Higher Concentrations Of AgNPs Research Articles

Fabrication and Characterization of Silver Nanoparticle-Doped Chitosan/Carboxymethyl Cellulose Nanocomposites for Optoelectronic and Biological Applications.

The synthesis of nanoparticles using environmentally friendly methods for applications in fields such as food packaging and biomedicine has been gaining increasing attention. Organic-inorganic nanostructures offer opportunities to create innovative materials suitable for use in optoelectronics and biological applications. In this study, we focused on producing nanocomposite films by blending carboxymethyl cellulose (CMC) and chitosan (CS) polymers in equal proportions (50/50 wt %) and adding silver nanoparticles (Ag NPs) through a solution casting process. Our objective was to examine how the introduction of Ag NPs influenced the structural, optical, mechanical, electrical, and antibacterial properties of the virgin CMC/CS composites. XRD patterns of the prepared samples indicated the presence of crystalline Ag phases within the CMC/CS blend. FT-IR spectroscopy showed the primary vibrational peaks associated with CMC and CS, which exhibited reduced intensity after the addition of Ag NPs. The UV absorption of the nanocomposites exhibited a gradual increase and a shift toward longer wavelengths. The electrical properties are enhanced with higher concentrations of Ag NPs. An increase in the content of Ag NPs resulted in a corresponding enhancement of antibacterial activity against both Staphylococcus aureus and Escherichia coli. The CMC/CS-Ag-doped films demonstrated significant enhancements in Young's modulus (Y), tensile stress (σt), and elongation at break (εB). These findings suggest that these nanocomposite films hold promise for potential applications in optoelectronics and biological fields.

Effects of sliver nanoparticles on nitrogen removal by the heterotrophic nitrification-aerobic denitrification bacteria Zobellella sp. B307 and their toxicity mechanisms

Due to the widespread use of sliver nanoparticles (AgNPs), a large amount of AgNPs has inevitably been released into the environment, and there is growing concern about the toxicity of AgNPs to nitrogen-functional bacteria. In addition to traditional anaerobic denitrifying bacteria, heterotrophic nitrification-aerobic denitrification (HNAD) bacteria are also important participants in the nitrogen cycle. However, the mechanisms by which AgNPs influence HNAD bacteria have yet to be explicitly demonstrated. In this study, the inhibitory effects of different concentrations of AgNPs on a HNAD bacteria Zobellella sp. B307 were investigated, and the underlying mechanism was explored by analyzing the antioxidant system and the activities of key denitrifying enzymes. Results showed that AgNPs could inhibit the growth and the HNAD ability of Zobellella sp. B307. AgNPs could accumulate on the surface of bacterial cells and significantly destroyed the cell membrane integrity. Further studies demonstrated that the presence of high concentration of AgNPs could result in the overproduction of reactive oxygen species (ROS) and related oxidative stress in the cells. Furthermore, the catalytic activities of key denitrifying enzymes (nitrate reductase (NAR), nitrite reductase (NIR), and nitrous oxide reductase (N2OR)) were significantly suppressed under exposure to a high concentration of AgNPs (20 mg·L−1), which might be responsible for the inhibited nitrogen removal performance of strain B307. This work could improve our understanding of the inhibitory effect and underlying mechanism of AgNPs on HNAD bacteria.

Poly-3-hydroxybutyrate-silver nanoparticles membranes as advanced antibiofilm strategies for combatting peri-implantitis

