High Total Solids Content Research Articles

High-solid digestion – A comparison of completely stirred and plug-flow reactor systems

High-solid digestion (HSD) for biogas production is a resource-efficient and sustainable method to treat organic wastes with high total solids content and obtain renewable energy and an organic fertiliser, using a lower dilution rate than in the more common wet digestion process. This study examined the effect of reactor type on the performance of an HSD process, comparing plug-flow (PFR) type reactors developed for continuous HSD processes, and completely stirred-tank reactors (CSTRs) commonly used for wet digestion. The HSD process was operated in thermophilic conditions (52 °C), with a mixture of household waste, garden waste and agricultural residues (total solids content 27–28 %). The PFRs showed slightly better performance, with higher specific methane production and nitrogen mineralisation than the CSTRs, while the reduction of volatile solids was the same in both reactor types. Results from 16S rRNA gene sequencing showed a significant difference in the microbial population, potentially related to large differences in stirring speed between the reactor types (1 rpm in PFRs and 70–150 rpm in CSTRs, respectively). The bacterial community was dominated by the genus Defluviitoga in the PFRs and order MBA03 in the CSTRs. For the archaeal community, there was a predominance of the genus Methanoculleus in the PFRs, and of the genera Methanosarcina and Methanothermobacter in the CSTRs. Despite these shifts in microbiology, the results showed that stable digestion of substrates with high total solids content can be achieved in both reactor types, indicating flexibility in the choice of technique for HSD processes.

Assessment of the Biochemical Methane Potential of Wastewater and Sludge Cake from a Poultry Slaughterhouse

Objectives : This study evaluates the potential for resource recovery through anaerobic digestion of organic waste generated in a poultry slaughterhouse, specifically focusing on poultry slaughterhouse wastewater and sludge cake obtained from in-house wastewater treatment.Methods : Basic characteristics (total solids, alkalinity, etc.), total nitrogen/ammonia nitrogen, and elemental analysis (macro and trace elements) were performed to determine the properties of the samples and calculate theoretical methane potential. Experimental methane potential was determined through BMP tests, with parameters such as lag period (λ) and maximum methane production rate (CH 4 mL/g VS/d) obtained using the modified Gompertz equation.Results and Discussion : The wastewater exhibited low organic matter concentration (1.79 g VS/kg; 3.74 g COD/L), while the sludge cake showed high total solids content (TS 170.8 g/kg) and organic matter concentration (131.5 g VS/kg; 220 g COD/L). Elemental analysis revealed that the C/N ratio was 9.17 for the sludge cake and 8.24 for the wastewater, indicating high nitrogen content. Methane production modeling using the modified Gompertz equation revealed a lag period of 7.5 days and T80 (time to produce 80% of total methane) of 21 days for wastewater, and a lag period of 2.4 days and T80 of 14 days for sludge cake.Conclusion : Wastewater and sludge cake from poultry slaughterhouses show significant potential as substrates for anaerobic digestion. However, the wastewater has low organic matter content, and the sludge cake, while high in organic matter, has low moisture and high ammonia levels. Therefore, it may be appropriate to co-digest these two substrates, or to co-digest the sludge cake with other substrates that have low nitrogen content and high water content. These findings provide fundamental data for the anaerobic digestion of slaughterhouse waste and highlight the need for further research on co-digestion and continuous processes.

An Overview: Specificities and Novelties of the Cheeses of the Eastern Mediterranean

The aim of this study is to provide an overview of the specificities (milk characteristics, production process, ripening biochemistry, composition, and sensory properties) and novelties of the world-famous traditional cheeses of the Eastern Mediterranean (EM). The EM area is remarkably heterogeneous (11 countries—Egypt, Israel, Lebanon, Syria, Türkiye, Cyprus, Greece, Albania, Montenegro, Bosnia and Herzegovina, and Croatia) in terms of cheese production, but there are some common features that can be associated with EM which are connected to the difficult geoclimaticconditions (hilly terrain and hot summers). Cheesemakers resort to some preservation methods, such as high salt content (in white-brined cheeses), high total solids content (in hard cheeses), the use of hot water in the treatment of the curd (in pasta filata cheeses), the addition of some local herbs with antimicrobial properties, and the use of animal skin sacks for cheese ripening. Due to the high proportion of whey as a by-product, whey is traditionally used in EM for the production of whey cheeses. Preserving the production of traditional EM cheeses is critical to maintaining their cultural significance and meeting the demand of consumers interested in the provenance, craftsmanship, and nutritional value of these unique products.

