Henna Extract Research Articles

Efficacy of topical Lawsonia inermis L. (Henna) hydrogel in fluorouracil-induced hand-foot syndrome: a pilot randomized double-blind placebo-controlled clinical trial

Purpose Hand–foot syndrome (HFS) is a frequent dose-limiting adverse reaction of fluoropyrimidine drugs like capecitabine and 5-flourouracil (5-FU) in breast and gastrointestinal cancers. It has been shown that conventional application of Lawsonia inermis L. (Henna) is effective in ameliorating of the skin lesions. To increase the patient compliance, in this study we formulated a standardized topical hydrogel (H.gel) containing the hydroalcoholic extract (10%) of Henna and evaluated its clinical efficacy for the management of fluorouracil associated HFS. Material and methods The topical dosage form was standardized based on its Lawsone content. Eighteen patients suffering from HFS were randomized to receive H.gel and the placebo four times a day for 2 weeks. At the baseline and at the end of the trial, HFS grades were determined. Results and conclusions Allergic reactions following administration of H.gel were observed in one patient, while no serious adverse events occurred in the others. No statistically significant differences between two arms were observed at the baseline (p-value = 0.133), after treatment (p-value = 0.590) and grade differences (p-value = 0.193). The applied hydrogel showed less efficacy compared to the traditional method of using Henna, meaning that Lawsone may not be a good indicator for standardizing the topical dosage form.

Magnesium-doped green solar cells using natural chromophores

The current study tested the hypothesis of whether specific metal doping may show synergy with plant chromophore-based solar cells using a titanium dioxide (TiO2) electrode. A natural dye-sensitized, magnesium-doped TiO2 solar cell was assembled using the methanol extract from various western African plants including Lawsonia inermis (henna). Mg2+–TiO2 nanoparticles were applied on fluorine-doped tin oxide (FTO) glass to serve as the photoanode of the solar cells with $$I^{ - } /I_{3}^{ - }$$ electrolyte. A surface-modified TiO2 photoanode was prepared through the immersion method using a selective dopant including magnesium and potash. An inductively coupled plasma-optical emission spectrophotometer (ICP-OES) was utilized to characterize the potash dopant for comparative analysis. Instrumental analysis including ultraviolet–visible spectroscopy (UV–Vis), infrared spectroscopy (IR), and gas chromatography and mass spectrometry (GC–MS) analysis were carried out to characterize the natural henna dye extracts. The photovoltaic performance including open-circuit voltage (Voc), short circuit current density (Jsc), current (I), and power output (P) was analyzed quantitatively. ICP-OES analysis demonstrated that potash contains a composite of 26 elemental metals with K and Na accounting for 72.2% (5192.2 mg/kg) and 9.5% (682.6 mg/kg), respectively. GC–MS analysis confirmed the presence of lawsone and tocopherol in henna extracts. Among the tested samples, the Mg-doped TiO2 group generated the highest improvement in Jsc, from 0.66 to 1.28 (mA/cm2), representing a 93% increase. Our experiments demonstrated that the presence of magnesium as a doping agent improves the photogenerated electron transport and the light-harvesting performance of the henna dye to increase the overall efficiency of light-to-electricity conversion of the photovoltaic cells.

Physiological effects of henna aqueous extract on growth, yield, active constituent and anatomical structure of roselle (Hibiscus sabdariffa L.) plant

This research was conducted at the Experimental Farm of Medicinal and Aromatic Plants Research Department in El-Kanater El-Khairia, during the two successive seasons 2019 and 2020. The aim of this study is to investigate the effect of spraying roselle plants with henna aqueous extract at concentrations (10, 20, 30 and 40 g/l) on growth, yield, calyces quality (anthocyanin content and acidity %) and anatomical structure. In general, the results indicated that all doses of the henna aqueous extract significantly increased the growth, yield and calyces quality as compared with control in the two seasons. This increment was parallel to the gradual increase in the dose of henna aqueous extract from 0 up to 30 g/l. In stem anatomical studies, henna extract at 30 g/l also recorded the highest results for all characters. The anatomical structure of the leaf followed approximately the same direction in the stem.

