Abstract

Sub-minimum inhibitory concentration levels of Pseudoalteromonas ruthenica KLPp3 extract showed antibiofilm activity against Vibrio alginolyticus and Serratia marcescens. The KLPp3 crude extract was fractionated by thin layer chromatography, flash chromatography, solid phase extraction and semi-preparative high performance liquid chromatography. The pure compounds were then identified using H-nuclear magnetic resonance, C-nuclear magnetic resonance, 2D-nuclear magnetic Resonance and mass spectrometry. Nine fractions were collected from purification with two active fractions. One fractions were identified belong to the family of diketopiperazine.

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