Dental implant success is threatened by peri-implantitis, an inflammation leading to implant failure. Conventional treatments struggle with the intricate microbial and host factors involved. Antibacterial membranes, acting as barriers and delivering antimicrobials, may offer a promising solution. Thus, this study highlights the potential of developing antibacterial membranes of poly-3-hydroxybutyrate and silver nanoparticles (Ag Nps) to address peri-implantitis challenges, discussing design and efficacy against potential pathogens.Electrospun membranes composed of PHB microfibers and Ag Nps were synthesized in a blend of DMF/chloroform at three different concentrations. Various studies were conducted on the characterization and antimicrobial activity of the membranes. The synthesized Ag Nps ranged from 4 to 8 nm in size. Furthermore, Young's modulus decreased, reducing from 13.308 MPa in PHB membranes without Ag Nps to 0.983 MPa in PHB membranes containing higher concentrations of Ag Nps. This demonstrates that adding Ag Nps results in a less stiff membrane. An increase in elongation at break was noted with the rise in Ag Nps concentration, from 23.597 % in PHB membranes to 60.136 % in PHB membranes loaded with Ag Nps.The antibiotic and antibiofilm activity of the membranes were evaluated against Pseudomonas aeruginosa, Staphylococcus aureus, Streptococcus mutans, and Candida albicans. The results indicated that all PHB membranes containing Ag Nps exhibited potent antibacterial activity by inhibiting the growth of biofilms and planktonic bacteria. However, inhibition of C. albicans occurred only with the PHB-Ag Nps C membrane.These findings emphasize the versatility and potential of Ag Nps-incorporated membranes as a multifunctional approach for preventing and addressing microbial infections associated with peri-implantitis. The combination of antibacterial and antibiofilm properties in these membranes holds promise for improving the management and treatment of peri-implantitis-related complications.

Antifungal potential of commercial silver nanoparticles against rice blast pathogen Magnaporthe oryzae

The rice blast caused by Magnaporthe oryzae is one of the major and recurrent threats to sustainable rice production in Bangladesh. To mitigate this problem, the present study was undertaken with the aim to investigate the inhibitory effects of commercially available silver nanoparticles (Ag-NPs) on the growth and development of Magnaporthe oryzae, and control of rice blast disease. In vitro experiments demonstrated that Ag-NPs significantly suppressed the radial growth of fungal mycelium, with the highest inhibition (83.54%) at a concentration of 250 ppm, while Trooper 75 WP at 400 ppm completely inhibited the fungal mycelial growth. The production of conidia and colony formation were also reduced significantly by Ag-NPs treatment. Furthermore, Ag-NPs exhibited a substantial inhibitory effect on the germination of M. oryzae conidia, with complete inhibition observed at concentrations between 200 and 250 ppm. Comparative analyses revealed that the fungicidal effect of Ag-NPs was superior to silver nitrate (AgNO3), suggesting the augmented activity of the nano properties. The sensitivity of M. oryzae to Ag-NPs was determined by calculating EC50 and EC95 values, which indicated a mean EC50 value of 131.5 ppm and a mean EC95 value of 276 ppm. Additionally, in vivo experiments showed that preventive and curative treatments with Ag-NPs significantly reduced disease incidence and severity in rice plants infected with M. oryzae, with efficacy comparable to that of the fungicide Trooper 75 WP. At higher concentrations of Ag-NPs, preventive treatment was found to be more effective than curative treatment in disease control. These findings highlighted the potential of Ag-NPs as an effective alternative for the management of rice blast. Ann. Bangladesh Agric. (2023) 27 (1): 17-30

Assessment of possible potential toxicity risks in albino mice exposed to amine coated silver nanoparticles

Nanoparticles have a wide range of physiochemical properties that can harm many organs, tissues, enzymatic and hormonal activities. Throughout the world, silver nanoparticles (AgNPs) are frequently used in consumer products. The aim of the current study was to synthesize the AgNPs and to investigate their impact on hormones, antioxidant enzymatic activities, and histopathology of the liver of albino mice. The precursor salt silver nitrate and the reducing and capping agent triethylamine were used to synthesize silver nanoparticles. SEM analysis indicated particles ranged between 10–20 nm. The EDX analysis indicated distinct peaks, which illuminated the crystalline, spherical, and face-centred cubic structure of the silver particles being analyzed. Further, the albino model showed that increasing the concentration of AgNPs increased the level of plasma cortisol, T3 and T4. Catalase (CAT), Superoxide Dismutase (SOD), and Glutathione S-Transferase (GST) activities increased at 5 mg/kg but decreased dramatically at 50 mg/kg, whereas Glutathione (GSH) activities and lipid peroxidation (LPO) levels increased with increasing AgNPs concentration. Furthermore, AgNPs caused neutrophilic and lymphocytic infiltration, central vein damage, and hemorrhage in albino mouse liver at low concentrations (5 mg/kg). High concentrations of AgNPs (50 mg/kg) caused the formation of pknotic nuclei, pyknosis, apoptosis, and vacuolation. The study concluded that AgNPs have dose-dependent toxicity that alters stress hormones, T3 and T4, antioxidant enzymatic activities, and produces some histopathological alterations.