Antimicrobial activity of crude henna extract against Gram-positive bacteria

Antimicrobial activity of crude henna extract against Gram-positive bacteria

HENNA (Lawsonia inermis) A MEDICINAL HERB EFFECTIVE IN SICKLE CELL DISEASE

Sickle cell anemia is a common disease in Oman country. In this disease, sickle-shaped cells are formed. These cells interrupt blood vessels and cause a reduction in oxygen transportation. It was founded that henna (Lawsonia inermis) can prohibit the formation of sickle cells. The Lawsone (2-Hydroxy-1,4-Naphthoquinone) is the constituents of henna which is responsible for the anti-sickling activity, by increasing the oxygen affinity of red blood cells. Hena has the anti-sickling activity which is proved by incubating aqueous and methanolic henna extracts with sickle cell disease patient's whole blood. Then for reduction to oxygen tension 2%, sodium bisulphite was added. Therefore, the percentage of sickled cells to normal red blood cells was observed at 30 minutes intervals. Henna proved a delay in the sickling process in 84% of the tested samples. Both extracts(aqueous and methanolic henna) can delay sickling for about an hour.

Formulation and evaluation of the novel herbal antibacterial gel to the treatment of cutaneous burn infections

Due to the rapid development of antibiotic resistance, the strong need for alternative strategies to tackle this problem is inevitable. The objective of this study was to prepare and evaluate the antibacterial effects of a pharmaceutical gel containing herbal extracts including Lawsonia inermis (henna) and Matricaria chamomilla. Using hydroxypropyl methylcellulose (HPMC), carboxymethylcellulose (CMC), and propylene glycol (PEG), the pharmaceutical gel was formulated and the physical properties of the formulation were specified at 37 ± 2°C. The total phenolic content (TPC) of extracts was determined using the Folin–Ciocalteu method and expressed as mg gallaic acid (GA) per gram extract (Ex). The release of the polyphenol compounds from the optimum formulation was investigated using the Franz cell device. Eventually, the disc diffusion method was used to evaluate the antibacterial activity of optimum formulation against the two pathogenic bacteria strains Staphylococcus aureus and Pseudomonas aeruginosa. The results showed that the optimum formulation was stable at least for 3 months. The TPC of the aqueous extract of henna leaves, the hydroalcoholic extract of chamomile flowers, and the optimum formulation was 57.8, 181.08, and 202.75 mg GA/g Ex, respectively. Nearly 80% of the phenolic compounds in the optimum formulation were released over 4 h. The phenolic compounds have inhibitory effects on the growth of S. aureus and P. aeruginosa. On the basis of this finding, the formulation had excellent stability, viscosity, homogeneity, extrudability, and antibacterial activity which can be employed as a topical pharmaceutical gel in cutaneous burn infection treatment.

Environmental friendly application of ultrasonic rays for extraction of natural colorant from Harmal (P. harmala) for dyeing of bio-mordanted silk

Global heat, carcinogenic, and viral diseases created due to industrial pollutants has led people to shift toward natural products particularly natural dyes in all walks of life. In this study, U.S. rays up-to 60 min, were used to extract the color in aqueous medium and employed onto silk fabric at selected conditions. It has been observed that good color strength has been found when 30 mL of acidic extract ( pH = 4.0) obtained from 6.0 g of Harmal powder containing 3.0 g/100 mL of salt as an exhausting agent was employed onto the US treated silk at 65ºC for 55 min. The utilization of the extract obtained from 7% of pomegranate as post-bio-mordant, 3% of acacia extract as meta-bio-mordant, and 7% of henna extract as meta-bio-mordants has given excellent color strength. In comparison, 5% of Al salt as meta mordant, 5% of Fe salt as pre mordant, and 7% of tannic acid as meta-mordant has given better results. ISO standard methods for fastness employed onto optimum mordanted dyed fabrics have given well to excellent fastness ratings. Hence, natural colorant extracted from Harmal seeds has excellent coloring efficacy to dye silk fabric under sustainable conditions, whereas the addition of eco-friendly mordants for shade development process has made the coloration of silk more sustainable with improved colorfastness properties.

Extracts of leaves as corrosion inhibitors – An overview and corrosion inhibition by an aqueous extract of henna leaves (Lawsonia inermis)

Extracts of leaves as corrosion inhibitors – An overview and corrosion inhibition by an aqueous extract of henna leaves (Lawsonia inermis)

The effect of henna and linseed herbal ointment blend on wound healing in rats with second-degree burns

