Hepatoprotective Effect Of Ethanolic Extract Research Articles

Study of the hepatoprotective activity of ethanol extract of local propolis in rabbits

The present study has been conducted to evaluate the hepatoprotective effect of ethanol extract of local propolis at Al-Diwaniyahprovence against CCl4 induce oxidative stress and liver dysfunction in white male rabbits .Twenty four male rabbits were randomly divided in four groups with (6) animal each , the first group was received distilled water (2.5 ml. orally) daily for three weeks then injected with distilled water at( 2.5 ml. subcutaneous) two times at (36) hours interval and considered as control group , the second group was drenched with Ethanol Extract of Propolis (EEP) at dose of (75 mg/kg .BadyWight) daily for three weeks then rabbits were injected with distilled water( 0.25 ml/kg. BadyWight. Subcutaneous) two times at (36) hours interval . The third group was pretreated with Ethanol Extract of Propolis at dose of (75 mg/kg BadyWight. orally) daily for three weeks then were injected with CCl4 at dose of( 0.25 ml/kg Bady Wight .subcutaneous) two times at (36) hour interval , whereas the last group was received distilled water( 2.5 ml.orally) daily for three weeks after that they were injected with CCl4 at dose of (0.25 ml/kg. BadyWight. Subcutaneous) two times at (36) hours interval .At the end of experiment serum samples were collected from rabbits to determine the serum Glutathione (GSH) , then animals were sacrificed where the liver was excised to be examined macroscopically and microscopically.The serum GSH level was significantly (Propapilty≤0.05) decreased after CCl4 injection . A remarkable macroscopic and microscopic pathological alterations were detected after CCl4 administration characterized by necrosis and degeneration of hepatic tissue . Pretreatment with EEP before CCl4 injections significantly reverse the marked decrease of serum GSH also there is improvement in hepatic histoarchitecture. . It was concluded that EEP had the potential antioxidant activities as well as, hepatoprotective efficacy.

Isoniazid induced hepatotoxicity and its amelioration with ethanolic extract of stem bark of Berberis lycium Royale in mice

Background: To study the hepatoprotective effect of Ethanolic extract of Stem Bark of Berberis lycium Royale in isoniazid (INH) induced hepatotoxicity in mice model.Methods: The study design was lab based randomized controlled in-vivo study in mice conducted from 9th April 2014 till 9th May 2014 at animal house of National Institute of Health, Islamabad. Group A was on normal diet and water and hepatotoxicity was produced by giving isoniazid (50mg/kg BW) in mice of Group B. Group C and D were given isoniazid (INH) plus low dose and high dose of Ethanolic extract of stem bark of Berberis Lycium Royle respectively.Results: INH induced hepatotoxicity was depicted by elevated serum LFT’s, hepatocytic ballooning, severe steatosis and inflammation. Mice getting concurrent treatment of INH, low and high dose of Ethanolic extract of Berberis Lycium Royle showed decreased serum levels of biomarkers and their liver sections manifested improved histological picture but more significant reduction in toxic effects were observed in animals receiving high dose.Conclusions: High dose of Ethanolic extract of stem bark of Berberis lycium Royale showed more marked hepatoprotective activity as compare to low doses. The hepatotoxicity of INH can be reduced by concurrent use of INH with ethanolic extracts of Berberis Lycium Royle.