Effects of Silver Nanoparticles on Growth of E. coli: A Study Examining Various Antibiotic Factors

Nanomedicine, an emerging and revolutionary scientific field, warrants greater scientific investigation on the antibacterial applications of AgNPs; hence, this research serves to assess AgNPs’ antibacterial effectiveness on the growth of E. coli while establishing the antibiotic’s optimal conditions for drug delivery. The hypothesis expects that optimal drug delivery of AgNPs will occur at higher concentrations of AgNPs and neutral pHs. To demonstrate this, 1:10 serial AgNP dilutions were placed in E. coli cultures and were treated with the varying environmental factors of concentration and pHs. The results supported our hypotheses with the exception of our pH hypothesis, for the data showed that having a basic pH increased the efficiency of the nanoparticles for all concentrations, except for the 500 µg/L concentration. Nevertheless, higher concentrations of nanoparticles have the highest and fastest increases in their zones of inhibitions. The concentration and pH experiments yielded statistical significance. With nanomedicine’s ability to diagnose, detect, and deliver drugs, its research applications are endless, proving it has a significant impact in the fields of cancer drug delivery, E. coli toxicity, and ectopic pregnancies, and ultimately, having the potential to save over two million lives every year.

Biopolymeric nanofibrous scaffolds of poly(3-hydroxybuthyrate)/chitosan loaded with biogenic silver nanoparticle synthesized using curcumin and their antibacterial activities

The increasing prevalence of multi-drug resistant bacteria poses a significant threat to public health, especially in wound infections. Developing new bactericidal agents and treatment strategies is crucial to address this issue. In this study, biopolymeric nanofibrous scaffolds containing green-synthesized silver nanoparticles (AgNPs) with curcumin (CUR) were evaluated as antimicrobial materials for wound healing therapy. Firstly, CUR was utilized to synthesize AgNPs, which were then analyzed using various analytical methods. The microstructural analysis revealed that the biogenic AgNPs, which had a spherical shape and an average size of 19.83 nm, were uniformly anchored on PHB/CTS nanofibers. Then, the AgNPs with various content (0.25–1%wt) were incorporated into PHB/CTS matrix to enhance its wettability, thermal and bactericidal behaviors. The nanofibrous scaffolds were characterized by FT-IR, FE-SEM, TGA analysis and water contact angle measurement. Overall, the addition of CUR-AgNPs to the PHB/CTS matrix led to a reduction in fiber diameter, enhanced hydrophilicity and improved thermal properties. Additionally, antibacterial activity against Staphylococcus aureus and Escherichia coli was performed on samples of AgNPS and PHB/CTS/CUR-Ag. The synthesized AgNPs showed antibacterial activity against both microorganisms, especially against S. aureus. Higher concentrations of AgNPs in nanofibers led to a significant reduction in bacterial colony formation. The results displayed that PHB/CTS/CUR-AgNPs nanofibrous scaffolds could be a promising material for the biomedical applications such as wound healing.

Surface-Enhanced Raman Scattering (SERS) Substrates Based on Ag-Nanoparticles and Ag-Nanoparticles/Poly (methyl methacrylate) Composites.

SERS substrates formed by spherical silver nanoparticles (Ag-NPs) with a 15 nm average diameter adsorbed on Si substrate at three different concentrations and Ag/PMMA composites formed by an opal of PMMA microspheres of 298 nm average diameter were synthesized. The Ag-NPs were varied at three different concentrations. We have observed from SEM micrographs, in the Ag/PMMA composites, the periodicity of the PMMA opals is slightly altered as the Ag-NP concentration is increased; as a consequence of this effect, the PBGs maxima shift toward longer wavelengths, decrease in intensity, and broaden as the Ag-NP concentration is increased in the composites. The performance of single Ag-NP and Ag/PMMA composites as SERS substrates was determined using methylene blue (MB) as a probe molecule with concentrations in the range of 0.5 µM to 2.5 µM. We found that in both single Ag-NP and Ag/PMMA composites as SERS substrates, the enhancement factor (EF) increases as the Ag-NP concentration is increased. We highlight that the SERS substrate with the highest concentration of Ag-NPs has the highest EF due to the formation of metallic clusters on the surface, which generates more "hot spots". The comparison of the EFs of the single Ag-NP with those of Ag/PMMA composite SERS substrates shows that the EFs of the former are nearly 10-fold higher than those of Ag/PMMA composites. This result is obtained probably due to the porosity of the PMMA microspheres that decreases the local electric field strength. Furthermore, PMMA exerts a shielding effect that affects the optical efficiency of Ag-NPs. Moreover, the metal-dielectric surface interaction contributes to the decrease in the EF. Other aspect to consider in our results is in relation to the difference in the EF of the Ag/PMMA composite and Ag-NP SERS substrates and is due to the existing mismatch between the frequency range of the PMMA opal stop band and the LSPR frequency range of the Ag metal nanoparticles adsorbed on the PMMA opal host matrix.

