high-N Fertilizer Research Articles

An Electrochemical Approach to in-Situ Continuous Soil Nitrate Monitoring

Soil nitrogen is the most limiting nutrient affecting plant growth. However, due to poor soil management due to lack of soil health information, high-N fertilizer use increased exponentially. The UN has labeled nitrogen pollution one of the most important pollution issues facing humanity[1]. Not only does it affect life on land and underwater, it is also 300 times more potent at heating the climate than carbon dioxide while remaining active for 100 years in the atmosphere. Currently, soil nitrogen assessment techniques require destructive sampling where a soil core is collected then shipped to a lab to measure nitrogen content. Not only does this technique have high costs, it only measures nitrogen levels at that specific time point. In this work, we utilized commercially available screen printed electrodes (SPE) with a nitrate ionophore for low cost real-time continuous tracking of soil nitrate levels. The ionophore coating utilizes tetradodecylammonium nitrate (TDDA) as the selective reagent as it’s highly specific to nitrate ions. Previous work done using TDDA used open circuit potential (OCP) to measure nitrate concentration. Soil water content, pH, environmental noise, and other ions impact OCP thus making it unreliable for long term continuous measurements. This work utilizes electrochemical impedance spectroscopy (EIS) instead as it is more robust against the previously mentioned parameters. By opting to use a higher frequency of 1 kHz to extract nitrate concentration, we avoid environmental noise that are dominant at lower frequencies. The sensor output was calibrated in three different soil textures from 2 – 512 ppm presented in Fig. 1(a) for Clay soil. Then the sensor accuracy was validated to the gold standard cadmium reduction method presented in Fig. 1(b) showing non-significant differences with one sample only having a *p-value of 0.049. Lastly, the sensor stability was measured for 7 days continuously in soil with a coefficient of variation of 17% which is under the acceptable standard 20%.Figure 1 (a) Calibrated dose response for Clay soil with 50% water content. (b) vaidation of proposed sensor against gold standard in various soil types. (c) longitudinal study showing stablity and consistency of sensor performance in-situ Figure 1

Alternatives to maize monocropping in Mediterranean irrigated conditions to reduce greenhouse gas emissions

Winter legume cover crops or double-cropping in high N-fertilizer maize-based sprinkler-irrigated systems enhance agroecosystem diversity and potentially increase yields. However, the effects on direct N2O emissions and global warming potential (GWP) have not been fully established. For two years, in the Ebro Valley (Spain), four maize-based systems consisted of: long-season maize (Zea mays) with winter fallow period (F-LSM) the reference system; or after a leguminous cover crop (common vetch, Vicia sativa) (CC-LSM); and short-season maize after a cereal crop (barley, Hordeum vulgare) (B-SSM) or after a leguminous crop (pea, Pisum sativum) (P-SSM). They were assessed in terms of productivity, direct greenhouse gasses emissions (GHG: N2O, CH4, CO2), and global warming potential (GWP).Direct GHG emissions were measured using the static chamber technique, while soil parameters were monitored. Crop yields and nitrogen uptake were also quantified. GHG emissions linked to management and inputs were calculated to obtain GWP and greenhouse gas intensity (GHGI).The most productive system (B-SSM) obtained the highest direct (79 %, 35 %, and 30 % higher than the F-LSM, P-SSM, and CC-SSM, respectively) and scaled N2O emissions. The P-SSM system had similar N-uptake-scaled emissions to the monocropping (MC) systems. Irrigation, fertilizer, and farm operations accounted for the 26 %, 31 %, and 27 % of the total indirect emissions, respectively. Fertilizer production-related emissions in B-SSM and F-LSM systems were 172 % and 45 % higher than the average emissions in the systems with legumes (461 kg CO2eq. ha−1). Diversified systems lead to slightly higher GHGI values than the reference system (F-LSM). However, no differences were found between the F-LSM and P-SSM systems in GWP (4521 and 5512 kg CO2-eq. ha−1, respectively) or GHGI (144 and 158 kg CO2-eq. ha−1, respectively).The P-SSM system may be a potential alternative for increasing the diversification of maize-based irrigated agrosystems without increasing GHG emissions.

