-LAAO), isolated from the serum of Siganus oramin, is an innate immune protein with significant antibacterial activity. The aim of this study was to express SR-LAAO in insect cells using a baculovirus expression system and evaluate the function of the recombinant SR-LAAO. To this end, an optimized sequence of the SR-LAAO gene was designed and synthesized, based on the codon bias of insect cells. Bacmid shuttle vectors and recombinant baculovirus were successfully constructed, and the recombinant baculovirus was transfected into Sf9 insect cells. The antibacterial activity and enzymatic characteristics of the recombinant SR-LAAO were investigated. The results showed that the pFastBac-optiSR-LAAO shuttle vectors and Bacmid-optiSR-LAAO were correctly constructed. The Sf9 insect cells exhibited significant cytopathic effects following infection with Bacmid-optiSR-LAAO and Bacmid; the specific PCR analysis proved that the recombinant baculovirus was successfully constructed. The immunofluorescence assay revealed that the recombinant baculovirus rSR-LAAO was abundantly expressed in infected Sf9 insect cells; the results of SDS/PAGE and Western blot analyses showed that a specific band appeared at about 60 kDa. Moreover, the crude rSR-LAAO enzyme displayed strong antibacterial activity against aquatic pathogens, particularly Streptococcus agalactiae and Streptococcus iniae. In addition, the results of catalase interference test implied that the antibacterial activity of rSR-LAAO was directly associated with (H2O2 production). The results of the rSR-LAAO enzymatic characteristics test indicated that the Km value with l-Lysine as a substrate was 16.61 mM when the temperature was under 37 °C, and the optimum pH was 7. The antibacterial activity of rSR-LAAO could be completely inhibited by 10 mg/mL of pepsin, trypsin, and proteinase K compared with both methanol and acetone. Adding an equal volume of ethanol had a minimal impact on the antibacterial activity of rSR-LAAO. The crude enzyme could maintain a high level of antibacterial activity against both Gram-positive and -negative bacteria from 4 °C to 30 °C. In the present study, SR-LAAO was successfully expressed in Sf9 cells using the baculovirus expression system, and provides basic references for further research into the role of LAAO in marine animals and the development of new antimicrobial drugs.

Antibacterial and Antioxidant Characteristics of Zataria multiflora Boiss Essential Oil and Hydroalcoholic Extract of Rhus coriaria L.

Background: The increasing demand for natural preservatives results in their extended usefulness. The objective of the present study was to investigate the physicochemical and antioxidative characteristics of Rhus coriaria L. (sumac) fruit and comparison of its antioxidative and antibacterial activity with Zataria multiflora Essential Oil (ZEO) as native Iranian natural additives. Methods: Antioxidant activities of Z. multiflora Boiss and sumac were analyzed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging, 2, 2'-azino-bis 3-ethylbenzothiazoline-6-sulphonic acid (ABTS). Reducing power tests were used for measuring antioxidant activity. Total phenolic content of extract and essential oil were studied as well. The Minimal Inhibitory Concentration (MIC), Minimal Bactericidal Concentration (MBC), and Fractional Inhibitory Concentration (FIC) of a hydroalcoholic extract of sumac and ZEO against of Salmonella Typhimurium and Listeria monocytogenes were studied. Statistical analysis of data was performed using the SPSS software. Results: The phenolic content in sumac extract (305.65 mg/g) was significantly (p˂0.05) higher than ZEO (179.42 mg/100 g). The highest level of antibacterial activity was demonstrated by ZEO with the MICs of 0.625 for S. Typhimurium and 1.25 mg/ml for L. monocytogenes. Conclusion: Sumac extract showed more potent antioxidative activity than ZEO. However, based on the results of antibacterial activity, ZEO had more potent than sumac extract, significantly.

Antibacterial Effects and Synthesized New Derivatives of 4-Hydroxy-Chromen-2-One

In present paper, we report the organic syntheses of three compounds exactly 4-hydroxy-chromen-2-one and describe the results of antibacterial activity of purified compounds. Compounds 4-(4-Methoxy-phenylamino)-2-oxo-2H-chromene-3-sulfonyl chloride (a). N-{3-[Diethoxy-(2-hydroxy-phenyl)-methyl]-2-oxo-2H-chromen-4-yl}-acetamide (b), 4-(6-Nitro-benzothiazol-2-ylamino)-2-oxo-2H-chromene-3-carbaldehyde (c), have been synthesized and characterized using melting points, IR spectra, 1H-NMR and 13C-NMR spectra. Our research goal is synthesis novel organic compounds which prove high level of antibacterial activity. The antibacterial activity of synthesized compounds and streptomycin and cefalexine at concentrations of 2mg/ml, 3mg/ml and 5mg/ml, have been evaluated against three strains of bacterial culture; Staphylococcus aureus, E. coli and Bacillus cereus. The compounds show bacteriostatic and bactericidal activity in high level and also synthetized compounds has been characterized using advanced instrumental methods of analysis.

