Antimicrobial activity of leaves extracts against bacteria isolated from wound infections

Goris Bmt,Sabahalkheir Kg,Ibrahim Lo,Ibrahim Aa,Ishaq Mm

doi:10.17727/jmsr.2020/8-16

Abstract

Background: Lawsonia inermis (L. inermis) is perennial plant commonly called henna. It is frequently cultivated in Sudan. Beside its uses cosmetics for staining hands and as hairs dyes‚ it was reported to be useful in jaundice, enlargement of spleen, calculus affliction and skin disease. Method: This descriptive study was done during the period from December 2014 to April 2015 in order to determine the invitro antimicrobial activity of L. inermis (henna) leaves extract against standard and clinical isolates from wound swabs, including Staphylococcus aureus, Escherichia coli, Proteus species, Klebsiella pneumonia, and Pseudomonas aeruginosa. These organisms were collected from different hospitals in Khartoum State including: Soba University Hospital, Military Teaching Hospital, and Laboratory Management Center. The in vitro antimicrobial susceptibly testing was performed using cup plate diffusion method. The activity of L. inermis Linn leaves extract was controlled with four reference antibiotics including gentamicin, oxacillin, ciprofloxacin, and impinim. Results: When aqueous extract of L. inermis Linn examined against standard bacteria and clinical isolates result showed that all standard bacteria were inhibited at 100%, 50%, and 25% concentration. All clinical isolates were successfully inhibited at 100%, 50%, 25%, and 12.5%. In contrary, the activity of methanolic extract of L. inermis Linn against standard bacteria showed that all standard bacteria were inhibited at 100%, 50% concentration, However, the clinical isolates showed an inhibition rate various depending on the concentration of methanolic extract of L. inermis Linn with S. aureus being most sensitive isolate. Conclusion: We conclude that aqueous and methanolic extract of henna exhibited antimicrobial activity against all types of tested organisms both clinical and standard isolates. But the aqueous extract shows superior inhibition ability than the methanolic.

Highlights

Lawsonia inermis (L. inermis) is a glabrous branched small tree, 2-6 m in height
When aqueous extract of L. inermis Linn examined against standard bacteria and clinical isolates result showed that all standard bacteria were inhibited at 100%, 50%, and 25% concentration
The activity of methanolic extract of L. inermis Linn against standard bacteria showed that all standard bacteria were inhibited at 100%, 50% concentration, the clinical isolates showed an inhibition rate various depending on the concentration of methanolic extract of L. inermis Linn with S. aureus being most sensitive isolate

Summary

Introduction

Lawsonia inermis (L. inermis) is a glabrous branched small tree, 2-6 m in height. L. inermis is perennial plant commonly called henna. Often cultivated in North Africa, south East Asia, India, Persia, and along the African coast of Mediterranean Sea. Henna roots may helpful in management of the burning sensation, leprosy, skin disease, a menorrhea, a bortifacient, bitter, and premature graying of hair [6]. Wound is breach in the skin and exposure of subcutaneous tissue following loss of integrity which provides a moist, warm, and nutritive environment that is conductive for microbial colonization and proliferation [7,8,9]. Lawsonia inermis (L. inermis) is perennial plant commonly called henna. Beside its uses cosmetics for staining hands and as hairs dyes‚ it was reported to be useful in jaundice, enlargement of spleen, calculus affliction and skin disease

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Conclusion