Spatially differentiated nitrogen supply is key in a global food–fertilizer price crisis

A regional geopolitical conflict and sudden massive supply disruptions have revealed vulnerabilities in our global fuel–fertilizer–food nexus. As nitrogen (N) fertilizer price spikes threaten food security, differentiated responses are required to maintain staple cereal yields across over- and underfertilized agricultural systems. Through integrated management of organic and inorganic N sources in high- to low-input cereal production systems, we estimate potential total N-fertilizer savings of 11% in India, 49% in Ethiopia and 44% in Malawi. Shifting to more cost-effective, high-N fertilizer (such as urea), combined with compost and integration of legumes, can optimize N in N-deficient systems. Better targeted and more efficient N-fertilizer use will benefit systems with surplus N. Geospatially differentiated fertilization strategies should prioritize high-N fertilizer supply to low-yield, N-deficient locations and balanced fertilization of N, P, K and micronutrients in high-yield systems. Nationally, governments can invest in extension and realign subsidies to enable and incentivize improved N management at the farm level.

Characteristics of annual NH3 emissions from a conventional vegetable field under various nitrogen management strategies

High N-fertilizer applications to conventional vegetable production systems are associated with substantial emissions of NH3, a key substance that triggers haze pollution and ecosystem eutrophication and thus, causing considerable damage to human and ecosystem health. While N fertilization effects on NH3 volatilization from cereal crops have been relatively well studied, little is known about the magnitude and yield-scaled emissions of NH3 from vegetable systems. Here we report on a 2-year field study investigating the effect of various types and rates of fertilizer application on NH3 emissions and crop yields for a pepper-lettuce-cabbage rotation system in southwest China. Our results show that both NH3 emissions and direct emission factors of applied N varied largely across seasons over the 2-year period, highlighting the importance of measurements spanning entire cropping years. Across all treatments varying from solely applying urea fertilizers to only using organic manures, annual NH3 emissions ranged from 0.64 to 92.4 kg N ha−1 yr−1 (or 0.07–6.84 g N kg−1 dry matter), equivalent to 0.05–5.99% of the applied N. At annual scale, NH3 emissions correlated positively with soil δ15N values, indicating that soil δ15N may be used as an indicator for NH3 losses. NH3 emissions from treatments fertilized partially or fully with manure were significantly lower compared with the urea fertilized treatment, while vegetable yields remained unaffected. Moreover, full substitution of urea by manure as compared to the partial substitution further reduced the yield-scaled annual NH3 emissions by 79.0–92.4%. Across all vegetable seasons, there is a significant negative relationship between yield-scaled NH3 emissions and crop N use efficiency. Overall, our results suggest that substituting urea by manure and reducing total N inputs by 30–50% allows to reduce NH3 emissions without jeopardizing yields. Such a change in management provides a feasible option to achieve environmental sustainability and food security in conventional vegetable systems.

Enhanced NH4+ Removal and Recovery from Wastewater Using Na-Zeolite-based Flow-Electrode Capacitive Deionization: Insight from Ion Transport Flux.

Flow-electrode capacitive deionization (FCDI) is a promising electromembrane technology for wastewater treatment and materials recovery. In this study, we used low-cost Na-modified zeolite (Na-zeolite) to prepare a composite flow-electrode (FE) suspension with a small amount of highly conductive carbon black (CB) to remove and recover NH4+ from synthetic and actual wastewater (200 mg-N/L). Compared with conventional activated carbon (AC), the Na-zeolite electrode exhibited a 56.2-88.5% decrease in liquid-phase NH4+ concentration in the FE suspension due to its higher NH4+ adsorption capacity (6.0 vs. 0.2 mg-N/g). The resulting enhancement of NH4+ diffusion to the electrode chamber contributed to the improved performance of FCDI under both constant current (CC) and constant voltage (CV) conditions. The addition of CB to the FE suspension increased the conductivity and facilitated Na-zeolite charging for NH4+ electrosorption, especially in CV mode. NH4+-rich zeolite can be easily separated by sedimentation from CB in the FE suspension, producing a soil conditioner with a high N-fertilizer content suitable for soil improvement and agricultural applications. Overall, our study demonstrates that the novel Na-zeolite-based FCDI can be developed as an effective wastewater treatment technology for both NH4+ removal and recovery as a valuable fertilizer resource.

Bacterial Diversity and Potential Functions in Response to Long-Term Nitrogen Fertilizer on the Semiarid Loess Plateau.

