Higher Species Abundance Research Articles

Unlocking Double Redox Reaction of Metal–Organic Framework for Aqueous Zinc‐Ion Battery

AbstractMetal–organic frameworks (MOFs) show wide application as the cathode of aqueous zinc‐ion batteries (AZIBs) in the future owning to their high porosity, diverse structures, abundant species, and controllable morphology. However, the low energy density and poor cycling stability hinder the feasibility in practical application. Herein, an innovative strategy of organic/inorganic double electroactive sites is proposed and demonstrated to obtain extra capacity and enhance the energy density in a manganese‐based metal–organic framework (Mn‐MOF‐74). Simultaneously, its energy storage mechanism is systematically investigated. Moreover, profiting from the coordination effect, the Mn‐MOF‐74 features with stable structure in ZnSO4 electrolyte. Therefore, the Zn/Mn‐MOF‐74 batteries exhibit a high energy density and superior cycling stability. This work aids in the future development of MOFs in AZIBs.

Unlocking Double Redox Reaction of Metal-Organic Framework for Aqueous Zinc-Ion Battery.

Metal-organic frameworks (MOFs) show wide application as the cathode of aqueous zinc-ion batteries (AZIBs) in the future owning to their high porosity, diverse structures, abundant species, and controllable morphology. However, the low energy density and poor cycling stability hinder the feasibility in practical application. Herein, an innovative strategy of organic/inorganic double electroactive sites is proposed and demonstrated to obtain extra capacity and enhance the energy density in a manganese-based metal-organic framework (Mn-MOF-74). Simultaneously, its energy storage mechanism is systematically investigated. Moreover, profiting from the coordination effect, the Mn-MOF-74 features with stable structure in ZnSO4 electrolyte. Therefore, the Zn/Mn-MOF-74 batteries exhibit a high energy density and superior cycling stability. This work aids in the future development of MOFs in AZIBs.

Abstract MP01: Life-Course Socioeconomic Status and the Gut Microbiome in the Hispanic Community Health Study/Study of Latinos (HCHS/SOL)

Introduction: Socioeconomic status (SES) in childhood and beyond may influence the gut microbiome (GMB), with implications for disease risk. Studies evaluating the relationship between life-course SES and the gut microbiome (GMB) are sparse, particularly among Hispanic/Latino individuals, who have a high prevalence of low SES. Hypothesis: Low life-course SES indicators are associated with less diversity and overall composition of the gut microbiome in United States (U.S.) Hispanic/Latino adults. Methods: The Hispanic Community Health Study/Study of Latinos (HCHS/SOL) is a multi-site population-based cohort study conducted in the U.S. Childhood sanitation conditions (plumbing, sewer/septic tank), childhood SES (parental education and childhood hardship), adulthood neighborhood SES, and adulthood SES (education, income, and economic hardship) were used as life-course SES indicators. Shotgun sequencing was performed on stool samples (n=1057). Alpha-diversity was measured by the Shannon diversity index. Permutational multivariate analysis of variance was used to assess the association of SES indicators with overall microbiome composition, as measured using the Jensen-Shannon Divergence distance. Analysis of Compositions of Microbiomes was used to identify associations of life-course SES indicators with GMB species, adjusting for age, sex, BMI, U.S. or foreign-born, study center, Hispanic/Latino background, type of stool, diet quality, smoking, alcohol consumption, depressive and anxiety symptoms, diabetes, hypertension, cardiovascular disease, dyslipidemia, and antibiotic use. Results: Low sanitation and low SES during childhood were reported by 32% (342 of 1053) and 37% (330 of 884) of participants, respectively. In adulthood, 56% (585 of 1053) lived in a low SES neighborhood and 45% (447 of 1002) had low SES. Life-course SES indicators were not associated with GMB diversity. However, overall microbiome composition differed significantly according to childhood SES (R2 =0.28%, P = 0.04). Low childhood sanitation was associated with higher abundance of Ruminococcus torques and Clostridium lactatifermentans , and depleted abundance of Prevotella species. Low childhood SES was associated with higher abundance of species from genus Streptococcus (S. salivarius, S. parasanguinis, and S. infantis), Veillonella parvula, Prevotella stercorea, and Roseburia intestinalis. Low adulthood SES was associated with depleted abundance of species from Bacteroides, and living in a low adulthood neighborhood SES was associated with depleted abundance of species from Rikenella . Conclusion: Low childhood SES was associated with an altered GMB composition in U.S. Hispanic/Latino adults. Early-life SES may have long-term effects on GMB composition, underscoring another biological mechanism linking early childhood factors to adulthood disease.