Pelletized Straw for Biogas Production—Substrate Characterization and Methane Formation Potential

The use of agricultural residues in biogas plants is becoming increasingly important, as they represent an efficient and sustainable substrate alternative. Pelletizing straw can have positive effects on transportation, handling, and biogas production. In this study, different grain straw pellets from mobile and stationary pelleting plants in Germany as well as the corresponding untreated straw were characterized and investigated for their suitability for anaerobic digestion (AD). Therefore, tests on the biochemical methane potential (BMP) and the chemical–physical characterization of unpelletized straw and straw pellets were carried out. The characterization of the pellets and the straw revealed a high average total solid content of 91.8% for the industrially produced straw pellets and of 90.8% for the straw. The particle size distribution within the tested pellet samples varied greatly depending on the pelleting process and the pre-treatment of the straw. In addition, a high C/N ratio of 91:1 on average was determined for the straw pellets, whereas the average higher heating value (HHV) content of the pellets was 17.58 MJ kg−1. In the BMP tests, the methane production yields ranged from 260–319 normal liter (NL) CH4 kg−1 volatile solids (VS) for the straw pellets and between 262 and 289 NL CH4 kg−1 VS for the unpelletized straw. Overall, pelleting increases the methane yield on average from 274 to 286 NL CH4 kg−1 VS, which corresponds to an increase in methane yield of 4.3%. Based on the results, the feasibility of using straw pellets for AD could be confirmed, which can facilitate the possibility of increased biogas production from agricultural residues such as straw pellets and thus make the substrate supply more sustainable.

Calibration and validation of DEM parameters of food waste with high total solid content (≥20%) using physical experiments and EDEM simulations

Since discrete element method (DEM) parameters are important for the calculation accuracy of EDEM simulations, the DEM parameters of food waste with high total solid content (≥20%) are calibrated and validated by experiments and simulations in this paper. The physical properties of food waste are obtained by physical experiments, and then the optimal values of DEM model parameters recommended for food waste can be obtained from the GEMM database. Finally, the repose angle and the average torque obtained by EDEM simulations are compared with the experimental results to validate the calibrated DEM parameters of food waste. The average relative error of the repose angle between the experimental values and the simulation values is 3.23%, and the average relative error of the average torque value is 5.29%. That means the DEM parameters of food waste calibrated in this paper are correct and can be applied for simulating the mixing performance of food waste with high total solid content (≥20%).

Nutrient profiling of Wild Aonla (Emblica officinalis Gaertn.) populations in Northeast India: Assessing the potential of this fruit tree for ecological and human health restoration

Assessing the potential of native species like Emblica officinalis (Aonla) is vital for various reasons. It supports biodiversity conservation, sustainable agriculture, ecological equilibrium, human well-being, and climate resilience. This study, conducted across 30 diverse locations in Arunachal Pradesh, Northeast India, unveiled substantial morphological and nutrient variations among Aonla genotypes. Notable findings include G24's high total soluble solids content, G1's maximum acidity, and G13's elevated total carbohydrate content. G29 stood out with increased pectin, vitamin C, and phosphorus, while G19 exhibited higher total chlorophyll, carotenoids, and potassium. Additionally, genotypes displayed differences in crude protein, total starch, crude fibre, and total phenol content. Four promising genotypes (G29, G19, G24, and G1) hold the potential for genetic crop enhancement, offering benefits in local nutrition, health, soil quality, and water retention, not only in Northeast India but also beyond. This research underscores the significance of understanding and utilizing native resources to foster both ecosystems and human communities.

Heat-moisture-treated rice starches with different amylose and moisture contents as stabilizers for nonfat yogurt

Food producers are interested in enhancing native starch properties without chemical modifications. Here, rice starches were physically modified via heat-moisture treatment (HMT) using different amylose (high, medium, low, and waxy) and moisture (15 %, 20 %, 25 %, and 30 %) contents. Hydration properties and pasting viscosities differed for different amylose and moisture contents. The HMT starch with high amylose content and 15 % moisture (High_HMT15) exhibited the highest final viscosity (6.16 Pa.s), and 31.7 % and 35.1 % increased amount of resistant starch compared to native starch in the granule and paste states, respectively. Hence, High_HMT15 was used as a stabilizer for nonfat yogurt. The nonfat yogurt prepared with 1.0 % High_HMT15 as the stabilizer exhibited 37.3 % reduced whey separation, 2.8 times increased viscosity, a high total solids content, and acceptable sensory properties compared to the nonfat yogurt without starch. These HMT starches can replace chemically modified starches derived from other grains in various food applications.

