Extraction High-performance Liquid Chromatography Research Articles

Incidence, stability and risk assessment for sulfonamides and tetracyclines in aqua-cultured Nile Tilapia fish of Egypt

The current study was conducted to determine sulfonamides (SAs) and tetracyclines (TCs) residuals in farmed Nile Tilapia fish (Orechromis niloticus) using the solid phase extraction (SPE) technique and high performance liquid chromatography with diode array detection (HPLC-DAD). As well, to assess the potential health risk due to the consumption of contaminated fish following its household thermal processing. Tilapia samples were collected from four governorates in Egypt; El-Fayoum, Giza, Cairo, and Alexandria. The results showed that 56.3 % (27 out of 48 samples) of fish samples were free of antibiotics, while 10.4 % and 33.3 % of samples were contaminated by SAs and TCs, respectively. Besides, oxytetracycline (OTC) showed the highest detected concentrations ranged from 52.8 to 658.5 (μg/kg), followed by chlortetracycline (OTC) (35.89–109.76 μg/kg), and tetracycline (TC) (68.8–96.7 μg/kg). While the detected SAs were between 32.89 μg/kg (sulfamethazine: SMT) and 136.43 μg/kg (sulfadimethoxine: SDM). As well, sulfamethoxazole (SMX) showed an average concentration of 52.41 μg/kg. Notably, only 7 samples (out of 21 positive samples) had residual levels exceeded the permissible limits. The study also concluded that freezing fish at −18 °C for one week had no significant effect on the stability of SAs and TCs. As well, SAs showed more stability than TCs against the thermal processing for fish. Indeed, the stability of SAs and TCs antibiotics was arranged in a descending order, shown as follows: SMT > SDM > SMX > CTC > TC > OTC. Eventually, no potential risk to the Egyptian population was found from the consumption of the contaminated fish samples by SAs and TCs.

Simultaneous Determination of Six Alkaloids in Rat Plasma with On-line SPE–HPLC Using a Homemade Four-Hydrogen Furfuryl-Based Polymer Monolithic Sorbent

A four-hydrogen furfuryl-based polymer monolithic column for solid-phase extraction was prepared via in situ radical polymerization, using tetrahydrofurfuryl methacrylate as the monomer. The monolith was characterized by scanning electron microscopy, Fourier-transform infrared spectroscopy and nitrogen adsorption–desorption method, showing relatively uniform porous structure with micro-, meso- and macro-pores and a relatively high surface area of 54.99 m2 g−1. Online solid-phase extraction–high-performance liquid chromatography was carried out to quantitatively analyze six alkaloids, including caffeine, hydroxy-α-sanshool, hydroxy-β-sanshool, hydroxy-γ-sanshool, hydroxy-γ-isosanshool and γ-sanshool in rat plasma. The results show that the prepared polymer monolithic sorbent exhibits good clean-up ability for bio-matrix, as well as good extractive ability for the six concerning alkaloids in rat plasma. Method validation shows that the correlation coefficients of the linear regressions are higher than 0.998 and the accuracy represented by spiked recovery ranges in 94.37–100.7%, 99.52–101.1%, 98.93–100.8%, 98.36–101.6%, 99.22–101.2% and 99.86–101.4% for the six alkaloids, respectively, as well as the LOD ranges in 2.10–3.90 ng mL−1 and the LOQ in 6.35–10.14 ng mL−1. Compared to the reference works, the present work is a simple and effective method for online extraction and simultaneous determination of six alkaloids in plasma with high accuracy and low cost.

Pollution characteristics of antibiotics and antibiotic resistance of coliform bacteria in the Yitong River, China.

In this study, the concentrations of nine typical antibiotics, including sulfadiazine (SD), sulfamerazine (SMR), sulfamethazine (SM2), sulfamethoxazole (SMZ), ofloxacin (OFX), ciprofloxacin (CIP), trimethoprim (TMP), oxytetracycline (OTC), and tetracycline hydrochloride (TC), were detected in the Yitong River by solid-phase extraction high-performance liquid chromatography. The concentrations of the antibiotics were analyzed. Additionally, an improved immobilized substrate enzyme substrate method (DST-enzyme substrate method) was developed and used to evaluate the antibiotic resistance of coliform bacteria to OFX, CIP, enrofloxacin (ENR), TC, sulfisoxazole (SOX), and TMP in the Yitong River. The results showed that the concentrations of the nine antibiotics ranged from nd (not detected) to 1.361 μg/L. The detection rate and concentration of OFX were the highest, followed by CIP, and the detection rate and concentration of SM2 and OTC were the lowest. The detection rate and concentrations of antibiotics were higher in August and November than those in May. The antibiotics were mainly distributed in the livestock sewage discharge and suburban domestic sewage discharge areas. Moreover, the drug resistance of total coliform bacteria to fluoroquinolones, sulfonamides, tetracyclines, and TMP varied with season.