Soil bacterial community in a photovoltaic system adopted different survival strategies to cope with small-scale light stress under different vegetation restoration modes.

Solar photovoltaic (PV) power generation is a major carbon reduction technology that is rapidly developing worldwide. However, the impact of PV plant construction on subsurface microecosystems is currently understudied. We conducted a systematic investigation into the effects of small-scale light stress caused by shading of PV panels and sampling depth on the composition, diversity, survival strategy, and key driving factors of soil bacterial communities (SBCs) under two vegetation restoration modes, i.e., Euryops pectinatus (EP) and Loropetalum chinense var. rubrum (LC). The study revealed that light stress had a greater impact on rare species with relative abundances below 0.01% than on high-abundance species, regardless of the vegetation restoration pattern. Additionally, PV shadowing increased SBCs' biomass by 20-30% but had varying negative effects on the numbers of Operational Taxonomic Unit (OTU), Shannon diversity, abundance-based coverage estimator (ACE), and Chao1 richness index. Co-occurrence and correlation network analysis revealed that symbiotic relationships dominated the key SBCs in the LC sample plots, with Chloroflexi and Actinobacteriota being the most ecologically important. In contrast, competitive relationships were significantly increased in the EP sample plots, with Actinobacteriota having the most ecological importance. In the EP sample plot, SBCs were found to be more tightly linked and had more stable ecological networks. This suggests that EP is more conducive to the stability and health of underground ecosystems in vulnerable areas when compared with LC. These findings offer new insights into the effects of small-scale light stress on subsurface microorganisms under different vegetation restoration patterns. Moreover, they may provide a reference for optimizing ecological restoration patterns in fragile areas.

Diversity of Culicoides in the middle belt of Ghana with Implications on the transmission of Mansonella perstans; a molecular approach

BackgroundCulicoides, also known as biting midges, carry pathogens which include Mansonella perstans. Mansonella perstans is a nematode parasite implicated in a number of disease outcomes. Even though a high prevalence of about 75% M. perstans infection has been recorded in some communities in the middle belt of Ghana, and a wide diversity of Culicoides species has been identified, the exact Culicoides species transmitting M. perstans in Ghana has not yet been deciphered. This study therefore aimed at assessing the species diversity of Culicoides and their role in the transmission of M. perstans in the middle belt of Ghana.MethodsCulicoides species were sampled from 11 communities in the Asante-Akim North and Sene West districts in the middle belt of Ghana. Centre for Disease Control (CDC) UV light traps, as well as human bait (i.e. human landing catch and engorged catch) methods were used to assess the species abundance and diversity of Culicoides in the study communities in the wet and dry season. A colorimetric Loop-Mediated Isothermal Amplification (LAMP) assay was performed to assess the vector competence of the various Culicoides species.ResultsA total of 4810 Culicoides from 6 species were sampled. These included Culicoides inornatipennis, C. milnei, C. schultzei, C. grahamii, C. neavei, and C. imicola. Culicoides imicola was the most abundant species (56%) followed by C. grahamii (16%). Light traps sampled the most diverse species (6 species). Human landing catch and engorged catch methods identified three anthropophilic species, C. grahamii, C. milnei, and C. inornatipennis, with C. grahamii being the most anthropophilic with a peak biting time between the hours of 5 p.m. to 6 p.m. Generally, there was relatively higher species abundance in the wet than dry season. LAMP assay identified C. grahamii as the potential vector for M. perstans transmission in the middle belt of Ghana.ConclusionsFor the first time, we have demonstrated that C. grahamii is the potential competent vector for M. perstans transmission in the middle belt of Ghana. It is more abundant in the rainy season and has a peak biting time between the hours of 5 and 6 p.m.Graphical