The treatment of skin burns is one of the most important challenge in medical science. The aim of this study is evaluation of the efficacy of Artaderm herbal ointment containing the Henna (Lawsonia inermis) extract, Linseed (Linum usitatissimum) oil, and Honey Wax on wound healing in the rat with second-degree burn wounds. The Artaderm ointment had an effective role in controlling burn wound infections due to its antimicrobial and anti-inflammatory properties. In this study, 64 male Wistar rats were randomly divided into 8 groups (n = 8). Four groups received Artaderm, 1% Silver Sulfadiazine (SSD 1%), Cod Liver Oil and Fundermol (Alpha) ointments which used in common practices for burn injuries. Another three groups received Henna, Linseed, and Honey Wax alone and a control group that just underwent a second-degree burn injury without any treatments. A second-degree burn was formed on the back of each rat and dressed daily with one of the agents. Burn wounds were macroscopically and microscopically evaluated on the 7th, 14th, and 21st day after burn induction. Rats treated with the Artaderm ointment had significantly faster wound contraction as well as shorter healing time than the rest groups. No scar was observed in rats treated with the Artaderm ointment on the 21st day, while this level of improvement was not observed in other groups at the same time. More than 90% of wounds were healed after on the 14th day in rats treated with Artaderm (94.10 ± 0.18) and Alpha (92.05 ± 0.23) ointments. According to these findings, it can be concluded that Artaderm herbal ointment can be used as a proper alternative for healing of wounds in second-degree burns.

Antimycobacterial Activity and Gas Chromatography-Mass Spectrometry (GC-MS) Analysis of Henna (Lawsonia Inermis) Leaves extract.

Lawsonia inermis or (henna) is used as a cosmetic dye and medicinal plant for its strong antimycobacterial and other therapeutic effects. In this study, we investigated the phytochemical and antimycobacterial activity of L. inermis leaves methanol extract against Rifampicin sensitive and resistant mycobacterium tuberculosis ( M.tb ) strains. Fresh Sudanese henna leaves were collected from the University of Gezira campus garden, shade dried, ground and was extracted by methanol. Crude henna methanolic extract was then tested for antimycobacterial activity against eight M.tb strains sensitive and resistant to rifampicin, using drug susceptibility test. The phytoconstituents were identified by using gas chromatograph interfaced to a mass spectrometer (GC-MS). GC-MS analysis revealed the presence of seven phytochemical components, with prevailing of squalene (34.433%), Lawsone (20.185%) and Coumarin-4-carboxylic acid (23.328%). The crude methanolic extract of henna leaves demonstrated an inhibitory effect on all tested M.tb strains, with antimycobacterial activity being ≤ 50 mg/ml. Henna leaves crude methanolic extract is rich in phytoconstituents that may involve in its remarkable antimycobacterial activity against rifampicin sensitive and resistant M.tb strains.

Improvement of rheological and filtration characteristics of water-based drilling fluids using naturally derived henna leaf and hibiscus leaf extracts

Biodegradable additives are required to minimize the environmental hazards from drilling fluid wastes. This study explores the feasibility of the applications of henna leaf extracts (HLE) and hibiscus leaf extracts (HBLE) as ecological benign products in water-based drilling fluids (WBDFs). Rheological and filtration characterizations were carried out on the WBDFs to detect the effects of different concentrations (1, 2, 10, 20, 30, and 40 g) of these plant extracts at 78 and 300 °F. The results of 1 and 2 g of the plant extracts were compared with those of low-viscosity polyanionic cellulose (PAC LV). Compatibility test was carried out using 25 g/L of the green additives on base fluid (A-0), and the swelling rate of sodium bentonite in distilled water was also considered using 1, 10, and 20 g of the green additives. The findings showed that HLE and HBLE significantly reduced the filtrate loss between 62% and 67% and between 64% and 76%, respectively, and improved the rheological characteristics of the WBDF system between 10 and 40 g. PAC LV showed a greater effect on the rheological properties than the green additives in equal amounts (1 and 2 g), but it exhibited flat high and progressive gels which can lead to mechanical pipe sticking. The test data also showed that the inclusion of HLE and HBLE in the WBDF demonstrated larger impact on the mud cake than PAC LV. The cake thickness of the WBDF was reduced in the following order: 30–32% (by HLE), 32–33% (by HBLE), and 24–27% (by PAC LV). This interprets the outstanding filtration characteristics of green additives. Further, compatibility test data confirmed that the green additives are compatible with the other base fluid additives and the swelling behavior of sodium bentonite verified that the green plants are effective in inhibiting bentonite swelling. Here, the Henna extracts displayed higher inhibition property than the Hibiscus product. Notwithstanding, both products showed excellent inhibition property and a strong viscosity enhancing effect on the WBDF system.