Effect of Biosynthesized Silver Nanoparticles on the Growth of the Green Microalga Haematococcus pluvialis and Astaxanthin Synthesis.

Silver nanoparticles (AgNPs) are widely known for their antimicrobial activity in various systems from microorganisms to cell cultures. However, the data on their effects on microalgae are very limited. Unicellular green algae Haematococcus pluvialis is known for its ability to accumulate large amounts of astaxanthin under stress conditions. Therefore, it can be used as a suitable model system to test the influence of AgNPs on stress induction in unicellular algae, with visible phenotypic effects, such as astaxanthin synthesis and cell morphology. This study tested different AgNP concentrations (0-8 mg/L) effects on different growth stages (red and green) of H. pluvialis culture. Effects on cell morphology, culture productivity, and astaxanthin synthesis were evaluated. Data showed that the addition of high concentrations of AgNPs to the growing culture had a significant negative impact on culture productivity. Green-stage (HpG) cultures productivity was reduced by up to 85% by increasing AgNPs concentration to 8 mg/L while the impact on red-stage (HpR) culture was lower. Astaxanthin concentration measurements showed that AgNPs do not have any effect on astaxanthin concentration in HpG culture and caused decreased astaxanthin production rate in HpR culture. HpG culture astaxanthin concentration stayed constant at ~0.43% dry weight, while HpR culture astaxanthin concentration was significantly reduced from 1.89% to 0.60% dry weight by increasing AgNP concentration. AgNPs in the media lead to significant changes in cell morphology in both HpG and HpR cultures. Cell deformations and disrupted cytokinesis, as well as AgNPs and induced sexual reproduction, were observed.

Phytotoxicity and Antimicrobial Activity of Green Synthesized Silver Nanoparticles Using Nigella sativa Seeds on Wheat Seedlings

Recently, the green synthesis of nanomaterials has grown in popularity and has become one of the most used approaches. Plant extracts are safe for the environment and could be cost-effective for nanoparticle preparation. Silver nanoparticles (AgNPs) have been synthesized using aqueous extracts of Nigella sativa (N. sativa) seeds. The formation of AgNPs was confirmed by using an X-ray diffractometer, a UV-visible spectrometer, and a transmission electron microscope. The phytotoxicity and genotoxicity of different AgNP concentrations (12.5, 25, 50, 75, and 100 μg·L−1) were evaluated by wheat (Triticum aestivum L.) seed germination. The results showed that AgNPs did not significantly affect germination, while root and coleoptile lengths decreased considerably. On the contrary, the biomass of seedlings markedly increased in response to AgNP treatments. Moreover, genotoxicity was detected, especially at high concentrations of AgNPs. DNA, RNA, and total soluble proteins of wheat seedlings significantly decreased. In addition, antimicrobial activities of biosynthesized AgNPs were detected.

Green Synthesis of Silver Nanoparticles Using Randia aculeata L. Cell Culture Extracts, Characterization, and Evaluation of Antibacterial and Antiproliferative Activity.