A global survey of prokaryotic genomes reveals the eco-evolutionary pressures driving horizontal gene transfer

Horizontal gene transfer, the exchange of genetic material through means other than reproduction, is a fundamental force in prokaryotic genome evolution. Genomic persistence of horizontally transferred genes has been shown to be influenced by both ecological and evolutionary factors. However, there is limited availability of ecological information about species other than the habitats from which they were isolated, which has prevented a deeper exploration of ecological contributions to horizontal gene transfer. Here we focus on transfers detected through comparison of individual gene trees to the species tree, assessing the distribution of gene-exchanging prokaryotes across over a million environmental sequencing samples. By analysing detected horizontal gene transfer events, we show distinct functional profiles for recent versus old events. Although most genes transferred are part of the accessory genome, genes transferred earlier in evolution tend to be more ubiquitous within present-day species. We find that co-occurring, interacting and high-abundance species tend to exchange more genes. Finally, we show that host-associated specialist species are most likely to exchange genes with other host-associated specialist species, whereas species found across different habitats have similar gene exchange rates irrespective of their preferred habitat. Our study covers an unprecedented scale of integrated horizontal gene transfer and environmental information, highlighting broad eco-evolutionary trends.

Exploring the Influence of Slope Gradient on Tree Species Richness and Forest Structure

Slope gradient is a critical environment factor influencing the abundance, distribution and diversity of vegetation. The objective of this study was to exploring the influence of slope gradient on tree species richness and forest structure in Kpatawee forest, Liberia. Tree diameter was measured at breast height for species more than 5 cm in diameter and over 3 m in height. Tree species diversity was analyzed using Shannon diversity index (Hˈ). Basal area (BA) which is the cross-sectional area of tree stems was measured through the diameter at breast height that is 1.3 m above ground level. Results showed that a total of 26 tree species were identified from the total sampling area (0.36 ha). The three richest families: Moraceae, Rutaceae and Euphorbiaceae appear to be well represented in the Kpatawee forest. These families become outnumbered in the herbaceous except for the Rutaceae family, which is ranked second. This is mainly due to their adaptation potential to wider agro-ecologies. Tree species richness was higher in the lower altitude. High species abundance was recorded in the lower altitude followed by the middle and upper. The lowest Shannon diversity index value (H' = 1.85) and the highest value (H' = 2.28) were recorded in the upper, and the lower altitudes, respectively. Despite their values, the biodiversity did not vary greatly from altitude to altitude. Diameter class distribution showed that more species were in the lower diameter classes and decreased gradually towards the higher classes. It is recommended that further studies on the entire Kpatawee forest is needed to fully understand the species richness in the forest.

Main drivers of dragonflies and damselflies (Insecta; Odonata) metacommunities in streams inside protected areas in the Brazilian Amazon.

The evaluation of environmental and spatial influence in freshwater systems is crucial for the conservation of aquatic diversity. So, we evaluated communities of Odonata in streams inside and outside sustainable use areas in the Brazilian western Amazon. We predicted that these streams would differ regarding habitat integrity and species α and β diversity. We also predict that environmental and spatial variables will be important for both suborders, but with more substantial effects on Zygoptera species, considering their nature of forest-specialist. The study was conducted in 35 streams, 19 inside and 16 outside sustainable use areas. The streams outside presented high species richness, abundance, and number of exclusive forest-specialist species from Zygoptera and higher scores of habitat integrity. In contrast, one sustainable use area presented the lowest values of these metrics. Besides, we found that environmental and spatial variables were significantly associated to Zygoptera species composition, but not with Anisoptera, which can be explained by their cosmopolitan nature. Our results indicated that an interplay between environmental and spatial processes determines the structure of the metacommunities of Zygoptera. The less effective dispersal rates and narrow ecological tolerance of Zygoptera species make them more influenced by local conditions and dispersal limitation, and more sensible to habitat modifications. We highlight the importance of improving the local management of the sustainable use areas by environmental agencies, mainly on areas that are losing their capacity to maintain the aquatic fauna, and implementation of social policies toward traditional people.