Coal mine wastes: Effective mitigation of coal waste slurry and acid mine drainage through bioflocculation using mixotrophic bacteria as bioflocculants

Acid mine drainage (AMD) and coal waste slurry, byproducts of global mining and coal processing activities, represent significant environmental pollution concerns due to their acidic nature and high total suspended solids (TSS) content. The exploration of microbial treatment methods, specifically the utilization of microorganisms and their metabolic byproducts as bioflocculants, is underway to mitigate the adverse effects of these byproducts. This investigation assessed the effectiveness of 54 mixotrophic bacterial strains in treating coal waste slurry and AMD from coal mines located in Kalimantan, Indonesia. The bacterium Citrobacter freundii (strain SKC-4), cultured in SKC1 medium (comprising 225 mL of seawater from the Java Sea and 15 g/L molasses), demonstrated superior performance, reducing the TSS value from 4998.20 ppm to 16 ppm within an hour. Fourier Transform Infrared (FTIR) analysis revealed an increase in the intensity of the CO and CN groups from protein amides and CH2 from carbohydrates, suggesting an interaction between bacteria and clay minerals in the coal waste slurry. The bacteria also played a crucial role in adjusting the pH of AMD, elevating it from 3.82 to 6.07 after one hour of conditioning with a bacterial culture to AMD ratio of 1:9. The interaction between bacteria and their metabolic products was further substantiated by scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) analysis, which detected the presence of an extracellular polymeric substance (EPS). To our knowledge, this study represents the first exploration of the potential of mixotrophic bacteria as bioflocculants in the biotreatment of coal waste slurry and AMD from coal mining operations.

Examining the Influence of Ultrasounds and the Addition of Arrowroot on the Physicochemical Properties of Ice Cream

The aim of the study was to evaluate the possibility of utilizing ultrasonic pasteurization as an alternative method to the standard pasteurization technique used for ice cream mixes. In addition, the possibility of replacing commercial stabilizers (guar gum (GG) and carboxymethylcellulose (CMC)) with arrowroot was assessed. The evaluation of the ice cream involved an analysis of its chemical composition and physical properties, including X-ray diffraction and microstructure analysis. The ice cream containing arrowroot and undergoing ultrasonic pasteurization exhibited significantly higher content of total solids (47.17%), protein (16.26 [g·(100 g)−1]), and free reducing sugars while displaying a notably lower fat content (6.60 [g·(100·g)−1]). The combination of arrowroot and ultrasonic pasteurization exerted a positive effect on reducing the apparent viscosity of the ice cream mixture (166.10 mPa·s). Consequently, it led to decreased hardness (19.97 N), increased overrun (87.02%), and extended melting time (37.48 min) in comparison to ice creams incorporating GG and CMC with traditional pasteurization. The study showed that arrowroot is a promising alternative to standard commercial stabilizers (CMC and GG) in ice cream production, while ultrasound pasteurization has the potential to replace traditional pasteurization methods.

Phosphorus Recovery from Sewage Sludge as Struvite

Environmental legislation on waste management coupled with the potential for nutrient recovery are key factors encouraging the use of advanced treatment technologies to manage biosolids waste. In this context, phosphorus recovery from sewage sludge treated by a wet oxidation (WO) process was carried out in this work. High organic matter (up to 85% in COD) and total solids content (up to 75%) removal values were achieved by the WO process at elevated temperature (up to 300 °C) and pressure (up to 200 bar) conditions. The liquid and solid fractions found in the oxidation process effluent contain high amounts of phosphorus that can be recovered. This research aims to maximize its recovery in both liquid and solid fractions. In the liquid effluent, phosphorus was recovered (up to 90 mg P/L) by chemical precipitation as struvite (MgNH4PO4∙6 H2O), a slow-release fertilizer. In this case, P recoveries greater than 95% were achieved. Additionally, the solid fraction, analyzed after filtration and drying (68 mg P/gsolid), was treated by acid leaching, obtaining up to 60% phosphorus recovery. All phosphorus extracted was in orthophosphate form.