The demand for metallic nanoparticles synthesized using green methods has increased due to their various therapeutic and clinical applications, and plant biotechnology may be a potential resource facilitating sustainable methods of AgNPs synthesis. In this study, we evaluate the capacity of extracts from Randia aculeata cell suspension culture (CSC) in the synthesis of AgNPs at different pH values, and their activity against pathogenic bacteria and cancer cells was evaluated. Using aqueous CSC extracts, AgNPs were synthesized with 10% (w/v) of fresh biomass and AgNO3 (1 mM) at a ratio of 1:1 for 24 h of incubation and constant agitation. UV-vis analysis showed a high concentration of AgNPs as the pH increased, and TEM analysis showed polydisperse nanoparticles with sizes from 10 to 90 nm. Moreover, CSC extracts produce reducing agents such as phenolic compounds (162.2 ± 27.9 mg gallic acid equivalent/100 g biomass) and flavonoids (122.07 ± 8.2 mg quercetin equivalent/100 g biomass). Notably, AgNPs had strong activity against E. coli, S. pyogenes, P. aeruginosa, S. aureus, and S. typhimurium, mainly with AgNPs at pH 6 (MIC: 1.6 to 3.9 µg/mL). AgNPs at pH 6 and 10 had a high antiproliferative effect on cancer cells (IC50 < 5.7 µg/mL). Therefore, the use of cell suspension cultures may be a sustainable option for the green synthesis of AgNPs.

Synthesis and Evaluation of Quorum Quenching activity of Ag, TiO2 and ZnO metal nanoparticles against wild type and mutant strains of Chromobacterium violaceum

The conventional strategies to combat pathogenic microbes seem to be inefficient to curb the menace of pathogens. The bacterial biofilm is a product of the quorum sensing (QS) process mediated by acylhomoserine lactones (AHL) in many pathogens. The QS inhibitors or quorum quenching (QQ) molecules that disrupt bacterial communication offer an alternative promising strategy to lower antibiotic resistance in pathogens. The nanoparticles can exhibit QQ potential that interrupts cell-to-cell communication. In the present study, laboratory synthesized metal-based nanoparticles such as silver (Ag), titanium oxide(TiO2) and zinc oxide (ZnO) were characterized using powder X-Ray diffractometer, UV-Visible spectroscopy, EDS, Cyclic voltammetry and TEM imaging technique. Ag and TiO2 nanoparticles were synthesized by the green synthesis method in the form of quantum dots, which was evidenced from TEM images on a less than 7nm scale. Silver quantum dots with size variation from 2.4nm to 4.2nm and TiO2 with size ranging from 4.9nm to 8.5nm were detected. The synthesized nanoparticles were elucidated for their potential as QQ by employing both wild and mutant strains of Chromobacterium violaceum. Nanoparticles affected violacein pigment production in both the strains of C. violaceum. Lower concentrations of NPs did not show any considerable effect on the AHLdependent Quorum sensing system, however, at moderate to higher concentrations, metal NPs exhibit Quorum Q activity. Higher concentrations of AgNPs and ZnONPs (i.e. 250 ppm and above) show cell inhibitory activity. In the percent pigment(violacein) inhibition assay, maximum pigment inhibition was detected at a concentration range of 100-125ppm in both the strains of C. violaceum without inhibiting cell growth. In the case of TiO2NPs, maximum pigment inhibition was detected at 500ppm without affecting cell growth in both the strains of C. violaceum. The percent pigment inhibition observed in all nanoparticles was nearly similar in both strains of C. violaceum indicating that the mechanism of action of pigment inhibition is due to interference at the reception of the AHL signaling molecule.

Characterization and Evaluation of Antimicrobial Potential of Trigonella incise (Linn) Mediated Biosynthesized Silver Nanoparticles.