Dung Beetle Functional Diversity and Ecosystem Services (Resource Relocation) in Different Land Use Types in the Upper Walawe Basin Area

Conversion of forest habitat to different land uses will change the environmental quality of the altered land. Since the relationship between anthropogenic disturbance and animal diversity and ecosystem services emerged, much scientific research has been conducted on this aspect. The present study focuses on comparing dung beetles, a known biological indicator, on examining five diverse environs (large forests, forest patches, Pinus plantations (Pinus caribaea), home gardens, and tea plantations) Coprophagous beetles of the subfamily Scarabaeinae (Coleoptera: Scarabaeidae). This study examined whether dung beetles' diversity, species richness, functional groups, and ecosystem services (dung removal) vary with the habitat types. We also assumed that the intensity of anthropogenic activities varies in these habitats. The experiment was conducted for 48 hours each in five habitat types with three selected replicates per each habitat type. The Shannon-Weiner Diversity Index shows that the highest dung beetle diversity is recorded in large forest areas (2.1719). The highest species abundance (186) and richness (22) were recorded in the large forest. The lowest species abundance was recorded in tea plantations (109). The lowest species richness was recorded in pinus plantations (10). Moreover, functional group diversity is high in habitats with fewer human activities. The dominant species in large forests is Onthophagus favrei (dominancy index-2.9%). In forest patches and pinus plantations, Onthophagus amphinasus (19%) and Paracopris signatus (36.30%) appeared consecutively. In home gardens and tea plantations, the most dominant species is Onthophagus unifasiatus (26.50% and 28.40%). Habitats with anthropogenic activities have less species richness and a high dominancy index. Furthermore, dung beetle resource relocation efficiency was skewed toward forest areas. The highest dung removal percentage was recorded in forest areas (large forest-9.58% and forest patches-7.33%). The dung removal in the anthropogenic activities related to land uses was decreased as a home garden (7.17%), Pinus plantation (6.58%), and tea plantation (5.42%). Moreover, large dung beetles (body mass≥0.5 g) were only recorded in large forests and forest patches. Low diversity in tea plantations could be attributed to pesticide and fertilizer usage, as beetles are highly sensitive to these chemicals. Pinus plantations showed low rates of dung removal because resource availability in those habitats is less due to the fewer mammals. These findings indicate that replacing forests and forest areas with simplified human residential areas and agricultural systems can result in shifts towards less specialized dung beetle communities with altered proportions to other animal groups. 
 Keywords: Scarabaeinae, Dung removal, Species richness, Species abundance, Dominance index

Bacterial community structure and co-occurrence networks in the rhizosphere and root endosphere of the grafted apple

BackgroundCompared with aerial plant tissues (such as leaf, stem, and flower), root-associated microbiomes play an indisputable role in promoting plant health and productivity. We thus explored the similarities and differences between rhizosphere and root endosphere bacterial community in the grafted apple system.ResultsUsing pot experiments, three microhabitats (bulk soil, rhizosphere and root endosphere) samples were obtained from two-year-old apple trees grafted on the four different rootstocks. We then investigated the bacterial community composition, diversity, and co-occurrence network in three microhabitats using the Illumina sequencing methods. Only 63 amplicon sequence variants (ASVs) out of a total of 24,485 were shared in the rhizosphere and root endosphere of apple grafted on the four different rootstocks (M9T337, Malus hupehensis Rehd., Malus robusta Rehd., and Malus baccata Borkh.). The core microbiome contained 8 phyla and 25 families. From the bulk soil to the rhizosphere to the root endosphere, the members of the phylum and class levels demonstrated a significant enrichment and depletion pattern. Co-occurrence network analysis showed the network complexity of the rhizosphere was higher than the root endosphere. Most of the keystone nodes in both networks were classified as Proteobacteria, Actinobacteriota and Bacteroidetes and were low abundance species.ConclusionThe hierarchical filtration pattern existed not only in the assembly of root endosphere bacteria, but also in the core microbiome. Moreover, most of the core ASVs were high-abundance species, while the keystone ASVs of the network were low-abundance species.