Evaluation of bioenergy production and material flow in treating Japanese concentrated Johkasou sludge using high-solid anaerobic membrane bioreactor based on one-year operation

The treatment of waste sludge generated from on-site wastewater treatment facilities (Johkasou) applied extensively in Japan is a key concern. In this study, a high-solid anaerobic membrane bioreactor (AnMBR) was continuously operated throughout a year to investigate the bioenergy production, material flow and seasonal impacts of concentrated Johkasou sludge (CJoS). The CJoS has higher carbon-to-nitrogen ratio and lipid fraction, but less proteins than waste activated sludge (WAS). The biogas yield of CJoS by AnMBR ranged from 0.47 to 0.52 L/g-VSfed, which was higher than the anaerobic digestion of WAS and lower than mixed sewage sludge from centralized wastewater treatment facilities. The greatest biogas production rate was observed during summer (0.63 L/L/d), owing to the high total organic and solid content. However, winter CJoS had the highest biogas yield and carbon conversion rate owing to the conservation of soluble organic matter in low temperature and high digestibility. The material flow results revealed that 46.5–52.4% total COD and 50.2–58.0% carbon was converted to biogas. The energy recovery rates ranged from 8.69 to 9.96 kJ/g-VSfed and the system energy self-sufficiency rates ranged from 1.92 to 3.02. The optimal dewaterability of digested sludge was achieved when polyamidine flocculant dosage was 30 mg/g-MLSS and the corresponding sludge specific resistance to filtration was 2.26 × 1012 m/kg. The results demonstrated that AnMBR yielded high energy conversion efficiency without co-digestion and pretreatment processing.

Novel Intelligent System Based on Automated Machine Learning for Multiobjective Prediction and Early Warning Guidance of Biogas Performance in Industrial-Scale Garage Dry Fermentation

Industrial-scale garage dry fermentation systems are extremely nonlinear, and traditional machine learning algorithms have low prediction accuracy. Therefore, this study presents a novel intelligent system that employs two automated machine learning (AutoML) algorithms (AutoGluon and H2O) for biogas performance prediction and Shapley additive explanation (SHAP) for interpretable analysis, along with multiobjective particle swarm optimization (MOPSO) for early warning guidance of industrial-scale garage dry fermentation. The stacked ensemble models generated by AutoGluon have the highest prediction accuracy for digester and percolate tank biogas performances. Based on the interpretable analysis, the optimal parameter combinations for the digester and percolate tank were determined in order to maximize biogas production and CH4 content. The optimal conditions for the digester involve maintaining a temperature range of 35–38 °C, implementing a daily spray time of approximately 10 min and a pressure of 1000 Pa, and utilizing a feedstock with high total solids content. Additionally, the percolate tank should be maintained at a temperature range of 35–38 °C, with a liquid level of 1500 mm, a pH range of 8.0–8.1, and a total inorganic carbon concentration greater than 13.8 g/L. The software developed based on the intelligent system was successfully validated in production for prediction and early warning, and MOPSO-recommended guidance was provided. In conclusion, the novel intelligent system described in this study could accurately predict biogas performance in industrial-scale garage dry fermentation and guide operating condition optimization, paving the way for the next generation of intelligent industrial systems.

Characterization of ‘Wonderful’ pomegranate in the state of Chihuahua, México

The consumption of fruits rich in antioxidants has increased in recent years, a clear example of this is the pomegranate (Punica granatum L.). The cultivation of the pomegranate shows great expectations due to its profitability and its adaptability to development in arid zones. In this research, ‘Wonderful’ pomegranate fruits from Coyame Chihuahua, México were used. Quality parameters and bioactive compounds were evaluated to characterize the pomegranate produced in the state of Chihuahua, six different lots from local producers were used. The results showed significance between the different treatments, lot 5 (L5) presented the best quality characteristics such as: weight, diameter, lower percentage of shell and cartilage, as well as one of the highest percentages of arils, in addition, it had a high Total Soluble Solids (TSS) content, a high Titratable Acidity (TA) and a low sugar-acidity ratio, however, lot 3 (L3) showed the highest antioxidant capacity. In general, the qualities and attributes of the Chihuahua pomegranates, obtained higher values ​​in most of the evaluated variables in comparison with other reported results. On the other hand, as a result of this research, the implementation of the color index in the peel is proposed as a tool for the prediction of the maturity index of the pomegranate. This study contributes to the producers of this fruit tree because there is little information on the production and characterization of the pomegranate.

Water deficit and salinity modify some morphometric, physiological, and productive attributes of Aloe vera (L.)