Correlation analysis based on the hydropathy properties of non-steroidal anti-inflammatory drugs in solid-phase extraction (SPE) and reversed-phase high performance liquid chromatography (HPLC) with photodiode array detection and their applications to biological samples

In the present work we analyzed the hydrophobicity and hydrophilicity properties of several non-steroidal anti-inflammatory drugs (NSAIDs) by investigating the structural changes of the dynamic hydrogen bond network in order to predict the extraction recovery of NSAIDs from biological fluids set by solid phase extraction (SPE). This work allows investigating the relationship between theoretical descriptors and experimental data using a parameter free method with a strong correlation (Pearson correlation 0.95, p-value 0.0003).The identification and quantification of analytes in human plasma were carried out by high performance liquid chromatography coupled with photodiode array detection (HPLC-PDA) using a Kinetex Evo C18 (150 x 4.6 mm I.D) protected by a guard column and a mixture of acetonitrile and 10 mM phosphate buffer (pH 2.5) (50:50, v/v) as mobile phase at isocratic conditions. Accuracy (BIAS%) ranged within −2.33% and + 8.05% while precision (RSD%) was less than 5.73%.The mean extraction recovery of the carprofen (IS) was 84.1% and the recovery of NSAIDs from human plasma ranged between 81.9% to 86.6%. LODs and LOQs for all the investigated NSAIDs were 0.003 and 0.01 μg/mL, respectively. The method was validated according to the ICH guide line in the range 0.010–20.0 μg/mL.

ANALYSIS OF FERULIC ACID IN ARABICA COFFEE BEAN (COFFEA ARABICA L.) USING SOLID PHASE EXTRACTION–HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

Objective: The purpose of this research is quantitatively analyzing of ferulic acid in the Arabica coffee bean extract from three samples with different regions (Garut, Pangalengan, Tasikmalaya, West Java Indonesia) using Solid Phase Extraction-High Performance Liquid Chromatography (SPE-HPLC) method which is validated.
 Methods: The analysis method used reversed phase HPLC with an Enduro C 18 G (250 mm × 4.6 mm) column and detector UV 312 nm, with a mobile phase of methanol and water containing 1% (v/v) of acetic acid (42:58) at a flow rate of 1.0 ml/min and validation method was examined in linearity, Limit of Detection (LOD), Limit of Quantification (LOQ), precision, and accuracy.
 Results: The results showed that the precision of retention was 8.853 min, correlation coefficient (R) was 0.9996, and the recovery was 96.909%. The quantitative analysis of ferulic acid content in the extract of coffee from thus samples of three different regions were 0.0385%, 0.0169% and 0.0076%, respectively.
 Conclusion: The analytical method was meet the validation criteria. Ferulic acid levels results from the extraction of the digestion process and pretreatment methods of Solid Phase Extraction (SPE) is 0.0385% from Garut area, 0.0169% from Pangalengan area and 0.0076% from Tasikmalaya area.

Analysis of antibiotic resistance of Escherichia coli isolated from the Yitong River in North-east China

The Yitong River is one of the largest secondary tributaries of the Songhua River. The area where the Yitong River flows is densely populated and contains the livestock and poultry breeding areas of northeast China. These areas introduce a high risk of antibiotic contamination. In this study, the concentrations of four types of typical antibiotics including quinolones, tetracyclines, sulfonamides, and trimethoprim were determined by solid phase extraction-high performance liquid chromatography. The antibiotic resistance of Escherichia coli caused by antibiotic pollution was investigated. The concentration of total coliforms in the Yitong River was detected by the plate counting method. The antibiotic resistance of E. coli to quinolones, tetracyclines, sulfonamides, and trimethoprim was analyzed by the Kirby-Bauer method. The results showed that the concentration of total coliforms in the summer was higher than that in the spring. There was a seasonal difference in the resistance rate of E. coli to antibiotics except trimethoprim. The antibiotic resistance to fluoroquinolones was relatively low. The resistance rate to tetracyclines was higher during the summer. Moreover, resistance to several antibiotics was observed in all sections. This study provides basic data for research on pollution characteristics and prevention of antibiotic exposure in rivers.

