Abstract
BackgroundLipase is an important microbial enzyme and biocatalyst in biodiesel production. The study investigated fuel properties of biodiesel produced from palm oil (PO) using lipase immobilized on Irvingia gabonensis and calcium alginate.ResultsBiodiesel yield from PO using free and immobilized lipases was highest at 35 °C and pH 7, with product yield using calcium alginate-immobilized lipase, CAIL (94.42, 96.9%) higher than using Irvingia gabonensis-immobilized lipase, IGIL (92.54, 95.8%). Biodiesel produced using immobilized lipases had similar pour point, cloud point, and kinematic viscosity, and they possessed improved fuel properties compared to free lipase biodiesel in terms of densities at 15 °C and flash point. Pour points, flash point, and kinematic viscosity of biodiesel produced using CAIL and IGIL met American and European Standards but density at 15 °C and cloud points are below both standards. CAIL and IGIL biodiesel had similar fatty acid methyl ester (FAME) compounds and consisted more of unsaturated fatty acids (hexadecanoate, 9-octadecenoate, octadecanoate, dodecanoate, and 9,12-octadeca-dienoate) than obtained in biodiesel from free lipase. IGIL and CAIL were re-used in 8 and 12 cycles respectively, with > 90% biodiesel yield achieved in four and 11 cycles.ConclusionsThe study showed that lipase immobilized on Irvingia gabenensis and calcium alginate and used in biodiesel production retained high enzyme activity and biodiesel yield in repeated cycles.
Highlights
Lipase is an important microbial enzyme and biocatalyst in biodiesel production
We investigated comparative biodiesel production from palm oil using lipase entrapped in calcium alginate and Irvingia gabonensis matrices based on fuel properties
Biodiesel yield was optimum at 35 °C for free and immobilized lipases, while the highest yield was obtained when calcium alginate-immobilized lipase was used for esterification (94.42%) compared to free lipase (94.1%) and Irvingia gabonensis-immobilized lipase (92.54%)
Summary
Lipase is an important microbial enzyme and biocatalyst in biodiesel production. The study investigated fuel properties of biodiesel produced from palm oil (PO) using lipase immobilized on Irvingia gabonensis and calcium alginate. Non-toxic, environmentally friendly, biologically produced diesel fuel alternative to substitute petroleum-based diesel [1,2,3]. It is produced via enzymatic transesterification of several types of feedstocks such as edible plant oils and animal fats, and in recent times, non-edible oils are being explored for biodiesel production [4]. Various techniques and support matrices have been used in lipase immobilization for biodiesel production This includes different forms of silica and silica gel, Kareem et al Beni-Suef University Journal of Basic and Applied Sciences (2020) 9:59 amberlite, sepabeads, sepharose, and resin supports formulated using adsorption, cross-linking and entrapment methods, etc. This includes different forms of silica and silica gel, Kareem et al Beni-Suef University Journal of Basic and Applied Sciences (2020) 9:59 amberlite, sepabeads, sepharose, and resin supports formulated using adsorption, cross-linking and entrapment methods, etc. [13, 14]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callMore From: Beni-Suef University Journal of Basic and Applied Sciences
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