Exploring biodiversity patterns at the landscape scale by linking landscape energy and land use/land cover heterogeneity

Agricultural Biodiversity dynamics has been evaluated by social metabolism or by landscape structure-function analysis. In this study, by using ELIA modeling, we used both methods in combination to understand how the interplay between social metabolism and landscape structure-function can affect biodiversity pattern distribution. We used energy reinvestment (E) as an indicator of social metabolism and landscape heterogeneity (Le) as an indicator of landscape structure-function. We propose a research hypothesis to analyze biodiversity patterns considering four different clusters identified based on high or low E or Le. As cluster 1, we defined E as high and Le as low and associated natural ecosystems to it. These ecosystems are expected to contain high species abundance but low richness. As cluster 2, both E and Le were defined as high and semi-natural ecosystems were associated to it, where nature friendly farm system developed. In these ecosystems, high species abundance and richness are expected. Cluster 3 with low E and Le was associated intensive farmland, which is due to the simplification of the landscape. Here, low energy reinvestment and landscape heterogeneity confirm that ecosystem services related to biodiversity have been drastically reduced. Lastly, cluster 4 with low E but high Le refers to intensive mosaics of farmland and pasture. In this cluster, the biodiversity richness index is high due to spatial landscape diversity, but the biodiversity abundance index is low due to the lack of energy reinvestment. We evaluate the proposed hypothesis for biodiversity analysis in the Qazvin province, emphasizing the interplay between energy availability and landscape heterogeneity in shaping ecological communities. This study highlights the importance of understanding biodiversity patterns at spatial scale and emphasizes the need for interdisciplinary research to address conservation and sustainability challenges. Our approach would be very useful where there is lack of biodiversity data.

Assessment of fishing gear efficiency, species diversity, and socioeconomic impacts on fishermen along the Jamuna River, Bangladesh

The Jamuna River in Bangladesh is a vital source of freshwater aquatic resources and a natural breeding habitat for freshwater fish. However, recent declines in gear efficiency and species variety have significantly impacted the economic situation of fishermen. A study was conducted on 117 fishermen in Sariakandi upazilla, Bogra district, Bangladesh, from July 2022 to June 2023. The data was collected through direct visits, group discussions, and in-person interviews. The study identified 18 fishing gears and found that the seine net was the most effective gear for 8 months, with Cypriniformes being the most dominant fish order. The highest species abundance was recorded in September, while the lowest was in February. Only two types of fishing crafts were recorded during this period. The socioeconomic conditions of the fishermen were documented, with 71% being professionals and the rest being occasional. Most were over 40 years old, experienced, and had a large family. Their homes were kacha dwellings, with only 6% missing sanitary facilities and 76% using kacha latrines. Annual earnings ranged from 12500 to 169000 Tk, with 41% relying primarily on fishing and the remainder on other activities. A total of 59% of the fishermen borrowed loans from non-governmental organizations. This study shows that diminishing species diversity and total catch significantly impact fishermen's socioeconomic conditions. The government should take proper steps to protect the natural ecosystem of the Jamuna River and enhance the living needs of fishing communities in the study area.

The effect of urbanization on species composition and trophic guilds of bats (Mammalia, Chiroptera) in the Brazilian Savanna.

Urban environments present less environmental heterogeneity in relation to the natural ones, affecting the biodiversity of bats and the ecological processes in which they participate. In this way, we will identify how urbanization influences the structure of bat communities in the municipality of Goiânia, Goiás, Brazil. We compared species composition, guilds and bat richness in a gradient that crossed urban, semi-urban and natural areas in the municipality of Goiânia, contained in the Cerrado biome. We captured a total of 775 bats of 16 species distributed in three families. Urban areas had a higher species abundance, while semi-urban areas had a higher species richness. The three types of environments have different compositions, the urban one being more homogeneous, the fauna in these areas is composed of generalist species, which benefit from this process. The diversity present in semi-urban areas is a consequence of the intersection between urban and natural fauna, which is why urban expansion needs to occur in a planned manner to minimize the impacts of this process and ensure the maintenance of biodiversity.