Analysis of chemical components in Cordyceps by online gradient extraction-HPLC

Online gradient extraction-high performance liquid chromatography( HPLC) method was developed for simultaneous determination of high and low polar components in Cordyceps. The sample powder of Cordyceps was uniformly mixed with diatomaceous earth,packed into extraction tank,and installed into the HPLC system. Online gradient extraction was conducted with mobile phase at 70 ℃. The separation was performed on Zorbax SB-AQ( 4. 6 mm×150 mm,5 μm) column with 0. 1% formic acid solution-methanol as the mobile phase for gradient elution at 1. 0 mL·min~(-1). The column temperature was 30 ℃,and detection wavelength was set at 260 nm. The results showed that the high and low polar components in Cordyceps could be simultaneously extracted and separated by the developed method. Meanwhile,six high polar compounds( uracil,uridine,thymine,inosine,guanosine and adenosine) and one low polar compound( ergosterol) were identified by comparison with the reference peaks. The established method is rapid,stable and environment friendly,which is helpful to improve the quality evaluation level for Cordyceps.

Chronic pesticide mixture exposure including paraquat and respiratory outcomes among Colombian farmers.

This study explored the potential association between chronic exposure to pesticide mixtures including paraquat and respiratory outcomes among Colombian farmers. Sociodemographic and occupational data, respiratory symptoms and spirometric data were collected. Paraquat in spot urine samples were quantified with solid-phase extraction high-performance liquid chromatography. Multiple Poisson regressions with robust variance were used to determine factors associated with respiratory outcomes. Profiles of pesticide mixtures used were identified among 217 farmworkers, but profenofos and methamidophos-based mixtures were more frequent. Chronic paraquat exposure was slightly associated with self-reported asthma (PR: 1.06; 95% CI 1.00 to 1.13). Different pesticide mixtures were associated with flu, thoracic pain, allergic rhinitis, and obstructive pattern in spirometry. Although acute exposure to paraquat is low among Colombian farmers participating in the study, associations between respiratory outcomes and chronic pesticide mixtures exposure including profenofos, methamidophos or glyphosate require further specific studies.

Validation of microwave-assisted sample extraction-high performance liquid chromatography (HPLC) method for quantitative analysis of hydrolysed amino acids in aquatic biological samples

A sensitive and widely applicable High-Performance Liquid Chromatography (HPLC) with fluorescence detector (FLD) method for determination hydrolysed amino acids in fish and aquatic products was developed and validated through study. The method involved a microwave-assisted sample extraction and protein hydrolysed with the hydrochloric acid and propionic acid. The separation was performed by Shim-pack HR-ODS column with a gradient mobile phase. The total separation time was 25 min per run. The method showed satisfactory linearity, repeatability, accuracy, and limit of detection for 21 amino acids with the 1-100 mg/L calibration range based on 10 µL injection. The fish, fish feed, canned fish and seaweeds samples were analysed successfully by using this developed method.

Magnetically Modified Porous β-Cyclodextrin Polymers for Dispersive Solid-Phase Extraction High-Performance Liquid Chromatography Analysis of Sudan Dyes

Two types of magnetically modified porous β-cyclodextrin polymers (PCDPs) were successfully synthesized. The first type, named MPCDPs(C), was fabricated by co-precipitating PCDPs with Fe3O4, resulting in a Brunauer–Emmett–Teller (BET) surface area of 106.68 m2 g−1. The second type, named MPCDPs(M), was fabricated by grafting PCDPs onto the surface of magnetic microspheres, resulting in a BET surface area of 34.63 m2 g−1. Both MPCDPs were found to enable effective extraction and separation of Sudan dyes from foodstuffs and environmental water. Coupled with high-performance liquid chromatography, the limits of detection for Sudan dyes were in the range of 0.013–0.054 ng mL−1 with MPCDPs(C), and 0.028–0.039 ng mL−1 with MPCDPs(M). Recoveries were between 85.8 and 102.8% for spiked food samples and 88.3 and 103.2% for spiked water samples, suggesting that the developed methods are promising for accurate quantification of Sudan dyes at trace levels in foodstuffs and environmental water.