EVALUATION OF HEPATOPROTECTIVE EFFECT OF ETHANOLIC EXTRACT OF ROOT OF PUNICA GRANATUM IN RATS

Objective: To evaluate the hepatoprotective effect of ethanolic extract of the root (REE) of Punica granatum.Methods: This study was conducted on adult albino Wistar rats of either sex weighing 150-200 g. Animals were divided into five groups (n=5). Liver injury was produced by carbon tetrachloride (CCl4) 1 ml/kg dissolved in olive oil (1:1) given intraperitoneally on day 1 and day 4 of the study duration of 14 days. Silymarin (50 mg/kg/d) orally was used as standard drug. Test groups received an REE of P. granatum (REE) at doses of 200 and 400 mg/kg/day orally along with CCl4. On the 15th day, all animals were sacrificed, and blood was collected. Liver was sent for histopathological examination. The hepatoprotective effect of REE was evaluated by assessment of physical parameters, histopathological examination and biochemical parameters such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and total serum bilirubin.Results: The administration of REE of P. granatum at doses of 200 and 400 mg/kg/day orally, exhibited a highly significant decrease in the rise of mean serum AST, ALT, ALP, and total bilirubin as compared to CCl4 treated group (p<0.001). Histopathological examination of the liver also suggested hepatoprotective effect of REE of P. granatum by restoration of hepatic architecture toward normal. Decrease in the extent of centrilobular necrosis was observed in REE (200 and 400 mg/kg/day) treated rats when compared to CCl4 treated group.Conclusion: This study demonstrated hepatoprotective activity of REE of P. granatum against CCl4 induced liver injury in rats.

EVALUATION OF HEPATOPROTECTIVE AND ANTIOXIDANT EFFECT OF COMBRETUM ALBIDUM G. DON AGAINST CCl4 INDUCED HEPATOTOXICITY IN RATS

<p><strong>Objective: </strong>The present investigations were undertaken to evaluate the hepatoprotective and antioxidant activity of the ethanolic extract of the whole plant of <em>Combretum albidum G </em>Don against CCl<sub>4</sub>-induced hepatotoxicity in rats.</p><p><strong>Methods</strong><strong>:</strong><strong> </strong>Hepatoprotective effect of ethanolic extract of <em>Combretum albidum</em> (EECA) was determined by using carbon tetrachloride (CCl<sub>4</sub>) intoxication of rats as experimental models. The extent of liver damage and effect of the plant extract was assessed by various biochemical parameters like alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total billrubin (TB) and total protein (TP) in blood serum and concentration of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-s-transferase (GST) in liver were determined. Histopathological changes in the liver of different groups were also studied.</p><p><strong>Results: </strong>The administration of EECA at dose levels of 250 and 500 mg/kg/b.w., orally had decreased the rise of ALT, AST, ALP, TB and TBRAS levels and the effects were comparable to standard drug (Silymarin 25 mg/kg/b. w,) the GSH, SOD, CAT, GPx, GST and TP levels were significantly increased in the animals received EECA. The histopathological studies show decreased necrosis and hepatocellular degeneration when compared to the CCl intoxicated liver.</p><p><strong>Conclusion: </strong>This study demonstrates that the hepatoprotective and the antioxidant activity of the whole plant of <em>Combretum albidum</em> therefore scientifically supports the use of this plant in traditional medicine for treatment of liver disorders.</p>

Inhibitory activity of the protein carbonylation and hepatoprotective effect of the ethanol-soluble extract of Caesalpinia coriaria Jacq

Free radical oxygen species cause protein carbonylation, an irreversible oxidative damage associated with diseases such as liver injury, diabetes mellitus, arthritis, cancer and Alzheimer. To determine the inhibitory activity of the protein carbonylation and the hepatoprotective effect of ethanol extract of the fruits of Caesalpinia coriaria Jacq (Fabaceae). Scavenging activity of 2,2-diphenyl-1- picrylhydrazyl radical (DPPH•) and the radical cation 2,2’-azino-bis(3-ethylbenzothiazoline-sulfonic acid) (ABTS•+) of the extract of C. coriaria at 500 mg / L, was evaluated in a preliminary test. Inhibitory activity of the protein carbonylation isolated from mouse liver using Western methodology with dinitrophenylhydrazine (DNPH) probes was evaluated. The hepatoprotective activity of the extract was evaluated in vivo in Wistar rats using carbon tetrachloride (CCl4) as toxic orally. The degree of hepatoprotection was determined by measuring the serum liver enzymes, aspartate aminotransferase (AST) and alanine aminotransferase (ALT) and by histopathological analysis of rat liver. The rate of capture of the radicals DPPH• and ABTS•+ by the extract of C. coriaria was higher than 90 % in both cases. The extract also showed a better inhibitory effect on the carbonylation of the proteins than butyl hydroxytoluene (BHT) used as positive control. The extract of C. coriaria produced a significant reduction of AST concentration (from 1667,67 ± 394,27 to 718,00 ± 24,85 U/L ) and ALT (from 967,67 ± 118,30 to 625.67 ± 60,98 U/L), indicating liver cell damage attenuation. The extract of C. coriaria produced an important antioxidant activity and moderate hepatoprotective effect against CCl4-induced toxicity.