The goal of the research was to explore a new green method used to synthesize silver nanoparticles (Ag NPs) from an aqueous extract of Trigonella incise, which serves as a reducing and stabilizing agent. The obtained results showed an 85% yield of nanoparticles by using 2:5 (v/v) of 5% plant extract with a 0.5 M solution of AgNO3. Different techniques were used to characterize the synthesized Ag NPs, including X-ray diffraction (XRD), Fourier-transform infrared spectroscopy (FT-IR), scanning electron microscopy (SEM), and UV–visible spectroscopy. The UV–visible spectra of green synthesized silver nanoparticles showed maximum absorption at a wavelength of 440 nm. The FT-IR studies revealed the stretching oscillation frequency of synthesized silver nanoparticles in the absorption band near 860 cm−1. Similarly, the bending and stretching oscillation frequencies of the NH function group were assigned to the band in the 3226 cm−1 and 1647 cm−1 regions. The bending vibration of C-O at 1159 cm−1 confirmed the carbonyl functional group that was also assigned to the small intensity band in the range of 2361 cm−1. The X-ray diffraction analysis of Ag NPs revealed four distinct diffraction peaks at 2θ of 38°, 45°, 65° and 78°, corresponds to (111), (200), (220) and (311) of the face-centered cubic shape. The round shape morphology of Ag NPs with a mean diameter in the range 20–80 nm was analyzed via SEM images. Furthermore, the nanoparticles showed more significant antimicrobial activity against Salmonella typhi (S. typhi) and Staphylococcus aureus (S. aureus) with an inhibition zone of 21.5 mm and 20.5 mm at 6 μg/mL concentrations, respectively, once compared to the standard reference. At concentrations of 2 µg/mL and 4 µg/mL, all of the bacterial strains showed moderate activity, with inhibition zones ranging from 11 mm to 18.5 mm. Even at high concentrations of AgNPs, S. typhi showed maximum resistance. The best antifungal activity was observed by synthesized Ag NPs against Candida albicans (C. albicans) with 21 mm zone of inhibition, as compared to a standard drug which gives 22 mm of inhibition. Therefore, we conclude that the antibacterial and antifungal activities showed satisfactory results from the synthesized Ag NPs.

Green nanopriming: responses of alfalfa (Medicago sativa L.) seedlings to alfalfa extracts capped and light-induced silver nanoparticles

The application of nanotechnology in agriculture can remarkably improve the cultivation and growth of crop plants. Many studies showed that nanoparticles (NPs) made plants grow more vigorously. Light can make NPs aggregated, leading to the reduction of the NPs toxicity. In addition, treatment with NPs had a “hormesis effect” on plants. In this study, light-induced silver nanoparticles (AgNPs) were synthesized by using the alfalfa (Medicago sativa L.) extracts, and then the optimal synthetic condition was determined. Light-induced AgNPs were aggregated, spherical and pink, and they were coated with esters, phenols, acids, terpenes, amino acids and sugars, which were the compositions of alfalfa extracts. The concentration of free Ag+ was less than 2 % of the AgNPs concentration. Through nanopriming, Ag+ got into the seedlings and caused the impact of AgNPs on alfalfa. Compared with the control group, low concentration of light-induced AgNPs had a positive effect on the photosynthesis. It was also harmless to the leaf cells, and there was no elongation effect on shoots. Although high concentration of AgNPs was especially beneficial to root elongation, it had a slight toxic effect on seedlings due to the accumulation of silver. With the increase of AgNPs concentration, the content of silver in the seedlings increased and the silver enriched in plants was at the mg/kg level. Just as available research reported the toxicity of NPs can be reduced by using suitable synthesis and application methods, the present light induction, active material encapsulation and nanopriming minimized the toxicity of AgNPs to plants, enhancing the antioxidant enzyme system.Graphical

Optimized Synthesis of Small and Stable Silver Nanoparticles Using Intracellular and Extracellular Components of Fungi: An Alternative for Bacterial Inhibition.

Silver nanoparticles (AgNPs) represent an excellent option to solve microbial resistance problems to traditionally used antibiotics. In this work, we report optimized protocols for the production of AgNPs using extracts and supernatants of Trichoderma harzianum and Ganoderma sessile. AgNPs were characterized using UV-Vis spectroscopy and transmission electron microscopy, and the hydrodynamic diameter and Z potential were also determined. The obtained AgNPs were slightly larger using the fungal extract, and in all cases, a quasi-spherical shape was obtained. The mean sizes of AgNPs were 9.6 and 19.1 nm for T. harzianum and 5.4 and 8.9 nm for G. sessile using supernatant and extract, respectively. The AgNPs were evaluated to determine their in vitro antibacterial effect against Escherichia coli, Pseudomonas aeruginosa and Staphylococcus aureus. The minimum inhibitory concentration (MIC) was determined, and in all cases the AgNPs showed an antimicrobial effect, with a MIC varying from 1.26–5.0 µg/mL, depending on the bacterial strain and type of nanoparticle used. Cytotoxicity analyses of AgNPs were carried out using macrophages and fibroblast cell lines. It was determined that the cell viability of fibroblasts exposed for 24 h to different concentrations of AgNPs was more than 50%, even at concentrations of up to 20 µg/mL of silver. However, macrophages were more susceptible to exposure at higher concentrations of AgNPs as their viability decreased at concentrations of 10 µg/mL. The results presented here demonstrate that small AgNPs are obtained using either supernatants or extracts of both fungal strains. A remarkable result is that very low concentrations of AgNPs were necessary for bacterial inhibition. Furthermore, AgNPs were stable for more than a year, preserving their antibacterial properties. Therefore, the reported optimized protocol using fungal supernatants or extracts may be used as a fast method for synthesizing small AgNPs with high potential to use in the clinic.