Bacterial diversity and its functions are essential to soil health. N fertilization changes bacterial communities and interferes with the soil biogeochemical N cycle. In this study, bacterial community and soil physicochemical properties were studied in 2018 after applying N fertilizers (0, 52.5, 105, 157.5, and 210 kg N ha−1) for a long (2003–2018) and a short (2003–2004) duration in a wheat field on the Loess Plateau of China. Soil bacteria were determined using 16S rRNA Illumina-MiSeq®, and the prediction function was analyzed through PICRUSt. The study showed that N fertilizer significantly changed the diversity and abundance of bacterial communities. The phyla Proteobacteria, Actinobacteria, Acidobacteria, and Chloroflexi were most abundant, accounting for 74–80% of the bacterial community abundance. The optimum rates of N fertilizer application (N105) maintain soil health by promoting soil microbial diversity and abundance. The bacterial population abundance was higher after short-term N application than after N application for a long duration and lowest with the high N-fertilizer treatment (N210). High N enrichment led to more heterotrophic N-fixing microorganisms (Alphaproteobacteria), in which metabolism and genetic information processing dominated, while cellular processes, genetic information processing, metabolism, and organismal systems were the main functional categories under low N. The phyla Gemmatimonadetes, Actinobacteria, Bacteroidetes, and Chloroflexi were the key bacteria in the co-occurrence network. The genus Saccharimonadales of the superphylum Patescibacteria has a more significant impact under low N treatment. Long-term N fertilization affected the soil pH, NO3-N, and other physicochemical properties, and soil NO3-N was the highest indicator, contributing 81% of the bacterial community function under different N fertilizer treatments.

Effects of water deficit and high N fertilization on wheat storage protein synthesis, gluten secondary structure, and breadmaking quality

The content and composition of wheat storage proteins are the major determinants of dough rheological properties and breadmaking quality and are influenced by cultivation conditions. This study aimed to investigate the effects of water deficit and high N-fertilizer application on wheat storage protein synthesis, gluten secondary structure, and breadmaking quality. Reverse-phase ultrahigh-performance liquid chromatography analysis showed that storage protein and gluten macropolymer accumulation was promoted under both independent applications and a combination of water-deficit and high N-fertilizer treatments. Fourier-transform infrared spectroscopy showed that water deficit and high N-fertilizer treatments generally improved protein secondary structure formation and lipid accumulation, and reduced flour moisture. In particular, high N-fertilizer application increased β-sheet content by 10.4% and the combination of water-deficit and high N-fertilizer treatments increased random coil content by 7.6%. These changes in gluten content and secondary structure led to improved dough rheological properties and breadmaking quality, including superior loaf internal structure, volume, and score. Our results demonstrate that moderately high N-fertilizer application under drought conditions can improve gluten accumulation, gluten secondary structure formation, and baking quality.

Biological nitrification inhibition by sorghum root exudates impacts ammonia-oxidizing bacteria but not ammonia-oxidizing archaea

Sorghum has a great capacity to release biological nitrification inhibitors (BNIs), but the inhibitory effect on nitrification and ammonia oxidizer populations under planted soil conditions is unclear. A pot experiment with three nitrogen (N) application rates (0, 50, and 200 mg N kg−1) was set up to detect the influence of sorghum growth on soil nitrification and investigate the function of blocking the activity of ammonia oxidizers. A 15N-labeled experiment was also conducted to detect the N form absorbed by sorghum. Sorghum root exudates were collected at 30 days after transplanting to hydroponic culture and added into cultured soil to determine the shifts in the populations of nitrifiers. The 15N labeling experiment showed that the uptake rate by sorghum of ammonium N fertilizer was 24% and that of nitrate N fertilizer was 9%, indicating that sorghum was an ammonium using plant. Compared with unplanted soil, sorghum planting had a significant inhibitory effect on the nitrification process even at the high-N fertilizer rates. Autotrophic nitrification was the prevailing process, and sorghum root exudation inhibited this process as much as dicyandiamide (DCD, 10 mg kg−1). Root exudates had a significant inhibitory effect on ammonia-oxidizing bacteria (AOB) but had no effect on ammonia-oxidizing archaea (AOA).