Hepatoprotective Effect of Ethanolic Extract of Lavandula officinalis L. in Alloxan-Induced Diabetic Rats

Background: Plants have been the basis for medical treatments throughout much of human history. Although the active compounds of many herbal drugs are unknown, they have been widely prescribed by traditional medicine practitioners due to their beneficial effects and low cost. Although the primary feature of diabetes is hyperglycemia, diabetic disease can cause damage in several organs, such as the liver, over the long term. Objectives: The present study was conducted to determine the hepatoprotective activity of Lavandula officinalis L. ethanolic extract (LOE) in alloxan-induced diabetic rats. Materials and Methods: Twenty-eight male Wistar rats (weight 200 - 250 g) were randomly divided into four groups as follows: the healthy control group (HC) received saline (0.9% intraperitoneally [i.p.]) on all experimental days, the diabetic control group (DC) received a single dose of alloxan (120 mg/kg i.p.), the first treatment group (FT) received a single dose of alloxan and then 100 mg/kg of LOE i.p. for 21 days, and the second treatment group (ST) received a single dose of alloxan and then 200 mg/kg LOE i.p. for 21 days. After completion of the experimental period, liver tissue was dissected to measure the activity of certain antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT) and lipid peroxidation (MDA) by spectrophotometry. Blood samples were collected to measure aspartate transaminase (AST) and alanine transaminase (ALT) in the serum, using commercially available diagnostic kits. Results: This study revealed that intraperitoneal administration of LOE for 21 days provided significant hepatoprotection against alloxan-induced elevation in the serum marker enzymes AST (38%) and ALT (38.65%, P < 0.05), the liver antioxidant enzyme SOD (68.61%, P < 0.01), CAT (41.53%), and liver lipid peroxidation (44.74%, P < 0.05) compared to the DC group. Conclusions: The present study indicated a hepatoprotective effect of LOE in diabetic rats.

Hepatoprotective effect of ethanolic extract of whole plant of Andrographis paniculata against CCl4-induced hepatotoxicity in rats

Liver diseases pose a major challenge to international public health. Hepatic damage is associated with distortion of metabolic functions of the liver. The present study was carried out to evaluate the hepatoprotective effect of the ethanolic extract of whole plant of Andrographis paniculata in CCl4-induced hepatotoxicity in albino rats. Hepatotoxicity was induced in rats by single intraperitoneal injection of 30 % CCl4 suspended in olive oil (1 ml /kg b.w.) after every 72 h for 10 days (group II). Hepatotoxicity-induced rats were treated with 200 mg/kg b.w. of ethanolic extract of A. paniculata for 10 days, and also, CCl4 was given as in group II rats. The effect of the drug was compared with the standard drug silymarin. The effect of the ethanolic extract of whole plant of A. paniculata on marker enzymes, serum glucose, urea, creatinine, serum bilirubin total protein, A/G ratio, gamma-glutamyl transpeptidase, lipid profile and antioxidants was determined. Treatment with A. paniculata extract showed a significant reduction in blood glucose levels, marker enzymes and also lipid profile. The drug significantly increased the activity of antioxidant enzymes in the liver of hepatotoxic rats. The results of the present study suggest that treatment with A. paniculata extract enhanced the recovery from CCl4-induced hepatic damage due to its antioxidant and hepatoprotective property.