Biotechnological use of the ubiquitous fungus Penicillium sp. 8L2: Biosorption of Ag(I) and synthesis of silver nanoparticles

In this work, the efficiency of the ubiquitous fungus Penicillium sp. 8L2 to remove Ag(I) ions from synthetic solutions and its potential to synthesize silver nanoparticles (AgNPs) was evaluated. Using a Rotatable Central Composite Design pH and biomass concentration were optimized. Maximum biosorption capacity 51.53 mg/g, by Langmuir model, comparing favourably with other reports. The fungal biomass was characterized by Fourier Transform Infrared Spectroscopy (FT-IR) and analyzed before and after the biosorption process by different techniques: X-ray diffraction (XRD), Field Emission Scanning Electron Microscopy (FE-SEM), Ultra-High Resolution Transmission Electron Microscopy and Energy Dispersive X-ray (HR-TEM-EDX) and Ultraviolet–Visible Spectrophotometry (UV–vis). The results showed that the fungus applied several mechanisms to remove Ag(I) ions from the solution and that some of them induced the synthesis of AgNPs. This fact could be verified in the synthesis tests from the cell extract in which aqueous suspensions with high concentrations of AgNPs were obtained. These nanoparticles had diameters between 2 and 9 nm and therefore a high potential for their use as biocidal agents. The results indicated that the synthesis of nanoparticles could be an added value to the heavy metal biosorption process.

STABILITY AND ANTIBACTERIAL ACTIVITY TEST OF NANOSILVER BIOSYNTHETIC HYDROGEL

Objective: This study aims to formulate nanosliver (AgNPs) biosynthetic hydrogel for topical antibacterial treatment and its stability and antibacterial activity. Methods: The mixture (Silver nitrate solution and Turmeric juice) was stirred at room temperature for 24 h; afterward, it was analyzed using UV-VIS spectrophotometry, particle size analysis, and TEM. The carbopol 940 was selected as a gelling agent with an AgNPs concentration of 5%, 10%, 20%, and 30%. Furthermore, the gel preparation was tested for stability using the cycling test method and antibacterial activity. The antibacterial activity against Staphylococcus aureus, Staphylococcus epidermidis, Escherichia coli, and Pseudomonas aeruginosa. Results: The nanosilver biosynthetic has a yellow-brown color with the maximum wavelength peak at 433 nm, and a particle size of 157.4-166.7 nm. TEM analysis showed that AgNPs have a round shape, while the antibacterial activity of hydrogel preparations was moderately inhibited. Furthermore, the hydrogel was evaluated for pH, viscosity, dispersibility, and antibacterial activity before-after the cycling test. Formula with 30% AgNPs is chosen formula with pH value of 5.87±0.65; viscosity of 4833.3±2.82 c. Ps; and dispersibility of 5.50±0.15 after cycling test. Conclusion: The high concentration of AgNPs will increase the viscosity, pH, and dispersibility. Formula with 30% AgNPs have the highest antibacterial activity. Furthermore, all hydrogel preparations meet the requirements of Indonesian Standard Product (SNI) No. 06-2588-1992 for good gel stability before and after the cycling test.

Genetic Relatedness, Antibiotic Resistance, and Effect of Silver Nanoparticle on Biofilm Formation by Clostridium perfringens Isolated from Chickens, Pigeons, Camels, and Human Consumers.