Spatiotemporal variations in migratory bird diversity and abundance along the coast of Gochang getbol.

Tidal flats provide critical habitat for migratory waterbird species; however, populations of migratory waterbirds have significantly declined due to tidal flat loss and degradation caused by human activities, particularly in Asia. Gochang getbol is one of tidal flats located on the southwest coast of South Korea and a center of clam production. Using bird monitoring data collected at five zones (zone1 to zone5) established across Gochang getbol and near coastal area, we examined distribution patterns of migratory bird diversity and conservation-related species along the coast of Gochang getbol. The intensity of human activity ‒ mudflat culture (mostly bivalve) and aquaculture was relatively high at zone2 and zone3, occupying > 30% of 2km circular area surrounding most sample points of these zones. Zone1 and particularly zone4 contained more natural/semi-natural habitats (less disturbed mudflats and wetlands) and zone5 had smallest mudflat than others. Shannon diversity, species richness, and abundance of migratory birds differed between zones (Anova test, P ≤ 0.02) except Shannon diversity in winter. In fall, all values were higher at zone4 than zone3 and zone5. In winter, zone1 showed greatest species richness and higher abundance than zone2, zone3, and zone5. In spring, while most differences were found between zone4 and zone5, abundance at zone4 was somewhat higher than zone2. The results from the fourth corner analysis indicated that abundance of species foraging at mudflat level was positively associated with zone1 (winter) but negatively with zone3 (fall). Sandpipers were positively associated with zone4. Abundance distribution maps of conservation-related species, created by inverse distance-weighted interpolation modeling, also showed high abundance of most conservation-related species at zone4 and 1. The findings of our study suggest the importance of natural/semi-natural habitat, and the possible link between human activity and distribution patterns of migratory birds in Gochang getbol. While we need further investigation on direct response of migratory birds to human activity, areas with low human activity with more natural/semi-natural habitat, e.g., zone4 and zone1 may be crucial for the conservation of migratory birds.

Socio-Cultural Impact of Avian Diversity in the Himalayan Region: A Comprehensive Study of Bird Species in Huddu Village, Chopta, and Deoriatal

The study employed a random visualization method to count and describe bird species in the Huddu Village, Chopta, and Deoriatal regions of the Himalayas. The data, collected through global positioning system (GPS) recordings between March and April, revealed 48 bird species across these areas, belonging to 20 different families and varying orders. The observations included 33 bird species in the Huddu region, 10 in Chopta, and 9 in Deoriatal, each displaying different elevations and geographical coordinates. Aside from birds, the fieldwork documented various flora and fauna species. The research detailed the relative abundance, distribution, and feeding habitats of bird species through recorded tables. Notable high-abundance species in Huddu Village included the streaked laughing thrush, common myna, various drongo species, grey treepie, and yellow or red-vented bulbul. Conversely, low-abundance species like alpine chough, Asian koel, scarlet minivet, and fire-breasted flowerpecker were rare, with their occurrences graphically depicted in the results. The identification of bird species utilized diverse methods, including internet resources and field guides. The study contributes valuable insights into the avian biodiversity of the Himalayan regions, emphasizing the importance of random visualization techniques and recording detailed ecological characteristics.