Hepatoprotective potential of ethanolic extract of Pandanus odoratissimus root against paracetamol-induced hepatotoxicity in rats

Background:Pandanus odoratissimus (Pandanaceae) is popular in the indigenous system of medicines like Ayurveda, Siddha, Unani and Homoeopathy. In the traditional system of medicine various plant parts such as leaves, root, flowers, and oils are used as anthelmintic, tonic, stomachic, digestive and in the treatment of jaundice and various liver disorders.Objective:The aim was to investigate the hepatoprotective activity of ethanolic extract of the root of P. odoratissimus against paracetamol (PCM) induced hepatotoxicity in rats.Materials and Methods:Hepatotoxicity was induced in male Wistar rat by PCM (2 g/kg b.w. p.o. for 7 days). The ethanolic extract of P. odoratissimus root was administered at the dose level of 200 mg/kg and 400 mg/kg b.w. orally for 7 days and silymarin (100 mg/kg b.w. p.o.) as standard drug was administered once daily for a week. The hepatoprotective effect of ethanolic extract was evaluated by assessment of biochemical parameters such as serum glutamic oxaloacetic transaminase, serum glutamic-pyruvic transaminase, serum alkaline phosphatase, total and direct bilirubin and triglycerides. Histopathological study of rat liver was also done.Results:Experimental findings revealed that the extract at dose level of 200 mg/kg and 400 mg/kg of b.w. showed dose dependant hepatoprotective effect against PCM induced hepatotoxicity by significantly restoring the levels of serum enzymes to normal that was comparable to that of silymarin, but the extract at dose level of 400 mg/kg was found to be more potent when compared to that of 200 mg/kg. Besides, the results obtained from histopathological study also support the study.Conclusion:From the results, it can be concluded that ethanolic extract of the root of P. odoratissimus afforded significant protection against PCM induced hepatotoxicity in rats.

Antioxidant, Hepatoprotective Potential and Chemical Profiling of Propolis Ethanolic Extract from Kashmir Himalaya Region Using UHPLC-DAD-QToF-MS.

The aim of this study was to examine hepatoprotective effect of ethanolic extract of propolis (KPEt) from Kashmir Himalaya against isoniazid and rifampicin (INH-RIF) induced liver damage in rats. Hepatic cellular injury was initiated by administration of INH-RIF combination (100 mg/kg) intraperitoneal (i.p.) injection for 14 days. We report the protective effects of KPEt against INH-RIF induced liver oxidative stress, inflammation, and enzymatic and nonenzymatic antioxidants. Oral administration of KPEt at both doses (200 and 400 mg/kg body weight) distinctly restricted all modulating oxidative liver injury markers and resulted in the attenuation of INH-RIF arbitrated damage. The free radical scavenging activity of KPEt was evaluated by DPPH, nitric oxide, and superoxide radical scavenging assay. The components present in KPEt identified by ultra high performance liquid chromatography diode array detector time of flight-mass spectroscopy (UHPLC-DAD-QToF-MS) were found to be flavonoids and phenolic acids. The protective efficacy of KPEt is possibly because of free radical scavenging and antioxidant property resulting from the presence of flavonoids and phenolic acids.

Hepatoprotective potential of ethanolic extract of Caesalpenia crista leaves against paracetamol induced hepatotoxicity in rats

Objective: To investigate the hepatoprotective activity of ethanolic extract of leaves of Caesalpenia crista (C. crista) against paracetamol induced hepatotoxicity in rats. Methods: Paracetamol (2 g/kg body weight) was used to induce hepatotoxicity in albino rats. Ethanolic extract of leaves of C. crista was administered at the dose levels of 200 and 400 mg/kg body weight orally for 7 d. Silymarin (100 mg/kg) was used as standard drug. The hepatoprotective effect of ethanolic extract was evaluated by assessment of biochemical parameters such as serum glutamic oxaloacetic transaminase, serum glutamic pyruvic transaminase, serum alkaline phosphatase, bilirubin (total and direct), and triglycerides content. Histopathological study of rat liver was also done. Results: Administration of ethanolic extract at doses 200 mg/kg and 400 mg/kg body weight exhibited significant reduction in elevated level of serum marker enzymes, bilirubin (total and direct) and triglycerides when compared to positive control group. Conclusions: It is concluded that the ethanolic extract of C. crista leaves seems to justify the promising hepatoprotective effect on paracetamol induced liver damage in rats.