In Vitro: Cytotoxicity, Apoptosis and Ameliorative Potential of Lawsonia inermis Extract in Human Lung, Colon and Liver Cancer Cell Line

Cancer has emerged as a major public health issue in developed as well as in developing countries. Plant-derived molecules are widely being used in the treatment of cancer due to their minimum side effects. Lawsonia inermis (Henna) is one of the medicinal plants containing many therapeutic properties. In the present study, bioactive components of L. inermis extract were analyzed by LCMS/MS method and validated. Lawsone (3.5%) is primarily responsible for cytotoxic and anti-cancerous activities. These properties were studied on human lung carcinoma (A549), colorectal cancer (DLD1) and Hepatocellular carcinoma (HepG2) cancer cell lines. The activities were assessed by MTT assay, evaluation of apoptosis by measuring the production of Reactive Oxygen Species (ROS) and mitochondrial membrane potential of the cancer cell lines. Moreover, apoptosis in the respective cancer cell lines was also determined by chromatin condensation and DNA fragmentation using Hoechst 33528 and propidium iodide (PI) staining. The preliminary in vitro result of MTT showed that the henna extract induces cytotoxic properties against A549, DLD1, HepG2 with IC50values 490, 480 and 610 μg/ml respectively (more than 40% growth inhibition). In addition, the extract induced a concentration-dependent rise in ROS production which was 84, 102, and 110% in HepG2, DLD1 AND A549 respectively at 300 μg/ml, whereas at 400 μg/ml concentration it was 86, 102, and 106% in respective cell lines while decreasing mitochondrial membrane potential was more than 20% in the investigated cell lines. The extract also provoked changes associated with apoptosis and the data indicate that the ROS production leads to a diminution in mitochondrial membrane potential and this correlated with the extract cytotoxicity.

Microwave-assisted eco-dyeing of bio mordanted silk fabric using cinnamon bark (Cinnamomum Verum) based yellow natural dye

The revival of sustainable plant-based dyes, now a day is becoming the demands of the global community in every field. The current study has been concerned with the exploration of cinnamon bark as a source of yellow natural colorant for silk dyeing. The yellow dye has been extracted in various media and exposed to microwave treatment for up to 6 min. The results show that dyeing of irradiated fabric at 35 °C for 45 min using an irradiated extract of 3 pH containing 1g of salt as a leveling agent has given excellent results. Salt of Al& Fe as sustainable chemical mordants & extract of acacia, henna, rose, pomegranate and turmeric as sustainable bio-mordants employed have not only given new shades but also improved fastness ratings. Conclusively, the microwave treatment has not only enhanced the extraction yield of dye from cinnamon bark but also has made the process more sustainable and ecofriendly by utilizing bio-mordants extracts.

Box-Behnken design to enhance the corrosion resistance of high strength steel alloy in 3.5 wt.% NaCl solution

Recently, the production of green corrosion inhibitors and green inhibition strategies is highly demanded in the field of science and technology due to the growing demand of green chemistry. Usage of plant extracts as metallic corrosion inhibitors has attracted considerable attention in the last few decades. Plant products are perfect green candidates for replacing traditional toxic corrosion inhibitors. Reduced environmental risk, lower cost, widespread availability and high corrosion inhibition efficiency make plant extracts ideal candidates for the substitution of costly and harmful traditional synthetic corrosion inhibitors. An aqueous extract of plant material Henna (Lawsonia Inermis) powder was used as a corrosion inhibitor to control the corrosion of high strength steel immersed in an aqueous solution containing 35000 ppm Cl − , by electrochemical methods in the absence and presence of the inhibitor. Lawsone is the principal constituent of this plant extract. This has excellent efficiency in inhibition and displays excellent IE at neutral pH. Electro-chemical experiments such as potentiodynamic polarization and impedance were used to describe the mechanistic aspects of corrosion inhibitions. Box-Behnken method was used to investigate the effect of key parameters (i.e. concentration of inhibitors, temperature, and speed of rotation) on the efficiency of inhibition and corrosion rate of high strength steel alloy in 3.5 wt.% NaCl solutions. From electrochemical measurements and statistical analysis, it was found that the efficacy of inhibition improves with higher inhibitor concentration, low rotation speed and low temperature. The Lawsonia Inermis extracted demonstrated strong corrosion inhibition in saline media for high strength steel alloy and reached 81.68.