Microwave irradiation followed by zinc oxide based dispersive solid-phase extraction coupled with HPLC for simultaneous extraction and determination of flavonoids in Veronicastrum latifolium (Hemsl.) Yamazaki.

In this study, a sample preparation method involving microwave irradiation followed by dispersive solid-phase extraction was used to extract and separate flavonoids from Veronicastrum latifolium (Hemsl.) Yamazaki. Ethyl lactate was selected as the extraction solvent during microwave irradiation. Zinc oxide and ethanol were used as the adsorbents and the eluent, respectively, in dispersive solid-phase extraction. Several experimental parameters affected the extraction performance, such as the extraction solvent and method, the microwave irradiation time, temperature, and solid-to-liquid ratio, the type of adsorbent, the amount of sample solution, the adsorption time and method, and the type and volume of the eluent, and were investigated by single-factor and Box-Behnken design experiments in the pretreatment process. The recommended method was applied to extract flavonoids in Veronicastrum latifolium (Hemsl.) Yamazaki. The enriched flavonoids were analyzed for the first time by high-performance liquid chromatography, and seven flavonoids-vitexin, luteolin, wogonoside, apigenin, chrysoeriol, acacetin, and pectolinarigenin-were identified. Additionally, the method exhibited good linearity (r2 ≥ 0.9992) for flavonoids under the optimum conditions. Moreover, the relative standard deviation of the intraday and interday precision was less than 3.97%. The mean recoveries for flavonoids were more than 98.10%. This work is the first to report a method to extract, separate, and analyze flavonoids from Veronicastrum latifolium (Hemsl.) Yamazaki. Graphical Abstract Microwave irradiation followed by ZnO-based dispersive solid-phase extraction for the pretreatment of Veronicastrum latifolium (Hemsl.) Yamazaki samples, and high-performance liquid chromatography (HPLC) for analysis of enriched flavonoids.

Simultaneous determination of four azo dyes in paprika powder by high performance liquid chromatography using a porous aromatic framework as the solid-phase extraction sorbent

In this study, a solid-phase extraction-high performance liquid chromatography (SPE-HPLC) method was developed for the determination of Para Red, Orange Ⅱ sodium salt, Sudan Ⅰ, and Sudan Ⅱ in paprika powder based on a self-made porous aromatic framework-6 (PAF-6) solid-phase extraction cartridge. The major factors affecting the extraction efficiency and adsorption capacity of this solid-phase extraction were evaluated. Under optimized conditions, the limits of detection (LODs) and limits of quantification (LOQs) of the four target analytes ranged from 1.5 to 7.0 μg/L and 5.0 to 22.1 μg/L, respectively. The recoveries were in the range of 76.5-89.8%, and the relative standard deviation (RSD) was not more than 5.3% (n=6). Moreover, quantum chemistry calculations were performed to investigate the molecular interaction mechanism between the PAF-6 and target analytes. The results indicated that the target molecules could be stably adsorbed onto the PAF-6. The method is accurate, reliable, and sensitive, and can be successfully applied to detect the four azo dyes in paprika powder.

Determination of organic ultraviolet filters in environmental waters by magnetic solid phase extraction-high performance liquid chromatography

A method for the determination of organic ultravioletfilters (OUFs) in water samples was developed by the combination of polymeric ionic liquid-based magnetic solid-phase extraction (PIL-MSPE) and high performance liquid chromatography-diode array detection (HPLC-DAD). To achieve the optimum extraction performance for the target analytes, several key variables, including desorption solvent, adsorption and desorption time, sample pH value and ionic strength in the sample matrix, were investigated in detail. Under the most favorable extraction conditions, linear behaviors were observed in the ranges of 0.5-200.0 μg/L for octyl salicylate and 0.2-200.0 μg/L for the other analytes. The limits of detection (LODs, S/N=3) and limits of quantification (LOQs, S/N=10) for the target OUFs were in the ranges of 0.009-0.13 μg/L and 0.031-0.43 μg/L, respectively. The developed PIL/MSPE-HPLC-DAD method was successfully applied to detect trace OUFs in real water samples. The spiked recoveries at different spiked levels were 71.4%-120%, and the RSDs were below 10% for all the compounds in all the samples. The proposed method has some unique merits including simple operation, fast extraction procedure, high sensitivity and environmental friendliness. It can become a useful method for the monitoring of trace OUFs in water samples.