Productive and Environmental Consequences of Sixteen Years of Unbalanced Fertilization with Nitrogen and Phosphorus—Trials in Poland with Oilseed Rape, Wheat, Maize and Barley

Two factorial field experiments were carried out between 2003 and 2018 in the Experimental Stations in Eastern and Western Poland using four crop rotations with winter oilseed rape, winter wheat, maize and spring barley. The initial value of phosphorus (P) in Grabów soil was 69.8 mg P·kg−1 soil and in Baborówko soil it was 111.3 mg P·kg−1 soil (Egner-Riehm Double-Lactate DL). P fertilizer was added annually at 39 kg P·ha−1 under winter oilseed rape, 35 kg P·ha−1 under maize and 31 kg P·ha−1 under wheat and barley using superphosphate and nitrogen (N), which was added at five levels (30–250 kg N·ha−1) per year as ammonium nitrate in addition to controls with no added fertilizer. Through the several years of the experiment, P fertilizer had no effect on crop N use efficiency (NUE) nor crop productivity. There was significant soil P mining particularly in the high-N fertilizer trials causing a reduction in the content of available soil P by up to 35%. This work recommends that, based on soil P analysis, P fertilizer should not be added to high-P soils. This practice may continue uninterrupted for several years (16 in this case) until the excess soil P has been reduced. This mechanism of removal of “legacy” P from soil has major implications in reducing runoff P into the Baltic Sea drainage area and other water bodies.

Dynamic proteome changes of wheat developing grains in response to water deficit and high-nitrogen fertilizer conditions

This study investigated grain proteomic profiles in response to water deficit, high nitrogen (N) fertilizer, and their combined treatments in elite Chinese bread wheat cultivar Jingdong 17, using a two-dimensional difference gel electrophoresis (2D-DIGE)-based approach. Water deficit negatively affected the main agronomic traits of wheat and grain yield, while high-N fertilizer had the opposite effects. The application of a high-N fertilizer under water deficit conditions moderately improved kernel development and grain yield. 2D-DIGE led to the identification of 124 differentially accumulated protein (DAP) spots during five different grain developmental stages, corresponding to 97 unique proteins. The more significant changes of DAPs occurred at 10–20 days after flowering. DAPs were involved in carbohydrate metabolism, protein turnover, protein folding, cell cycle control, stress response, nitrogen metabolism, photosynthesis, and energy metabolism. In particular, water deficit caused a significant downregulation of proteins involved in starch biosynthesis, whereas high-N fertilizer led to a significant upregulation of proteins involved in nitrogen metabolism, carbohydrate metabolism, and starch biosynthesis. The combined treatment resulted in a moderate upregulation of DAPs related to carbohydrate metabolism, starch biosynthesis, and nitrogen metabolism. Our results indicated that high-N fertilization could alleviate yield loss caused by water deficit by promoting the accumulation of proteins involved in nitrogen and carbohydrate metabolism.

Application of Plant Growth Regulators on Soft White Winter Wheat under Different Nitrogen Fertilizer Scenarios in Irrigated Fields

Lodging in cereal crops can result in yield loss and harvesting difficulties for growers. Application of plant growth regulator (PGR) has been an indispensable management practice to reduce lodging problems that are often exacerbated during high wind growing conditions and/or high nitrogen (N)/water environments, but the data is limited in the Columbia Basin of Oregon. The objective of this research was to evaluate the effect of two PGR products (chlormequat chloride-CC, trinexapac-ethyl-TE) at different rates and application timings on two soft white winter wheat varieties (ORCH-102 and SY Ovation). Crop growth (stem height and thickness), yield-related (spike density as ears m−2, seeds per spike, grain weight) and quality parameters (test weight, protein) were measured for two cropping seasons from October 2017 to July 2019 following the application of the two PGR products at tillering (GS21-26), stem elongation (GS30-32), and/or flag leaf (GS37-39) stages under a high-N fertilizer scenario. In both growing seasons, no lodging problems were recorded for any treatments. The plant height was reduced after PGR application, but the impact on stem thickness was limited. PGR application slightly affected wheat yield, yield components, testing weight, and protein level in both growing seasons. Our results suggested that the effect of PGR application is relatively limited if no lodging problem occurred.

Effects of water-deficit and high-nitrogen treatments on wheat resistant starch crystalline structure and physicochemical properties

This work investigated the effects of water-deficit and high-nitrogen (N) treatments on wheat resistant starch (RS) formation, molecular structure, and physicochemical properties. The results of consecutive 2-year field experiments revealed that water deficit significantly reduced starch granule number and diameter, amylose, RS content, RS particle size distribution, and physicochemical properties, including peak and trough viscosities, oil absorption capacity, and freeze-thaw stability. Water deficit also altered the long- and short-range structures of RS. In contrast, high-N fertilizer application significantly improved the RS content, long- and short-range structures, and physicochemical properties. Pearson correlation analysis revealed that RS content was positively correlated with total starch, amylose, rapidly digesting starch, 90th percentile of RS particle size, relative crystallinity, infrared 1047/1022 cm−1 ratio, peak and breakdown viscosities, oil absorption capacity, and freeze-thaw stability, and was negatively correlated with slowly digestible starch content, 1022/995 cm−1 ratio, and final viscosity.

