Abstract
Objective: The purpose of this research is quantitatively analyzing of ferulic acid in the Arabica coffee bean extract from three samples with different regions (Garut, Pangalengan, Tasikmalaya, West Java Indonesia) using Solid Phase Extraction-High Performance Liquid Chromatography (SPE-HPLC) method which is validated.
 Methods: The analysis method used reversed phase HPLC with an Enduro C 18 G (250 mm × 4.6 mm) column and detector UV 312 nm, with a mobile phase of methanol and water containing 1% (v/v) of acetic acid (42:58) at a flow rate of 1.0 ml/min and validation method was examined in linearity, Limit of Detection (LOD), Limit of Quantification (LOQ), precision, and accuracy.
 Results: The results showed that the precision of retention was 8.853 min, correlation coefficient (R) was 0.9996, and the recovery was 96.909%. The quantitative analysis of ferulic acid content in the extract of coffee from thus samples of three different regions were 0.0385%, 0.0169% and 0.0076%, respectively.
 Conclusion: The analytical method was meet the validation criteria. Ferulic acid levels results from the extraction of the digestion process and pretreatment methods of Solid Phase Extraction (SPE) is 0.0385% from Garut area, 0.0169% from Pangalengan area and 0.0076% from Tasikmalaya area.
Highlights
Coffee is one of the most desirable food commodities in the world
The material test is arabica coffee that obtained from the area Pangalengan, Garut and Tasikmalaya
Digestion is done by soaking 20 grams of powdered coffee beans into 250 ml of solvent aquabidest stirred continuously using a magnetic stirrer for 30 min
Summary
Coffee is one of the most desirable food commodities in the world. Coffee has been used as a trading ingredient since coffee can be processed into a beverage that has a delicious flavor. More than 400 billion cups are consumed each year. Coffee is grown in more than 80 tropical and subtropical countries, including Indonesia [1, 2]. Based on data from Direktorat Jenderal PPI Indonesia ranks fourth after Brazil, Vietnam and Colombia with a production of 639.305 thousand tons per year [3]. Many cultivated coffee plants in Indonesia are robusta and arabica. Indonesia was a leading producer of arabica coffee in the world market [1]
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