Determination of neohesperidin dihydrochalcone and naringin dihydrochalcone in feeds by solid phase extraction-high performance liquid chromatography

A new method was established for the determination of neohesperidin dihydrochalcone (NHDC) and naringin dihydrochalcone (Naringin DC) in feeds by solid phase extraction-high performance liquid chromatography (SPE-HPLC). The samples were extracted by methanol and were purified on an HLB solid-phase extraction column. Chromatographic separation was achieved on an XB-C18 column (150 mm×4.6 mm, 5 μm) by linear gradient elution using methanol/water as the mobile phase. The analytes were detected by the diode array detector (DAD). The results revealed a good linear correlation (r>0.999) between the peak areas and mass concentrations of dihydrochalcone sweeteners in the range of 0.2-49.0 mg/L. The limits of quantification (LOQs) of NHDC and Naringin DC were 0.02 and 0.01 mg/kg, respectively. Intra- and inter-day reproducibilities were 0.7%-4.1% and 0.9%-6.0%, respectively. The spiked recoveries for the samples and relative standard deviations (RSDs) were 86.2%-105.0% and 1.0%-6.3% (n=3), respectively. It is both sensitive and repeatable for the quantitative determination of neohesperidin dihydrochalcone and naringin dihydrochalcone in feeds, and thus, can be used to effectively reduce interference in feeds.

Rapid determination of 16 polycyclic aromatic hydrocarbons in PM2.5 by microwave assisted extraction-high performance liquid chromatography

In this article, a method using magnetic stirring and microwave assisted extraction-high performance liquid chromatography has been developed for rapid determination of 16 polycyclic aromatic hydrocarbons (PAHs) in fine particulate matter (PM2.5) in ambient air. PAHs in PM2.5 captured on glass fiber filters were directly extracted only using 4 mL acetonitrile in a microwave extractor with magnetic stirring. Compared with dozens of minutes reported previously, the extraction time of this work only needs 6 min. After filtered through a hydrophobic membrane, the extract was directly injected for analysis. It also greatly shortened the duration of sample preparation. The operating procedures have been simplified, avoiding loss of analytes and systematic errors resulting from solvent replacement, concentration and nitrogen blowing and thereby ensuring a stable extraction efficiency, accurate and reliable analysis. The 16 PAHs were well separated on a ZORBAX eclipse PAH column using acetonitrile and water as mobile phases and detected by an ultraviolet detector and a fluorescence detector in tandem. Good linearity was demonstrated over the concentration range of 0.025 μg/mL to 5.000 μg/mL, with correlation coefficients being not <0.9998. The spiked recoveries were between 78.7% and 115.6%. The relative standard deviation ranged from 0.7% to 7.8%. The detection limits varied from 0.008 ng/m3 to 0.075 ng/m3. The method is rapid, environmentally friendly, accurate and sensitive with high extraction efficiency, convenient operation and strong resistance to interference. It is suitable for simultaneous determination of 16 PAHs in samples delivered in bulk.

Development of an SPE Method Using Ionic Liquid-Based Monolithic Column for On-Line Cleanup of Human Plasma and Simultaneous Determination of Five Steroid Drugs

An 1-vinyl-3-hexyl imidazolium bromide ionic liquid-based monolithic column was prepared via in situ free radical polymerization, which was used as the sorbent of on-line solid phase extraction. The prepared material was characterized by scanning electron microscopy, nitrogen adsorption–desorption instrument and mercury intrusion porosimetry, respectively, and the results showed that the homemade monolith occupied relatively uniform porous structure and high-specific surface area of 212.53 m2 g−1. On-line solid phase extraction–high-performance liquid chromatography was performed to quantitatively analyze five steroid drugs, including betamethasone, norgestrel, halcinonide, beclomethasone dipropionate and testosterone propionate in human plasma. The determination procedure was carried out following the optimization of the mobile phase composition. Methodological validation showed that correlation coefficients of the linear regressions were in the range of 0.9989–0.9992; the values of relative standard deviation for precision were in the range of 1.39–6.15% for intra-day and 0.32–6.34% for inter-day, respectively; the values of accuracy expressed by recovery were in the range of 99.02–101.98, 93.27–102.89, 102.79–104.01, 100.04–102.61 and 100.77–102.44% for the five drugs in order, respectively; the values of relative standard deviation for repeatability calculated according to retention times and peak areas of the five drugs were in the ranges of 0.28–0.76% (n = 5) and 0.84–2.63% (n = 5), respectively. The results showed that the polymer monolithic column was feasible for an on-line solid phase extraction column, exhibiting good selectivity and high permeability.