Hepatoprotective effect of ethanol extract from Berchemia lineate against CCl4-induced acute hepatotoxicity in mice

Context: The roots of Berchemia lineate (L.) DC. (Rhamnaceae) have been long used as a remedy for the treatment of some diseases in Guangxi Province, China.Objective: The present study investigates the hepatoprotective effect of Berchemia lineate ethanol extract (BELE) on CCl4-induced acute liver damage in mice.Materials and methods: Effect of BELE administrated for 7 consecutive days was evaluated in mice by the serum alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total bilirubin (TBIL), albulin (ALB), globulin (GLB), and total protein (TP) levels, as well as liver superoxide dismutase (SOD) activity and malondialdehyde (MDA) level. Moreover, histopathological examinations were also taken.Results: Compared with the model group, administration of 400 mg/kg BELE for 7 d in mice significantly decreased the serum ALT (56.25 U/L), AST (297.67 U/L), ALP (188.20 U/L), and TBIL (17.90 mol/L), along with the elevation of TP (64.67 g/L). In addition, BELE (100, 200, and 400 mg/kg, i.g.) treated mice recorded a dose-dependent increment of SOD (291.17, 310.32, and 325.67 U/mg prot) and reduction of MDA (7.27, 6.77, and 5.33 nmol/mg prot) levels. Histopathological examinations also confirmed that BELE can ameliorate CCl4-induced liver injuries, characterized by extensive hepatocellular degeneration/necrosis, inflammatory cell infiltration, congestion, and sinusoidal dilatation.Discussion and conclusion: The results indicated that BELE possessed remarkable protective effect against acute hepatotoxicity and oxidative injuries induced by CCl4, and that the hepatoprotective effects of BELE may be due to both the inhibition of lipid peroxidation and the increase of antioxidant activity.

Protective effect of Annona reticulata Linn. againest simvastatin induced toxicity in chang liver cells

The aim of the study was to evaluate the hepatoprotective effect of ethanolic extract of Annona reticulata Linn (EEAR) leaves using chang liver cell line. Simvastatin was used to induce the hepatotoxicity in the chang liver cell lines. The extract was evaluated for hepatoprotective effect in simvastatin induced hepatotoxicity in chang liver cells. The percentage viability of in the cell line was evaluated by the MTT assay [(3-(4, 5 dimethylthiazole –2 yl)-2, 5 diphenyl tetrazolium bromide) assay. The EEAR treatment significantly improved viability of chang liver cell line which was evident in morphology. The hepatoprotective activity was confirmed by estimating level of Super Oxide mutase enzyme and lipid peroxidation assay. The EEAR significantly increased super oxide mutase enzyme level and decreased lipid peroxidation. Thus the present study ascertains that the leaf extract of Annona reticulata Linn possesses significant hepatoprotective activity.

DETERMINATION OF FLAVONOIDS IN STAMEN, GYNOECIUM, AND PETALS OFMagnolia grandifloraL. AND THEIR ASSOCIATED ANTIOXIDANT AND HEPATOPROTECTION ACTIVITIES

Chromatographic analysis of flavonoids in ethyl acetate fractions of the stamen, gynoecium, and petal of Magnolia grandiflora L. by HPLC-PDA-MS/MS-ESI in the negative ionization mode was performed in this study. The results revealed the presence of eight flavonoids: apigenin 8-C-glucoside, luteolin 8-C-glucoside, quercetin 3-O-rutinoside, quercetin 3-O-galactoside, quercetin, 3-O-glucoside, kaempferol 3-O-rutinoside, isorhamnetin 3-O-glucoside, and isorhamnetin. Their quantification revealed that luteolin 8-C-glucoside is the major flavonoid and that the total phenolic content is concentrated primarily in the stamen. The antioxidant and hepatoprotective effects of ethanolic extract of the flower organs were evaluated against hepatotoxicity induced by CCl4, compared with the effects of silymarin.