Antimicrobial activity of leaves extracts against bacteria isolated from wound infections

Background: Lawsonia inermis (L. inermis) is perennial plant commonly called henna. It is frequently cultivated in Sudan. Beside its uses cosmetics for staining hands and as hairs dyes‚ it was reported to be useful in jaundice, enlargement of spleen, calculus affliction and skin disease. Method: This descriptive study was done during the period from December 2014 to April 2015 in order to determine the invitro antimicrobial activity of L. inermis (henna) leaves extract against standard and clinical isolates from wound swabs, including Staphylococcus aureus, Escherichia coli, Proteus species, Klebsiella pneumonia, and Pseudomonas aeruginosa. These organisms were collected from different hospitals in Khartoum State including: Soba University Hospital, Military Teaching Hospital, and Laboratory Management Center. The in vitro antimicrobial susceptibly testing was performed using cup plate diffusion method. The activity of L. inermis Linn leaves extract was controlled with four reference antibiotics including gentamicin, oxacillin, ciprofloxacin, and impinim. Results: When aqueous extract of L. inermis Linn examined against standard bacteria and clinical isolates result showed that all standard bacteria were inhibited at 100%, 50%, and 25% concentration. All clinical isolates were successfully inhibited at 100%, 50%, 25%, and 12.5%. In contrary, the activity of methanolic extract of L. inermis Linn against standard bacteria showed that all standard bacteria were inhibited at 100%, 50% concentration, However, the clinical isolates showed an inhibition rate various depending on the concentration of methanolic extract of L. inermis Linn with S. aureus being most sensitive isolate. Conclusion: We conclude that aqueous and methanolic extract of henna exhibited antimicrobial activity against all types of tested organisms both clinical and standard isolates. But the aqueous extract shows superior inhibition ability than the methanolic.

Evaluation of Henna Extract Performance on Corrosion Inhibition of API 5L Steel in H2S-Containing Medium and DFT Quantum Computing of Its Constituents

In this work, the effect of Henna extract as an inhibitor on the corrosion behavior of API 5L Gr.B steel in H2S-containing solution at 25 ˚C was investigated. The electrochemical impedance spectroscopy (EIS) and potentiodynamic polarization tests were used for determination of corrosion parameters in the blank sample and samples with different concentrations of the extract. The obtained results indicated that with increasing Henna extract concentration, the corrosion current density decreased and the inhibition efficiency increased. The calculated efficiency of EIS and polarization tests showed good agreement. The studied inhibitor in this work follows the Langmuir adsorption isotherm and the type of surface adsorption is physical. Quantum chemical parameters such as energy gap ( $$\Delta{\text{E}}$$ = EHOMO – ELUMO), lowest unoccupied molecular orbital energy (ELUMO), highest occupied molecular orbital energy (EHOMO), hardness ( $$\eta$$ ) and electronegativity ( $${\chi}_{\text{inh}}$$ ) for the main constituents of Henna extract were calculated. The results of quantum chemistry proved that the main constituent of Henna extract and the inhibition agent was Lawsone.

Formulation, Physiochemical, and Microbial Assay of Henna Oil Vaginal Suppository Formulated with Polyethylene Glycol Bases.

Background: Persian Medicine is one of the oldest and richest complementary and alternative options in the field of medicine and has a comprehensive medical system. Henna oil is recommended in Persian Medicine for the treatment of numerous women’s diseases such as cervicitis. To date, henna has been used for many medical purposes, including astringent, bleeding, cardioinhibitory, hypotension, and relaxation. Accordingly, the present study aimed to provide the formulation of a henna-oil-based vaginal suppository and examine its physicochemical and antimicrobial properties. Methods: The present study was approved and performed in accordance with the regulations of Research Council, Kerman University of Medical Sciences, Kerman, Iran, in July 2016. Different percentages of henna oil, glycerin, and gelatin, as well as henna oil and polyethylene glycol 400 and 4000, were mixed to achieve a formulation with proper appearance features and, particularly, without any oil leakage from the suppository surface. Uniformity of weight, uniformity of content, disintegration time, and dissolution test of the suppositories were evaluated. The growth-inhibiting activity of the suppositories and aqueous extract of henna was evaluated against bacteria, including the Gram-positive bacterium Gardnerella vaginalis, Neisseria gonorrhoeae, and group B streptococcus. Results: The formulations had a smooth appearance without any cracks or fractures. Disintegration times for glycero-gelatin and polyethylene glycol suppositories were 60 and 10 min, respectively. 40% of the drug was released from polyethylene glycol suppositories after 60 min, but glycero-gelatin suppositories had no release after three hours. Minimum inhibitory concentration (MIC) of suppositories and aqueous extract were 0.4 mg/mL and 0.01 mg/mL, respectively. Conclusion: Polyethylene glycol suppositories had acceptable physicochemical properties, and the henna extract and suppositories inhibited the three studied pathogens.