In this study, we determined the prevalence and toxin types of antibiotic-resistant Clostridium perfringens in chicken, pigeons, camels, and humans. We investigated the inhibitory effects of AgNPs on biofilm formation ability of the isolates and the genetic relatedness of the isolates from various sources determined using RAPD-PCR. Fifty isolates were identified using PCR, and all the isolates were of type A. The cpe and cpb2 genes were detected in 12% and 56% of the isolates, respectively. The effect of AgNPs on biofilm production of six representative isolates indicated that at the highest concentration of AgNPs (100 µg/mL), the inhibition percentages were 80.8–82.8%. The RAPD-PCR patterns of the 50 C. perfringens isolates from various sources revealed 33 profiles and four clusters, and the discriminatory power of RAPD-PCR was high. Multidrug-resistant C. perfringens isolates are predominant in the study area. The inhibition of biofilm formation by C. perfringens isolates was dose-dependent, and RAPD-PCR is a promising method for studying the genetic relatedness between the isolates from various sources. This is the first report of AgNPs’ anti-biofilm activity against C. perfringens from chickens, pigeons, camels, and humans, to the best of our knowledge.

Phytofabricated Silver Nanoparticles (AgNPs) Applied in Vase Solution as a Novel Anti-Microbial Agent for Enhancing the Vase-life of Cut-Rose Flower

Plant extract has been exploited for biogenic synthesis of metallic nanoparticles. This is considered as the promising alternative routes of chemical and physical synthesis methods owing to their abundancy in nature and ecofriendly friendly synthesis protocol. Rose is the top-ranked and universally favorite cut flower. Poor post-harvest management deteriorates the quality and reduces the vase life. Microbial proliferation of the stem base in the vase solution is a concern for these cut flowers that shortens their vase-life. To overcome this problem, a smart solution i.e. phytofabricated silver nanoparticles have been shown to act as anti-microbial agent. In this study, leaf extracts of Camellia sinensis were exploited to fabricate biogenic silver nanoparticles which showing UV-peak absorption ranging from 412-500 nm. This biogenic silver nanoparticles were applied @ 0.01, 0.05 and 0.1mM and compared with a control (without AgNPs) and silver nitrate (AgNO3). Interestingly, AgNPs showed a strong antimicrobial activity in vase solution and cut roses extended their vase life up to 13 days compared to 8 days in control and 9 days in AgNO3. Statistical differences in flower opening, bacterial growth (CFUmL−1) in vase solutions, water uptake, relative fresh weight and vase life of cut roses were found among treatments. In vitro microbial analysis and microscopic investigation of vessels showed that the development of microorganisms was reduced by a high concentration of AgNPs (0.1mM) at the cut end of flower and improving water uptake that followed by extended flowers vase life. The unique phytofabricated AgNPs technology can serve as a promising preservative to increase the ornamental value of cut rose flowers. Taken all together, applied phytofabricated AgNPs in vase solution significantly enhance vase life of cut rose flowers over control.

Anti-Tumor Activity of Eco-Friendly AgNPs Derived from Musa Paradisiaca Pseudo Stem Methanolic Extracts and their Antibacterial and Antioxidant Activities in Ovarian Cancer Cell Line-PA1

Abstract: Musa Paradisiaca, commonly known as Banana, is a gigantic herb. Its main upright stem is called as Pseudostem. Banana plant have lots of medicinal uses.This piece of work describes the anti-cancerous activity of methanolic extracts made from pseudo stem of Musa paradisiaca. Anti-tumour activity of biogenic AgNPs has not been digged in the field of Ovarian cancer. The synthesized silver nanoparticles were identified by the formation of light-yellow colour solution and U.V-Visible spectrophotometer analysis which showed maximum absorbance at 423nm. The presence of ketones, methyl groups, nitrosamines and aromatic rings as functional groups in AgNPS was identified using FTIR. The antibacterial studies were performed by Agar Diffusion method against different strains of bacteria. The AgNPs showed antioxidant activity through DPPH assay. The antiproliferative activity of AgNPs was demonstrated against ovarian cancer cell line Pa 1 with MTT assay and confirmed using PI staining. In the toxicity study, a significant mortality rate was observed with an IC50 concentration of 250 µg, so they are cytotoxic at high concentrations of AgNPs. Keywords: Biosynthesis; Silver nanoparticles; Characterization; Antibacterial; Antioxidant; Anticancer; Flowcytometry