Evaluation of hepatoprotective potential of ethanolic extract of Ixora pavetta against isoniazid and rifampicin induced hepatotoxicity in rats

ObjectiveThe aim of present study was to demonstrate the hepatoprotective effect of ethanolic extract of Ixora pavetta (EEIP) against isoniazid and rifampicin induced hepatotoxicity in rats. MethodRats were divided into five groups each group containing 6 animals. All rats were treated with INH + RIF in saline water at the dose of 100 mg/kg b.w., p.o. to the experimental animals for 21 days. Group I served as control administered with distilled water. In order to study the effect of EEIP in rats, 200 mg/kg b.w. and 400 mg/kg b.w. of extracts were administered to the rats in group IV and V by oral route. Silymarin (2.5 mg/kg b.w., p.o.) was used as a standard drug in this study. After the course of treatment the animals were sacrificed and blood and liver samples were collected for biochemical and histopathological studies respectively. ResultsBiochemical parametric evaluation of both the standard and EEIP (200 mg and 400 mg/kg b. wt) treated rats showed significant decrease in SGOT, SGPT, ALP, Total Bilirubin, Direct Bilirubin, and Total cholesterol. And the level of Total protein was significantly increased in both standard and EEIP treated rats when compared to toxic control group rats. The changes in biochemical parameters were supported by histological profile. ConclusionIt is concluded that the ethanolic extract of Ixora pavetta protects against rifampicin and Isoniazid-induced oxidative liver injury in rats.

Hepatoprotective Effect of Ethanolic Extract of Fresh Leaves of Cassia alata on Carbon Tetrachloride-induced Toxicity in Albino Rats

The antioxidant action of 70% ethanolic extract of fresh leaves of Cassia alata on Aspartate aminotransferase (AST) and Alanine aminotransferase (ALT) activities were examined using a model carbon tetrachloride (CCL 4 ) induced oxidative damage in the liver. The administration of the extract (2.5ml and 5ml/kg body weight) significantly (p<0.05) decreased levels of CCL4 –induced formation of AST and ALP by the liver, suggesting the role of the extract in protection against pro-oxidant liver damage. Pretreatment with the extract prior to the intra-peritoneal administration of 0.5ml/kg body weight of CCL 4 was reduced by 44% and 75% for AST at a dose of 2.5ml/kg body weight and 116% and 146% for ALP at 5ml/kg body weight. These findings are suggestive of the possible chemopreventive role of Cassia alata on liver damage.

Hepatoprotective effect of ethanolic extract of Curcuma longa on thioacetamide induced liver cirrhosis in rats

BackgroundHepatology research has focused on developing traditional therapies as pharmacological medicines to treat liver cirrhosis. Thus, this study evaluated mechanisms of the hepatoprotective activity of Curcuma longa rhizome ethanolic extract (CLRE) on thioacetamide-induced liver cirrhosis in rats.MethodsThe hepatoprotective effect of CLRE was measured in a rat model of thioacetamide-induced liver cirrhosis over 8 weeks. Hepatic cytochrome P450 2E1 and serum levels of TGF-β1 and TNF-α were evaluated. Oxidative stress was measured by malondialdehyde, urinary 8-hydroxyguanosine and nitrotyrosine levels. The protective activity of CLRE free-radical scavenging mechanisms were evaluated through antioxidant enzymes. Protein expression of pro-apoptotic Bax and anti-apoptotic Bcl-2 proteins in animal blood sera was studied and confirmed by immunohistochemistry of Bax, Bcl2 proteins and proliferating cell nuclear antigen.ResultsHistopathology, immunohistochemistry and liver biochemistry were significantly lower in the Curcuma longa-treated groups compared with controls. CLRE induced apoptosis, inhibited hepatocytes proliferation but had no effect on hepatic CYP2E1 levels.ConclusionThe progression of liver cirrhosis could be inhibited by the antioxidant and anti-inflammatory activities of CLRE and the normal status of the liver could be preserved.

Aktivitas Hepatoprotektif Ekstrak Etanol Daun Kucing-kucingan Acalypha indica L.) pada Tikus Putih (Rattus Novergicus) yang Diinduksi Parasetamol

