Anti-Tumor Activity of Eco-Friendly AgNPs Derived from Musa Paradisiaca Pseudo Stem Methanolic Extracts and their Antibacterial and Antioxidant Activities in Ovarian Cancer Cell Line-PA1

Abstract

Abstract: Musa Paradisiaca, commonly known as Banana, is a gigantic herb. Its main upright stem is called as Pseudostem. Banana plant have lots of medicinal uses.This piece of work describes the anti-cancerous activity of methanolic extracts made from pseudo stem of Musa paradisiaca. Anti-tumour activity of biogenic AgNPs has not been digged in the field of Ovarian cancer. The synthesized silver nanoparticles were identified by the formation of light-yellow colour solution and U.V-Visible spectrophotometer analysis which showed maximum absorbance at 423nm. The presence of ketones, methyl groups, nitrosamines and aromatic rings as functional groups in AgNPS was identified using FTIR. The antibacterial studies were performed by Agar Diffusion method against different strains of bacteria. The AgNPs showed antioxidant activity through DPPH assay. The antiproliferative activity of AgNPs was demonstrated against ovarian cancer cell line Pa 1 with MTT assay and confirmed using PI staining. In the toxicity study, a significant mortality rate was observed with an IC50 concentration of 250 µg, so they are cytotoxic at high concentrations of AgNPs. Keywords: Biosynthesis; Silver nanoparticles; Characterization; Antibacterial; Antioxidant; Anticancer; Flowcytometry