ERG impulse response, amplitude and phase temporal spectral transfer functions, and nonlinearities were measured in turtle retina under different retinal temperatures, pharmacological treatments, and light intensities.b-Wave amplitude is strongly temperature dependent; amplitudes of thea-wave and slow P-III are less sensitive. Their time courses all slow markedly as temperature decreases. ERG amplitude transfer function is bimodal bandpass with narrow low-frequency peak (below 1 Hz) and broader mid-frequency peak (5 Hz at 8°C). Both peaks broaden and their frequency increases (low, 1 Hz; mid, 15 Hz, at 23°C) as temperature increases. Phase transfer function slope decreases (from −70°/Hz at 8°C to −25°/Hz at 23°C) as temperature increases. Nonlinear properties of ERG at high input intensity are modelled by a quadratic nonlinearity, low-pass prefilter with cutoff above 12.4 Hz, and low-pass postfilter broadly peaked at 6–10 Hz with cutoff above 20 Hz. For low input intensity, ERG exhibits linear properties with low-pass filtering sharply cut off above 6 Hz.a-Wave and slow P-III were isolated by aspartate treatment; depolarizing bipolar cell activity was examined using ethanol/GABA treatment of retina. High-frequency components, including broad mid-frequency peak, were attenuated and low-frequency components were enhanced with aspartate. Transfer function narrows and peaks at a lower frequency with ethanol/GABA.