The inter-stage separation scheme has always been the focus and difficulty of low-altitude high-speed aircraft design. The separation reliability of low altitude high-speed aircraft is required to be higher. Experts and scholars have carried out design research on different forms of separation schemes. In this paper, two typical kind of inter-stage separation schemes are introduced. For aircraft A, a point cold separation scheme is adopted, which is connected by four explosive bolts, and two piston push rods provide separation impulse. For aircraft B, exhaust ports are opened on both sides of the rear of the transition section. The tail section is equipped with four “X” full-motion rudders for flight control. In addition, two horizontal stabilizers were added to improve stability. Based on the comparison and analysis of different inter-stage separation schemes for The separation reliability of low altitude high-speed aircraft is required to be higher. Experts and scholars have carried out design research on different forms of separation schemes. two different types of aircraft A and aircraft B, a new high reliability interstage separation scheme is proposed, the feasibility of the scheme is verified by simulation analysis, and the design of the stage separation structure under high dynamic pressure and high speed with compact layout is realized.