Effect of high-nitrogen fertilizer on gliadin and glutenin subproteomes during kernel development in wheat (Triticum aestivum L.)

Nitrogen (N), a macronutrient essential for plant growth and development, is needed for biosynthesis of protein and starch, which affect grain yield and quality. Application of high-N fertilizer increases plant growth, grain yield, and flour quality. In this study, we performed the first comparative analysis of gliadin and glutenin subproteomes during kernel development in the elite Chinese wheat cultivar Zhongmai 175 under high-N conditions by reversed-phase ultra-performance liquid chromatography and two-dimensional difference gel electrophoresis (2D-DIGE). Application of high-N fertilizer led to significant increases in gluten macropolymer content, total gliadin and glutenin content, and the accumulation of individual storage protein components. Of 126 differentially accumulated proteins (DAPs) induced by high-N conditions, 24 gliadins, 12 high-molecular-weight glutenins, and 27 low-molecular-weight glutenins were significantly upregulated. DAPs during five kernel developmental stages displayed multiple patterns of accumulation. In particular, gliadins and glutenins showed respectively five and six accumulation patterns. The accumulation of storage proteins under high-N conditions may lead to improved dough properties and bread quality.

Interactive Effects of [CO2] and Temperature on Plant Chemistry of Transgenic Bt Rice and Population Dynamics of a Non-Target Planthopper, Nilaparvata lugens (Stål) under Different Levels of Soil Nitrogen.

Gaining a better understanding of the interactive effect of projected atmospheric CO2 level increase and the Earth’s rising temperature on plant chemistry (nutritional and defensive characteristics) of transgenic crops is essential when attempting to forecast the responses of target and non-target insects to climate change. In this study, effects of carbon dioxide (CO2; elevated versus ambient), temperature (T; high versus low), and their interactions on leaf nitrogen content (N%) and C:N ratio of transgenic Bt rice and its non-Bt isoline grown under low- and high-N fertilizer were systematically analyzed together with the resulting insect population dynamics of a non-target planthopper Nilaparvata lugens (Stâl) in open-top-chamber experiments. The results indicated that under low-N treatment, elevated CO2 at low T (i.e., eCO2) (compared to ambient CO2 at low T, i.e., CK) significantly decreased N% and Bt-toxin content and significantly increased C:N ratio in leaf sheath and leaf of Bt rice, especially during the tillering stage, whereas inverse effects of high T were shown on the plant chemistry of Bt rice, especially during heading stage. The combination of elevated CO2 and high T (i.e., Combined) (in contrast to CK) significantly increased N% and decreased C:N ratio in leaf sheath of Bt rice during the heading stage under low-N fertilizer, while significantly decreased N% and increased C:N ratio in leaf of Bt rice during the tillering stage, regardless of fertilizer-N level, and significantly increased Bt-toxin content in leaf sheath and leaf during the tillering stage under both low- and high-N. Moreover, no discernable relationships between Bt-toxin content and N% or leaf C:N ratio were observed at any CO2 or N levels evaluated. Furthermore, transgenic treatment, temperature and fertilizer-N level interactions, and CO2 and fertilizer-N level interactions all significantly affected the population dynamics of N. lugens. Specifically, high-N significantly enhanced the population dynamics of N. lugens fed on non-Bt rice grown under eTemp and Bt cultivar significantly reduced the population dynamics of N. lugens under eCO2 regardless of N fertilizer levels. The study demonstrates that the planting of transgenic Bt rice would not increase the risk of increased N. lugens severity under the combined condition of elevated CO2 and increased temperature, particularly under moderate level of N fertility.