Study the dyeing behavior of wool fabric using henna extract in decamethyl cyclopentasiloxane (D5) medium

In this study, an eco-friendly dyeing method of wool fabric was adopted using natural dye extracted from henna leaves in the Decamethyl Cyclopentasiloxane (D5) medium. D5 was used to reduce the consumption of the high amount of water and salt needed in traditional dyeing. Wool fabric dyed with different amounts of Henna extract/Decamethyl Cyclopentasiloxane at different dyeing temperatures (40 and Scanning electron microscopy (SEM) and Energy-dispersive X-ray spectroscopy (EDX) were used to study the effect of the D5 on the morphology and elemental composition of the fiber surface. The dyeing and fastness properties of the dyed fabric were also investigated. The results showed that the dye uptake, fixation rate, and color strength of the samples dyed in this system were higher than water-based dyed samples. The dye uptake rates of the samples dyed in this system were 77–83% as compared with 64–71% for the water-based dyed samples. SEM and EDX results illustrated that dyeing in the D5 medium did not show any adverse effect on the morphology and elemental composition of the fiber surface.

ALTERNATIVE STAINING OF FASCIOLA GIGANTICA USING NATURAL HERBAL DYES

The Aqueous extracts of red beet, China rose, henna and acid carmine stains were used to stain the adult worms of F. gigantca. The stained flukes showed varying degrees of pigmentation with well-defined external and internal structure. Oral and ventral sucker, muscular pharynx, oesophagus, intestinal ceca and cirrus sac took rose to light cherry color in aqueous extract of china rose, orange to brown color in aqueous extract of sugar beet, beige to pale brown color in aqueous extract of henna while they took deep red color in carmine stain with best quality achieved by china rose aqueous extract. The ovary, testes and longitudinal excretory canal/pore took rose to cherry color in aqueous extract of China rose, pale brown color in aqueous extracts of both sugar beet and henna while they acquired red to pink color in acid carmine stain. Aqueous extracts of sugar beet exhibited little better quality in revealing characteristics of vitelline ducts andvitelline glands compared to the aqueous extract of China rose, henna and carmine stain. Uterus stained brown in both sugar beet aqueous extracts and carmine stain while it took yellow color when stained with China rose and henna. Eggs inside uterus took light yellow color in all tested aqueous extracts. Ootype/mehli gland and yolk reservoir of F. gigantica took brown color in aqueous extracts of red beet, China rose and henna, red to pink color in carmine stain. Spinescovering the cuticle appeared rose color in aqueous extract of china rose, pale brown color in aqueous extract of sugar beet extract and henna, and pale red color in carmine stain.

Synergistic Effect of Different Plant Extracts and Antibiotics on Some Pathogenic Bacteria

In this study, the antibacterial activity of methanol extract of henna (Lawsonia inermis) leaves, ethanol extract of pomegranate (Punica granatum) peel, volatile oil of sesame (Sesamum indicum) and peanut (Arachis hypogaea) were investigated against some Gram-positive and Gram-negative bacteria includingStaphylococcus aureus, Bacillus cereus, Escherichia coli and Acinetobacter sp. Henna extract was most effective substrate against all tested bacteria followed by pomegranate and peanut while sesame was less effective. All extracts were screened for their antibacterial activity in combination with commonly used antibiotics, including ciprofloxacin and erythromycin to evaluate synergistic effects using Minimum inhibitory concentrations (MIC) method which determined by microbroth dilution assays. Different interactions (synergistic and indifference) were observed between plant extracts and used antibiotics. The fractional inhibitory concentration (FIC) index ranged from 0.01 to 1.25 for B. cereus, 0.5 to 1 for P. aeruginosa, 0.01 to 0.3 for S. aureus and 0.06 to 0.25 for A. baumannii. The best synergistic capacity appeared between erythromycin and sesame. In vitro interaction between antimicrobial agents in combination with tested plant extracts showed synergistic effects. The MICs of each antibiotic was decreased to half when it is used in combination with tested plant extracts. This decreasing in MICs was observed in all plant extracts against tested bacteria as well as the extracts exhibited weak antibacterial activity alone.