Total solids (TS) content is critical for thermal hydrolysis and anaerobic digestion (AD) performance, but its role in thermal-alkaline pre-treatment (TAP) is unclear. Therefore, this study aimed to reveal the key role of TS content in TAP and AD of waste activated sludge. The results showed that the optimum TS content of TAP (at 90 °C for 1 h, pH = 10) was 8 %. Sludge disintegration and methane production increased from 19.7 ± 2.2 % to 34.3 ± 2.9 % and from 167.4 ± 4.2 to 246.0 ± 6.2 mL/g volatile solids, respectively, when TS content were increased from 2 % to 8 %. A high TS content will likely promote sludge disintegration since it will reduce heat loss and improve heating efficiency. Additionally, increasing TS content from 2 % to 10 % minimized the production of intracellular reactive oxygen species by 30.4 ± 0.7 % and increased cell viability by 11.5 ± 2.6 %. In contrast, excessive TS content (i.e., ≥10 %) deteriorated the fluidity of sludge, which prevents it from disintegration. Once TS reached 10 %, the accumulation of ammonia nitrogen and volatile fatty acids reached 812.7 ± 27.4 and 1932.0 ± 5.3 mg/L, respectively, which reduced the activity of acidulase and coenzyme F420 and shifted the archaeal community from acetylotrophic to hydrogenotrophic methanogens. This article provides new insights into the TS content in TAP and AD technology.
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