New Breeding Techniques for Greenhouse Gas (GHG) Mitigation: Plants May Express Nitrous Oxide Reductase

Nitrous oxide (N2O) is a potent greenhouse gas (GHG). Although it comprises only 0.03% of total GHGs produced, N2O makes a marked contribution to global warming. Much of the N2O in the atmosphere issues from incomplete bacterial denitrification processes acting on high levels of nitrogen (N) in the soil due to fertilizer usage. Using less fertilizer is the obvious solution for denitrification mitigation, but there is a significant drawback (especially where not enough N is available for the crop via N deposition, irrigation water, mineral soil N, or mineralization of organic matter): some crops require high-N fertilizer to produce the yields necessary to help feed the world’s increasing population. Alternatives for denitrification have considerable caveats. The long-standing promise of genetic modification for N fixation may be expanded now to enhance dissimilatory denitrification via genetic engineering. Biotechnology may solve what is thought to be a pivotal environmental challenge of the 21st century, reducing GHGs. Current approaches towards N2O mitigation are examined here, revealing an innovative solution for producing staple crops that can ‘crack’ N2O. The transfer of the bacterial nitrous oxide reductase gene (nosZ) into plants may herald the development of plants that express the nitrous oxide reductase enzyme (N2OR). This tactic would parallel the precedents of using the molecular toolkit innately offered by the soil microflora to reduce the environmental footprint of agriculture.

Crop Rotational Effects on Yield Formation in Current Sugar Beet Production - Results From a Farm Survey and Field Trials.

In Europe, the framework for sugar beet (Beta vulgaris L.) production was subject to considerable changes and for the future it is expected that sugar beet cultivation might concentrate around the sugar factories for economic reasons. Based on data from a national sugar beet farmers’ survey and multi-year crop rotation trials, the effects of cropping interval (number of years in between two subsequent sugar beet crops) and of preceding crops on sugar yield were elucidated under current Central European management conditions. The dominating sugar beet cropping interval was ≥4 years in the farm survey with pronounced differences between regions. However, the cropping intervals 2, 3, and ≥4 years did not affect the sugar yield. Therefore, significant differences in sugar yield between regions were assumed to be caused by multiple interactions between year, site, and farmers’ skills. Throughout Germany, the dominating preceding crops in sugar beet cultivation were winter wheat (Triticum aestivum L.) and winter barley (Hordeum vulgare L.). In the field trials, the sugar yield was 5% higher after pea (Pisum sativum L.) compared to maize (Zea mays L.) as preceding crop, while differences between the preceding crops pea and winter wheat, and wheat and maize were not significant. Repeated measurements of canopy development and leaf color during the growing season revealed a higher N-availability after pea as preceding crop. However, decreased growth after maize was not completely compensated for by high N-fertilizer doses. Overall, the causes for the differences in sugar yield between the preceding crops remained open. The results do not support concerns about substantial yield losses in sugar beet production due to a reduction in the cropping interval from 3 to 2 years. Nevertheless, short rotations with maize and sugar beet might increase the risk of Rhizoctonia solani crown and root rot infestation. Leguminous crops such as pea offer the potential for higher sugar beet yield with lower N-fertilizer doses.

Comparative Phosphoproteomic Analysis under High-Nitrogen Fertilizer Reveals Central Phosphoproteins Promoting Wheat Grain Starch and Protein Synthesis.

Nitrogen (N) is a macronutrient important for plant growth and development. It also strongly influences starch and protein synthesis, closely related to grain yield and quality. We performed the first comparative phosphoproteomic analysis of developing wheat grains in response to high-N fertilizer. Physiological and biochemical analyses showed that application of high-N fertilizer resulted in significant increases in leaf length and area, chlorophyll content, the activity of key enzymes in leaves such as nitrate reductase (NR), and in grains such as sucrose phosphate synthase (SPS), sucrose synthase (SuSy), and ADP glucose pyrophosphorylase (AGPase). This enhanced enzyme activity led to significant improvements in starch content, grain yield, and ultimately, bread making quality. Comparative phosphoproteomic analysis of developing grains under the application of high-N fertilizer performed 15 and 25 days post-anthesis identified 2470 phosphosites among 1372 phosphoproteins, of which 411 unique proteins displayed significant changes in phosphorylation level (>2-fold or <0.5-fold). These phosphoproteins are involved mainly in signaling transduction, starch synthesis, energy metabolism. Pro-Q diamond staining and Western blotting confirmed our phosphoproteomic results. We propose a putative pathway to elucidate the important roles of the central phosphoproteins regulating grain starch and protein synthesis. Our results provide new insights into the molecular mechanisms of protein phosphorylation modifications involved in grain development, yield and quality formation.