Background: Water deficit and salinity are common conditions in arid agro-ecosystems.
 Hypothesis: Salinty increases the hydric stress effect on the yield and gel quality of Aloe vera.
 Study species and data description: In A. vera plants the variables meassured were: plant height, leaf length, width, and thickness, fresh biomass, the relative content of water, total solids, gel percentage, and harvest index.
 Study site and dates: The study was conducted from spring to summer 2020 in Bermejillo, Durango, Mexico.
 Methods: A split-block experimental design with four replications was used. The large plots were two soil moisture contents: field capacity (18.5 ± 2.5 %) and permanent wilting point (12.5 ± 2.5 %). The small plot was the salinity, with levels: 0, 20, 40, 60, and 80 mM of NaCl. 
 Results:Water deficit and salinity reduced the width and thickness of leaves and, consequently, the fresh biomass and gel percentage were reduced, but gel quality increased.
 Conclusions: Water deficit and salinity, mainly the latter, reduced plant growth and leaves, and therefore, the yield of fresh biomass and gel. Although gel yield was reduced, its quality was enhanced, in terms of high total solids content.

Quality Assessment of Sludge from Filter Backwash Water in Swimming Pool Facilities

Swimming pools are examples of water-intensive facilities, where solutions for reducing economic and environmental costs are searched with increased frequency. One of the solutions supporting savings is the recovery of water from wastewater, including backwash water obtained while rinsing the filter bed. The study objective was the qualitative and quantitative assessment of post-coagulation sludges, the main pollutant found in the washings. During the analyses, assessment of the sedimentation capabilities of the sludges was performed (gravitationally), particle size distribution was assessed (particle size distribution analyser) and assessment of phytotoxicity with the use of plant indicators in short-term tests was performed (Lemna minor, Lepidium sativum, Sinapis alba, Raphanus sativus). The samples were collected from two independent circulations, which differed in terms of capacity and type of coagulant used. The tested post-coagulation sludges were characterized by high content of total suspended solids: in samples from Circuit 1 from 251 to 128 mg/L, in Circuit 2 from 489 to 228 mg/L. However, the sedimentation processes enabled significant separation of sludges. The hydrolyzed coagulant contributed to the improvement of sedimentation capabilities of the sludges. Despite the fact that in many samples low sludge concentrations favored stimulation of plant growth, the post-coagulation sludges can constitute a hazard to plant growth, particularly in the long-term perspective.

Optimisation of phosphorus recovery process from biogas slurry using straw-derived biochar coupled with Mg/La oxide as an adsorbent

Phosphorus recovery from biogas slurry can realize the management and utilization of nutrients. Phosphorus adsorption has been hindered by multiple issues, including the incompatibility of biogas slurry and the lack of phosphorus recovery research. Therefore, the key influencing factors and adsorption mechanisms of phosphorus recovery should be analysed to effectively use biogas slurry resources. In this study, a new adsorbent of straw-derived biochar coupled with Mg/La oxides (6YBC-LDO) was used as an adsorption column filler. The effects of adsorption column height, biogas slurry concentration and flow velocity on phosphorus recovery were also studied via the response surface method (RSM). The results showed that when the mass concentration, flow rate, and column height of biogas slurry were 106.24 mg/L, 2.44 mL/min, and 1.60 cm, respectively, the recovery efficiency of phosphorus was higher, and the equilibrium adsorption capacity (qequ.) could reach 287.58 mg/g. The optimised process could further improve the regeneration rate and life cycle of 6YBC-LDO. Correlation analysis of phosphorus recovery efficiency and the characteristics of biogas slurry showed that high chemical oxygen demand (COD) and total solids (TS) content have a positive correlation with the phosphorus concentration of biogas slurry. High solid content may hinder the flow rate of biogas slurry, which directly and indirectly affects the phosphorus recovery efficiency, and the results provide a scientific basis for regulating phosphorus recovery in biogas slurry.

Production of Traditional Grape Pickle Using Lactobacillus acidophilus and Investigation of the Inhibitory Effect of the Product on Bacillus cereus and Escherichia coli

Grape pickle is a traditional food that is made with grapes (Vitis vinifera), horseradish (Armoracia rusticana) and grape syrup. In this study, the survival of Bacillus cereus and Escherichia coli in grape pickles produced with or without using probiotic Lactobacillus acidophilus La-5 as well as microbiological, chemical and sensory properties of each group were examined during 35 days of ripening at 25°C and 5 months at refrigerated storage period. Molds and lactic acid bacteria (LAB) counts remained below the limit of detection (6 log in samples to be considered a probiotic product. E. coli counts rapidly declined to undetectable level within 7 days, while B. cereus numbers was found 1.56-1.72 log cfu/g at the end of the storage period. As a result, it was established that traditional grape pickle is not suitable food matrix for probiotication. High total soluble solid content (63 °Brix) and presence of horseradish in grape pickles ensure the microbiological stability as well as the safety of product.