The research has been conducted to determine hepatoprotective effect of ethanolic extract of Acalypha indica L. leaves on rats (Rattus novergicus) induced with paracetamol. The study was performed following a completely randomized design, using 45 male rats (Rattus novergicus) divided into 5 groups. Rats in group 1 (K1) were given CMC 1 % for 7 days and followed by aquadest administration 8 hours post given CMC on day 7. Group 2 was given CMC 1 % for 7 days and treated with paracetamol at dose 2,5 g/kg BW 8 hours post given CMC on day 7. Groups 3 (K3), 4 (K4), and 5 (K5) were pretreated with ethanolic extract of Acalypha indica L. leaves at the dose of 50, 100, and 200 mg/kg BW for 7 days respectively, followed by paracetamol administration at the dose of 2.5 g/kg BW in the next 8 hours on day 7. Paracetamol hepatotoxicity and extracts activity were measured based on SGPT and SGOT activity level on day 0, 8 hours after last administration of extract on day 7, and 24 hours post paracetamol administration. Data were analyzed using one way ANOVA. The result showed that the administration of ethanolic extract of Acalypha indica leaves at the dose of 50, 100, and 200 mg/kg BW was significantly reduced SGPT and SGOT level (P&lt;0,05) in rats that were induced with paracetamol. Based on the results of SGPT and SGOT serum activity, it can be concluded that ethanolic extract of Acalypha indica L. potential as hepatoprotective agent.

Hepatoprotective activity of Cleome viscosa Linn. extract against thioacetamide-induced hepatotoxicity in rats

Cleome viscosa Linn. (Family Capparidaceae) is naturalised throughout the hot and moist parts of India. Fresh leaves of this plant are used very effectively for the treatment of jaundice in the folk medicines of the Bundelkhand region of India. The hepatoprotective activity of the ethanolic extract of leaves was investigated against thioacetamide-induced hepatotoxicity in rats. The test material was found to be effectively hepatoprotective, as evidenced by biochemical parameters and histopathological studies. The hepatoprotective effect of ethanolic extract was comparable to that of silymarin, a standard hepatoprotective agent. The results of the present study support the traditional beliefs of the hepatoprotective effects of C. viscosa Linn.

세포 및 동물모델에서의 알코올에 의해 유발된 간손상에 대한 지구자 추출물의 보호효과

The hepatoprotective effect of ethanol extract from Hovenia dulcis fruit (HD) against ethanol-induced oxidative damage was investigated. Ethanol-induced reactive oxygen species (ROS) generation and liver damage on HepG2/2E1 cells were protected by 100 ㎍/mL ethanolic extract from HD. Male C57BL/6 mice were divided into 3 groups; control (NC), ethanol (ET), ethanol plus 1 g/㎏ body weight ethanolic extract of HD (ET-HD). The activities of serum alanine amintransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) were significantly increased in ethanol-treated group. However, ET-HD group showed protective effect by lowering serum activities. The ET group markedly decreased the activities of catalase (CAT), superoxide dismutase (SOD), and glutathione-s-transferase (GST) with the reduced level of glutathione (GSH) in liver. On the other hand, ET-HD group increased the activities of SOD and GST, and the level of GSH. Lipid peroxidation level, which was increased after ethanol administration, was significantly reduced in ET-HD group. Based upon these results, it could be assumed that ethanolic extract of HD protected the liver against ethanol-induced oxidative damage by possibly inhibiting the suppression of antioxidant activity and reducing the rate of lipid peroxidation in vitro and in vivo. Therefore, extract of Hovenia dulcis fruit might be used as a protective agent for ethanol-induced hepatic damages.

Evaluation of hepatoprotective activity of Cleome viscosa Linn. extract

Objectives:To evaluate the hepatoprotective activity of ethanolic extract of Cleome viscosa Linn. (Capparidaceae) against carbon tetrachloride (CCI4) induced hepatotoxicity in experimental animal models.Materials and Methods:Leaf powder of Cleome viscosa was extracted with ethanol. The hepatoprotective activity of the extract was assessed in CCI4 induced hepatotoxicity in rats. Various biochemical parameters were estimated and histopathological studies were also performed on rat liver. The hepatoprotective activity was also supported by determining a functional parameter, i.e. thiopental-induced sleep of mice poisoned with CCl4.Results:The test material was found effective as hepatoprotective, through in vivo and histopathological studies. The extract was found to be effective in shortening the thiopental induced sleep in mice poisoned with CCl4. The hepatoprotective effect of ethanolic extract was comparable to that of silymarin, a standard hepatoprotective agent.Conclusion:The results of the present study show that ethanolic extract of Cleome viscosa has significant hepatoprotective activity.