Metabolite profiling of the response to high-nitrogen fertilizer during grain development of bread wheat (Triticum aestivum L.)

Wheat yield and quality are dependent largely on nitrogen (N) availability. In this study, we performed the first metabolomic analysis of the response to high-N fertilizer during wheat grain development using non-targeted gas chromatography-mass spectrometry (GC–MS). Quality parameter analyses demonstrated that high-N fertilizer application led to a significant increase in grain protein content and improvement in starch and bread-making quality. Comparative metabolomic profiling of six grain developmental stages resulted in identification of 74 metabolites, including amino acids, carbohydrates, organic acids and lipids/alcohol, which are primarily involved in carbon and N metabolism. Under high-N fertilizer treatment, numerous metabolites accumulated significantly during grain development. Principal component analysis revealed two principal components as being responsible for the variances resulting from N-fertilizer treatments. Metabolite–metabolite correlation analysis demonstrated that the high-N treatment group had a greater number of positive correlations among metabolites, suggesting that high-N fertilizer treatment induced a concerted metabolic change that resulted in improved grain development. Particularly, the high-N treatment-mediated significant accumulation of metabolites involved in the TCA cycle, starch and storage protein synthesis could be responsible for the improvement of grain yield and quality. Our results provide new insight into the molecular mechanisms of wheat grain development and yield and quality.

N2O emissions of India: an assessment of temporal, regional and sector trends

This paper estimates the national level trend of India’s N2O emissions from 1985–2005 and detailed sub-regional (594 districts) level and sector emissions for the year 2005. N2O emissions are estimated using the latest methodologies (IPCC 2006), disaggregated activity data and indigenized emission factors. The estimates show that India’s N2O emissions have grown from 144 Gg in 1985 to 267 Gg in 2005 exhibiting a compounded annual growth rate of 3.1%, which has been gradually declining from 4.7% over 1985–1990 to 2.4% over 2000–2005. N-fertilizer application contributed most to N2O emissions, a 49% share in 2005 compared to 40% in 1985. Sub-regional (district-level) distribution of N2O emissions showed rising mean and spread over the years, with average emissions per districts increasing from 305 ton N2O per year in 1990 to 450 tons in 2005. The main reason being increased use of N-fertilizer. However crop selection plays an important role in N2O emissions and there are crops providing high economic returns but low N-fertilizer requirements. Agriculture sector could contribute considerably to GDP even with very low N2O emissions. Indian agriculture practices vary widely in input applications and crop yields across states. The gradual transition from traditional to modern agriculture over past two decades has enhanced the intensity of inputs like N-fertilizer. A simple correlation based on 1985–2005 trends shows that, ceteris paribus, a 10% increase in total crop production is accompanied with a 12.4% increase in N-fertilizer application and a 9.7% increase in total N2O emissions from India.

Influence of Prokelisia Planthoppers on Amino Acid Composition and Growth of Spartina alterniflora

The effects of feeding by the planthopper Prokelisia dolus on its hostSpartina alterniflorawere examined under conditions of both high and low plant-nitrogen subsidy. Phloem feeding by P. dolus reduced the concentrations of γ-aminobutyric acid, isoleucine, leucine, lysine, threonine, and valine in S. alterniflora leaves. In contrast, glutamic acid was the only amino acid that increased in plants fed upon by planthoppers, and this increase was only observed in plants in the high N-fertilizer treatment. Planthopper feeding reduced the total concentration of amino/imino acids tested, and the concentration of essential amino acids, although this difference was not quite statistically significant. Generally, concentrations of individual amino acids in Spartina were higher in the high N-fertilizer treatment. Planthopper feeding and nitrogen fertilization also significantly impacted Spartina growth and production. Culm elongation, new leaf production, and tiller elongation were reduced and leaf mortality was increased on plants fed upon by planthoppers. Furthermore, planthoppers showed enhanced survival on the high-N plants. Nitrogen fertilization also moderated the effects of sap feeding on plant growth because the reduction of culm elongation associated with planthopper feeding was more pronounced in the low N-fertilizer treatment. Thus, feeding by P. dolus adversely affected both the nutritional quality and growth of Spartinaeffects that were tempered, in part, in plants receiving high nitrogen subsidy. Our results are discussed in the context of feeding-induced changes in plant quality and quantity as possible mechanisms mediating competitive interactions in Prokelisia planthoppers.