Diketopiperazine from marine bacterium Pseudoalteromonas ruthenica KLPp3

Sub-minimum inhibitory concentration levels of Pseudoalteromonas ruthenica KLPp3 extract showed antibiofilm activity against Vibrio alginolyticus and Serratia marcescens. The KLPp3 crude extract was fractionated by thin layer chromatography, flash chromatography, solid phase extraction and semi-preparative high performance liquid chromatography. The pure compounds were then identified using H-nuclear magnetic resonance, C-nuclear magnetic resonance, 2D-nuclear magnetic Resonance and mass spectrometry. Nine fractions were collected from purification with two active fractions. One fractions were identified belong to the family of diketopiperazine.

Improved determination of salicylaldoxime in water samples by liquid-liquid extraction followed by high performance liquid chromatographic analysis

For the determination of salicylaldoxime in environmental water samples, a stable and rapid method with low detection was proposed and established, based on the liquid-liquid extraction-high performance liquid chromatography with ultraviolet detector. Parameters including extraction solvent, ionic strength, solution pH, and extraction pattern were discussed for the optimal quantification of salicylaldoxime-spiked water. When the described method was applied to four spiked water samples, the obtained average extraction recovery rate was found between 87%–107% and relative standard deviation was below 6%. At the same time, good linear relationships were observed for spiked water samples from 0.01 to 10 μg/mL (R2=0.9993). In addition, the detection limit of salicylaldoxime was revealed between 0.003–0.008 μg/mL, which is two orders of magnitude lower than previously reported results. Thus, the presented method may be advantageous for the high-efficiency determination of salicylaldoxime in water samples.

2D Porous Aromatic Framework as a Novel Solid-Phase Extraction Adsorbent for the Determination of Trace BPA in Milk

A rapid and effective solid-phase extraction high-performance liquid chromatography fluorescence detection (SPE-HPLC–FLD) method, using a novel porous aromatic framework (PAF-6) as solid-phase extraction sorbent, was established to enrich and determine bisphenol (BPA) in milk and its packing samples. Parameters influencing the extraction efficiency, such as volume of the sample solutions, pH of the sample, and the eluent volume, were examined. The limits of detection and quantitation were 0.1 and 0.33 ng mL−1, respectively. The recovery of the method was desirable at 81–91% and the relative standard deviation was less than 2.35%. Besides, the PAF-6-based cartridges exhibited superior reusability for milk sample analysis. In addition, theoretical computations were performed to further understand the molecular interaction mechanism between the PAF-6 and the BPA. The results showed that PAF-6 had an excellent adsorption capability for BPA based on hydrogen bonding and the inclusion interactions of host–guest. The proposed method is sensitive, simple, and cost-saving, and provides a detection platform for the monitoring of BPA residues in real milk samples.

Screening and analysis of potentially active components in Shenxiong glucose injection using UHPLC coupled with photodiode array detection and MS/MS.

Shenxiong glucose injection, a pharmaceutical preparation containing a water extract of the roots of Salvia miltiorrhizae and ligustrazine hydrochloride, is widely used in clinical to treat cardiovascular diseases in China. The chemical components of the water extract have been reported and the cardioprotective effects of the injection have been evaluated. However, the chemical constituents of the injection and their correlations with its pharmacological effects have not been established. In this study, 13 chemical constituents of the injection have been identified or characterized by ultra-high performance liquid chromatography with diode array detection and electrospray ionization quadrupole time-of-flight tandem mass spectrometry. Besides, the potentially active compounds of this preparation that directly act on cardiac cells have been screened by cell extraction and ultra high performance liquid chromatography targeted multiple reaction monitoring. As a result, eight potentially active compounds, danshensu (1), ligustrazine hydrochloride (4), salvianolic acid I/H (7), lithospermic acid (8), salvianolic acid D (9), rosmarinic acid (10), salvianolic acid B (12), and salvianolic acid C (13), were obtained and structurally characterized from the 11 target compounds used for screening. The liquid chromatography with quadrupole time-of-flight mass spectrometry and liquid chromatography with multiple reaction monitoring tandem mass spectrometry combination method has demonstrated its potency for the screening, detection, and structural identification of bioactive compounds in a complex matrix.