Temporal variations of antimicrobial resistance genes in aerosols: A one-year monitoring at the puy de Dôme summit (Central France)

The recent characterization of antibiotic resistance genes (ARGs) in clouds evidenced that the atmosphere actively partakes in the global spreading of antibiotic resistance worldwide. Indeed, the outdoor atmosphere continuously receives large quantities of particles of biological origins, emitted from both anthropogenic or natural sources at the near Earth's surface. Nonetheless, our understanding of the composition of the atmospheric resistome, especially at mid-altitude (i.e. above 1000 m a.s.l.), remains largely limited. The atmosphere is vast and highly dynamic, so that the diversity and abundance of ARGs are expected to fluctuate both spatially and temporally. In this work, the abundance and diversity of ARGs were assessed in atmospheric aerosol samples collected weekly between July 2016 and August 2017 at the mountain site of puy de Dôme (1465 m a.s.l., central France). Our results evidence the presence of 33 different subtypes of ARGs in atmospheric aerosols, out of 34 assessed, whose total concentration fluctuated seasonally from 59 to 1.1 × 105 copies m−3 of air. These were heavily dominated by genes from the quinolone resistance family, notably the qepA gene encoding efflux pump mechanisms, which represented >95 % of total ARGs concentration. Its abundance positively correlated with that of bacteria affiliated with the genera Kineococcus, Neorhizobium, Devosia or Massilia, ubiquitous in soils. This, along with the high abundance of Sphingomonas species, points toward a large contribution of natural sources to the airborne ARGs. Nonetheless, the increased contribution of macrolide resistance (notably the erm35 gene) during winter suggests a sporadic diffusion of ARGs from human activities. Our observations depict the atmosphere as an important vector of ARGs from terrestrial sources. Therefore, monitoring ARGs in airborne microorganisms appears necessary to fully understand the dynamics of antimicrobial resistances in the environment and mitigate the threats they may represent.

Anthropogenic effects of habitat modification on anuran species diversity in a swamp forest area, Kenya

AbstractAnuran species are some of the good environmental indicators of habitat quality and condition in ecosystems. This is due to their amphibious life modes and sensitivity to environmental change caused by rapid expansion of agricultural activities, advancement of forest destruction, climate change and emerging infectious diseases. Understanding their diversity and distribution patterns is crucial for the development and implementation of effective species and habitat conservation strategies. The study aimed at assessing and comparing anuran biodiversity and distribution patterns between a protected (North Nandi Forest Reserve) and a non‐protected area (Kingwal swamp) to provide additional baseline information, both being among the underexplored areas in Kenya. Data were gathered in both dry and wet seasons, between October 2022 and June 2023 by employing standard sampling techniques for anurans(Visual encounter and pitfall traps with a drift fence) to maximise detection. Three habitat types‐ farmland, intermediate land and forest were exploited. A total of 1649 individuals from 21 different species, belonging to nine different genera and nine different families were recorded. Ptychadenidae was the most abundant family from the recorded anurans, while Dicroglossidae, Arthroleptidae, Pyxicephalidae and Ranidae were the least. High biodiversity was observed in the forest habitat (Protected) and least in the farmland (non‐protected). The wet season had high species abundance, diversity and richness compared to the dry season. There was a significant difference (p < 0.05) in diversity between the habitats in wet season, but no significance difference during dry season (p > 0.05) except for forest and farmland (p < 0.05). There was a significant difference in species abundance between seasons (p < 0.05), and between habitats (p < 0.05) in all seasons except for farmland and intermediate (p > 0.05), as well as farmland and forest (p > 0.05) during the wet season. Habitat type and season had no influence (p > 0.05) on species richness. Anuran biodiversity is a function of habitat diversity and season, with the associated differences in microhabitat structure providing diverse niches and ways of exploring resources. The modest sampling indicates that Kingwal Swamp and North Nandi Forest Reserve are rich and support anuran species, and the differences in anuran biodiversity between the 3 habitats demonstrates that conservation efforts continue to be a priority.