Compositions of essential oils with antimicrobial properties against isolates from oral cavities of patients with inflammatory diseases of the periodontium

The significant spread of antibiotic-resistant microorganisms which are part of microbial associations of the oral cavity is considered one of the main causes for the complications and relapses of inflammatory diseases of the periodontium. This problem underlines the importance of constant monitoring of the circulation of polyantibiotic-resistant isolates, and development of new approaches and means of correction for the microbiocoenosis of the oral cavity affected by inflammatory processes. The paper is dedicated to research of antimicrobial properties of essential oil compositions against opportunistic pathogenic microorganisms, agents of inflammatory diseases of the periodontium. Out of the microorganisms isolated from the nidus of inflammation, the extra antibiotic-resistant ones were chosen. For the purpose of antibiotic susceptibility and antimicrobial activity testing, the disc diffusion method was used. The following compositions were manufactured from essential oils: Hyssopus officinalis L. + Rossmarinus officinalis L.; Menta piperita L. + H. officinalis; R. officinalis + Coriandrum sativum L.; R. officinalis + M. piperita + H. officinalis. The compositions H. officinalis + R. officinalis; M. piperita + H. officinalis were shown to have the highest level of antibacterial activity against clinical and typical isolates. Combinations of essential oils demonstrated a broader spectrum of activity against microorganisms than their components taken separately. Certain essential oils and the compositions H. officinalis + R. officinalis; M. piperita + H. officinalis were ascertained to have a high anti-mycotic activity against Candida genus microscopic fungi. This fact proves the advantage of application of combinations of essential oils compared to the use of their separate components. However, not all variants reviewed showed additive antimicrobial effect of the use of combinations of essential oils. The compositions that showed high antibacterial properties against the reviewed isolates may be used for oral hygiene products.

Antibacterial effects of Schisandra chinensis extract on Staphylococcus aureus and its application in food

Abstract Schisandra chinensis (Turcz.) Baill is a member of the Magnoliaceae and is used in traditional medicine. Its extracts have antibacterial activity against foodborne pathogens. In present study, the antibacterial properties of Sc. chinensis were tested on S.aureus. An in vitro antibacterial assay showed that Sc. chinensis extracts (SCE) inhibited the growth of test bacteria at a MBC of 18mg mL−1. SCE demonstrated strong antibacterial activity in barley soup system. Treated by SCE, the growth curves of S.aureus exhibited extended lag phases and abbreviated log phases, S. aureus presented with wrinkled and withered surfaces and surrounded by soluble substances probably consisting of leaked intracellular materials. Further, S. aureus released the periplasmic enzyme (AKPase) and increased activities of membrane‐bound Na+/K+/ Ca++‐ATPase. Therefore, SCE can damage S. aureus cell membranes and walls. Moreover, SCE decreased dehydrogenase and total ATPase activities, and intracellular protein contents, even interacted with DNA by groove binding.Practical applicationsIn a barley soup model food system, SCE possessed a high level of antibacterial activity and significantly reduced total viable bacterial counts. This study laid the theoretical foundation for the application of Schisandra chinensis extract in preventing the growth of foodborne pathogens.

An Economical Dyeing Process for Cotton and Wool Fabrics and Improvement their Antibacterial Properties and UV protection

ABSTRACTEnvironmental, economic concerns, and prolonged human exposure to solar ultraviolet radiation have created an increasing demand for natural dyestuff. A study on the cotton and wool fabrics functionality to achieve UV protection and antibacterial properties was carried out by aqueous extract of barberry roots and red onion skin as the natural dyes. The alkaloid berberine extracted from the fruits of Berberis vulgaris and flavonoids and anthocyanin from red onion skin (Alliumcepa L) are natural dyes with antibacterial activity, UV protection and available as waste materials. The properties of dyed wool and cotton fabrics with these two natural dyes and two dyeing methods (exhaustion and ultrasonic) such as their color fastness, color intensity, and antibacterial efficacy ultraviolet protection factor (UPF) were investigated in this research. The results confirmed that the high level of antibacterial activity and (UPF) values of dyed wool fabrics with barberry roots and red onion skin and they are more than the dyed cotton fabrics in the same condition and has better result with the ultrasonic method. So by combining two dyestuff and ultrasonic method, on cotton dyeing, the level of antibacterial activity and UPF value increased.

Study of antibacterial properties of honey to methycilin resistant Staphylococcus

For publishing this article, two important ideas were indicated: the first one is the necessity of scientific research to establish antibacterial properties in those substances that can replace antibiotics in the fight against antibiotic resistant microorganisms; the second is to expand the possibilities for using a national Ukrainian product, such as honey. Ukraine is one of the important exporters of honey in the EU. This honey may have unique physical and chemical properties that will characterize its therapeutic effect. In other countries, these issues are actively studied by scientists, which provide special healing brands to certain its species. These issues are not well study in Ukraine. The aim of present study was to an experimental study of the use of antibacterial properties of honey in relation to methicillin-resistant Staphylococcus spp. Materials for research were isolates of methicillin-resistant Staphylococcus spp. (MRSS) and samples of natural honey that were obtained from apiaries of the Odessa region. The standard methods of isolation Staphylococcus spp and methods determining the quality of honey were used. Qualitative indicators of honey included organoleptic studies, moisture and acidity, proline content. Determination of antibacterial properties of honey was studied in dilution it in distilled water in proportions 1:1, 1:2, 2:1 and 3:1 with following plating in nutrient agar wells on Petri dishes. It has been experimentally established that honey may be an excellent remedy against MRSS, but not all honey has the same antimicrobial activity. It has been established that the antibacterial properties of honey in relation to MRSS are influenced by the physical and chemical composition, namely the content of proline. It has been experimentally established that most types of investigated honey samples with high qualitative indices exhibited antibacterial action against antibiotic-resistant isolates of Staphylococcus spp., With higher levels of antibacterial activity showing those honey samples that had a higher proline content of more than 350 mg/kg. It was established that the antibacterial action of honey against antibiotic resistant isolates of Staphylococcus spp. It is better manifested in more concentrated solutions – when diluted in the ratio of honey / solvent as 2:1 and 3:1 (growth retardation zones from 34 mm to 58 mm) compared to solutions of honey diluted in proportions 1:1 and 1:2 (growth retardation zones from 10 mm to 15 mm).