The research paper deals with Jatropha curcas oil’s transesterification process using the NiO catalyst as a heterogeneous catalyst to produce Biodiesel. The batch reactor in this process. Excess methanol is needed to shift the equilibrium towards the product and enlarge Biodiesel. The research study about the effect mole ratio of methanol: Jatropha curcas oil. The ratios are 12:1, 15:1, 18:1, and temperature of the reaction of 60, 65, 70°C. Experiment verification of the predicted yield and characterization of Biodiesel produced. The analysis biodiesel product by Chemical characterization using Gas Chromatography (G-C) analysis and ASTM 6751 standard used for the physicochemical properties. The maximum process conditions for the biodiesel yield by transesterification of Jatropha curcas oil over NiO catalyst 59.8% and the mole ratio of 15:1 methanol: Jatropha curcas oil. The 65°C reaction temperature used in this process enhances the transesterification reaction by observing 5 hours with a high yield of Biodiesel.