Revealing the formation mechanisms of key flavors in fermented broad bean paste

Pixian Douban (PXDB) is a popular Chinese condiment for its distinctive flavor. Broad bean fermentation (Meju) is the most important process in the formation of flavor substances. Key flavors were analyzed qualitatively and quantitatively, and metagenomic technology was applied to study the microbial diversity during broad bean fermentation. In addition, the main metabolic pathways of key flavors were explored. Results indicated that Staphylococcus_gallinarum was the main microorganism in the microbial community, accounting for 39.13%, followed by Lactobacillus_agilis, accounting for 13.76%. Aspergillus_flavus was the fungus with the highest species abundance, accounting for 3.02%. The KEGG Pathway enrichment analysis showed that carbohydrate metabolism and amino acid metabolism were the main metabolic pathways. Glycoside hydrolase and glycosyltransferase genes were the most abundant, accounting for more than 70% of the total number of active enzyme genes. A total of 113 enzymes related to key flavors and 39 microorganisms corresponding to enzymes were annotated. And Staphylococcus_gallinarum, Lactobacillus_agilis, Weissella_confusa, Pediococcus_acidilactici, Staphylococcus_kloosii, Aspergillus_oryzae, and Aspergillus_flavus played a key role in the metabolic pathway. This study reveals the formation mechanism of key flavors in fermented broad bean, it is important for guiding the industrial production of PXDB and improving product quality.

Trapping liquids may bias the results of beetle diversity assessment

Several different techniques and methods are used to capture and study beetles (Coleoptera). One option is the use of window traps with various trapping liquids. However, these liquids used in comparative studies may have a biasing effect on the results. The effectiveness of the frequently used liquid baits, involving beer, wine, vinegar, and water as the reference liquid, was compared in this study. Twenty-four traps were assigned to two habitat categories (sunny and shady) and four kinds of bait: beer, wine, vinegar, and water. During the study from June to July 2021, a total of 29,944 invertebrates were captured; of these, 3,931 individuals belonged to Coleoptera. A total of 3,825 beetles were identified, belonging to 120 species and 36 families. The most abundant family was Nitidulidae, with 3,297 adults (86% of the total). The number of arthropods differed only in the trapping liquid, and the captures were similar between beer and wine and between vinegar and water. The trapping liquid had a more significant effect on beetle abundance and species richness. In contrast, exposure had a significant effect only on the number of beetle species and a higher ratio of beetles was found in the shade. Beer and wine were very attractive and collected similar beetle communities. However, the diversity (Shannon’s index) was low due to the high abundance of several species. Traps with vinegar and water collected a similar composition and species richness. After removing sap beetles (Nitidulidae) from all traps, a significant difference was still recorded between trapping liquids in the number of individuals and species, and their communities were much more similar. Thus, at high abundances of sap beetles, it is possible to exclude them from analyses and obtain more accurate data when assessing environmental variables. The results showed that the type of trapping liquids used can have substantial effects on abundance and species composition captured beetles in traps especially for beer and wine. The beer and wine in traps can significantly influence the subsequent biodiversity assessment. We recommend the use of trapping liquids without the baiting effect to correctly assess the effect of environmental variables on beetle richness and abundance.

Species composition of macromolluscs in Barangay Talao-talao, Lucena, Quezon province, Philippines

Degradation of upland vegetation and the continuous environmental insults on coastal ecosystems negatively impact the inhabiting molluscs. In particular, macromolluscs present in the intertidal zones of coastal communities in the Philippines are directly affected by human activities and the fast-changing climate. However, a limited number of studies have investigated the effects of habitat alteration and degradation on the composition and diversity of the Philippines’ intertidal molluscs. Thus, this study assessed the species composition of macromolluscs in Lucena, Quezon, and the underlying environmental and habitat factors that may dictate the presence and abundance of these marine animals. To address these concerns, handpicking and purposive sampling of marine molluscs were conducted in the entire intertidal region of the sampling site. This study reported 62 different species at the intertidal zones of Lucena, Quezon, with species originating from the family Olividae being the most abundant organism among the sampled gastropod and bivalve species in the area. Analysis of the observational differences in molluscan habitat may highlight ecological factors affecting the diversity and abundance of the intertidal fauna. Continuous use of the shoreline for traditional fishing and the close proximity of the port of Lucena and human habitation to the coastal zone may also serve as underlying factors to the lack of live mollusc samples in the intertidal region nearest to the port. Together, these findings suggest a high species composition and abundance of macromolluscs inhabiting the intertidal zone of Lucena, Quezon, highlighting the drastic effects of changing marine ecosystems on the ability of various species to occupy a specific area in the marine environment.