Comparison between different parameters for anaerobic digestion technologies

Due to the increased demand for energy and the near depletion of fossil fuel sources, in addition to the problems of global warming, the world has turned to renewable energy sources as an alternative solution. One energy source is the anaerobic digestion of organic matter, such as animal or food waste. Biogas is produced from this process, used to generate electricity and heat, or processed for use as a transportation fuel and compost production for agriculture. This process is affected by many factors like the potential of hydrogen (PH), temperature, carbon/nitrogen ratio (C/N), and other factors. Dry anaerobic digestion has higher total solid content (TS) than wet anaerobic digestion. On the other hand, it has lower maintenance and construction costs compared with wet anaerobic digestion. In this research, we will highlight the operational conditions of the process in addition to its different types. It also contains an overview of this system, its working principle, its advantages, and the system component and show the best ways to improve its efficiency.

Characterisation and pre-selection of Acca sellowiana genotypes by multivariate analysis

Feijoa (Acca sellowiana) is a native Brazilian fruit with a peculiar flavour, a considerable amount of bioactive compounds and antioxidant activity. Even though this fruit tree is currently cultivated in several countries around the world, in Brazil, the process of domestication is underway, and the selection and breeding of new genotypes that are more productive and with better fruit quality is necessary. The objective of this work was to evaluate phenotypic diversity among and within progeny and to study the correlations among the quality variables of Feijoa fruit, seeking to select individuals with superior characteristics using principal component analysis. The parents who formed the progeny (families) were selected from a participatory breeding programme. We observed that individuals 47 and 93 had a combination of desirable fruit characteristics for selection, and individuals 15, 910, 98 and 410 should be selected for future crossings, as they had a high total fruit mass and soluble solid content or the highest percentage of pulp and rounded fruit shape. Larger fruit, in general, had a lower percentage of pulp. Principal component analysis is a viable tool in the pre-selection of new genotypes and potential progenitors for Feijoa breeding programmes.

Upgrading the physiochemical and sensory quality of yogurt by incorporating polyphenol-enriched citrus pomaces with antioxidant, antimicrobial, and antitumor activities.

Industrial pomaces are cheap sources of phenolic compounds and fibers but dumping them in landfills has negative environmental and health consequences. Therefore, valorizing these wastes in the food industry as additives significantly enhances the final product. In this study, the citrus pomaces, orange pomace (OP), mandarin pomace (MP), and lemon pomace (LP) were collected by a juice company and subjected to producing polyphenols and fiber-enriched fractions, which are included in functional yogurt; the pomace powder with different levels (1, 3, and 5%) was homogenized in cooled pasteurized milk with other ingredients (sugar and starter) before processing the yogurt fermentation. The HPLC phenolic profile showed higher phenolic content in OP extract, i.e., gallic acid (1,702.65), chlorogenic acid (1,256.22), naringenin (6,450.57), catechin (1,680.65), and propyl gallate (1,120.37) ppm with massive increases over MP (1.34–37 times) and LP (1.49–5 times). The OP extract successfully scavenged 87% of DPPH with a relative increase of about 16 and 32% over LP and MP, respectively. Additionally, it inhibits 77–90% of microbial growth at 5–8 μg/mL while killing them in the 9–14 μg/mL range. Furthermore, OP extract successfully reduced 77% of human breast carcinoma. Each of pomace powder sample (OP, MP, LP) was added to yogurt at three levels; 1, 3, and 5%, while the physiochemical, sensorial, and microbial changes were monitored during 21 days of cold storage. OP yogurt had the highest pH and lowest acidity, while LP yogurt recorded the reverse. High fat and total soluble solids (TSS) content are observed in OP yogurt because of the high fiber content in OP. The pH values of all yogurt samples decreased, while acidity, fat, and TSS increased at the end of the storage period. The OP yogurts 1 and 3% scored higher in color, flavor, and structure than other samples. By measuring the microbial load of yogurt samples, the OP (1 and 3%) contributes to the growth of probiotics (Lactobacillus spp) in yogurt samples and reduces harmful microbes. Using citrus pomace as a source of polyphenols and fiber in functional foods is recommended to enhance their physiochemical and sensory quality.